This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.64 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 211,632 (8.3%) Reads that were too short: 7,504 (0.3%) Reads written (passing filters): 204,128 (8.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 58,791,692 bp (7.7%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 211632 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1837 621.6 0 1837 7 92615 621.6 0 92615 8 99804 621.6 0 99804 9 73 621.6 0 73 10 50 621.6 0 50 11 30 621.6 0 30 12 28 621.6 0 28 13 44 621.6 0 44 14 23 621.6 0 23 15 28 621.6 0 28 16 43 621.6 0 43 17 42 621.6 0 42 18 37 621.6 0 37 19 26 621.6 0 26 20 26 621.6 0 26 21 22 621.6 0 22 22 28 621.6 0 28 23 27 621.6 0 27 24 27 621.6 0 27 25 26 621.6 0 26 26 21 621.6 0 21 27 26 621.6 0 26 28 26 621.6 0 26 29 24 621.6 0 24 30 22 621.6 0 22 31 33 621.6 0 33 32 22 621.6 0 22 33 11 621.6 0 11 34 27 621.6 0 27 35 30 621.6 0 30 36 28 621.6 0 28 37 30 621.6 0 30 38 29 621.6 0 29 39 35 621.6 0 35 40 32 621.6 0 32 41 31 621.6 0 31 42 86 621.6 0 86 43 21 621.6 0 21 44 24 621.6 0 24 45 34 621.6 0 34 46 26 621.6 0 26 47 22 621.6 0 22 48 21 621.6 0 21 49 27 621.6 0 27 50 28 621.6 0 28 51 29 621.6 0 29 52 15 621.6 0 15 53 17 621.6 0 17 54 20 621.6 0 20 55 25 621.6 0 25 56 32 621.6 0 32 57 27 621.6 0 27 58 27 621.6 0 27 59 35 621.6 0 35 60 25 621.6 0 25 61 23 621.6 0 23 62 29 621.6 0 29 63 49 621.6 0 49 64 25 621.6 0 25 65 19 621.6 0 19 66 26 621.6 0 26 67 29 621.6 0 29 68 23 621.6 0 23 69 30 621.6 0 30 70 23 621.6 0 23 71 20 621.6 0 20 72 24 621.6 0 24 73 33 621.6 0 33 74 19 621.6 0 19 75 45 621.6 0 45 76 28 621.6 0 28 77 42 621.6 0 42 78 24 621.6 0 24 79 18 621.6 0 18 80 38 621.6 0 38 81 38 621.6 0 38 82 36 621.6 0 36 83 54 621.6 0 54 84 32 621.6 0 32 85 37 621.6 0 37 86 43 621.6 0 43 87 60 621.6 0 60 88 20 621.6 0 20 89 43 621.6 0 43 90 49 621.6 0 49 91 60 621.6 0 60 92 51 621.6 0 51 93 40 621.6 0 40 94 42 621.6 0 42 95 42 621.6 0 42 96 55 621.6 0 55 97 56 621.6 0 56 98 60 621.6 0 60 99 66 621.6 0 66 100 55 621.6 0 55 101 50 621.6 0 50 102 51 621.6 0 51 103 42 621.6 0 42 104 45 621.6 0 45 105 49 621.6 0 49 106 82 621.6 0 82 107 68 621.6 0 68 108 152 621.6 0 152 109 139 621.6 0 139 110 204 621.6 0 204 111 68 621.6 0 68 112 69 621.6 0 69 113 70 621.6 0 70 114 91 621.6 0 91 115 93 621.6 0 93 116 126 621.6 0 126 117 70 621.6 0 70 118 46 621.6 0 46 119 51 621.6 0 51 120 55 621.6 0 55 121 42 621.6 0 42 122 59 621.6 0 59 123 67 621.6 0 67 124 55 621.6 0 55 125 44 621.6 0 44 126 49 621.6 0 49 127 47 621.6 0 47 128 64 621.6 0 64 129 74 621.6 0 74 130 64 621.6 0 64 131 79 621.6 0 79 132 65 621.6 0 65 133 53 621.6 0 53 134 64 621.6 0 64 135 71 621.6 0 71 136 87 621.6 0 87 137 65 621.6 0 65 138 72 621.6 0 72 139 63 621.6 0 63 140 60 621.6 0 60 141 80 621.6 0 80 142 83 621.6 0 83 143 59 621.6 0 59 144 69 621.6 0 69 145 75 621.6 0 75 146 51 621.6 0 51 147 48 621.6 0 48 148 51 621.6 0 51 149 69 621.6 0 69 150 56 621.6 0 56 151 60 621.6 0 60 152 47 621.6 0 47 153 62 621.6 0 62 154 52 621.6 0 52 155 94 621.6 0 94 156 63 621.6 0 63 157 111 621.6 0 111 158 70 621.6 0 70 159 82 621.6 0 82 160 51 621.6 0 51 161 44 621.6 0 44 162 82 621.6 0 82 163 72 621.6 0 72 164 100 621.6 0 100 165 87 621.6 0 87 166 67 621.6 0 67 167 74 621.6 0 74 168 49 621.6 0 49 169 43 621.6 0 43 170 78 621.6 0 78 171 52 621.6 0 52 172 57 621.6 0 57 173 49 621.6 0 49 174 51 621.6 0 51 175 57 621.6 0 57 176 71 621.6 0 71 177 50 621.6 0 50 178 104 621.6 0 104 179 63 621.6 0 63 180 70 621.6 0 70 181 61 621.6 0 61 182 77 621.6 0 77 183 72 621.6 0 72 184 66 621.6 0 66 185 50 621.6 0 50 186 52 621.6 0 52 187 60 621.6 0 60 188 66 621.6 0 66 189 57 621.6 0 57 190 87 621.6 0 87 191 62 621.6 0 62 192 55 621.6 0 55 193 50 621.6 0 50 194 55 621.6 0 55 195 68 621.6 0 68 196 43 621.6 0 43 197 51 621.6 0 51 198 55 621.6 0 55 199 52 621.6 0 52 200 72 621.6 0 72 201 89 621.6 0 89 202 95 621.6 0 95 203 59 621.6 0 59 204 65 621.6 0 65 205 52 621.6 0 52 206 53 621.6 0 53 207 63 621.6 0 63 208 87 621.6 0 87 209 92 621.6 0 92 210 81 621.6 0 81 211 104 621.6 0 104 212 67 621.6 0 67 213 60 621.6 0 60 214 51 621.6 0 51 215 48 621.6 0 48 216 68 621.6 0 68 217 53 621.6 0 53 218 60 621.6 0 60 219 62 621.6 0 62 220 57 621.6 0 57 221 63 621.6 0 63 222 77 621.6 0 77 223 79 621.6 0 79 224 90 621.6 0 90 225 51 621.6 0 51 226 62 621.6 0 62 227 51 621.6 0 51 228 42 621.6 0 42 229 60 621.6 0 60 230 45 621.6 0 45 231 47 621.6 0 47 232 54 621.6 0 54 233 51 621.6 0 51 234 40 621.6 0 40 235 53 621.6 0 53 236 61 621.6 0 61 237 72 621.6 0 72 238 57 621.6 0 57 239 47 621.6 0 47 240 54 621.6 0 54 241 53 621.6 0 53 242 66 621.6 0 66 243 143 621.6 0 143 244 125 621.6 0 125 245 85 621.6 0 85 246 111 621.6 0 111 247 56 621.6 0 56 248 56 621.6 0 56 249 49 621.6 0 49 250 52 621.6 0 52 251 68 621.6 0 68 252 112 621.6 0 112 253 224 621.6 0 224 254 183 621.6 0 183 255 309 621.6 0 309 256 50 621.6 0 50 257 42 621.6 0 42 258 48 621.6 0 48 259 76 621.6 0 76 260 78 621.6 0 78 261 59 621.6 0 59 262 75 621.6 0 75 263 146 621.6 0 146 264 117 621.6 0 117 265 131 621.6 0 131 266 54 621.6 0 54 267 46 621.6 0 46 268 34 621.6 0 34 269 58 621.6 0 58 270 60 621.6 0 60 271 132 621.6 0 132 272 77 621.6 0 77 273 97 621.6 0 97 274 56 621.6 0 56 275 53 621.6 0 53 276 67 621.6 0 67 277 70 621.6 0 70 278 46 621.6 0 46 279 44 621.6 0 44 280 60 621.6 0 60 281 77 621.6 0 77 282 71 621.6 0 71 283 84 621.6 0 84 284 90 621.6 0 90 285 97 621.6 0 97 286 53 621.6 0 53 287 63 621.6 0 63 288 51 621.6 0 51 289 77 621.6 0 77 290 79 621.6 0 79 291 132 621.6 0 132 292 143 621.6 0 143 293 46 621.6 0 46 294 60 621.6 0 60 295 54 621.6 0 54 296 73 621.6 0 73 297 66 621.6 0 66 298 82 621.6 0 82 299 60 621.6 0 60 300 78 621.6 0 78 301 67 621.6 0 67 Finished in 10.07 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 204,128 Reads with adapters: 102,281 (50.1%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 102,261 (50.1%) Total basepairs processed: 58,791,692 bp Total written (filtered): 27,896,254 bp (47.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 102281 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 26 0.0 3 0 0 23 2 1 19 651 0.0 3 0 633 14 3 1 20 100982 0.0 4 99506 1422 45 8 1 21 273 0.0 4 41 226 5 1 22 21 0.0 4 8 1 11 1 23 82 0.0 4 1 0 0 76 5 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 1 26 3 0.0 4 0 0 0 0 3 33 1 0.0 4 0 0 1 38 1 0.0 4 1 44 1 0.0 4 1 48 2 0.0 4 0 0 0 1 1 67 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 211 0.0 4 0 0 0 0 211 129 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 1 171 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 1 208 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 1 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 1 282 5 0.0 4 0 0 0 0 5 283 1 0.0 4 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.63 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 102,261 Reads with adapters: 10,695 (10.5%) Reads written (passing filters): 102,261 (100.0%) Total basepairs processed: 27,896,254 bp Total written (filtered): 27,780,101 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10695 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 3.1% G: 94.0% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 58 1597.8 0 58 4 20 399.5 0 20 5 7 99.9 0 7 6 5 25.0 0 5 7 1 6.2 0 1 9 106 0.4 0 5 101 10 3390 0.1 1 92 3298 11 7059 0.0 1 51 7008 12 13 0.0 1 0 13 13 11 0.0 1 1 10 14 2 0.0 1 0 2 15 3 0.0 1 0 3 19 1 0.0 1 0 1 41 1 0.0 1 0 1 70 1 0.0 1 1 75 1 0.0 1 1 79 1 0.0 1 1 105 1 0.0 1 1 148 1 0.0 1 1 151 5 0.0 1 0 5 159 1 0.0 1 0 1 170 2 0.0 1 1 1 209 2 0.0 1 2 227 1 0.0 1 1 258 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.