This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.70 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 53,135 (2.1%) Reads that were too short: 17,563 (0.7%) Reads written (passing filters): 35,572 (1.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 7,315,271 bp (1.0%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 53135 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1038 621.6 0 1038 7 3511 621.6 0 3511 8 161 621.6 0 161 9 3228 621.6 0 3228 10 46 621.6 0 46 11 44 621.6 0 44 12 65 621.6 0 65 13 83 621.6 0 83 14 62 621.6 0 62 15 63 621.6 0 63 16 62 621.6 0 62 17 69 621.6 0 69 18 75 621.6 0 75 19 39 621.6 0 39 20 36 621.6 0 36 21 37 621.6 0 37 22 51 621.6 0 51 23 44 621.6 0 44 24 38 621.6 0 38 25 48 621.6 0 48 26 47 621.6 0 47 27 62 621.6 0 62 28 77 621.6 0 77 29 84 621.6 0 84 30 58 621.6 0 58 31 64 621.6 0 64 32 57 621.6 0 57 33 128 621.6 0 128 34 48 621.6 0 48 35 49 621.6 0 49 36 76 621.6 0 76 37 43 621.6 0 43 38 83 621.6 0 83 39 79 621.6 0 79 40 68 621.6 0 68 41 81 621.6 0 81 42 55 621.6 0 55 43 94 621.6 0 94 44 190 621.6 0 190 45 166 621.6 0 166 46 261 621.6 0 261 47 48 621.6 0 48 48 51 621.6 0 51 49 48 621.6 0 48 50 68 621.6 0 68 51 84 621.6 0 84 52 61 621.6 0 61 53 84 621.6 0 84 54 59 621.6 0 59 55 41 621.6 0 41 56 65 621.6 0 65 57 98 621.6 0 98 58 602 621.6 0 602 59 343 621.6 0 343 60 313 621.6 0 313 61 59 621.6 0 59 62 52 621.6 0 52 63 41 621.6 0 41 64 57 621.6 0 57 65 49 621.6 0 49 66 65 621.6 0 65 67 67 621.6 0 67 68 62 621.6 0 62 69 56 621.6 0 56 70 64 621.6 0 64 71 72 621.6 0 72 72 72 621.6 0 72 73 121 621.6 0 121 74 58 621.6 0 58 75 87 621.6 0 87 76 50 621.6 0 50 77 82 621.6 0 82 78 52 621.6 0 52 79 54 621.6 0 54 80 58 621.6 0 58 81 63 621.6 0 63 82 63 621.6 0 63 83 86 621.6 0 86 84 71 621.6 0 71 85 75 621.6 0 75 86 69 621.6 0 69 87 61 621.6 0 61 88 54 621.6 0 54 89 64 621.6 0 64 90 74 621.6 0 74 91 107 621.6 0 107 92 101 621.6 0 101 93 117 621.6 0 117 94 97 621.6 0 97 95 97 621.6 0 97 96 67 621.6 0 67 97 100 621.6 0 100 98 101 621.6 0 101 99 88 621.6 0 88 100 54 621.6 0 54 101 67 621.6 0 67 102 67 621.6 0 67 103 61 621.6 0 61 104 69 621.6 0 69 105 106 621.6 0 106 106 456 621.6 0 456 107 269 621.6 0 269 108 419 621.6 0 419 109 104 621.6 0 104 110 91 621.6 0 91 111 69 621.6 0 69 112 74 621.6 0 74 113 80 621.6 0 80 114 203 621.6 0 203 115 145 621.6 0 145 116 141 621.6 0 141 117 71 621.6 0 71 118 78 621.6 0 78 119 85 621.6 0 85 120 54 621.6 0 54 121 83 621.6 0 83 122 60 621.6 0 60 123 101 621.6 0 101 124 68 621.6 0 68 125 112 621.6 0 112 126 109 621.6 0 109 127 161 621.6 0 161 128 1787 621.6 0 1787 129 884 621.6 0 884 130 990 621.6 0 990 131 98 621.6 0 98 132 92 621.6 0 92 133 104 621.6 0 104 134 192 621.6 0 192 135 181 621.6 0 181 136 321 621.6 0 321 137 216 621.6 0 216 138 266 621.6 0 266 139 76 621.6 0 76 140 73 621.6 0 73 141 266 621.6 0 266 142 126 621.6 0 126 143 219 621.6 0 219 144 153 621.6 0 153 145 89 621.6 0 89 146 88 621.6 0 88 147 162 621.6 0 162 148 1783 621.6 0 1783 149 1175 621.6 0 1175 150 1300 621.6 0 1300 151 67 621.6 0 67 152 52 621.6 0 52 153 107 621.6 0 107 154 79 621.6 0 79 155 370 621.6 0 370 156 192 621.6 0 192 157 280 621.6 0 280 158 218 621.6 0 218 159 58 621.6 0 58 160 44 621.6 0 44 161 121 621.6 0 121 162 144 621.6 0 144 163 559 621.6 0 559 164 314 621.6 0 314 165 366 621.6 0 366 166 64 621.6 0 64 167 50 621.6 0 50 168 87 621.6 0 87 169 64 621.6 0 64 170 93 621.6 0 93 171 31 621.6 0 31 172 42 621.6 0 42 173 50 621.6 0 50 174 57 621.6 0 57 175 50 621.6 0 50 176 43 621.6 0 43 177 67 621.6 0 67 178 67 621.6 0 67 179 102 621.6 0 102 180 324 621.6 0 324 181 236 621.6 0 236 182 267 621.6 0 267 183 194 621.6 0 194 184 58 621.6 0 58 185 95 621.6 0 95 186 61 621.6 0 61 187 48 621.6 0 48 188 44 621.6 0 44 189 69 621.6 0 69 190 63 621.6 0 63 191 75 621.6 0 75 192 79 621.6 0 79 193 93 621.6 0 93 194 63 621.6 0 63 195 49 621.6 0 49 196 63 621.6 0 63 197 61 621.6 0 61 198 58 621.6 0 58 199 54 621.6 0 54 200 55 621.6 0 55 201 59 621.6 0 59 202 132 621.6 0 132 203 89 621.6 0 89 204 154 621.6 0 154 205 45 621.6 0 45 206 38 621.6 0 38 207 43 621.6 0 43 208 75 621.6 0 75 209 89 621.6 0 89 210 71 621.6 0 71 211 57 621.6 0 57 212 74 621.6 0 74 213 109 621.6 0 109 214 212 621.6 0 212 215 269 621.6 0 269 216 256 621.6 0 256 217 100 621.6 0 100 218 56 621.6 0 56 219 44 621.6 0 44 220 53 621.6 0 53 221 71 621.6 0 71 222 92 621.6 0 92 223 90 621.6 0 90 224 112 621.6 0 112 225 87 621.6 0 87 226 80 621.6 0 80 227 77 621.6 0 77 228 74 621.6 0 74 229 70 621.6 0 70 230 61 621.6 0 61 231 69 621.6 0 69 232 96 621.6 0 96 233 151 621.6 0 151 234 194 621.6 0 194 235 149 621.6 0 149 236 85 621.6 0 85 237 73 621.6 0 73 238 77 621.6 0 77 239 82 621.6 0 82 240 56 621.6 0 56 241 73 621.6 0 73 242 96 621.6 0 96 243 91 621.6 0 91 244 112 621.6 0 112 245 73 621.6 0 73 246 69 621.6 0 69 247 127 621.6 0 127 248 122 621.6 0 122 249 81 621.6 0 81 250 56 621.6 0 56 251 79 621.6 0 79 252 90 621.6 0 90 253 170 621.6 0 170 254 116 621.6 0 116 255 144 621.6 0 144 256 99 621.6 0 99 257 113 621.6 0 113 258 122 621.6 0 122 259 94 621.6 0 94 260 124 621.6 0 124 261 1095 621.6 0 1095 262 519 621.6 0 519 263 749 621.6 0 749 264 112 621.6 0 112 265 82 621.6 0 82 266 94 621.6 0 94 267 93 621.6 0 93 268 74 621.6 0 74 269 122 621.6 0 122 270 163 621.6 0 163 271 836 621.6 0 836 272 376 621.6 0 376 273 553 621.6 0 553 274 107 621.6 0 107 275 70 621.6 0 70 276 70 621.6 0 70 277 77 621.6 0 77 278 118 621.6 0 118 279 184 621.6 0 184 280 165 621.6 0 165 281 131 621.6 0 131 282 104 621.6 0 104 283 129 621.6 0 129 284 136 621.6 0 136 285 128 621.6 0 128 286 80 621.6 0 80 287 95 621.6 0 95 288 116 621.6 0 116 289 77 621.6 0 77 290 165 621.6 0 165 291 121 621.6 0 121 292 266 621.6 0 266 293 186 621.6 0 186 294 389 621.6 0 389 295 1132 621.6 0 1132 296 1039 621.6 0 1039 297 1190 621.6 0 1190 298 340 621.6 0 340 299 65 621.6 0 65 300 88 621.6 0 88 301 64 621.6 0 64 Finished in 1.43 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 35,572 Reads with adapters: 4,725 (13.3%) Reads that were too short: 109 (0.3%) Reads written (passing filters): 4,616 (13.0%) Total basepairs processed: 7,315,271 bp Total written (filtered): 1,240,759 bp (17.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4725 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 38 0.0 3 0 31 5 2 17 3424 0.0 3 3144 211 51 18 18 14 0.0 3 2 8 2 2 19 28 0.0 3 2 9 11 6 20 876 0.0 3 811 50 7 8 21 4 0.0 3 0 3 0 1 22 2 0.0 3 0 0 2 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 30 12 0.0 3 0 0 0 12 33 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 1 43 110 0.0 3 0 0 2 108 45 1 0.0 3 0 0 0 1 46 15 0.0 3 0 0 0 15 47 1 0.0 3 0 0 1 48 3 0.0 3 0 0 0 3 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 1 51 4 0.0 3 0 0 0 4 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 1 63 9 0.0 3 0 0 0 9 64 5 0.0 3 0 0 1 4 66 3 0.0 3 0 0 0 3 69 6 0.0 3 0 0 0 6 70 3 0.0 3 0 0 0 3 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 4 0.0 3 0 0 0 4 94 3 0.0 3 0 0 0 3 95 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 103 7 0.0 3 0 0 0 7 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 18 0.0 3 0 0 0 18 114 8 0.0 3 0 0 1 7 116 5 0.0 3 0 0 0 5 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 131 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 4 0.0 3 0 0 0 4 138 2 0.0 3 0 0 0 2 144 5 0.0 3 0 0 0 5 145 12 0.0 3 0 0 1 11 152 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 165 4 0.0 3 0 0 0 4 167 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 3 0.0 3 0 0 0 3 233 1 0.0 3 0 0 0 1 234 2 0.0 3 0 0 0 2 235 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 4,616 Reads with adapters: 47 (1.0%) Reads written (passing filters): 4,616 (100.0%) Total basepairs processed: 1,240,759 bp Total written (filtered): 1,240,615 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 47 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.1% C: 17.0% G: 25.5% T: 6.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 44 72.1 0 44 4 3 18.0 0 3