This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.99 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 33,101 (1.3%) Reads that were too short: 8,574 (0.3%) Reads written (passing filters): 24,527 (1.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 5,293,742 bp (0.7%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 33101 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 952 621.6 0 952 7 3518 621.6 0 3518 8 173 621.6 0 173 9 3569 621.6 0 3569 10 39 621.6 0 39 11 30 621.6 0 30 12 47 621.6 0 47 13 28 621.6 0 28 14 27 621.6 0 27 15 18 621.6 0 18 16 35 621.6 0 35 17 34 621.6 0 34 18 45 621.6 0 45 19 28 621.6 0 28 20 27 621.6 0 27 21 27 621.6 0 27 22 29 621.6 0 29 23 35 621.6 0 35 24 28 621.6 0 28 25 48 621.6 0 48 26 22 621.6 0 22 27 37 621.6 0 37 28 43 621.6 0 43 29 39 621.6 0 39 30 40 621.6 0 40 31 34 621.6 0 34 32 36 621.6 0 36 33 33 621.6 0 33 34 25 621.6 0 25 35 33 621.6 0 33 36 38 621.6 0 38 37 37 621.6 0 37 38 52 621.6 0 52 39 40 621.6 0 40 40 54 621.6 0 54 41 32 621.6 0 32 42 33 621.6 0 33 43 49 621.6 0 49 44 125 621.6 0 125 45 70 621.6 0 70 46 142 621.6 0 142 47 44 621.6 0 44 48 33 621.6 0 33 49 31 621.6 0 31 50 31 621.6 0 31 51 49 621.6 0 49 52 54 621.6 0 54 53 44 621.6 0 44 54 42 621.6 0 42 55 26 621.6 0 26 56 36 621.6 0 36 57 49 621.6 0 49 58 65 621.6 0 65 59 59 621.6 0 59 60 47 621.6 0 47 61 44 621.6 0 44 62 41 621.6 0 41 63 28 621.6 0 28 64 37 621.6 0 37 65 40 621.6 0 40 66 35 621.6 0 35 67 45 621.6 0 45 68 35 621.6 0 35 69 42 621.6 0 42 70 33 621.6 0 33 71 26 621.6 0 26 72 19 621.6 0 19 73 55 621.6 0 55 74 41 621.6 0 41 75 45 621.6 0 45 76 39 621.6 0 39 77 43 621.6 0 43 78 34 621.6 0 34 79 45 621.6 0 45 80 45 621.6 0 45 81 48 621.6 0 48 82 50 621.6 0 50 83 53 621.6 0 53 84 55 621.6 0 55 85 49 621.6 0 49 86 46 621.6 0 46 87 47 621.6 0 47 88 51 621.6 0 51 89 34 621.6 0 34 90 45 621.6 0 45 91 62 621.6 0 62 92 56 621.6 0 56 93 57 621.6 0 57 94 52 621.6 0 52 95 51 621.6 0 51 96 61 621.6 0 61 97 54 621.6 0 54 98 50 621.6 0 50 99 47 621.6 0 47 100 42 621.6 0 42 101 44 621.6 0 44 102 48 621.6 0 48 103 70 621.6 0 70 104 71 621.6 0 71 105 69 621.6 0 69 106 318 621.6 0 318 107 163 621.6 0 163 108 270 621.6 0 270 109 44 621.6 0 44 110 47 621.6 0 47 111 49 621.6 0 49 112 55 621.6 0 55 113 53 621.6 0 53 114 211 621.6 0 211 115 70 621.6 0 70 116 142 621.6 0 142 117 49 621.6 0 49 118 46 621.6 0 46 119 50 621.6 0 50 120 54 621.6 0 54 121 58 621.6 0 58 122 55 621.6 0 55 123 54 621.6 0 54 124 46 621.6 0 46 125 48 621.6 0 48 126 62 621.6 0 62 127 77 621.6 0 77 128 637 621.6 0 637 129 281 621.6 0 281 130 274 621.6 0 274 131 80 621.6 0 80 132 68 621.6 0 68 133 74 621.6 0 74 134 86 621.6 0 86 135 118 621.6 0 118 136 247 621.6 0 247 137 185 621.6 0 185 138 268 621.6 0 268 139 56 621.6 0 56 140 43 621.6 0 43 141 130 621.6 0 130 142 65 621.6 0 65 143 141 621.6 0 141 144 100 621.6 0 100 145 65 621.6 0 65 146 68 621.6 0 68 147 87 621.6 0 87 148 1383 621.6 0 1383 149 958 621.6 0 958 150 1208 621.6 0 1208 151 48 621.6 0 48 152 48 621.6 0 48 153 51 621.6 0 51 154 50 621.6 0 50 155 176 621.6 0 176 156 128 621.6 0 128 157 142 621.6 0 142 158 192 621.6 0 192 159 41 621.6 0 41 160 30 621.6 0 30 161 70 621.6 0 70 162 79 621.6 0 79 163 193 621.6 0 193 164 99 621.6 0 99 165 135 621.6 0 135 166 60 621.6 0 60 167 49 621.6 0 49 168 52 621.6 0 52 169 40 621.6 0 40 170 34 621.6 0 34 171 37 621.6 0 37 172 33 621.6 0 33 173 35 621.6 0 35 174 39 621.6 0 39 175 43 621.6 0 43 176 37 621.6 0 37 177 47 621.6 0 47 178 40 621.6 0 40 179 53 621.6 0 53 180 110 621.6 0 110 181 138 621.6 0 138 182 119 621.6 0 119 183 117 621.6 0 117 184 44 621.6 0 44 185 56 621.6 0 56 186 53 621.6 0 53 187 56 621.6 0 56 188 25 621.6 0 25 189 45 621.6 0 45 190 59 621.6 0 59 191 52 621.6 0 52 192 59 621.6 0 59 193 80 621.6 0 80 194 33 621.6 0 33 195 42 621.6 0 42 196 55 621.6 0 55 197 44 621.6 0 44 198 54 621.6 0 54 199 42 621.6 0 42 200 35 621.6 0 35 201 40 621.6 0 40 202 87 621.6 0 87 203 54 621.6 0 54 204 116 621.6 0 116 205 36 621.6 0 36 206 33 621.6 0 33 207 50 621.6 0 50 208 37 621.6 0 37 209 57 621.6 0 57 210 38 621.6 0 38 211 41 621.6 0 41 212 53 621.6 0 53 213 73 621.6 0 73 214 76 621.6 0 76 215 105 621.6 0 105 216 69 621.6 0 69 217 57 621.6 0 57 218 41 621.6 0 41 219 37 621.6 0 37 220 43 621.6 0 43 221 35 621.6 0 35 222 53 621.6 0 53 223 70 621.6 0 70 224 51 621.6 0 51 225 48 621.6 0 48 226 63 621.6 0 63 227 27 621.6 0 27 228 55 621.6 0 55 229 34 621.6 0 34 230 43 621.6 0 43 231 39 621.6 0 39 232 66 621.6 0 66 233 51 621.6 0 51 234 83 621.6 0 83 235 74 621.6 0 74 236 49 621.6 0 49 237 49 621.6 0 49 238 80 621.6 0 80 239 64 621.6 0 64 240 36 621.6 0 36 241 50 621.6 0 50 242 51 621.6 0 51 243 56 621.6 0 56 244 67 621.6 0 67 245 41 621.6 0 41 246 47 621.6 0 47 247 73 621.6 0 73 248 75 621.6 0 75 249 44 621.6 0 44 250 53 621.6 0 53 251 57 621.6 0 57 252 63 621.6 0 63 253 83 621.6 0 83 254 75 621.6 0 75 255 63 621.6 0 63 256 62 621.6 0 62 257 57 621.6 0 57 258 42 621.6 0 42 259 51 621.6 0 51 260 60 621.6 0 60 261 357 621.6 0 357 262 186 621.6 0 186 263 223 621.6 0 223 264 58 621.6 0 58 265 59 621.6 0 59 266 56 621.6 0 56 267 54 621.6 0 54 268 55 621.6 0 55 269 66 621.6 0 66 270 88 621.6 0 88 271 552 621.6 0 552 272 236 621.6 0 236 273 244 621.6 0 244 274 117 621.6 0 117 275 72 621.6 0 72 276 67 621.6 0 67 277 57 621.6 0 57 278 78 621.6 0 78 279 92 621.6 0 92 280 89 621.6 0 89 281 75 621.6 0 75 282 64 621.6 0 64 283 59 621.6 0 59 284 80 621.6 0 80 285 76 621.6 0 76 286 53 621.6 0 53 287 47 621.6 0 47 288 69 621.6 0 69 289 52 621.6 0 52 290 101 621.6 0 101 291 75 621.6 0 75 292 103 621.6 0 103 293 93 621.6 0 93 294 140 621.6 0 140 295 330 621.6 0 330 296 316 621.6 0 316 297 385 621.6 0 385 298 139 621.6 0 139 299 50 621.6 0 50 300 51 621.6 0 51 301 67 621.6 0 67 Finished in 1.14 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 24,527 Reads with adapters: 3,785 (15.4%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 3,776 (15.4%) Total basepairs processed: 5,293,742 bp Total written (filtered): 1,026,093 bp (19.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3785 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 49 0.0 3 0 49 20 3688 0.0 4 3630 56 1 1 21 14 0.0 4 2 12 22 6 0.0 4 3 0 2 0 1 23 5 0.0 4 0 0 0 5 58 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 82 1 0.0 4 1 83 1 0.0 4 1 89 1 0.0 4 0 0 1 130 8 0.0 4 0 0 0 0 8 141 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 1 161 1 0.0 4 0 0 0 0 1 188 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 3,776 Reads with adapters: 862 (22.8%) Reads written (passing filters): 3,776 (100.0%) Total basepairs processed: 1,026,093 bp Total written (filtered): 1,018,058 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 862 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.6% G: 95.6% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 59.0 0 1 6 2 0.9 0 2 9 602 0.0 0 27 575 10 229 0.0 1 9 220 11 25 0.0 1 0 25 12 2 0.0 1 0 2 13 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.