This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.35 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 89,977 (3.5%) Reads that were too short: 14,864 (0.6%) Reads written (passing filters): 75,113 (3.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 18,862,245 bp (2.5%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 89977 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 464 621.6 0 464 7 705 621.6 0 705 8 24287 621.6 0 24287 9 20138 621.6 0 20138 10 70 621.6 0 70 11 67 621.6 0 67 12 71 621.6 0 71 13 86 621.6 0 86 14 61 621.6 0 61 15 53 621.6 0 53 16 61 621.6 0 61 17 65 621.6 0 65 18 44 621.6 0 44 19 44 621.6 0 44 20 50 621.6 0 50 21 52 621.6 0 52 22 79 621.6 0 79 23 59 621.6 0 59 24 60 621.6 0 60 25 58 621.6 0 58 26 52 621.6 0 52 27 74 621.6 0 74 28 71 621.6 0 71 29 51 621.6 0 51 30 55 621.6 0 55 31 64 621.6 0 64 32 83 621.6 0 83 33 55 621.6 0 55 34 58 621.6 0 58 35 71 621.6 0 71 36 48 621.6 0 48 37 56 621.6 0 56 38 76 621.6 0 76 39 55 621.6 0 55 40 54 621.6 0 54 41 53 621.6 0 53 42 67 621.6 0 67 43 62 621.6 0 62 44 61 621.6 0 61 45 103 621.6 0 103 46 69 621.6 0 69 47 124 621.6 0 124 48 45 621.6 0 45 49 79 621.6 0 79 50 63 621.6 0 63 51 43 621.6 0 43 52 51 621.6 0 51 53 56 621.6 0 56 54 35 621.6 0 35 55 56 621.6 0 56 56 66 621.6 0 66 57 61 621.6 0 61 58 49 621.6 0 49 59 60 621.6 0 60 60 72 621.6 0 72 61 66 621.6 0 66 62 72 621.6 0 72 63 70 621.6 0 70 64 64 621.6 0 64 65 78 621.6 0 78 66 116 621.6 0 116 67 59 621.6 0 59 68 93 621.6 0 93 69 112 621.6 0 112 70 97 621.6 0 97 71 104 621.6 0 104 72 121 621.6 0 121 73 116 621.6 0 116 74 370 621.6 0 370 75 202 621.6 0 202 76 184 621.6 0 184 77 183 621.6 0 183 78 193 621.6 0 193 79 1168 621.6 0 1168 80 1355 621.6 0 1355 81 3778 621.6 0 3778 82 164 621.6 0 164 83 181 621.6 0 181 84 203 621.6 0 203 85 68 621.6 0 68 86 57 621.6 0 57 87 69 621.6 0 69 88 74 621.6 0 74 89 67 621.6 0 67 90 57 621.6 0 57 91 62 621.6 0 62 92 65 621.6 0 65 93 71 621.6 0 71 94 65 621.6 0 65 95 85 621.6 0 85 96 74 621.6 0 74 97 89 621.6 0 89 98 72 621.6 0 72 99 71 621.6 0 71 100 200 621.6 0 200 101 187 621.6 0 187 102 98 621.6 0 98 103 94 621.6 0 94 104 124 621.6 0 124 105 93 621.6 0 93 106 104 621.6 0 104 107 121 621.6 0 121 108 138 621.6 0 138 109 100 621.6 0 100 110 93 621.6 0 93 111 109 621.6 0 109 112 129 621.6 0 129 113 134 621.6 0 134 114 105 621.6 0 105 115 125 621.6 0 125 116 148 621.6 0 148 117 128 621.6 0 128 118 110 621.6 0 110 119 116 621.6 0 116 120 131 621.6 0 131 121 136 621.6 0 136 122 121 621.6 0 121 123 115 621.6 0 115 124 217 621.6 0 217 125 516 621.6 0 516 126 369 621.6 0 369 127 497 621.6 0 497 128 245 621.6 0 245 129 204 621.6 0 204 130 212 621.6 0 212 131 115 621.6 0 115 132 108 621.6 0 108 133 100 621.6 0 100 134 106 621.6 0 106 135 108 621.6 0 108 136 136 621.6 0 136 137 175 621.6 0 175 138 170 621.6 0 170 139 289 621.6 0 289 140 154 621.6 0 154 141 153 621.6 0 153 142 131 621.6 0 131 143 229 621.6 0 229 144 122 621.6 0 122 145 92 621.6 0 92 146 124 621.6 0 124 147 96 621.6 0 96 148 102 621.6 0 102 149 98 621.6 0 98 150 356 621.6 0 356 151 203 621.6 0 203 152 240 621.6 0 240 153 73 621.6 0 73 154 91 621.6 0 91 155 71 621.6 0 71 156 95 621.6 0 95 157 95 621.6 0 95 158 162 621.6 0 162 159 157 621.6 0 157 160 197 621.6 0 197 161 115 621.6 0 115 162 102 621.6 0 102 163 122 621.6 0 122 164 211 621.6 0 211 165 157 621.6 0 157 166 116 621.6 0 116 167 100 621.6 0 100 168 84 621.6 0 84 169 96 621.6 0 96 170 89 621.6 0 89 171 81 621.6 0 81 172 71 621.6 0 71 173 78 621.6 0 78 174 79 621.6 0 79 175 75 621.6 0 75 176 82 621.6 0 82 177 124 621.6 0 124 178 88 621.6 0 88 179 90 621.6 0 90 180 139 621.6 0 139 181 159 621.6 0 159 182 955 621.6 0 955 183 576 621.6 0 576 184 1456 621.6 0 1456 185 100 621.6 0 100 186 66 621.6 0 66 187 74 621.6 0 74 188 72 621.6 0 72 189 88 621.6 0 88 190 71 621.6 0 71 191 76 621.6 0 76 192 62 621.6 0 62 193 67 621.6 0 67 194 66 621.6 0 66 195 75 621.6 0 75 196 74 621.6 0 74 197 74 621.6 0 74 198 85 621.6 0 85 199 91 621.6 0 91 200 95 621.6 0 95 201 98 621.6 0 98 202 67 621.6 0 67 203 99 621.6 0 99 204 105 621.6 0 105 205 147 621.6 0 147 206 147 621.6 0 147 207 132 621.6 0 132 208 171 621.6 0 171 209 180 621.6 0 180 210 145 621.6 0 145 211 128 621.6 0 128 212 154 621.6 0 154 213 141 621.6 0 141 214 89 621.6 0 89 215 115 621.6 0 115 216 104 621.6 0 104 217 98 621.6 0 98 218 147 621.6 0 147 219 109 621.6 0 109 220 169 621.6 0 169 221 120 621.6 0 120 222 131 621.6 0 131 223 130 621.6 0 130 224 156 621.6 0 156 225 266 621.6 0 266 226 520 621.6 0 520 227 597 621.6 0 597 228 329 621.6 0 329 229 88 621.6 0 88 230 98 621.6 0 98 231 241 621.6 0 241 232 621 621.6 0 621 233 179 621.6 0 179 234 91 621.6 0 91 235 100 621.6 0 100 236 93 621.6 0 93 237 89 621.6 0 89 238 110 621.6 0 110 239 106 621.6 0 106 240 107 621.6 0 107 241 113 621.6 0 113 242 84 621.6 0 84 243 80 621.6 0 80 244 85 621.6 0 85 245 67 621.6 0 67 246 92 621.6 0 92 247 109 621.6 0 109 248 110 621.6 0 110 249 113 621.6 0 113 250 108 621.6 0 108 251 117 621.6 0 117 252 104 621.6 0 104 253 217 621.6 0 217 254 388 621.6 0 388 255 616 621.6 0 616 256 234 621.6 0 234 257 95 621.6 0 95 258 84 621.6 0 84 259 92 621.6 0 92 260 113 621.6 0 113 261 167 621.6 0 167 262 114 621.6 0 114 263 216 621.6 0 216 264 568 621.6 0 568 265 309 621.6 0 309 266 358 621.6 0 358 267 83 621.6 0 83 268 89 621.6 0 89 269 112 621.6 0 112 270 114 621.6 0 114 271 87 621.6 0 87 272 85 621.6 0 85 273 103 621.6 0 103 274 101 621.6 0 101 275 139 621.6 0 139 276 92 621.6 0 92 277 122 621.6 0 122 278 86 621.6 0 86 279 100 621.6 0 100 280 104 621.6 0 104 281 83 621.6 0 83 282 96 621.6 0 96 283 76 621.6 0 76 284 102 621.6 0 102 285 105 621.6 0 105 286 82 621.6 0 82 287 82 621.6 0 82 288 121 621.6 0 121 289 134 621.6 0 134 290 122 621.6 0 122 291 102 621.6 0 102 292 88 621.6 0 88 293 107 621.6 0 107 294 78 621.6 0 78 295 75 621.6 0 75 296 89 621.6 0 89 297 89 621.6 0 89 298 106 621.6 0 106 299 115 621.6 0 115 300 76 621.6 0 76 301 147 621.6 0 147 Finished in 3.34 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 75,113 Reads with adapters: 24,302 (32.4%) Reads that were too short: 103 (0.1%) Reads written (passing filters): 24,199 (32.2%) Total basepairs processed: 18,862,245 bp Total written (filtered): 6,652,922 bp (35.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24302 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 26 0.0 3 0 0 19 7 16 299 0.0 3 0 260 24 15 17 23469 0.0 3 21583 1503 313 70 18 111 0.0 3 38 48 13 12 19 113 0.0 3 14 4 56 39 20 13 0.0 3 0 0 0 13 21 5 0.0 3 0 0 1 4 22 1 0.0 3 0 0 0 1 25 4 0.0 3 0 0 0 4 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 6 0.0 3 0 0 0 6 31 4 0.0 3 0 0 0 4 32 2 0.0 3 0 0 0 2 41 28 0.0 3 0 0 1 27 43 1 0.0 3 0 0 0 1 44 4 0.0 3 0 0 0 4 50 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 52 2 0.0 3 0 0 1 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 65 9 0.0 3 0 0 0 9 66 2 0.0 3 0 0 0 2 67 7 0.0 3 0 0 0 7 69 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 101 6 0.0 3 0 0 0 6 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 1 1 112 51 0.0 3 0 0 0 51 113 2 0.0 3 0 0 0 2 115 7 0.0 3 0 0 0 7 117 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 9 0.0 3 0 0 0 9 173 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 213 3 0.0 3 0 0 0 3 214 16 0.0 3 0 0 0 16 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 1 259 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 281 1 0.0 3 0 0 0 1 283 3 0.0 3 0 0 0 3 285 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.84 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 24,199 Reads with adapters: 31 (0.1%) Reads written (passing filters): 24,199 (100.0%) Total basepairs processed: 6,652,922 bp Total written (filtered): 6,652,807 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 31 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.9% C: 16.1% G: 61.3% T: 9.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 378.1 0 11 4 18 94.5 0 18 5 2 23.6 0 2