This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.78 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 327,082 (12.8%) Reads that were too short: 14,371 (0.6%) Reads written (passing filters): 312,711 (12.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 77,222,561 bp (10.1%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 327082 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1774 621.6 0 1774 7 69015 621.6 0 69015 8 50304 621.6 0 50304 9 94 621.6 0 94 10 133 621.6 0 133 11 23 621.6 0 23 12 19 621.6 0 19 13 46 621.6 0 46 14 7 621.6 0 7 15 11 621.6 0 11 16 5 621.6 0 5 17 9 621.6 0 9 18 6 621.6 0 6 19 14 621.6 0 14 20 13 621.6 0 13 21 37 621.6 0 37 22 17 621.6 0 17 23 10 621.6 0 10 24 13 621.6 0 13 25 15 621.6 0 15 26 7 621.6 0 7 27 19 621.6 0 19 28 7 621.6 0 7 29 13 621.6 0 13 30 23 621.6 0 23 31 6 621.6 0 6 32 7 621.6 0 7 33 12 621.6 0 12 34 15 621.6 0 15 35 25 621.6 0 25 36 16 621.6 0 16 37 9 621.6 0 9 38 8 621.6 0 8 39 20 621.6 0 20 40 22 621.6 0 22 41 29 621.6 0 29 42 84 621.6 0 84 43 48 621.6 0 48 44 46 621.6 0 46 45 64 621.6 0 64 46 48 621.6 0 48 47 40 621.6 0 40 48 20 621.6 0 20 49 87 621.6 0 87 50 39 621.6 0 39 51 63 621.6 0 63 52 456 621.6 0 456 53 76 621.6 0 76 54 39 621.6 0 39 55 102 621.6 0 102 56 24 621.6 0 24 57 18 621.6 0 18 58 19 621.6 0 19 59 72 621.6 0 72 60 61 621.6 0 61 61 263 621.6 0 263 62 681 621.6 0 681 63 135 621.6 0 135 64 258 621.6 0 258 65 586 621.6 0 586 66 598 621.6 0 598 67 2261 621.6 0 2261 68 261 621.6 0 261 69 211 621.6 0 211 70 69 621.6 0 69 71 140 621.6 0 140 72 348 621.6 0 348 73 377 621.6 0 377 74 694 621.6 0 694 75 1014 621.6 0 1014 76 1027 621.6 0 1027 77 2683 621.6 0 2683 78 6842 621.6 0 6842 79 68790 621.6 0 68790 80 30118 621.6 0 30118 81 57240 621.6 0 57240 82 419 621.6 0 419 83 236 621.6 0 236 84 43 621.6 0 43 85 26 621.6 0 26 86 45 621.6 0 45 87 46 621.6 0 46 88 26 621.6 0 26 89 37 621.6 0 37 90 52 621.6 0 52 91 11 621.6 0 11 92 22 621.6 0 22 93 21 621.6 0 21 94 16 621.6 0 16 95 28 621.6 0 28 96 27 621.6 0 27 97 11 621.6 0 11 98 13 621.6 0 13 99 20 621.6 0 20 100 14 621.6 0 14 101 27 621.6 0 27 102 17 621.6 0 17 103 33 621.6 0 33 104 23 621.6 0 23 105 13 621.6 0 13 106 23 621.6 0 23 107 10 621.6 0 10 108 22 621.6 0 22 109 26 621.6 0 26 110 40 621.6 0 40 111 30 621.6 0 30 112 32 621.6 0 32 113 41 621.6 0 41 114 46 621.6 0 46 115 50 621.6 0 50 116 292 621.6 0 292 117 170 621.6 0 170 118 528 621.6 0 528 119 70 621.6 0 70 120 81 621.6 0 81 121 222 621.6 0 222 122 147 621.6 0 147 123 842 621.6 0 842 124 742 621.6 0 742 125 601 621.6 0 601 126 146 621.6 0 146 127 63 621.6 0 63 128 53 621.6 0 53 129 84 621.6 0 84 130 470 621.6 0 470 131 400 621.6 0 400 132 593 621.6 0 593 133 60 621.6 0 60 134 32 621.6 0 32 135 54 621.6 0 54 136 49 621.6 0 49 137 73 621.6 0 73 138 50 621.6 0 50 139 62 621.6 0 62 140 104 621.6 0 104 141 122 621.6 0 122 142 192 621.6 0 192 143 73 621.6 0 73 144 54 621.6 0 54 145 113 621.6 0 113 146 92 621.6 0 92 147 162 621.6 0 162 148 107 621.6 0 107 149 117 621.6 0 117 150 649 621.6 0 649 151 642 621.6 0 642 152 1952 621.6 0 1952 153 84 621.6 0 84 154 23 621.6 0 23 155 48 621.6 0 48 156 41 621.6 0 41 157 48 621.6 0 48 158 70 621.6 0 70 159 34 621.6 0 34 160 40 621.6 0 40 161 31 621.6 0 31 162 49 621.6 0 49 163 50 621.6 0 50 164 38 621.6 0 38 165 72 621.6 0 72 166 87 621.6 0 87 167 125 621.6 0 125 168 96 621.6 0 96 169 90 621.6 0 90 170 99 621.6 0 99 171 86 621.6 0 86 172 123 621.6 0 123 173 106 621.6 0 106 174 182 621.6 0 182 175 33 621.6 0 33 176 34 621.6 0 34 177 36 621.6 0 36 178 44 621.6 0 44 179 37 621.6 0 37 180 45 621.6 0 45 181 38 621.6 0 38 182 66 621.6 0 66 183 51 621.6 0 51 184 50 621.6 0 50 185 44 621.6 0 44 186 123 621.6 0 123 187 80 621.6 0 80 188 72 621.6 0 72 189 41 621.6 0 41 190 41 621.6 0 41 191 41 621.6 0 41 192 58 621.6 0 58 193 71 621.6 0 71 194 56 621.6 0 56 195 48 621.6 0 48 196 47 621.6 0 47 197 60 621.6 0 60 198 82 621.6 0 82 199 55 621.6 0 55 200 78 621.6 0 78 201 44 621.6 0 44 202 36 621.6 0 36 203 49 621.6 0 49 204 37 621.6 0 37 205 61 621.6 0 61 206 44 621.6 0 44 207 49 621.6 0 49 208 55 621.6 0 55 209 55 621.6 0 55 210 103 621.6 0 103 211 65 621.6 0 65 212 74 621.6 0 74 213 77 621.6 0 77 214 79 621.6 0 79 215 66 621.6 0 66 216 86 621.6 0 86 217 171 621.6 0 171 218 299 621.6 0 299 219 2088 621.6 0 2088 220 132 621.6 0 132 221 108 621.6 0 108 222 112 621.6 0 112 223 126 621.6 0 126 224 135 621.6 0 135 225 140 621.6 0 140 226 710 621.6 0 710 227 601 621.6 0 601 228 973 621.6 0 973 229 150 621.6 0 150 230 113 621.6 0 113 231 82 621.6 0 82 232 88 621.6 0 88 233 89 621.6 0 89 234 70 621.6 0 70 235 37 621.6 0 37 236 55 621.6 0 55 237 69 621.6 0 69 238 59 621.6 0 59 239 69 621.6 0 69 240 77 621.6 0 77 241 74 621.6 0 74 242 64 621.6 0 64 243 71 621.6 0 71 244 54 621.6 0 54 245 88 621.6 0 88 246 174 621.6 0 174 247 187 621.6 0 187 248 180 621.6 0 180 249 100 621.6 0 100 250 147 621.6 0 147 251 120 621.6 0 120 252 105 621.6 0 105 253 119 621.6 0 119 254 60 621.6 0 60 255 101 621.6 0 101 256 99 621.6 0 99 257 69 621.6 0 69 258 57 621.6 0 57 259 108 621.6 0 108 260 103 621.6 0 103 261 131 621.6 0 131 262 86 621.6 0 86 263 89 621.6 0 89 264 93 621.6 0 93 265 92 621.6 0 92 266 160 621.6 0 160 267 99 621.6 0 99 268 101 621.6 0 101 269 74 621.6 0 74 270 65 621.6 0 65 271 62 621.6 0 62 272 83 621.6 0 83 273 92 621.6 0 92 274 96 621.6 0 96 275 100 621.6 0 100 276 121 621.6 0 121 277 178 621.6 0 178 278 104 621.6 0 104 279 159 621.6 0 159 280 164 621.6 0 164 281 193 621.6 0 193 282 80 621.6 0 80 283 62 621.6 0 62 284 77 621.6 0 77 285 85 621.6 0 85 286 106 621.6 0 106 287 132 621.6 0 132 288 110 621.6 0 110 289 85 621.6 0 85 290 124 621.6 0 124 291 135 621.6 0 135 292 188 621.6 0 188 293 127 621.6 0 127 294 114 621.6 0 114 295 88 621.6 0 88 296 85 621.6 0 85 297 125 621.6 0 125 298 164 621.6 0 164 299 202 621.6 0 202 300 242 621.6 0 242 301 129 621.6 0 129 Finished in 12.81 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 312,711 Reads with adapters: 58,912 (18.8%) Reads that were too short: 395 (0.1%) Reads written (passing filters): 58,517 (18.7%) Total basepairs processed: 77,222,561 bp Total written (filtered): 16,146,375 bp (20.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58912 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 32 0.0 3 0 0 21 11 16 555 0.0 3 0 456 65 34 17 57116 0.0 3 52413 3800 714 189 18 133 0.0 3 4 106 14 9 19 219 0.0 3 18 5 118 78 20 45 0.0 3 1 2 0 42 22 1 0.0 3 0 0 0 1 23 6 0.0 3 0 0 0 6 26 10 0.0 3 0 0 0 10 28 3 0.0 3 0 0 0 3 30 1 0.0 3 1 31 4 0.0 3 0 0 0 4 35 2 0.0 3 1 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 1 41 102 0.0 3 0 0 1 101 43 1 0.0 3 0 0 0 1 44 20 0.0 3 0 0 3 17 45 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 10 0.0 3 0 0 1 9 49 3 0.0 3 0 0 0 3 50 5 0.0 3 0 0 0 5 51 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 1 2 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 9 0.0 3 0 0 0 9 56 1 0.0 3 0 0 0 1 57 6 0.0 3 0 0 0 6 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 1 1 61 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 4 0.0 3 0 0 0 4 70 8 0.0 3 0 0 0 8 73 7 0.0 3 0 0 0 7 74 2 0.0 3 0 0 0 2 76 16 0.0 3 0 0 2 14 78 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 80 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 10 0.0 3 0 0 0 10 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 1 0.0 3 0 1 92 6 0.0 3 0 0 0 6 93 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 1 1 99 2 0.0 3 0 0 1 1 101 4 0.0 3 0 0 0 4 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 61 0.0 3 0 0 2 59 107 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 1 1 109 25 0.0 3 0 0 2 23 110 5 0.0 3 0 0 0 5 111 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 125 6 0.0 3 0 0 1 5 126 2 0.0 3 0 0 1 1 127 4 0.0 3 0 0 0 4 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 4 0.0 3 0 0 0 4 133 22 0.0 3 0 0 1 21 134 7 0.0 3 0 0 0 7 135 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 5 0.0 3 0 0 0 5 143 4 0.0 3 0 0 0 4 144 5 0.0 3 0 0 0 5 145 2 0.0 3 0 0 0 2 146 12 0.0 3 0 0 0 12 147 1 0.0 3 0 0 0 1 149 18 0.0 3 0 0 0 18 150 5 0.0 3 0 0 0 5 151 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 157 5 0.0 3 0 0 0 5 158 3 0.0 3 0 0 0 3 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 5 0.0 3 0 0 1 4 164 1 0.0 3 0 0 1 165 5 0.0 3 0 0 1 4 166 7 0.0 3 0 0 0 7 167 3 0.0 3 0 0 1 2 168 1 0.0 3 0 0 0 1 170 4 0.0 3 0 0 0 4 171 3 0.0 3 0 0 0 3 172 3 0.0 3 0 0 0 3 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 187 3 0.0 3 0 0 0 3 189 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 1 205 1 0.0 3 0 0 0 1 207 29 0.0 3 0 0 0 29 208 24 0.0 3 0 0 1 23 209 52 0.0 3 0 0 3 49 210 42 0.0 3 0 0 0 42 211 2 0.0 3 0 0 0 2 213 2 0.0 3 0 0 0 2 215 2 0.0 3 0 0 1 1 216 5 0.0 3 0 0 1 4 217 5 0.0 3 0 0 0 5 218 3 0.0 3 0 0 0 3 219 2 0.0 3 0 0 0 2 220 2 0.0 3 0 0 0 2 221 3 0.0 3 0 0 0 3 222 2 0.0 3 0 0 1 1 223 2 0.0 3 0 0 0 2 226 4 0.0 3 0 0 0 4 227 1 0.0 3 0 0 0 1 228 2 0.0 3 0 0 0 2 234 1 0.0 3 0 0 0 1 236 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 10 0.0 3 0 0 0 10 282 3 0.0 3 0 0 0 3 283 4 0.0 3 0 0 0 4 284 2 0.0 3 0 0 0 2 288 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.06 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 58,517 Reads with adapters: 198 (0.3%) Reads written (passing filters): 58,517 (100.0%) Total basepairs processed: 16,146,375 bp Total written (filtered): 16,145,773 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 198 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.1% C: 62.1% G: 21.7% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 192 914.3 0 192 4 5 228.6 0 5 6 1 14.3 0 1