This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.82 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 357,950 (14.1%) Reads that were too short: 4,766 (0.2%) Reads written (passing filters): 353,184 (13.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 95,617,555 bp (12.5%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 357950 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9012 621.6 0 9012 7 97267 621.6 0 97267 8 3150 621.6 0 3150 9 83778 621.6 0 83778 10 86 621.6 0 86 11 38 621.6 0 38 12 25 621.6 0 25 13 26 621.6 0 26 14 42 621.6 0 42 15 49 621.6 0 49 16 26 621.6 0 26 17 53 621.6 0 53 18 53 621.6 0 53 19 77 621.6 0 77 20 98 621.6 0 98 21 72 621.6 0 72 22 55 621.6 0 55 23 111 621.6 0 111 24 272 621.6 0 272 25 86 621.6 0 86 26 68 621.6 0 68 27 723 621.6 0 723 28 61 621.6 0 61 29 54 621.6 0 54 30 65 621.6 0 65 31 45 621.6 0 45 32 31 621.6 0 31 33 59 621.6 0 59 34 133 621.6 0 133 35 167 621.6 0 167 36 326 621.6 0 326 37 924 621.6 0 924 38 127 621.6 0 127 39 286 621.6 0 286 40 168 621.6 0 168 41 196 621.6 0 196 42 935 621.6 0 935 43 558 621.6 0 558 44 464 621.6 0 464 45 111 621.6 0 111 46 128 621.6 0 128 47 264 621.6 0 264 48 331 621.6 0 331 49 535 621.6 0 535 50 827 621.6 0 827 51 770 621.6 0 770 52 2007 621.6 0 2007 53 2565 621.6 0 2565 54 19148 621.6 0 19148 55 69179 621.6 0 69179 56 50243 621.6 0 50243 57 225 621.6 0 225 58 54 621.6 0 54 59 29 621.6 0 29 60 38 621.6 0 38 61 30 621.6 0 30 62 25 621.6 0 25 63 28 621.6 0 28 64 18 621.6 0 18 65 21 621.6 0 21 66 27 621.6 0 27 67 18 621.6 0 18 68 20 621.6 0 20 69 43 621.6 0 43 70 32 621.6 0 32 71 26 621.6 0 26 72 33 621.6 0 33 73 30 621.6 0 30 74 32 621.6 0 32 75 30 621.6 0 30 76 24 621.6 0 24 77 24 621.6 0 24 78 22 621.6 0 22 79 9 621.6 0 9 80 34 621.6 0 34 81 46 621.6 0 46 82 34 621.6 0 34 83 31 621.6 0 31 84 51 621.6 0 51 85 36 621.6 0 36 86 35 621.6 0 35 87 34 621.6 0 34 88 34 621.6 0 34 89 36 621.6 0 36 90 18 621.6 0 18 91 30 621.6 0 30 92 46 621.6 0 46 93 31 621.6 0 31 94 198 621.6 0 198 95 370 621.6 0 370 96 695 621.6 0 695 97 25 621.6 0 25 98 36 621.6 0 36 99 29 621.6 0 29 100 37 621.6 0 37 101 32 621.6 0 32 102 28 621.6 0 28 103 29 621.6 0 29 104 25 621.6 0 25 105 30 621.6 0 30 106 22 621.6 0 22 107 30 621.6 0 30 108 36 621.6 0 36 109 91 621.6 0 91 110 149 621.6 0 149 111 150 621.6 0 150 112 21 621.6 0 21 113 29 621.6 0 29 114 25 621.6 0 25 115 26 621.6 0 26 116 32 621.6 0 32 117 41 621.6 0 41 118 49 621.6 0 49 119 52 621.6 0 52 120 81 621.6 0 81 121 189 621.6 0 189 122 111 621.6 0 111 123 67 621.6 0 67 124 26 621.6 0 26 125 45 621.6 0 45 126 36 621.6 0 36 127 16 621.6 0 16 128 23 621.6 0 23 129 33 621.6 0 33 130 34 621.6 0 34 131 28 621.6 0 28 132 25 621.6 0 25 133 31 621.6 0 31 134 33 621.6 0 33 135 51 621.6 0 51 136 72 621.6 0 72 137 44 621.6 0 44 138 34 621.6 0 34 139 27 621.6 0 27 140 52 621.6 0 52 141 94 621.6 0 94 142 40 621.6 0 40 143 36 621.6 0 36 144 35 621.6 0 35 145 59 621.6 0 59 146 29 621.6 0 29 147 49 621.6 0 49 148 43 621.6 0 43 149 43 621.6 0 43 150 28 621.6 0 28 151 31 621.6 0 31 152 35 621.6 0 35 153 47 621.6 0 47 154 44 621.6 0 44 155 35 621.6 0 35 156 36 621.6 0 36 157 23 621.6 0 23 158 30 621.6 0 30 159 42 621.6 0 42 160 48 621.6 0 48 161 54 621.6 0 54 162 61 621.6 0 61 163 36 621.6 0 36 164 35 621.6 0 35 165 51 621.6 0 51 166 55 621.6 0 55 167 60 621.6 0 60 168 35 621.6 0 35 169 32 621.6 0 32 170 23 621.6 0 23 171 33 621.6 0 33 172 27 621.6 0 27 173 39 621.6 0 39 174 45 621.6 0 45 175 63 621.6 0 63 176 65 621.6 0 65 177 69 621.6 0 69 178 54 621.6 0 54 179 27 621.6 0 27 180 59 621.6 0 59 181 56 621.6 0 56 182 88 621.6 0 88 183 37 621.6 0 37 184 30 621.6 0 30 185 52 621.6 0 52 186 31 621.6 0 31 187 28 621.6 0 28 188 72 621.6 0 72 189 35 621.6 0 35 190 30 621.6 0 30 191 27 621.6 0 27 192 51 621.6 0 51 193 41 621.6 0 41 194 49 621.6 0 49 195 36 621.6 0 36 196 41 621.6 0 41 197 41 621.6 0 41 198 35 621.6 0 35 199 46 621.6 0 46 200 29 621.6 0 29 201 26 621.6 0 26 202 23 621.6 0 23 203 57 621.6 0 57 204 49 621.6 0 49 205 52 621.6 0 52 206 41 621.6 0 41 207 37 621.6 0 37 208 40 621.6 0 40 209 26 621.6 0 26 210 20 621.6 0 20 211 35 621.6 0 35 212 43 621.6 0 43 213 32 621.6 0 32 214 25 621.6 0 25 215 28 621.6 0 28 216 32 621.6 0 32 217 38 621.6 0 38 218 31 621.6 0 31 219 37 621.6 0 37 220 33 621.6 0 33 221 32 621.6 0 32 222 44 621.6 0 44 223 53 621.6 0 53 224 37 621.6 0 37 225 57 621.6 0 57 226 34 621.6 0 34 227 38 621.6 0 38 228 61 621.6 0 61 229 43 621.6 0 43 230 39 621.6 0 39 231 34 621.6 0 34 232 29 621.6 0 29 233 35 621.6 0 35 234 26 621.6 0 26 235 29 621.6 0 29 236 37 621.6 0 37 237 38 621.6 0 38 238 27 621.6 0 27 239 29 621.6 0 29 240 36 621.6 0 36 241 36 621.6 0 36 242 33 621.6 0 33 243 26 621.6 0 26 244 31 621.6 0 31 245 16 621.6 0 16 246 25 621.6 0 25 247 43 621.6 0 43 248 36 621.6 0 36 249 35 621.6 0 35 250 34 621.6 0 34 251 15 621.6 0 15 252 43 621.6 0 43 253 33 621.6 0 33 254 50 621.6 0 50 255 41 621.6 0 41 256 55 621.6 0 55 257 129 621.6 0 129 258 40 621.6 0 40 259 56 621.6 0 56 260 42 621.6 0 42 261 37 621.6 0 37 262 71 621.6 0 71 263 55 621.6 0 55 264 71 621.6 0 71 265 41 621.6 0 41 266 39 621.6 0 39 267 35 621.6 0 35 268 24 621.6 0 24 269 45 621.6 0 45 270 26 621.6 0 26 271 53 621.6 0 53 272 54 621.6 0 54 273 62 621.6 0 62 274 39 621.6 0 39 275 87 621.6 0 87 276 47 621.6 0 47 277 31 621.6 0 31 278 110 621.6 0 110 279 85 621.6 0 85 280 76 621.6 0 76 281 36 621.6 0 36 282 45 621.6 0 45 283 45 621.6 0 45 284 72 621.6 0 72 285 34 621.6 0 34 286 24 621.6 0 24 287 38 621.6 0 38 288 30 621.6 0 30 289 35 621.6 0 35 290 34 621.6 0 34 291 55 621.6 0 55 292 41 621.6 0 41 293 55 621.6 0 55 294 47 621.6 0 47 295 49 621.6 0 49 296 53 621.6 0 53 297 35 621.6 0 35 298 44 621.6 0 44 299 75 621.6 0 75 300 437 621.6 0 437 301 138 621.6 0 138 Finished in 16.52 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 353,184 Reads with adapters: 104,987 (29.7%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 104,975 (29.7%) Total basepairs processed: 95,617,555 bp Total written (filtered): 28,741,346 bp (30.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 104987 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 0 1 18 32 0.0 3 0 0 30 2 19 920 0.0 3 0 903 17 20 103432 0.0 4 101964 1412 51 3 2 21 219 0.0 4 28 185 6 22 19 0.0 4 2 0 16 0 1 23 72 0.0 4 1 0 1 67 3 24 2 0.0 4 0 0 0 0 2 25 2 0.0 4 2 37 1 0.0 4 1 38 1 0.0 4 1 43 1 0.0 4 1 50 1 0.0 4 0 1 56 1 0.0 4 1 82 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 103 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 9 0.0 4 0 0 0 0 9 127 253 0.0 4 0 0 0 0 253 129 4 0.0 4 0 0 0 0 4 169 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 193 1 0.0 4 1 214 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 1 1 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.63 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 104,975 Reads with adapters: 7,313 (7.0%) Reads written (passing filters): 104,975 (100.0%) Total basepairs processed: 28,741,346 bp Total written (filtered): 28,659,999 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7313 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 2.5% G: 94.7% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 115 1640.2 0 115 4 21 410.1 0 21 5 9 102.5 0 9 6 3 25.6 0 3 9 14 0.4 0 2 12 10 21 0.1 1 1 20 11 6984 0.0 1 32 6952 12 122 0.0 1 5 117 13 6 0.0 1 0 6 14 2 0.0 1 0 2 15 4 0.0 1 0 4 149 1 0.0 1 1 152 7 0.0 1 0 7 191 1 0.0 1 1 210 1 0.0 1 1 223 1 0.0 1 0 1 228 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.