This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.90 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 73,406 (2.9%) Reads that were too short: 27,271 (1.1%) Reads written (passing filters): 46,135 (1.8%) Total basepairs processed: 766,381,518 bp Total written (filtered): 11,920,579 bp (1.6%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 73406 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 809 621.6 0 809 7 15936 621.6 0 15936 8 700 621.6 0 700 9 13675 621.6 0 13675 10 45 621.6 0 45 11 43 621.6 0 43 12 44 621.6 0 44 13 33 621.6 0 33 14 55 621.6 0 55 15 44 621.6 0 44 16 51 621.6 0 51 17 34 621.6 0 34 18 45 621.6 0 45 19 38 621.6 0 38 20 37 621.6 0 37 21 42 621.6 0 42 22 53 621.6 0 53 23 38 621.6 0 38 24 49 621.6 0 49 25 87 621.6 0 87 26 56 621.6 0 56 27 52 621.6 0 52 28 54 621.6 0 54 29 52 621.6 0 52 30 41 621.6 0 41 31 42 621.6 0 42 32 63 621.6 0 63 33 51 621.6 0 51 34 47 621.6 0 47 35 41 621.6 0 41 36 72 621.6 0 72 37 46 621.6 0 46 38 45 621.6 0 45 39 27 621.6 0 27 40 48 621.6 0 48 41 51 621.6 0 51 42 61 621.6 0 61 43 44 621.6 0 44 44 66 621.6 0 66 45 50 621.6 0 50 46 46 621.6 0 46 47 46 621.6 0 46 48 65 621.6 0 65 49 55 621.6 0 55 50 42 621.6 0 42 51 62 621.6 0 62 52 42 621.6 0 42 53 55 621.6 0 55 54 54 621.6 0 54 55 58 621.6 0 58 56 50 621.6 0 50 57 56 621.6 0 56 58 45 621.6 0 45 59 60 621.6 0 60 60 66 621.6 0 66 61 86 621.6 0 86 62 62 621.6 0 62 63 77 621.6 0 77 64 58 621.6 0 58 65 72 621.6 0 72 66 72 621.6 0 72 67 1142 621.6 0 1142 68 231 621.6 0 231 69 224 621.6 0 224 70 34 621.6 0 34 71 51 621.6 0 51 72 60 621.6 0 60 73 55 621.6 0 55 74 65 621.6 0 65 75 78 621.6 0 78 76 61 621.6 0 61 77 36 621.6 0 36 78 70 621.6 0 70 79 49 621.6 0 49 80 52 621.6 0 52 81 59 621.6 0 59 82 42 621.6 0 42 83 46 621.6 0 46 84 47 621.6 0 47 85 51 621.6 0 51 86 75 621.6 0 75 87 42 621.6 0 42 88 46 621.6 0 46 89 65 621.6 0 65 90 47 621.6 0 47 91 43 621.6 0 43 92 50 621.6 0 50 93 60 621.6 0 60 94 92 621.6 0 92 95 51 621.6 0 51 96 46 621.6 0 46 97 59 621.6 0 59 98 74 621.6 0 74 99 46 621.6 0 46 100 68 621.6 0 68 101 34 621.6 0 34 102 77 621.6 0 77 103 39 621.6 0 39 104 47 621.6 0 47 105 51 621.6 0 51 106 62 621.6 0 62 107 50 621.6 0 50 108 41 621.6 0 41 109 52 621.6 0 52 110 59 621.6 0 59 111 44 621.6 0 44 112 47 621.6 0 47 113 69 621.6 0 69 114 49 621.6 0 49 115 37 621.6 0 37 116 61 621.6 0 61 117 45 621.6 0 45 118 48 621.6 0 48 119 61 621.6 0 61 120 90 621.6 0 90 121 52 621.6 0 52 122 60 621.6 0 60 123 71 621.6 0 71 124 45 621.6 0 45 125 40 621.6 0 40 126 57 621.6 0 57 127 63 621.6 0 63 128 62 621.6 0 62 129 79 621.6 0 79 130 66 621.6 0 66 131 62 621.6 0 62 132 149 621.6 0 149 133 152 621.6 0 152 134 323 621.6 0 323 135 53 621.6 0 53 136 33 621.6 0 33 137 47 621.6 0 47 138 67 621.6 0 67 139 63 621.6 0 63 140 60 621.6 0 60 141 46 621.6 0 46 142 39 621.6 0 39 143 63 621.6 0 63 144 52 621.6 0 52 145 70 621.6 0 70 146 90 621.6 0 90 147 70 621.6 0 70 148 69 621.6 0 69 149 55 621.6 0 55 150 74 621.6 0 74 151 90 621.6 0 90 152 602 621.6 0 602 153 523 621.6 0 523 154 542 621.6 0 542 155 80 621.6 0 80 156 74 621.6 0 74 157 53 621.6 0 53 158 105 621.6 0 105 159 54 621.6 0 54 160 60 621.6 0 60 161 76 621.6 0 76 162 51 621.6 0 51 163 102 621.6 0 102 164 64 621.6 0 64 165 111 621.6 0 111 166 68 621.6 0 68 167 49 621.6 0 49 168 57 621.6 0 57 169 43 621.6 0 43 170 52 621.6 0 52 171 54 621.6 0 54 172 40 621.6 0 40 173 60 621.6 0 60 174 78 621.6 0 78 175 59 621.6 0 59 176 43 621.6 0 43 177 75 621.6 0 75 178 58 621.6 0 58 179 56 621.6 0 56 180 87 621.6 0 87 181 71 621.6 0 71 182 81 621.6 0 81 183 120 621.6 0 120 184 179 621.6 0 179 185 181 621.6 0 181 186 115 621.6 0 115 187 44 621.6 0 44 188 39 621.6 0 39 189 79 621.6 0 79 190 56 621.6 0 56 191 73 621.6 0 73 192 132 621.6 0 132 193 94 621.6 0 94 194 127 621.6 0 127 195 62 621.6 0 62 196 49 621.6 0 49 197 56 621.6 0 56 198 77 621.6 0 77 199 125 621.6 0 125 200 101 621.6 0 101 201 100 621.6 0 100 202 116 621.6 0 116 203 53 621.6 0 53 204 56 621.6 0 56 205 105 621.6 0 105 206 97 621.6 0 97 207 104 621.6 0 104 208 62 621.6 0 62 209 110 621.6 0 110 210 69 621.6 0 69 211 72 621.6 0 72 212 108 621.6 0 108 213 76 621.6 0 76 214 109 621.6 0 109 215 158 621.6 0 158 216 161 621.6 0 161 217 374 621.6 0 374 218 607 621.6 0 607 219 9325 621.6 0 9325 220 3699 621.6 0 3699 221 4603 621.6 0 4603 222 164 621.6 0 164 223 64 621.6 0 64 224 79 621.6 0 79 225 64 621.6 0 64 226 52 621.6 0 52 227 63 621.6 0 63 228 55 621.6 0 55 229 66 621.6 0 66 230 53 621.6 0 53 231 51 621.6 0 51 232 67 621.6 0 67 233 40 621.6 0 40 234 67 621.6 0 67 235 75 621.6 0 75 236 59 621.6 0 59 237 77 621.6 0 77 238 59 621.6 0 59 239 73 621.6 0 73 240 60 621.6 0 60 241 78 621.6 0 78 242 71 621.6 0 71 243 69 621.6 0 69 244 81 621.6 0 81 245 49 621.6 0 49 246 59 621.6 0 59 247 70 621.6 0 70 248 156 621.6 0 156 249 212 621.6 0 212 250 245 621.6 0 245 251 109 621.6 0 109 252 102 621.6 0 102 253 94 621.6 0 94 254 97 621.6 0 97 255 84 621.6 0 84 256 76 621.6 0 76 257 59 621.6 0 59 258 62 621.6 0 62 259 70 621.6 0 70 260 60 621.6 0 60 261 66 621.6 0 66 262 65 621.6 0 65 263 58 621.6 0 58 264 53 621.6 0 53 265 58 621.6 0 58 266 93 621.6 0 93 267 78 621.6 0 78 268 80 621.6 0 80 269 67 621.6 0 67 270 75 621.6 0 75 271 72 621.6 0 72 272 97 621.6 0 97 273 99 621.6 0 99 274 91 621.6 0 91 275 70 621.6 0 70 276 64 621.6 0 64 277 77 621.6 0 77 278 115 621.6 0 115 279 293 621.6 0 293 280 292 621.6 0 292 281 298 621.6 0 298 282 98 621.6 0 98 283 85 621.6 0 85 284 91 621.6 0 91 285 64 621.6 0 64 286 62 621.6 0 62 287 97 621.6 0 97 288 63 621.6 0 63 289 78 621.6 0 78 290 64 621.6 0 64 291 72 621.6 0 72 292 116 621.6 0 116 293 104 621.6 0 104 294 110 621.6 0 110 295 64 621.6 0 64 296 65 621.6 0 65 297 89 621.6 0 89 298 86 621.6 0 86 299 91 621.6 0 91 300 127 621.6 0 127 301 129 621.6 0 129 Finished in 2.34 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 46,135 Reads with adapters: 16,497 (35.8%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 16,488 (35.7%) Total basepairs processed: 11,920,579 bp Total written (filtered): 4,518,619 bp (37.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16497 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 8 19 230 0.0 3 0 224 5 0 1 20 16219 0.0 4 15938 272 8 0 1 21 23 0.0 4 3 20 22 3 0.0 4 1 0 2 23 4 0.0 4 0 0 0 4 24 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 270 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 16,488 Reads with adapters: 528 (3.2%) Reads written (passing filters): 16,488 (100.0%) Total basepairs processed: 4,518,619 bp Total written (filtered): 4,512,461 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 528 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 5.5% G: 87.3% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 31 257.6 0 31 4 2 64.4 0 2 5 2 16.1 0 2 9 2 0.1 0 0 2 10 9 0.0 1 0 9 11 440 0.0 1 4 436 12 39 0.0 1 1 38 210 2 0.0 1 0 2 211 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.