This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.30 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 333,162 (13.1%) Reads that were too short: 6,989 (0.3%) Reads written (passing filters): 326,173 (12.8%) Total basepairs processed: 766,381,518 bp Total written (filtered): 88,242,358 bp (11.5%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 333162 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 10494 621.6 0 10494 7 76909 621.6 0 76909 8 4016 621.6 0 4016 9 91848 621.6 0 91848 10 116 621.6 0 116 11 42 621.6 0 42 12 25 621.6 0 25 13 34 621.6 0 34 14 34 621.6 0 34 15 35 621.6 0 35 16 19 621.6 0 19 17 57 621.6 0 57 18 34 621.6 0 34 19 70 621.6 0 70 20 78 621.6 0 78 21 66 621.6 0 66 22 56 621.6 0 56 23 94 621.6 0 94 24 216 621.6 0 216 25 62 621.6 0 62 26 67 621.6 0 67 27 585 621.6 0 585 28 188 621.6 0 188 29 103 621.6 0 103 30 434 621.6 0 434 31 26 621.6 0 26 32 30 621.6 0 30 33 106 621.6 0 106 34 149 621.6 0 149 35 252 621.6 0 252 36 252 621.6 0 252 37 826 621.6 0 826 38 102 621.6 0 102 39 259 621.6 0 259 40 127 621.6 0 127 41 186 621.6 0 186 42 735 621.6 0 735 43 448 621.6 0 448 44 353 621.6 0 353 45 90 621.6 0 90 46 104 621.6 0 104 47 290 621.6 0 290 48 329 621.6 0 329 49 578 621.6 0 578 50 910 621.6 0 910 51 812 621.6 0 812 52 2010 621.6 0 2010 53 2750 621.6 0 2750 54 16985 621.6 0 16985 55 59964 621.6 0 59964 56 41816 621.6 0 41816 57 192 621.6 0 192 58 45 621.6 0 45 59 32 621.6 0 32 60 34 621.6 0 34 61 19 621.6 0 19 62 19 621.6 0 19 63 20 621.6 0 20 64 16 621.6 0 16 65 11 621.6 0 11 66 15 621.6 0 15 67 17 621.6 0 17 68 36 621.6 0 36 69 30 621.6 0 30 70 21 621.6 0 21 71 22 621.6 0 22 72 20 621.6 0 20 73 26 621.6 0 26 74 19 621.6 0 19 75 20 621.6 0 20 76 14 621.6 0 14 77 25 621.6 0 25 78 18 621.6 0 18 79 25 621.6 0 25 80 18 621.6 0 18 81 52 621.6 0 52 82 33 621.6 0 33 83 25 621.6 0 25 84 33 621.6 0 33 85 26 621.6 0 26 86 11 621.6 0 11 87 26 621.6 0 26 88 22 621.6 0 22 89 23 621.6 0 23 90 26 621.6 0 26 91 26 621.6 0 26 92 35 621.6 0 35 93 43 621.6 0 43 94 269 621.6 0 269 95 464 621.6 0 464 96 712 621.6 0 712 97 12 621.6 0 12 98 21 621.6 0 21 99 18 621.6 0 18 100 19 621.6 0 19 101 25 621.6 0 25 102 24 621.6 0 24 103 17 621.6 0 17 104 28 621.6 0 28 105 33 621.6 0 33 106 7 621.6 0 7 107 25 621.6 0 25 108 35 621.6 0 35 109 297 621.6 0 297 110 286 621.6 0 286 111 474 621.6 0 474 112 15 621.6 0 15 113 13 621.6 0 13 114 11 621.6 0 11 115 22 621.6 0 22 116 26 621.6 0 26 117 37 621.6 0 37 118 45 621.6 0 45 119 102 621.6 0 102 120 128 621.6 0 128 121 424 621.6 0 424 122 148 621.6 0 148 123 140 621.6 0 140 124 18 621.6 0 18 125 25 621.6 0 25 126 31 621.6 0 31 127 26 621.6 0 26 128 19 621.6 0 19 129 27 621.6 0 27 130 45 621.6 0 45 131 25 621.6 0 25 132 21 621.6 0 21 133 38 621.6 0 38 134 29 621.6 0 29 135 94 621.6 0 94 136 286 621.6 0 286 137 90 621.6 0 90 138 76 621.6 0 76 139 72 621.6 0 72 140 84 621.6 0 84 141 210 621.6 0 210 142 36 621.6 0 36 143 27 621.6 0 27 144 32 621.6 0 32 145 61 621.6 0 61 146 31 621.6 0 31 147 63 621.6 0 63 148 114 621.6 0 114 149 73 621.6 0 73 150 61 621.6 0 61 151 36 621.6 0 36 152 35 621.6 0 35 153 36 621.6 0 36 154 28 621.6 0 28 155 30 621.6 0 30 156 42 621.6 0 42 157 47 621.6 0 47 158 32 621.6 0 32 159 43 621.6 0 43 160 101 621.6 0 101 161 132 621.6 0 132 162 197 621.6 0 197 163 106 621.6 0 106 164 34 621.6 0 34 165 236 621.6 0 236 166 222 621.6 0 222 167 170 621.6 0 170 168 71 621.6 0 71 169 35 621.6 0 35 170 26 621.6 0 26 171 30 621.6 0 30 172 32 621.6 0 32 173 42 621.6 0 42 174 45 621.6 0 45 175 126 621.6 0 126 176 133 621.6 0 133 177 100 621.6 0 100 178 85 621.6 0 85 179 44 621.6 0 44 180 117 621.6 0 117 181 88 621.6 0 88 182 225 621.6 0 225 183 36 621.6 0 36 184 22 621.6 0 22 185 49 621.6 0 49 186 32 621.6 0 32 187 29 621.6 0 29 188 55 621.6 0 55 189 19 621.6 0 19 190 18 621.6 0 18 191 36 621.6 0 36 192 35 621.6 0 35 193 34 621.6 0 34 194 39 621.6 0 39 195 25 621.6 0 25 196 26 621.6 0 26 197 25 621.6 0 25 198 29 621.6 0 29 199 33 621.6 0 33 200 28 621.6 0 28 201 20 621.6 0 20 202 33 621.6 0 33 203 55 621.6 0 55 204 61 621.6 0 61 205 63 621.6 0 63 206 38 621.6 0 38 207 39 621.6 0 39 208 44 621.6 0 44 209 50 621.6 0 50 210 49 621.6 0 49 211 43 621.6 0 43 212 30 621.6 0 30 213 43 621.6 0 43 214 40 621.6 0 40 215 44 621.6 0 44 216 33 621.6 0 33 217 34 621.6 0 34 218 38 621.6 0 38 219 44 621.6 0 44 220 43 621.6 0 43 221 40 621.6 0 40 222 49 621.6 0 49 223 101 621.6 0 101 224 87 621.6 0 87 225 70 621.6 0 70 226 50 621.6 0 50 227 43 621.6 0 43 228 59 621.6 0 59 229 88 621.6 0 88 230 85 621.6 0 85 231 50 621.6 0 50 232 41 621.6 0 41 233 27 621.6 0 27 234 20 621.6 0 20 235 25 621.6 0 25 236 35 621.6 0 35 237 40 621.6 0 40 238 33 621.6 0 33 239 45 621.6 0 45 240 43 621.6 0 43 241 27 621.6 0 27 242 39 621.6 0 39 243 31 621.6 0 31 244 30 621.6 0 30 245 37 621.6 0 37 246 44 621.6 0 44 247 52 621.6 0 52 248 56 621.6 0 56 249 43 621.6 0 43 250 30 621.6 0 30 251 37 621.6 0 37 252 53 621.6 0 53 253 72 621.6 0 72 254 83 621.6 0 83 255 87 621.6 0 87 256 149 621.6 0 149 257 166 621.6 0 166 258 48 621.6 0 48 259 78 621.6 0 78 260 65 621.6 0 65 261 63 621.6 0 63 262 190 621.6 0 190 263 161 621.6 0 161 264 272 621.6 0 272 265 45 621.6 0 45 266 39 621.6 0 39 267 51 621.6 0 51 268 36 621.6 0 36 269 38 621.6 0 38 270 41 621.6 0 41 271 56 621.6 0 56 272 53 621.6 0 53 273 71 621.6 0 71 274 57 621.6 0 57 275 84 621.6 0 84 276 48 621.6 0 48 277 51 621.6 0 51 278 246 621.6 0 246 279 185 621.6 0 185 280 182 621.6 0 182 281 59 621.6 0 59 282 130 621.6 0 130 283 139 621.6 0 139 284 161 621.6 0 161 285 59 621.6 0 59 286 42 621.6 0 42 287 44 621.6 0 44 288 40 621.6 0 40 289 64 621.6 0 64 290 50 621.6 0 50 291 53 621.6 0 53 292 108 621.6 0 108 293 55 621.6 0 55 294 74 621.6 0 74 295 56 621.6 0 56 296 44 621.6 0 44 297 44 621.6 0 44 298 41 621.6 0 41 299 84 621.6 0 84 300 408 621.6 0 408 301 183 621.6 0 183 Finished in 14.06 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 326,173 Reads with adapters: 97,442 (29.9%) Reads that were too short: 122 (0.0%) Reads written (passing filters): 97,320 (29.8%) Total basepairs processed: 88,242,358 bp Total written (filtered): 26,737,971 bp (30.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 97442 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 20 0.0 2 0 0 0 20 15 83 0.0 3 0 0 63 20 16 1010 0.0 3 0 856 103 51 17 94681 0.0 3 86909 6311 1176 285 18 259 0.0 3 7 207 29 16 19 962 0.0 3 626 39 152 145 20 81 0.0 3 2 2 0 77 21 4 0.0 3 1 0 2 1 22 2 0.0 3 2 23 5 0.0 3 0 0 0 5 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 6 0.0 3 0 0 0 6 27 1 0.0 3 0 0 0 1 28 5 0.0 3 0 0 0 5 29 1 0.0 3 0 0 0 1 30 10 0.0 3 1 0 0 9 31 1 0.0 3 0 0 0 1 33 4 0.0 3 1 0 1 2 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 1 46 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 5 0.0 3 0 0 0 5 51 1 0.0 3 0 0 0 1 52 4 0.0 3 0 0 1 3 53 1 0.0 3 0 0 0 1 56 13 0.0 3 0 0 2 11 59 3 0.0 3 0 0 1 2 66 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 34 0.0 3 0 0 0 34 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 10 0.0 3 0 0 0 10 98 40 0.0 3 0 0 0 40 99 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 118 6 0.0 3 0 0 0 6 122 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 127 4 0.0 3 1 0 0 3 128 2 0.0 3 0 0 0 2 131 2 0.0 3 0 0 0 2 132 3 0.0 3 0 0 0 3 134 2 0.0 3 0 0 0 2 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 1 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 6 0.0 3 0 0 0 6 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 163 4 0.0 3 0 0 0 4 165 4 0.0 3 0 0 0 4 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 1 1 168 1 0.0 3 0 0 1 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 1 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 198 1 0.0 3 0 0 1 199 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 233 3 0.0 3 0 0 0 3 235 3 0.0 3 0 0 0 3 236 7 0.0 3 0 0 0 7 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 2 0.0 3 0 0 0 2 249 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 279 7 0.0 3 0 0 0 7 280 4 0.0 3 0 0 0 4 281 7 0.0 3 0 0 0 7 282 13 0.0 3 0 0 2 11 283 1 0.0 3 0 0 1 288 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.43 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 97,320 Reads with adapters: 788 (0.8%) Reads written (passing filters): 97,320 (100.0%) Total basepairs processed: 26,737,971 bp Total written (filtered): 26,735,493 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 788 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.5% C: 9.9% G: 55.5% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 691 1520.6 0 691 4 90 380.2 0 90 5 3 95.0 0 3 6 2 23.8 0 2 9 2 0.4 0 0 2