This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.44 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 297,845 (11.7%) Reads that were too short: 35,907 (1.4%) Reads written (passing filters): 261,938 (10.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 56,552,691 bp (7.4%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 297845 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 218 621.6 0 218 7 531 621.6 0 531 8 4766 621.6 0 4766 9 7552 621.6 0 7552 10 30784 621.6 0 30784 11 64 621.6 0 64 12 134 621.6 0 134 13 27 621.6 0 27 14 19 621.6 0 19 15 31 621.6 0 31 16 33 621.6 0 33 17 39 621.6 0 39 18 18 621.6 0 18 19 50 621.6 0 50 20 24 621.6 0 24 21 33 621.6 0 33 22 30 621.6 0 30 23 45 621.6 0 45 24 75 621.6 0 75 25 45 621.6 0 45 26 57 621.6 0 57 27 54 621.6 0 54 28 42 621.6 0 42 29 17 621.6 0 17 30 48 621.6 0 48 31 58 621.6 0 58 32 50 621.6 0 50 33 31 621.6 0 31 34 23 621.6 0 23 35 38 621.6 0 38 36 41 621.6 0 41 37 33 621.6 0 33 38 81 621.6 0 81 39 153 621.6 0 153 40 223 621.6 0 223 41 301 621.6 0 301 42 44 621.6 0 44 43 45 621.6 0 45 44 41 621.6 0 41 45 26 621.6 0 26 46 40 621.6 0 40 47 48 621.6 0 48 48 83 621.6 0 83 49 80 621.6 0 80 50 77 621.6 0 77 51 147 621.6 0 147 52 390 621.6 0 390 53 244 621.6 0 244 54 209 621.6 0 209 55 26 621.6 0 26 56 42 621.6 0 42 57 58 621.6 0 58 58 45 621.6 0 45 59 36 621.6 0 36 60 70 621.6 0 70 61 32 621.6 0 32 62 45 621.6 0 45 63 53 621.6 0 53 64 38 621.6 0 38 65 49 621.6 0 49 66 75 621.6 0 75 67 82 621.6 0 82 68 126 621.6 0 126 69 72 621.6 0 72 70 121 621.6 0 121 71 269 621.6 0 269 72 455 621.6 0 455 73 240 621.6 0 240 74 125 621.6 0 125 75 136 621.6 0 136 76 199 621.6 0 199 77 274 621.6 0 274 78 542 621.6 0 542 79 994 621.6 0 994 80 249 621.6 0 249 81 412 621.6 0 412 82 578 621.6 0 578 83 328 621.6 0 328 84 400 621.6 0 400 85 683 621.6 0 683 86 600 621.6 0 600 87 849 621.6 0 849 88 799 621.6 0 799 89 1273 621.6 0 1273 90 2125 621.6 0 2125 91 3133 621.6 0 3133 92 56372 621.6 0 56372 93 46589 621.6 0 46589 94 75498 621.6 0 75498 95 871 621.6 0 871 96 43 621.6 0 43 97 55 621.6 0 55 98 69 621.6 0 69 99 58 621.6 0 58 100 26 621.6 0 26 101 16 621.6 0 16 102 39 621.6 0 39 103 29 621.6 0 29 104 20 621.6 0 20 105 25 621.6 0 25 106 20 621.6 0 20 107 31 621.6 0 31 108 66 621.6 0 66 109 70 621.6 0 70 110 47 621.6 0 47 111 48 621.6 0 48 112 85 621.6 0 85 113 25 621.6 0 25 114 62 621.6 0 62 115 59 621.6 0 59 116 60 621.6 0 60 117 60 621.6 0 60 118 50 621.6 0 50 119 30 621.6 0 30 120 20 621.6 0 20 121 17 621.6 0 17 122 14 621.6 0 14 123 25 621.6 0 25 124 18 621.6 0 18 125 30 621.6 0 30 126 28 621.6 0 28 127 14 621.6 0 14 128 25 621.6 0 25 129 45 621.6 0 45 130 36 621.6 0 36 131 42 621.6 0 42 132 34 621.6 0 34 133 30 621.6 0 30 134 30 621.6 0 30 135 35 621.6 0 35 136 52 621.6 0 52 137 45 621.6 0 45 138 53 621.6 0 53 139 40 621.6 0 40 140 37 621.6 0 37 141 17 621.6 0 17 142 27 621.6 0 27 143 45 621.6 0 45 144 49 621.6 0 49 145 43 621.6 0 43 146 31 621.6 0 31 147 23 621.6 0 23 148 24 621.6 0 24 149 32 621.6 0 32 150 20 621.6 0 20 151 30 621.6 0 30 152 34 621.6 0 34 153 25 621.6 0 25 154 33 621.6 0 33 155 26 621.6 0 26 156 23 621.6 0 23 157 39 621.6 0 39 158 33 621.6 0 33 159 44 621.6 0 44 160 34 621.6 0 34 161 29 621.6 0 29 162 22 621.6 0 22 163 34 621.6 0 34 164 25 621.6 0 25 165 34 621.6 0 34 166 29 621.6 0 29 167 23 621.6 0 23 168 14 621.6 0 14 169 23 621.6 0 23 170 26 621.6 0 26 171 43 621.6 0 43 172 27 621.6 0 27 173 41 621.6 0 41 174 33 621.6 0 33 175 36 621.6 0 36 176 37 621.6 0 37 177 123 621.6 0 123 178 287 621.6 0 287 179 139 621.6 0 139 180 230 621.6 0 230 181 43 621.6 0 43 182 38 621.6 0 38 183 55 621.6 0 55 184 61 621.6 0 61 185 63 621.6 0 63 186 99 621.6 0 99 187 162 621.6 0 162 188 267 621.6 0 267 189 124 621.6 0 124 190 130 621.6 0 130 191 308 621.6 0 308 192 174 621.6 0 174 193 83 621.6 0 83 194 103 621.6 0 103 195 139 621.6 0 139 196 208 621.6 0 208 197 191 621.6 0 191 198 228 621.6 0 228 199 384 621.6 0 384 200 713 621.6 0 713 201 12510 621.6 0 12510 202 6041 621.6 0 6041 203 12970 621.6 0 12970 204 234 621.6 0 234 205 489 621.6 0 489 206 750 621.6 0 750 207 717 621.6 0 717 208 354 621.6 0 354 209 167 621.6 0 167 210 90 621.6 0 90 211 170 621.6 0 170 212 87 621.6 0 87 213 96 621.6 0 96 214 70 621.6 0 70 215 111 621.6 0 111 216 133 621.6 0 133 217 158 621.6 0 158 218 86 621.6 0 86 219 40 621.6 0 40 220 80 621.6 0 80 221 168 621.6 0 168 222 90 621.6 0 90 223 213 621.6 0 213 224 100 621.6 0 100 225 346 621.6 0 346 226 1311 621.6 0 1311 227 1149 621.6 0 1149 228 745 621.6 0 745 229 214 621.6 0 214 230 30 621.6 0 30 231 24 621.6 0 24 232 27 621.6 0 27 233 25 621.6 0 25 234 23 621.6 0 23 235 28 621.6 0 28 236 74 621.6 0 74 237 69 621.6 0 69 238 109 621.6 0 109 239 17 621.6 0 17 240 23 621.6 0 23 241 20 621.6 0 20 242 23 621.6 0 23 243 41 621.6 0 41 244 35 621.6 0 35 245 39 621.6 0 39 246 56 621.6 0 56 247 56 621.6 0 56 248 59 621.6 0 59 249 62 621.6 0 62 250 44 621.6 0 44 251 47 621.6 0 47 252 34 621.6 0 34 253 29 621.6 0 29 254 31 621.6 0 31 255 44 621.6 0 44 256 33 621.6 0 33 257 54 621.6 0 54 258 84 621.6 0 84 259 403 621.6 0 403 260 678 621.6 0 678 261 907 621.6 0 907 262 461 621.6 0 461 263 111 621.6 0 111 264 61 621.6 0 61 265 78 621.6 0 78 266 39 621.6 0 39 267 32 621.6 0 32 268 36 621.6 0 36 269 38 621.6 0 38 270 174 621.6 0 174 271 263 621.6 0 263 272 199 621.6 0 199 273 81 621.6 0 81 274 42 621.6 0 42 275 69 621.6 0 69 276 38 621.6 0 38 277 55 621.6 0 55 278 44 621.6 0 44 279 56 621.6 0 56 280 162 621.6 0 162 281 152 621.6 0 152 282 177 621.6 0 177 283 174 621.6 0 174 284 195 621.6 0 195 285 180 621.6 0 180 286 208 621.6 0 208 287 495 621.6 0 495 288 42 621.6 0 42 289 33 621.6 0 33 290 25 621.6 0 25 291 43 621.6 0 43 292 41 621.6 0 41 293 61 621.6 0 61 294 42 621.6 0 42 295 62 621.6 0 62 296 175 621.6 0 175 297 682 621.6 0 682 298 252 621.6 0 252 299 373 621.6 0 373 300 37 621.6 0 37 301 12 621.6 0 12 Finished in 9.69 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 261,938 Reads with adapters: 8,105 (3.1%) Reads that were too short: 572 (0.2%) Reads written (passing filters): 7,533 (2.9%) Total basepairs processed: 56,552,691 bp Total written (filtered): 1,432,006 bp (2.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8105 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 0 3 16 43 0.0 3 0 32 4 7 17 3517 0.0 3 3221 249 38 9 18 21 0.0 3 1 13 5 2 19 20 0.0 3 0 0 10 10 20 14 0.0 3 2 0 0 12 25 4 0.0 3 0 0 1 3 26 1 0.0 3 0 0 0 1 28 3 0.0 3 0 0 0 3 30 15 0.0 3 0 0 1 14 31 5 0.0 3 0 0 0 5 32 1 0.0 3 0 0 0 1 34 3 0.0 3 0 0 0 3 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 1 61 3 0.0 3 0 0 0 3 66 4 0.0 3 1 0 0 3 67 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 43 0.0 3 0 0 2 41 75 14 0.0 3 0 0 0 14 76 7 0.0 3 0 0 0 7 77 13 0.0 3 0 0 0 13 78 4 0.0 3 0 0 0 4 79 123 0.0 3 0 0 0 123 80 7 0.0 3 0 0 1 6 81 1 0.0 3 0 0 0 1 82 18 0.0 3 0 0 0 18 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 98 16 0.0 3 0 0 0 16 99 2588 0.0 3 0 0 22 2566 100 666 0.0 3 1 0 7 658 101 11 0.0 3 0 0 2 9 102 216 0.0 3 0 0 10 206 103 116 0.0 3 0 0 3 113 105 7 0.0 3 0 0 0 7 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 1 1 122 3 0.0 3 0 0 0 3 123 9 0.0 3 0 0 0 9 124 6 0.0 3 0 0 0 6 125 14 0.0 3 0 0 1 13 126 7 0.0 3 0 0 0 7 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 4 0.0 3 0 0 0 4 135 14 0.0 3 0 0 1 13 136 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 144 8 0.0 3 5 1 2 145 3 0.0 3 1 0 1 1 146 4 0.0 3 0 0 0 4 147 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 47 0.0 3 0 0 2 45 151 21 0.0 3 0 0 1 20 152 7 0.0 3 0 0 0 7 153 16 0.0 3 0 0 1 15 156 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 159 14 0.0 3 0 0 0 14 160 28 0.0 3 0 0 0 28 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 174 2 0.0 3 0 0 0 2 175 2 0.0 3 0 0 0 2 176 5 0.0 3 0 0 1 4 177 2 0.0 3 0 0 0 2 178 3 0.0 3 0 0 0 3 180 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 189 3 0.0 3 0 0 0 3 190 5 0.0 3 0 0 0 5 191 18 0.0 3 0 0 0 18 192 3 0.0 3 0 0 0 3 195 4 0.0 3 0 0 0 4 196 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 199 8 0.0 3 0 0 0 8 200 147 0.0 3 0 0 2 145 201 53 0.0 3 0 0 2 51 202 12 0.0 3 0 0 0 12 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 12 0.0 3 0 0 0 12 210 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 234 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 276 4 0.0 3 0 0 0 4 277 2 0.0 3 0 0 0 2 278 7 0.0 3 0 0 0 7 279 5 0.0 3 0 0 0 5 280 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 285 2 0.0 3 0 0 0 2 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (29 us/read; 2.05 M reads/minute). === Summary === Total reads processed: 7,533 Reads with adapters: 18 (0.2%) Reads written (passing filters): 7,533 (100.0%) Total basepairs processed: 1,432,006 bp Total written (filtered): 1,431,940 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 18 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.2% C: 0.0% G: 66.7% T: 11.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 13 117.7 0 13 4 3 29.4 0 3 5 1 7.4 0 1 10 1 0.0 1 0 1