This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.88 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 391,591 (15.4%) Reads that were too short: 8,515 (0.3%) Reads written (passing filters): 383,076 (15.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 93,585,323 bp (12.2%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 391591 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 961 621.6 0 961 7 65858 621.6 0 65858 8 61014 621.6 0 61014 9 54 621.6 0 54 10 33 621.6 0 33 11 15 621.6 0 15 12 23 621.6 0 23 13 14 621.6 0 14 14 5 621.6 0 5 15 2 621.6 0 2 16 2 621.6 0 2 17 6 621.6 0 6 18 8 621.6 0 8 19 23 621.6 0 23 20 13 621.6 0 13 21 27 621.6 0 27 22 31 621.6 0 31 23 17 621.6 0 17 24 10 621.6 0 10 25 11 621.6 0 11 26 14 621.6 0 14 27 22 621.6 0 22 28 9 621.6 0 9 29 22 621.6 0 22 30 26 621.6 0 26 31 9 621.6 0 9 32 18 621.6 0 18 33 16 621.6 0 16 34 17 621.6 0 17 35 42 621.6 0 42 36 29 621.6 0 29 37 9 621.6 0 9 38 5 621.6 0 5 39 34 621.6 0 34 40 41 621.6 0 41 41 22 621.6 0 22 42 98 621.6 0 98 43 69 621.6 0 69 44 59 621.6 0 59 45 96 621.6 0 96 46 81 621.6 0 81 47 52 621.6 0 52 48 35 621.6 0 35 49 157 621.6 0 157 50 42 621.6 0 42 51 67 621.6 0 67 52 718 621.6 0 718 53 59 621.6 0 59 54 29 621.6 0 29 55 80 621.6 0 80 56 29 621.6 0 29 57 19 621.6 0 19 58 20 621.6 0 20 59 99 621.6 0 99 60 74 621.6 0 74 61 318 621.6 0 318 62 899 621.6 0 899 63 120 621.6 0 120 64 187 621.6 0 187 65 240 621.6 0 240 66 344 621.6 0 344 67 1136 621.6 0 1136 68 346 621.6 0 346 69 386 621.6 0 386 70 111 621.6 0 111 71 182 621.6 0 182 72 347 621.6 0 347 73 464 621.6 0 464 74 735 621.6 0 735 75 1276 621.6 0 1276 76 1337 621.6 0 1337 77 3475 621.6 0 3475 78 8879 621.6 0 8879 79 98665 621.6 0 98665 80 41626 621.6 0 41626 81 82032 621.6 0 82032 82 455 621.6 0 455 83 128 621.6 0 128 84 29 621.6 0 29 85 29 621.6 0 29 86 19 621.6 0 19 87 24 621.6 0 24 88 18 621.6 0 18 89 17 621.6 0 17 90 12 621.6 0 12 91 19 621.6 0 19 92 11 621.6 0 11 93 14 621.6 0 14 94 24 621.6 0 24 95 12 621.6 0 12 96 14 621.6 0 14 97 15 621.6 0 15 98 13 621.6 0 13 99 14 621.6 0 14 100 29 621.6 0 29 101 16 621.6 0 16 102 21 621.6 0 21 103 13 621.6 0 13 104 16 621.6 0 16 105 23 621.6 0 23 106 22 621.6 0 22 107 17 621.6 0 17 108 23 621.6 0 23 109 18 621.6 0 18 110 33 621.6 0 33 111 13 621.6 0 13 112 22 621.6 0 22 113 30 621.6 0 30 114 22 621.6 0 22 115 24 621.6 0 24 116 112 621.6 0 112 117 77 621.6 0 77 118 151 621.6 0 151 119 61 621.6 0 61 120 39 621.6 0 39 121 84 621.6 0 84 122 76 621.6 0 76 123 695 621.6 0 695 124 664 621.6 0 664 125 547 621.6 0 547 126 39 621.6 0 39 127 31 621.6 0 31 128 40 621.6 0 40 129 33 621.6 0 33 130 135 621.6 0 135 131 134 621.6 0 134 132 218 621.6 0 218 133 36 621.6 0 36 134 28 621.6 0 28 135 30 621.6 0 30 136 38 621.6 0 38 137 45 621.6 0 45 138 28 621.6 0 28 139 34 621.6 0 34 140 59 621.6 0 59 141 85 621.6 0 85 142 211 621.6 0 211 143 43 621.6 0 43 144 42 621.6 0 42 145 48 621.6 0 48 146 39 621.6 0 39 147 97 621.6 0 97 148 52 621.6 0 52 149 53 621.6 0 53 150 506 621.6 0 506 151 483 621.6 0 483 152 1582 621.6 0 1582 153 37 621.6 0 37 154 24 621.6 0 24 155 41 621.6 0 41 156 32 621.6 0 32 157 28 621.6 0 28 158 53 621.6 0 53 159 29 621.6 0 29 160 30 621.6 0 30 161 26 621.6 0 26 162 26 621.6 0 26 163 30 621.6 0 30 164 25 621.6 0 25 165 23 621.6 0 23 166 26 621.6 0 26 167 59 621.6 0 59 168 35 621.6 0 35 169 33 621.6 0 33 170 69 621.6 0 69 171 59 621.6 0 59 172 72 621.6 0 72 173 46 621.6 0 46 174 106 621.6 0 106 175 26 621.6 0 26 176 26 621.6 0 26 177 26 621.6 0 26 178 23 621.6 0 23 179 23 621.6 0 23 180 28 621.6 0 28 181 27 621.6 0 27 182 28 621.6 0 28 183 16 621.6 0 16 184 34 621.6 0 34 185 27 621.6 0 27 186 78 621.6 0 78 187 40 621.6 0 40 188 29 621.6 0 29 189 39 621.6 0 39 190 25 621.6 0 25 191 28 621.6 0 28 192 26 621.6 0 26 193 39 621.6 0 39 194 18 621.6 0 18 195 25 621.6 0 25 196 29 621.6 0 29 197 37 621.6 0 37 198 37 621.6 0 37 199 44 621.6 0 44 200 29 621.6 0 29 201 26 621.6 0 26 202 29 621.6 0 29 203 34 621.6 0 34 204 23 621.6 0 23 205 33 621.6 0 33 206 39 621.6 0 39 207 34 621.6 0 34 208 35 621.6 0 35 209 35 621.6 0 35 210 84 621.6 0 84 211 40 621.6 0 40 212 49 621.6 0 49 213 59 621.6 0 59 214 54 621.6 0 54 215 70 621.6 0 70 216 50 621.6 0 50 217 190 621.6 0 190 218 309 621.6 0 309 219 2998 621.6 0 2998 220 115 621.6 0 115 221 38 621.6 0 38 222 55 621.6 0 55 223 65 621.6 0 65 224 77 621.6 0 77 225 55 621.6 0 55 226 225 621.6 0 225 227 261 621.6 0 261 228 256 621.6 0 256 229 44 621.6 0 44 230 42 621.6 0 42 231 34 621.6 0 34 232 38 621.6 0 38 233 32 621.6 0 32 234 42 621.6 0 42 235 22 621.6 0 22 236 26 621.6 0 26 237 35 621.6 0 35 238 40 621.6 0 40 239 33 621.6 0 33 240 30 621.6 0 30 241 42 621.6 0 42 242 38 621.6 0 38 243 34 621.6 0 34 244 28 621.6 0 28 245 27 621.6 0 27 246 50 621.6 0 50 247 63 621.6 0 63 248 60 621.6 0 60 249 36 621.6 0 36 250 50 621.6 0 50 251 37 621.6 0 37 252 44 621.6 0 44 253 42 621.6 0 42 254 32 621.6 0 32 255 36 621.6 0 36 256 28 621.6 0 28 257 33 621.6 0 33 258 28 621.6 0 28 259 34 621.6 0 34 260 34 621.6 0 34 261 38 621.6 0 38 262 43 621.6 0 43 263 50 621.6 0 50 264 42 621.6 0 42 265 31 621.6 0 31 266 49 621.6 0 49 267 34 621.6 0 34 268 35 621.6 0 35 269 27 621.6 0 27 270 25 621.6 0 25 271 35 621.6 0 35 272 49 621.6 0 49 273 51 621.6 0 51 274 58 621.6 0 58 275 54 621.6 0 54 276 61 621.6 0 61 277 82 621.6 0 82 278 45 621.6 0 45 279 32 621.6 0 32 280 48 621.6 0 48 281 57 621.6 0 57 282 33 621.6 0 33 283 48 621.6 0 48 284 34 621.6 0 34 285 45 621.6 0 45 286 25 621.6 0 25 287 58 621.6 0 58 288 51 621.6 0 51 289 40 621.6 0 40 290 69 621.6 0 69 291 52 621.6 0 52 292 80 621.6 0 80 293 56 621.6 0 56 294 53 621.6 0 53 295 35 621.6 0 35 296 47 621.6 0 47 297 50 621.6 0 50 298 73 621.6 0 73 299 66 621.6 0 66 300 92 621.6 0 92 301 56 621.6 0 56 Finished in 17.20 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 383,076 Reads with adapters: 74,503 (19.4%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 74,492 (19.4%) Total basepairs processed: 93,585,323 bp Total written (filtered): 20,310,527 bp (21.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 74503 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 26 0.0 3 0 0 25 1 19 576 0.0 3 0 565 9 2 20 61330 0.0 4 60339 951 38 2 21 230 0.0 4 27 199 3 1 22 12113 0.0 4 11932 167 12 0 2 23 65 0.0 4 4 21 1 36 3 24 4 0.0 4 0 0 2 1 1 25 4 0.0 4 1 0 1 2 27 1 0.0 4 1 34 1 0.0 4 1 39 1 0.0 4 1 41 1 0.0 4 1 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 1 60 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 134 0.0 4 0 0 0 0 134 133 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 1 232 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.64 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 74,492 Reads with adapters: 8,797 (11.8%) Reads written (passing filters): 74,492 (100.0%) Total basepairs processed: 20,310,527 bp Total written (filtered): 20,216,489 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8797 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 2.5% G: 95.2% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 23 1163.9 0 23 4 33 291.0 0 33 5 8 72.7 0 8 6 7 18.2 0 7 7 3 4.5 0 3 9 1007 0.3 0 40 967 10 3295 0.1 1 122 3173 11 4395 0.0 1 17 4378 12 5 0.0 1 0 5 13 3 0.0 1 0 3 42 1 0.0 1 1 76 1 0.0 1 1 148 1 0.0 1 1 150 1 0.0 1 0 1 151 1 0.0 1 0 1 170 3 0.0 1 2 1 208 2 0.0 1 2 209 2 0.0 1 1 1 210 1 0.0 1 1 224 3 0.0 1 3 227 1 0.0 1 1 257 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.