This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.65 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 30,682 (1.2%) Reads that were too short: 5,463 (0.2%) Reads written (passing filters): 25,219 (1.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 5,694,407 bp (0.7%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 30682 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 335 621.6 0 335 7 5415 621.6 0 5415 8 123 621.6 0 123 9 4019 621.6 0 4019 10 1 621.6 0 1 11 1 621.6 0 1 12 3 621.6 0 3 14 1 621.6 0 1 15 1 621.6 0 1 16 1 621.6 0 1 17 1 621.6 0 1 18 1 621.6 0 1 19 1 621.6 0 1 21 5 621.6 0 5 22 1 621.6 0 1 23 1 621.6 0 1 24 1 621.6 0 1 25 1 621.6 0 1 27 1 621.6 0 1 28 3 621.6 0 3 29 2 621.6 0 2 30 3 621.6 0 3 32 1 621.6 0 1 33 1 621.6 0 1 34 2 621.6 0 2 35 5 621.6 0 5 36 1 621.6 0 1 37 4 621.6 0 4 38 1 621.6 0 1 39 4 621.6 0 4 40 11 621.6 0 11 41 15 621.6 0 15 42 4 621.6 0 4 43 3 621.6 0 3 44 1 621.6 0 1 45 2 621.6 0 2 46 2 621.6 0 2 47 3 621.6 0 3 48 1 621.6 0 1 49 4 621.6 0 4 50 8 621.6 0 8 51 3 621.6 0 3 52 3 621.6 0 3 53 2 621.6 0 2 54 2 621.6 0 2 56 2 621.6 0 2 57 1 621.6 0 1 58 3 621.6 0 3 60 1 621.6 0 1 61 2 621.6 0 2 62 6 621.6 0 6 64 3 621.6 0 3 65 1 621.6 0 1 66 2 621.6 0 2 67 1 621.6 0 1 68 10 621.6 0 10 69 4 621.6 0 4 70 3 621.6 0 3 71 3 621.6 0 3 72 4 621.6 0 4 73 4 621.6 0 4 74 4 621.6 0 4 75 6 621.6 0 6 76 2 621.6 0 2 77 6 621.6 0 6 78 12 621.6 0 12 79 8 621.6 0 8 80 1 621.6 0 1 81 13 621.6 0 13 82 10 621.6 0 10 83 7 621.6 0 7 84 8 621.6 0 8 85 7 621.6 0 7 86 20 621.6 0 20 87 20 621.6 0 20 88 16 621.6 0 16 89 9 621.6 0 9 90 15 621.6 0 15 91 14 621.6 0 14 92 21 621.6 0 21 93 23 621.6 0 23 94 25 621.6 0 25 95 17 621.6 0 17 96 34 621.6 0 34 97 46 621.6 0 46 98 70 621.6 0 70 99 457 621.6 0 457 100 226 621.6 0 226 101 230 621.6 0 230 102 48 621.6 0 48 103 29 621.6 0 29 104 48 621.6 0 48 105 34 621.6 0 34 106 50 621.6 0 50 107 46 621.6 0 46 108 57 621.6 0 57 109 69 621.6 0 69 110 125 621.6 0 125 111 1690 621.6 0 1690 112 1231 621.6 0 1231 113 3885 621.6 0 3885 114 379 621.6 0 379 115 2756 621.6 0 2756 116 13 621.6 0 13 117 6 621.6 0 6 118 11 621.6 0 11 119 17 621.6 0 17 120 9 621.6 0 9 121 15 621.6 0 15 122 6 621.6 0 6 123 7 621.6 0 7 124 7 621.6 0 7 125 14 621.6 0 14 126 9 621.6 0 9 127 11 621.6 0 11 128 14 621.6 0 14 129 43 621.6 0 43 130 44 621.6 0 44 131 85 621.6 0 85 132 39 621.6 0 39 133 32 621.6 0 32 134 16 621.6 0 16 135 81 621.6 0 81 136 61 621.6 0 61 137 225 621.6 0 225 138 16 621.6 0 16 139 25 621.6 0 25 140 27 621.6 0 27 141 23 621.6 0 23 142 106 621.6 0 106 143 474 621.6 0 474 144 483 621.6 0 483 145 593 621.6 0 593 146 17 621.6 0 17 147 27 621.6 0 27 148 9 621.6 0 9 149 16 621.6 0 16 150 24 621.6 0 24 151 13 621.6 0 13 152 12 621.6 0 12 153 15 621.6 0 15 154 17 621.6 0 17 155 10 621.6 0 10 156 11 621.6 0 11 157 17 621.6 0 17 158 14 621.6 0 14 159 18 621.6 0 18 160 16 621.6 0 16 161 15 621.6 0 15 162 22 621.6 0 22 163 19 621.6 0 19 164 14 621.6 0 14 165 15 621.6 0 15 166 13 621.6 0 13 167 17 621.6 0 17 168 16 621.6 0 16 169 13 621.6 0 13 170 12 621.6 0 12 171 12 621.6 0 12 172 13 621.6 0 13 173 11 621.6 0 11 174 15 621.6 0 15 175 9 621.6 0 9 176 11 621.6 0 11 177 22 621.6 0 22 178 26 621.6 0 26 179 17 621.6 0 17 180 24 621.6 0 24 181 17 621.6 0 17 182 11 621.6 0 11 183 6 621.6 0 6 184 14 621.6 0 14 185 20 621.6 0 20 186 23 621.6 0 23 187 16 621.6 0 16 188 11 621.6 0 11 189 27 621.6 0 27 190 15 621.6 0 15 191 15 621.6 0 15 192 10 621.6 0 10 193 19 621.6 0 19 194 18 621.6 0 18 195 11 621.6 0 11 196 17 621.6 0 17 197 22 621.6 0 22 198 13 621.6 0 13 199 15 621.6 0 15 200 10 621.6 0 10 201 17 621.6 0 17 202 22 621.6 0 22 203 23 621.6 0 23 204 13 621.6 0 13 205 15 621.6 0 15 206 20 621.6 0 20 207 9 621.6 0 9 208 27 621.6 0 27 209 17 621.6 0 17 210 19 621.6 0 19 211 24 621.6 0 24 212 18 621.6 0 18 213 16 621.6 0 16 214 19 621.6 0 19 215 18 621.6 0 18 216 21 621.6 0 21 217 28 621.6 0 28 218 18 621.6 0 18 219 22 621.6 0 22 220 20 621.6 0 20 221 27 621.6 0 27 222 40 621.6 0 40 223 36 621.6 0 36 224 40 621.6 0 40 225 31 621.6 0 31 226 93 621.6 0 93 227 12 621.6 0 12 228 11 621.6 0 11 229 22 621.6 0 22 230 25 621.6 0 25 231 18 621.6 0 18 232 23 621.6 0 23 233 24 621.6 0 24 234 39 621.6 0 39 235 21 621.6 0 21 236 22 621.6 0 22 237 19 621.6 0 19 238 27 621.6 0 27 239 31 621.6 0 31 240 19 621.6 0 19 241 49 621.6 0 49 242 60 621.6 0 60 243 51 621.6 0 51 244 36 621.6 0 36 245 29 621.6 0 29 246 24 621.6 0 24 247 22 621.6 0 22 248 25 621.6 0 25 249 23 621.6 0 23 250 24 621.6 0 24 251 22 621.6 0 22 252 42 621.6 0 42 253 29 621.6 0 29 254 35 621.6 0 35 255 35 621.6 0 35 256 39 621.6 0 39 257 46 621.6 0 46 258 34 621.6 0 34 259 41 621.6 0 41 260 35 621.6 0 35 261 34 621.6 0 34 262 76 621.6 0 76 263 59 621.6 0 59 264 73 621.6 0 73 265 42 621.6 0 42 266 22 621.6 0 22 267 23 621.6 0 23 268 37 621.6 0 37 269 34 621.6 0 34 270 28 621.6 0 28 271 25 621.6 0 25 272 32 621.6 0 32 273 40 621.6 0 40 274 22 621.6 0 22 275 57 621.6 0 57 276 43 621.6 0 43 277 118 621.6 0 118 278 56 621.6 0 56 279 33 621.6 0 33 280 29 621.6 0 29 281 54 621.6 0 54 282 149 621.6 0 149 283 266 621.6 0 266 284 296 621.6 0 296 285 183 621.6 0 183 286 31 621.6 0 31 287 41 621.6 0 41 288 39 621.6 0 39 289 60 621.6 0 60 290 125 621.6 0 125 291 182 621.6 0 182 292 276 621.6 0 276 293 147 621.6 0 147 294 393 621.6 0 393 295 257 621.6 0 257 296 176 621.6 0 176 297 74 621.6 0 74 298 47 621.6 0 47 299 58 621.6 0 58 300 40 621.6 0 40 301 36 621.6 0 36 Finished in 1.20 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 25,219 Reads with adapters: 5,710 (22.6%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 5,698 (22.6%) Total basepairs processed: 5,694,407 bp Total written (filtered): 1,559,761 bp (27.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5710 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 33 0.0 3 0 33 20 5594 0.0 4 5499 90 4 1 21 48 0.0 4 38 10 23 8 0.0 4 3 1 0 3 1 28 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 35 8 0.0 4 1 0 0 0 7 46 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 5 0.0 4 0 0 0 0 5 128 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,698 Reads with adapters: 132 (2.3%) Reads written (passing filters): 5,698 (100.0%) Total basepairs processed: 1,559,761 bp Total written (filtered): 1,557,516 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 132 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.8% C: 4.5% G: 86.4% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 89.0 0 6 4 1 22.3 0 1 10 1 0.0 1 0 1 11 105 0.0 1 1 104 12 13 0.0 1 0 13 19 1 0.0 1 0 1 140 2 0.0 1 2 152 1 0.0 1 0 1 225 1 0.0 1 1 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.