This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.83 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 231,429 (9.1%) Reads that were too short: 35,772 (1.4%) Reads written (passing filters): 195,657 (7.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 54,381,025 bp (7.1%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 231429 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6267 621.6 0 6267 7 40521 621.6 0 40521 8 5183 621.6 0 5183 9 115626 621.6 0 115626 10 184 621.6 0 184 11 88 621.6 0 88 12 69 621.6 0 69 13 87 621.6 0 87 14 93 621.6 0 93 15 74 621.6 0 74 16 70 621.6 0 70 17 118 621.6 0 118 18 173 621.6 0 173 19 172 621.6 0 172 20 82 621.6 0 82 21 93 621.6 0 93 22 69 621.6 0 69 23 81 621.6 0 81 24 72 621.6 0 72 25 78 621.6 0 78 26 67 621.6 0 67 27 86 621.6 0 86 28 57 621.6 0 57 29 81 621.6 0 81 30 69 621.6 0 69 31 81 621.6 0 81 32 75 621.6 0 75 33 81 621.6 0 81 34 84 621.6 0 84 35 62 621.6 0 62 36 59 621.6 0 59 37 83 621.6 0 83 38 78 621.6 0 78 39 104 621.6 0 104 40 105 621.6 0 105 41 79 621.6 0 79 42 112 621.6 0 112 43 93 621.6 0 93 44 97 621.6 0 97 45 84 621.6 0 84 46 112 621.6 0 112 47 595 621.6 0 595 48 441 621.6 0 441 49 604 621.6 0 604 50 164 621.6 0 164 51 73 621.6 0 73 52 87 621.6 0 87 53 80 621.6 0 80 54 65 621.6 0 65 55 93 621.6 0 93 56 93 621.6 0 93 57 86 621.6 0 86 58 86 621.6 0 86 59 173 621.6 0 173 60 166 621.6 0 166 61 199 621.6 0 199 62 105 621.6 0 105 63 90 621.6 0 90 64 77 621.6 0 77 65 86 621.6 0 86 66 66 621.6 0 66 67 71 621.6 0 71 68 73 621.6 0 73 69 103 621.6 0 103 70 95 621.6 0 95 71 96 621.6 0 96 72 132 621.6 0 132 73 69 621.6 0 69 74 83 621.6 0 83 75 70 621.6 0 70 76 122 621.6 0 122 77 294 621.6 0 294 78 261 621.6 0 261 79 156 621.6 0 156 80 78 621.6 0 78 81 87 621.6 0 87 82 102 621.6 0 102 83 106 621.6 0 106 84 537 621.6 0 537 85 294 621.6 0 294 86 421 621.6 0 421 87 62 621.6 0 62 88 64 621.6 0 64 89 90 621.6 0 90 90 51 621.6 0 51 91 95 621.6 0 95 92 83 621.6 0 83 93 71 621.6 0 71 94 82 621.6 0 82 95 75 621.6 0 75 96 47 621.6 0 47 97 96 621.6 0 96 98 53 621.6 0 53 99 60 621.6 0 60 100 61 621.6 0 61 101 75 621.6 0 75 102 57 621.6 0 57 103 53 621.6 0 53 104 59 621.6 0 59 105 60 621.6 0 60 106 75 621.6 0 75 107 69 621.6 0 69 108 67 621.6 0 67 109 66 621.6 0 66 110 62 621.6 0 62 111 100 621.6 0 100 112 251 621.6 0 251 113 258 621.6 0 258 114 279 621.6 0 279 115 147 621.6 0 147 116 76 621.6 0 76 117 207 621.6 0 207 118 113 621.6 0 113 119 88 621.6 0 88 120 64 621.6 0 64 121 63 621.6 0 63 122 71 621.6 0 71 123 94 621.6 0 94 124 103 621.6 0 103 125 227 621.6 0 227 126 87 621.6 0 87 127 154 621.6 0 154 128 191 621.6 0 191 129 183 621.6 0 183 130 208 621.6 0 208 131 167 621.6 0 167 132 342 621.6 0 342 133 351 621.6 0 351 134 527 621.6 0 527 135 279 621.6 0 279 136 176 621.6 0 176 137 160 621.6 0 160 138 150 621.6 0 150 139 190 621.6 0 190 140 224 621.6 0 224 141 2447 621.6 0 2447 142 488 621.6 0 488 143 1897 621.6 0 1897 144 99 621.6 0 99 145 70 621.6 0 70 146 97 621.6 0 97 147 88 621.6 0 88 148 103 621.6 0 103 149 100 621.6 0 100 150 81 621.6 0 81 151 108 621.6 0 108 152 343 621.6 0 343 153 413 621.6 0 413 154 377 621.6 0 377 155 177 621.6 0 177 156 76 621.6 0 76 157 59 621.6 0 59 158 76 621.6 0 76 159 87 621.6 0 87 160 53 621.6 0 53 161 51 621.6 0 51 162 82 621.6 0 82 163 69 621.6 0 69 164 72 621.6 0 72 165 78 621.6 0 78 166 76 621.6 0 76 167 64 621.6 0 64 168 74 621.6 0 74 169 90 621.6 0 90 170 62 621.6 0 62 171 74 621.6 0 74 172 87 621.6 0 87 173 121 621.6 0 121 174 75 621.6 0 75 175 84 621.6 0 84 176 82 621.6 0 82 177 57 621.6 0 57 178 60 621.6 0 60 179 82 621.6 0 82 180 74 621.6 0 74 181 76 621.6 0 76 182 88 621.6 0 88 183 82 621.6 0 82 184 105 621.6 0 105 185 93 621.6 0 93 186 100 621.6 0 100 187 141 621.6 0 141 188 97 621.6 0 97 189 110 621.6 0 110 190 120 621.6 0 120 191 116 621.6 0 116 192 117 621.6 0 117 193 139 621.6 0 139 194 75 621.6 0 75 195 109 621.6 0 109 196 87 621.6 0 87 197 87 621.6 0 87 198 82 621.6 0 82 199 101 621.6 0 101 200 85 621.6 0 85 201 103 621.6 0 103 202 152 621.6 0 152 203 181 621.6 0 181 204 76 621.6 0 76 205 91 621.6 0 91 206 82 621.6 0 82 207 84 621.6 0 84 208 65 621.6 0 65 209 72 621.6 0 72 210 72 621.6 0 72 211 99 621.6 0 99 212 199 621.6 0 199 213 207 621.6 0 207 214 221 621.6 0 221 215 186 621.6 0 186 216 120 621.6 0 120 217 192 621.6 0 192 218 237 621.6 0 237 219 224 621.6 0 224 220 203 621.6 0 203 221 101 621.6 0 101 222 89 621.6 0 89 223 60 621.6 0 60 224 65 621.6 0 65 225 127 621.6 0 127 226 140 621.6 0 140 227 119 621.6 0 119 228 191 621.6 0 191 229 152 621.6 0 152 230 227 621.6 0 227 231 179 621.6 0 179 232 217 621.6 0 217 233 111 621.6 0 111 234 184 621.6 0 184 235 851 621.6 0 851 236 635 621.6 0 635 237 1087 621.6 0 1087 238 601 621.6 0 601 239 591 621.6 0 591 240 254 621.6 0 254 241 150 621.6 0 150 242 103 621.6 0 103 243 183 621.6 0 183 244 161 621.6 0 161 245 365 621.6 0 365 246 304 621.6 0 304 247 358 621.6 0 358 248 253 621.6 0 253 249 168 621.6 0 168 250 191 621.6 0 191 251 168 621.6 0 168 252 886 621.6 0 886 253 463 621.6 0 463 254 537 621.6 0 537 255 437 621.6 0 437 256 921 621.6 0 921 257 796 621.6 0 796 258 417 621.6 0 417 259 139 621.6 0 139 260 226 621.6 0 226 261 208 621.6 0 208 262 152 621.6 0 152 263 117 621.6 0 117 264 114 621.6 0 114 265 102 621.6 0 102 266 125 621.6 0 125 267 82 621.6 0 82 268 115 621.6 0 115 269 98 621.6 0 98 270 98 621.6 0 98 271 144 621.6 0 144 272 348 621.6 0 348 273 507 621.6 0 507 274 353 621.6 0 353 275 367 621.6 0 367 276 102 621.6 0 102 277 85 621.6 0 85 278 118 621.6 0 118 279 128 621.6 0 128 280 185 621.6 0 185 281 141 621.6 0 141 282 212 621.6 0 212 283 361 621.6 0 361 284 472 621.6 0 472 285 576 621.6 0 576 286 474 621.6 0 474 287 562 621.6 0 562 288 313 621.6 0 313 289 209 621.6 0 209 290 241 621.6 0 241 291 246 621.6 0 246 292 239 621.6 0 239 293 302 621.6 0 302 294 241 621.6 0 241 295 263 621.6 0 263 296 458 621.6 0 458 297 550 621.6 0 550 298 2797 621.6 0 2797 299 3071 621.6 0 3071 300 3566 621.6 0 3566 301 960 621.6 0 960 Finished in 9.09 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 195,657 Reads with adapters: 50,764 (25.9%) Reads that were too short: 215 (0.1%) Reads written (passing filters): 50,549 (25.8%) Total basepairs processed: 54,381,025 bp Total written (filtered): 13,834,905 bp (25.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50764 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 29 0.0 3 0 0 15 14 16 384 0.0 3 0 317 32 35 17 48318 0.0 3 44361 3203 597 157 18 115 0.0 3 14 72 18 11 19 1184 0.0 3 993 50 67 74 20 47 0.0 3 0 0 1 46 21 6 0.0 3 0 1 2 3 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 27 1 0.0 3 1 33 1 0.0 3 0 0 1 34 1 0.0 3 0 0 0 1 36 3 0.0 3 0 0 1 2 37 1 0.0 3 0 0 0 1 38 3 0.0 3 0 0 0 3 39 60 0.0 3 0 0 0 60 41 2 0.0 3 0 0 0 2 42 16 0.0 3 0 0 2 14 43 3 0.0 3 0 0 0 3 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 12 0.0 3 0 0 0 12 49 4 0.0 3 0 0 0 4 50 114 0.0 3 0 0 0 114 51 5 0.0 3 0 0 0 5 53 15 0.0 3 0 0 1 14 54 2 0.0 3 0 0 1 1 55 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 65 5 0.0 3 0 0 0 5 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 79 16 0.0 3 0 0 0 16 80 1 0.0 3 0 0 0 1 82 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 0 1 87 14 0.0 3 0 0 3 11 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 7 0.0 3 0 0 0 7 91 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 93 2 0.0 3 0 0 0 2 94 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 5 0.0 3 0 0 0 5 100 2 0.0 3 0 0 0 2 101 46 0.0 3 0 0 0 46 102 6 0.0 3 0 0 0 6 103 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 6 0.0 3 0 0 0 6 108 3 0.0 3 0 0 0 3 109 3 0.0 3 0 0 0 3 110 3 0.0 3 0 0 0 3 111 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 7 0.0 3 0 0 0 7 122 1 0.0 3 0 0 0 1 123 7 0.0 3 0 0 1 6 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 6 0.0 3 0 0 0 6 133 5 0.0 3 0 0 0 5 138 1 0.0 3 0 0 0 1 139 3 0.0 3 0 0 0 3 140 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 25 0.0 3 0 0 1 24 144 4 0.0 3 0 0 0 4 145 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 8 0.0 3 0 0 0 8 152 1 0.0 3 0 0 0 1 154 4 0.0 3 0 0 0 4 156 2 0.0 3 0 0 0 2 157 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 166 3 0.0 3 0 0 0 3 169 4 0.0 3 0 0 1 3 172 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 190 6 0.0 3 0 0 0 6 191 2 0.0 3 0 0 0 2 192 20 0.0 3 0 0 1 19 193 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 195 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 3 0.0 3 0 0 0 3 203 4 0.0 3 0 0 0 4 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 207 2 0.0 3 0 0 0 2 208 4 0.0 3 0 0 0 4 209 1 0.0 3 0 0 1 210 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 1 1 214 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 219 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 1 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 268 6 0.0 3 0 0 0 6 269 3 0.0 3 0 0 0 3 271 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 5 0.0 3 0 0 0 5 278 6 0.0 3 0 0 0 6 279 19 0.0 3 0 0 1 18 280 12 0.0 3 0 0 0 12 281 8 0.0 3 0 0 0 8 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 286 3 0.0 3 0 0 0 3 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 290 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.79 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 50,549 Reads with adapters: 298 (0.6%) Reads written (passing filters): 50,549 (100.0%) Total basepairs processed: 13,834,905 bp Total written (filtered): 13,833,906 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 298 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.2% C: 6.7% G: 64.1% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 205 789.8 0 205 4 88 197.5 0 88 5 2 49.4 0 2 6 2 12.3 0 2 10 1 0.0 1 0 1