This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.66 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 36,060 (1.4%) Reads that were too short: 7,863 (0.3%) Reads written (passing filters): 28,197 (1.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 6,248,305 bp (0.8%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 36060 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 332 621.6 0 332 7 4501 621.6 0 4501 8 149 621.6 0 149 9 4405 621.6 0 4405 10 11 621.6 0 11 11 9 621.6 0 9 12 6 621.6 0 6 13 10 621.6 0 10 14 3 621.6 0 3 15 11 621.6 0 11 16 2 621.6 0 2 17 1 621.6 0 1 18 2 621.6 0 2 19 4 621.6 0 4 20 1 621.6 0 1 21 4 621.6 0 4 22 10 621.6 0 10 23 19 621.6 0 19 24 16 621.6 0 16 25 92 621.6 0 92 26 25 621.6 0 25 27 24 621.6 0 24 28 9 621.6 0 9 29 5 621.6 0 5 30 6 621.6 0 6 31 3 621.6 0 3 32 12 621.6 0 12 33 29 621.6 0 29 34 85 621.6 0 85 35 176 621.6 0 176 36 4 621.6 0 4 37 6 621.6 0 6 38 4 621.6 0 4 39 5 621.6 0 5 40 12 621.6 0 12 41 3 621.6 0 3 42 4 621.6 0 4 43 4 621.6 0 4 44 4 621.6 0 4 45 1 621.6 0 1 46 9 621.6 0 9 47 6 621.6 0 6 48 4 621.6 0 4 49 7 621.6 0 7 50 3 621.6 0 3 51 5 621.6 0 5 52 6 621.6 0 6 53 6 621.6 0 6 54 1 621.6 0 1 55 3 621.6 0 3 56 5 621.6 0 5 57 7 621.6 0 7 58 6 621.6 0 6 59 5 621.6 0 5 60 11 621.6 0 11 61 3 621.6 0 3 62 8 621.6 0 8 63 6 621.6 0 6 64 10 621.6 0 10 65 7 621.6 0 7 66 11 621.6 0 11 67 3 621.6 0 3 68 8 621.6 0 8 69 2 621.6 0 2 71 3 621.6 0 3 72 1 621.6 0 1 73 10 621.6 0 10 74 9 621.6 0 9 75 19 621.6 0 19 76 66 621.6 0 66 77 31 621.6 0 31 78 42 621.6 0 42 79 62 621.6 0 62 80 22 621.6 0 22 81 43 621.6 0 43 82 29 621.6 0 29 83 26 621.6 0 26 84 7 621.6 0 7 85 18 621.6 0 18 86 33 621.6 0 33 87 25 621.6 0 25 88 30 621.6 0 30 89 56 621.6 0 56 90 19 621.6 0 19 91 9 621.6 0 9 92 16 621.6 0 16 93 21 621.6 0 21 94 24 621.6 0 24 95 62 621.6 0 62 96 37 621.6 0 37 97 106 621.6 0 106 98 145 621.6 0 145 99 1227 621.6 0 1227 100 472 621.6 0 472 101 868 621.6 0 868 102 83 621.6 0 83 103 35 621.6 0 35 104 35 621.6 0 35 105 34 621.6 0 34 106 60 621.6 0 60 107 42 621.6 0 42 108 56 621.6 0 56 109 96 621.6 0 96 110 127 621.6 0 127 111 1615 621.6 0 1615 112 1251 621.6 0 1251 113 3719 621.6 0 3719 114 371 621.6 0 371 115 2698 621.6 0 2698 116 19 621.6 0 19 117 8 621.6 0 8 118 13 621.6 0 13 119 21 621.6 0 21 120 20 621.6 0 20 121 21 621.6 0 21 122 10 621.6 0 10 123 11 621.6 0 11 124 10 621.6 0 10 125 17 621.6 0 17 126 16 621.6 0 16 127 34 621.6 0 34 128 18 621.6 0 18 129 55 621.6 0 55 130 82 621.6 0 82 131 128 621.6 0 128 132 81 621.6 0 81 133 63 621.6 0 63 134 50 621.6 0 50 135 120 621.6 0 120 136 82 621.6 0 82 137 231 621.6 0 231 138 26 621.6 0 26 139 28 621.6 0 28 140 25 621.6 0 25 141 30 621.6 0 30 142 116 621.6 0 116 143 406 621.6 0 406 144 455 621.6 0 455 145 603 621.6 0 603 146 53 621.6 0 53 147 61 621.6 0 61 148 24 621.6 0 24 149 32 621.6 0 32 150 31 621.6 0 31 151 33 621.6 0 33 152 17 621.6 0 17 153 22 621.6 0 22 154 18 621.6 0 18 155 15 621.6 0 15 156 21 621.6 0 21 157 19 621.6 0 19 158 31 621.6 0 31 159 29 621.6 0 29 160 44 621.6 0 44 161 23 621.6 0 23 162 48 621.6 0 48 163 38 621.6 0 38 164 31 621.6 0 31 165 33 621.6 0 33 166 43 621.6 0 43 167 27 621.6 0 27 168 25 621.6 0 25 169 22 621.6 0 22 170 17 621.6 0 17 171 21 621.6 0 21 172 21 621.6 0 21 173 15 621.6 0 15 174 17 621.6 0 17 175 31 621.6 0 31 176 24 621.6 0 24 177 41 621.6 0 41 178 40 621.6 0 40 179 23 621.6 0 23 180 27 621.6 0 27 181 24 621.6 0 24 182 24 621.6 0 24 183 14 621.6 0 14 184 28 621.6 0 28 185 21 621.6 0 21 186 19 621.6 0 19 187 27 621.6 0 27 188 24 621.6 0 24 189 20 621.6 0 20 190 23 621.6 0 23 191 14 621.6 0 14 192 15 621.6 0 15 193 32 621.6 0 32 194 20 621.6 0 20 195 22 621.6 0 22 196 24 621.6 0 24 197 19 621.6 0 19 198 20 621.6 0 20 199 32 621.6 0 32 200 25 621.6 0 25 201 23 621.6 0 23 202 49 621.6 0 49 203 46 621.6 0 46 204 40 621.6 0 40 205 21 621.6 0 21 206 24 621.6 0 24 207 27 621.6 0 27 208 28 621.6 0 28 209 40 621.6 0 40 210 38 621.6 0 38 211 28 621.6 0 28 212 26 621.6 0 26 213 27 621.6 0 27 214 28 621.6 0 28 215 39 621.6 0 39 216 42 621.6 0 42 217 37 621.6 0 37 218 35 621.6 0 35 219 38 621.6 0 38 220 39 621.6 0 39 221 41 621.6 0 41 222 53 621.6 0 53 223 54 621.6 0 54 224 66 621.6 0 66 225 42 621.6 0 42 226 97 621.6 0 97 227 38 621.6 0 38 228 27 621.6 0 27 229 31 621.6 0 31 230 40 621.6 0 40 231 25 621.6 0 25 232 36 621.6 0 36 233 45 621.6 0 45 234 60 621.6 0 60 235 43 621.6 0 43 236 45 621.6 0 45 237 33 621.6 0 33 238 41 621.6 0 41 239 71 621.6 0 71 240 45 621.6 0 45 241 85 621.6 0 85 242 98 621.6 0 98 243 94 621.6 0 94 244 53 621.6 0 53 245 43 621.6 0 43 246 31 621.6 0 31 247 28 621.6 0 28 248 39 621.6 0 39 249 30 621.6 0 30 250 40 621.6 0 40 251 29 621.6 0 29 252 92 621.6 0 92 253 42 621.6 0 42 254 63 621.6 0 63 255 49 621.6 0 49 256 75 621.6 0 75 257 79 621.6 0 79 258 63 621.6 0 63 259 39 621.6 0 39 260 53 621.6 0 53 261 54 621.6 0 54 262 130 621.6 0 130 263 152 621.6 0 152 264 177 621.6 0 177 265 92 621.6 0 92 266 41 621.6 0 41 267 35 621.6 0 35 268 39 621.6 0 39 269 43 621.6 0 43 270 57 621.6 0 57 271 66 621.6 0 66 272 60 621.6 0 60 273 76 621.6 0 76 274 38 621.6 0 38 275 103 621.6 0 103 276 118 621.6 0 118 277 167 621.6 0 167 278 111 621.6 0 111 279 53 621.6 0 53 280 54 621.6 0 54 281 71 621.6 0 71 282 199 621.6 0 199 283 389 621.6 0 389 284 678 621.6 0 678 285 363 621.6 0 363 286 47 621.6 0 47 287 50 621.6 0 50 288 49 621.6 0 49 289 55 621.6 0 55 290 85 621.6 0 85 291 156 621.6 0 156 292 200 621.6 0 200 293 182 621.6 0 182 294 296 621.6 0 296 295 225 621.6 0 225 296 135 621.6 0 135 297 62 621.6 0 62 298 75 621.6 0 75 299 58 621.6 0 58 300 64 621.6 0 64 301 48 621.6 0 48 Finished in 1.23 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 28,197 Reads with adapters: 4,826 (17.1%) Reads that were too short: 66 (0.2%) Reads written (passing filters): 4,760 (16.9%) Total basepairs processed: 6,248,305 bp Total written (filtered): 1,290,256 bp (20.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4826 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 5 3 16 117 0.0 3 0 101 10 6 17 4439 0.0 3 4046 317 63 13 18 46 0.0 3 14 22 5 5 19 10 0.0 3 1 1 7 1 20 14 0.0 3 4 2 0 8 21 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 26 0.0 3 0 0 0 26 32 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 5 0.0 3 0 0 0 5 55 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 72 16 0.0 3 0 0 1 15 73 5 0.0 3 0 0 0 5 75 5 0.0 3 0 0 0 5 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 12 0.0 3 0 0 0 12 93 6 0.0 3 0 0 0 6 94 1 0.0 3 0 0 0 1 95 5 0.0 3 0 0 0 5 96 4 0.0 3 0 0 0 4 97 3 0.0 3 0 0 0 3 98 5 0.0 3 0 0 0 5 99 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 4 0.0 3 0 0 1 3 116 2 0.0 3 0 0 0 2 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 142 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 5 0.0 3 0 0 0 5 145 2 0.0 3 0 0 0 2 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 5 0.0 3 0 0 2 3 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 4,760 Reads with adapters: 61 (1.3%) Reads written (passing filters): 4,760 (100.0%) Total basepairs processed: 1,290,256 bp Total written (filtered): 1,290,060 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 61 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.7% C: 16.4% G: 23.0% T: 4.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 50 74.4 0 50 4 9 18.6 0 9 5 2 4.6 0 2