This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.76 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 212,279 (8.3%) Reads that were too short: 11,306 (0.4%) Reads written (passing filters): 200,973 (7.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 57,280,340 bp (7.5%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 212279 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3204 621.6 0 3204 7 96952 621.6 0 96952 8 84829 621.6 0 84829 9 129 621.6 0 129 10 53 621.6 0 53 11 64 621.6 0 64 12 55 621.6 0 55 13 69 621.6 0 69 14 67 621.6 0 67 15 51 621.6 0 51 16 92 621.6 0 92 17 54 621.6 0 54 18 94 621.6 0 94 19 51 621.6 0 51 20 44 621.6 0 44 21 44 621.6 0 44 22 41 621.6 0 41 23 55 621.6 0 55 24 64 621.6 0 64 25 58 621.6 0 58 26 171 621.6 0 171 27 97 621.6 0 97 28 168 621.6 0 168 29 36 621.6 0 36 30 50 621.6 0 50 31 64 621.6 0 64 32 68 621.6 0 68 33 48 621.6 0 48 34 44 621.6 0 44 35 45 621.6 0 45 36 51 621.6 0 51 37 58 621.6 0 58 38 39 621.6 0 39 39 55 621.6 0 55 40 56 621.6 0 56 41 55 621.6 0 55 42 74 621.6 0 74 43 49 621.6 0 49 44 44 621.6 0 44 45 36 621.6 0 36 46 50 621.6 0 50 47 40 621.6 0 40 48 42 621.6 0 42 49 45 621.6 0 45 50 44 621.6 0 44 51 49 621.6 0 49 52 49 621.6 0 49 53 43 621.6 0 43 54 41 621.6 0 41 55 26 621.6 0 26 56 32 621.6 0 32 57 57 621.6 0 57 58 48 621.6 0 48 59 59 621.6 0 59 60 38 621.6 0 38 61 36 621.6 0 36 62 43 621.6 0 43 63 63 621.6 0 63 64 51 621.6 0 51 65 54 621.6 0 54 66 40 621.6 0 40 67 39 621.6 0 39 68 39 621.6 0 39 69 56 621.6 0 56 70 36 621.6 0 36 71 44 621.6 0 44 72 46 621.6 0 46 73 48 621.6 0 48 74 45 621.6 0 45 75 93 621.6 0 93 76 74 621.6 0 74 77 89 621.6 0 89 78 61 621.6 0 61 79 53 621.6 0 53 80 61 621.6 0 61 81 62 621.6 0 62 82 47 621.6 0 47 83 54 621.6 0 54 84 56 621.6 0 56 85 43 621.6 0 43 86 41 621.6 0 41 87 68 621.6 0 68 88 45 621.6 0 45 89 58 621.6 0 58 90 46 621.6 0 46 91 62 621.6 0 62 92 64 621.6 0 64 93 45 621.6 0 45 94 47 621.6 0 47 95 56 621.6 0 56 96 93 621.6 0 93 97 62 621.6 0 62 98 59 621.6 0 59 99 85 621.6 0 85 100 161 621.6 0 161 101 91 621.6 0 91 102 99 621.6 0 99 103 108 621.6 0 108 104 71 621.6 0 71 105 85 621.6 0 85 106 120 621.6 0 120 107 115 621.6 0 115 108 509 621.6 0 509 109 268 621.6 0 268 110 562 621.6 0 562 111 89 621.6 0 89 112 82 621.6 0 82 113 106 621.6 0 106 114 147 621.6 0 147 115 117 621.6 0 117 116 147 621.6 0 147 117 83 621.6 0 83 118 80 621.6 0 80 119 86 621.6 0 86 120 66 621.6 0 66 121 78 621.6 0 78 122 97 621.6 0 97 123 112 621.6 0 112 124 69 621.6 0 69 125 69 621.6 0 69 126 66 621.6 0 66 127 59 621.6 0 59 128 107 621.6 0 107 129 102 621.6 0 102 130 99 621.6 0 99 131 123 621.6 0 123 132 89 621.6 0 89 133 96 621.6 0 96 134 97 621.6 0 97 135 101 621.6 0 101 136 145 621.6 0 145 137 93 621.6 0 93 138 103 621.6 0 103 139 86 621.6 0 86 140 86 621.6 0 86 141 110 621.6 0 110 142 105 621.6 0 105 143 121 621.6 0 121 144 110 621.6 0 110 145 104 621.6 0 104 146 98 621.6 0 98 147 75 621.6 0 75 148 79 621.6 0 79 149 78 621.6 0 78 150 75 621.6 0 75 151 62 621.6 0 62 152 62 621.6 0 62 153 79 621.6 0 79 154 86 621.6 0 86 155 139 621.6 0 139 156 70 621.6 0 70 157 177 621.6 0 177 158 92 621.6 0 92 159 120 621.6 0 120 160 75 621.6 0 75 161 88 621.6 0 88 162 105 621.6 0 105 163 121 621.6 0 121 164 135 621.6 0 135 165 154 621.6 0 154 166 140 621.6 0 140 167 117 621.6 0 117 168 74 621.6 0 74 169 104 621.6 0 104 170 85 621.6 0 85 171 59 621.6 0 59 172 90 621.6 0 90 173 96 621.6 0 96 174 59 621.6 0 59 175 89 621.6 0 89 176 104 621.6 0 104 177 72 621.6 0 72 178 107 621.6 0 107 179 110 621.6 0 110 180 88 621.6 0 88 181 87 621.6 0 87 182 105 621.6 0 105 183 97 621.6 0 97 184 97 621.6 0 97 185 87 621.6 0 87 186 85 621.6 0 85 187 62 621.6 0 62 188 89 621.6 0 89 189 72 621.6 0 72 190 109 621.6 0 109 191 82 621.6 0 82 192 79 621.6 0 79 193 65 621.6 0 65 194 73 621.6 0 73 195 70 621.6 0 70 196 73 621.6 0 73 197 81 621.6 0 81 198 78 621.6 0 78 199 82 621.6 0 82 200 107 621.6 0 107 201 105 621.6 0 105 202 123 621.6 0 123 203 116 621.6 0 116 204 94 621.6 0 94 205 98 621.6 0 98 206 93 621.6 0 93 207 91 621.6 0 91 208 173 621.6 0 173 209 133 621.6 0 133 210 107 621.6 0 107 211 105 621.6 0 105 212 81 621.6 0 81 213 91 621.6 0 91 214 84 621.6 0 84 215 79 621.6 0 79 216 114 621.6 0 114 217 96 621.6 0 96 218 80 621.6 0 80 219 81 621.6 0 81 220 88 621.6 0 88 221 102 621.6 0 102 222 151 621.6 0 151 223 197 621.6 0 197 224 192 621.6 0 192 225 105 621.6 0 105 226 100 621.6 0 100 227 79 621.6 0 79 228 68 621.6 0 68 229 74 621.6 0 74 230 61 621.6 0 61 231 70 621.6 0 70 232 96 621.6 0 96 233 48 621.6 0 48 234 69 621.6 0 69 235 83 621.6 0 83 236 68 621.6 0 68 237 90 621.6 0 90 238 81 621.6 0 81 239 80 621.6 0 80 240 94 621.6 0 94 241 80 621.6 0 80 242 90 621.6 0 90 243 138 621.6 0 138 244 186 621.6 0 186 245 125 621.6 0 125 246 160 621.6 0 160 247 86 621.6 0 86 248 64 621.6 0 64 249 87 621.6 0 87 250 70 621.6 0 70 251 80 621.6 0 80 252 140 621.6 0 140 253 334 621.6 0 334 254 257 621.6 0 257 255 555 621.6 0 555 256 82 621.6 0 82 257 73 621.6 0 73 258 99 621.6 0 99 259 102 621.6 0 102 260 121 621.6 0 121 261 79 621.6 0 79 262 122 621.6 0 122 263 244 621.6 0 244 264 217 621.6 0 217 265 234 621.6 0 234 266 89 621.6 0 89 267 62 621.6 0 62 268 53 621.6 0 53 269 86 621.6 0 86 270 82 621.6 0 82 271 185 621.6 0 185 272 104 621.6 0 104 273 136 621.6 0 136 274 76 621.6 0 76 275 80 621.6 0 80 276 124 621.6 0 124 277 79 621.6 0 79 278 70 621.6 0 70 279 70 621.6 0 70 280 88 621.6 0 88 281 107 621.6 0 107 282 97 621.6 0 97 283 120 621.6 0 120 284 145 621.6 0 145 285 167 621.6 0 167 286 101 621.6 0 101 287 89 621.6 0 89 288 65 621.6 0 65 289 94 621.6 0 94 290 132 621.6 0 132 291 180 621.6 0 180 292 187 621.6 0 187 293 84 621.6 0 84 294 93 621.6 0 93 295 63 621.6 0 63 296 86 621.6 0 86 297 82 621.6 0 82 298 91 621.6 0 91 299 99 621.6 0 99 300 93 621.6 0 93 301 87 621.6 0 87 Finished in 9.34 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 200,973 Reads with adapters: 97,433 (48.5%) Reads that were too short: 67 (0.0%) Reads written (passing filters): 97,366 (48.4%) Total basepairs processed: 57,280,340 bp Total written (filtered): 26,963,372 bp (47.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 97433 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 25 0.0 2 0 0 0 25 15 119 0.0 3 0 0 98 21 16 1255 0.0 3 0 1042 138 75 17 95154 0.0 3 87598 6063 1188 305 18 263 0.0 3 18 191 27 27 19 400 0.0 3 13 3 209 175 20 81 0.0 3 2 1 1 77 21 2 0.0 3 1 0 1 23 1 0.0 3 1 24 2 0.0 3 2 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 1 28 1 0.0 3 0 0 0 1 29 4 0.0 3 1 0 0 3 30 1 0.0 3 0 0 0 1 32 2 0.0 3 1 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 58 4 0.0 3 0 0 0 4 60 8 0.0 3 0 0 0 8 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 2 0.0 3 0 0 0 2 83 4 0.0 3 0 0 0 4 84 4 0.0 3 0 0 0 4 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 1 1 87 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 1 141 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 217 5 0.0 3 0 0 0 5 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 256 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 2 0.0 3 0 0 0 2 281 17 0.0 3 0 0 1 16 282 5 0.0 3 0 0 0 5 283 3 0.0 3 0 0 0 3 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 292 2 0.0 3 0 0 0 2 294 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.40 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 97,366 Reads with adapters: 310 (0.3%) Reads written (passing filters): 97,366 (100.0%) Total basepairs processed: 26,963,372 bp Total written (filtered): 26,962,405 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 310 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.5% C: 58.4% G: 20.3% T: 5.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 293 1521.3 0 293 4 9 380.3 0 9 5 2 95.1 0 2 6 5 23.8 0 5 12 1 0.0 1 0 1