This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.69 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 99,940 (3.9%) Reads that were too short: 16,898 (0.7%) Reads written (passing filters): 83,042 (3.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 20,596,023 bp (2.7%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 99940 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 364 621.6 0 364 7 537 621.6 0 537 8 22295 621.6 0 22295 9 24283 621.6 0 24283 10 89 621.6 0 89 11 107 621.6 0 107 12 108 621.6 0 108 13 94 621.6 0 94 14 122 621.6 0 122 15 73 621.6 0 73 16 96 621.6 0 96 17 96 621.6 0 96 18 75 621.6 0 75 19 69 621.6 0 69 20 85 621.6 0 85 21 89 621.6 0 89 22 86 621.6 0 86 23 85 621.6 0 85 24 99 621.6 0 99 25 84 621.6 0 84 26 100 621.6 0 100 27 96 621.6 0 96 28 95 621.6 0 95 29 109 621.6 0 109 30 72 621.6 0 72 31 97 621.6 0 97 32 105 621.6 0 105 33 65 621.6 0 65 34 96 621.6 0 96 35 121 621.6 0 121 36 83 621.6 0 83 37 86 621.6 0 86 38 159 621.6 0 159 39 94 621.6 0 94 40 85 621.6 0 85 41 112 621.6 0 112 42 85 621.6 0 85 43 89 621.6 0 89 44 104 621.6 0 104 45 150 621.6 0 150 46 104 621.6 0 104 47 132 621.6 0 132 48 90 621.6 0 90 49 111 621.6 0 111 50 88 621.6 0 88 51 91 621.6 0 91 52 79 621.6 0 79 53 70 621.6 0 70 54 83 621.6 0 83 55 88 621.6 0 88 56 109 621.6 0 109 57 108 621.6 0 108 58 74 621.6 0 74 59 93 621.6 0 93 60 114 621.6 0 114 61 85 621.6 0 85 62 117 621.6 0 117 63 102 621.6 0 102 64 100 621.6 0 100 65 127 621.6 0 127 66 157 621.6 0 157 67 126 621.6 0 126 68 148 621.6 0 148 69 148 621.6 0 148 70 134 621.6 0 134 71 127 621.6 0 127 72 122 621.6 0 122 73 110 621.6 0 110 74 110 621.6 0 110 75 146 621.6 0 146 76 161 621.6 0 161 77 170 621.6 0 170 78 208 621.6 0 208 79 1237 621.6 0 1237 80 1415 621.6 0 1415 81 3762 621.6 0 3762 82 208 621.6 0 208 83 206 621.6 0 206 84 235 621.6 0 235 85 157 621.6 0 157 86 135 621.6 0 135 87 168 621.6 0 168 88 190 621.6 0 190 89 159 621.6 0 159 90 131 621.6 0 131 91 162 621.6 0 162 92 170 621.6 0 170 93 135 621.6 0 135 94 109 621.6 0 109 95 120 621.6 0 120 96 176 621.6 0 176 97 148 621.6 0 148 98 172 621.6 0 172 99 162 621.6 0 162 100 154 621.6 0 154 101 151 621.6 0 151 102 168 621.6 0 168 103 157 621.6 0 157 104 157 621.6 0 157 105 163 621.6 0 163 106 152 621.6 0 152 107 170 621.6 0 170 108 198 621.6 0 198 109 189 621.6 0 189 110 143 621.6 0 143 111 175 621.6 0 175 112 172 621.6 0 172 113 145 621.6 0 145 114 142 621.6 0 142 115 164 621.6 0 164 116 191 621.6 0 191 117 150 621.6 0 150 118 138 621.6 0 138 119 160 621.6 0 160 120 150 621.6 0 150 121 148 621.6 0 148 122 137 621.6 0 137 123 129 621.6 0 129 124 208 621.6 0 208 125 282 621.6 0 282 126 256 621.6 0 256 127 310 621.6 0 310 128 242 621.6 0 242 129 196 621.6 0 196 130 262 621.6 0 262 131 141 621.6 0 141 132 149 621.6 0 149 133 128 621.6 0 128 134 138 621.6 0 138 135 161 621.6 0 161 136 188 621.6 0 188 137 172 621.6 0 172 138 178 621.6 0 178 139 320 621.6 0 320 140 208 621.6 0 208 141 208 621.6 0 208 142 183 621.6 0 183 143 256 621.6 0 256 144 175 621.6 0 175 145 129 621.6 0 129 146 192 621.6 0 192 147 141 621.6 0 141 148 109 621.6 0 109 149 115 621.6 0 115 150 253 621.6 0 253 151 219 621.6 0 219 152 218 621.6 0 218 153 124 621.6 0 124 154 154 621.6 0 154 155 132 621.6 0 132 156 150 621.6 0 150 157 134 621.6 0 134 158 198 621.6 0 198 159 235 621.6 0 235 160 249 621.6 0 249 161 141 621.6 0 141 162 151 621.6 0 151 163 145 621.6 0 145 164 295 621.6 0 295 165 145 621.6 0 145 166 187 621.6 0 187 167 154 621.6 0 154 168 121 621.6 0 121 169 142 621.6 0 142 170 162 621.6 0 162 171 143 621.6 0 143 172 128 621.6 0 128 173 129 621.6 0 129 174 133 621.6 0 133 175 146 621.6 0 146 176 144 621.6 0 144 177 185 621.6 0 185 178 177 621.6 0 177 179 144 621.6 0 144 180 139 621.6 0 139 181 198 621.6 0 198 182 744 621.6 0 744 183 517 621.6 0 517 184 1053 621.6 0 1053 185 151 621.6 0 151 186 98 621.6 0 98 187 139 621.6 0 139 188 122 621.6 0 122 189 152 621.6 0 152 190 121 621.6 0 121 191 145 621.6 0 145 192 147 621.6 0 147 193 124 621.6 0 124 194 135 621.6 0 135 195 140 621.6 0 140 196 131 621.6 0 131 197 154 621.6 0 154 198 137 621.6 0 137 199 136 621.6 0 136 200 196 621.6 0 196 201 147 621.6 0 147 202 114 621.6 0 114 203 160 621.6 0 160 204 142 621.6 0 142 205 160 621.6 0 160 206 151 621.6 0 151 207 162 621.6 0 162 208 243 621.6 0 243 209 183 621.6 0 183 210 161 621.6 0 161 211 143 621.6 0 143 212 171 621.6 0 171 213 152 621.6 0 152 214 130 621.6 0 130 215 176 621.6 0 176 216 142 621.6 0 142 217 137 621.6 0 137 218 187 621.6 0 187 219 159 621.6 0 159 220 183 621.6 0 183 221 140 621.6 0 140 222 179 621.6 0 179 223 151 621.6 0 151 224 155 621.6 0 155 225 221 621.6 0 221 226 399 621.6 0 399 227 457 621.6 0 457 228 257 621.6 0 257 229 148 621.6 0 148 230 122 621.6 0 122 231 248 621.6 0 248 232 441 621.6 0 441 233 223 621.6 0 223 234 101 621.6 0 101 235 183 621.6 0 183 236 144 621.6 0 144 237 141 621.6 0 141 238 165 621.6 0 165 239 148 621.6 0 148 240 133 621.6 0 133 241 172 621.6 0 172 242 141 621.6 0 141 243 144 621.6 0 144 244 107 621.6 0 107 245 130 621.6 0 130 246 157 621.6 0 157 247 156 621.6 0 156 248 178 621.6 0 178 249 158 621.6 0 158 250 143 621.6 0 143 251 131 621.6 0 131 252 127 621.6 0 127 253 253 621.6 0 253 254 450 621.6 0 450 255 749 621.6 0 749 256 340 621.6 0 340 257 183 621.6 0 183 258 123 621.6 0 123 259 139 621.6 0 139 260 150 621.6 0 150 261 153 621.6 0 153 262 142 621.6 0 142 263 153 621.6 0 153 264 257 621.6 0 257 265 192 621.6 0 192 266 188 621.6 0 188 267 116 621.6 0 116 268 137 621.6 0 137 269 133 621.6 0 133 270 132 621.6 0 132 271 123 621.6 0 123 272 132 621.6 0 132 273 125 621.6 0 125 274 128 621.6 0 128 275 154 621.6 0 154 276 136 621.6 0 136 277 156 621.6 0 156 278 133 621.6 0 133 279 104 621.6 0 104 280 145 621.6 0 145 281 132 621.6 0 132 282 121 621.6 0 121 283 112 621.6 0 112 284 166 621.6 0 166 285 130 621.6 0 130 286 127 621.6 0 127 287 121 621.6 0 121 288 113 621.6 0 113 289 121 621.6 0 121 290 125 621.6 0 125 291 87 621.6 0 87 292 105 621.6 0 105 293 116 621.6 0 116 294 127 621.6 0 127 295 129 621.6 0 129 296 138 621.6 0 138 297 137 621.6 0 137 298 92 621.6 0 92 299 141 621.6 0 141 300 126 621.6 0 126 301 150 621.6 0 150 Finished in 3.98 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 83,042 Reads with adapters: 25,326 (30.5%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 25,312 (30.5%) Total basepairs processed: 20,596,023 bp Total written (filtered): 6,885,267 bp (33.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25326 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 8 0.0 3 0 0 8 19 317 0.0 3 0 309 8 20 24882 0.0 4 24469 399 12 2 21 62 0.0 4 13 49 22 10 0.0 4 2 0 6 0 2 23 25 0.0 4 2 0 0 21 2 24 1 0.0 4 1 49 1 0.0 4 1 65 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 77 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 111 1 0.0 4 1 122 1 0.0 4 1 133 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.94 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 25,312 Reads with adapters: 7,695 (30.4%) Reads written (passing filters): 25,312 (100.0%) Total basepairs processed: 6,885,267 bp Total written (filtered): 6,813,343 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7695 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.4% C: 1.5% G: 97.3% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 395.5 0 5 4 5 98.9 0 5 9 6435 0.1 0 177 6258 10 1049 0.0 1 35 1014 11 181 0.0 1 0 181 12 12 0.0 1 0 12 150 4 0.0 1 0 4 151 1 0.0 1 0 1 189 2 0.0 1 1 1 220 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.