This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.24 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 240,689 (9.5%) Reads that were too short: 16,835 (0.7%) Reads written (passing filters): 223,854 (8.8%) Total basepairs processed: 766,381,518 bp Total written (filtered): 62,861,093 bp (8.2%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 240689 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6480 621.6 0 6480 7 50431 621.6 0 50431 8 5670 621.6 0 5670 9 135762 621.6 0 135762 10 186 621.6 0 186 11 104 621.6 0 104 12 102 621.6 0 102 13 126 621.6 0 126 14 116 621.6 0 116 15 88 621.6 0 88 16 76 621.6 0 76 17 101 621.6 0 101 18 115 621.6 0 115 19 94 621.6 0 94 20 86 621.6 0 86 21 104 621.6 0 104 22 95 621.6 0 95 23 83 621.6 0 83 24 99 621.6 0 99 25 75 621.6 0 75 26 84 621.6 0 84 27 86 621.6 0 86 28 81 621.6 0 81 29 94 621.6 0 94 30 83 621.6 0 83 31 71 621.6 0 71 32 75 621.6 0 75 33 96 621.6 0 96 34 114 621.6 0 114 35 88 621.6 0 88 36 101 621.6 0 101 37 112 621.6 0 112 38 95 621.6 0 95 39 86 621.6 0 86 40 90 621.6 0 90 41 88 621.6 0 88 42 109 621.6 0 109 43 75 621.6 0 75 44 100 621.6 0 100 45 97 621.6 0 97 46 126 621.6 0 126 47 154 621.6 0 154 48 163 621.6 0 163 49 176 621.6 0 176 50 131 621.6 0 131 51 88 621.6 0 88 52 111 621.6 0 111 53 101 621.6 0 101 54 145 621.6 0 145 55 122 621.6 0 122 56 100 621.6 0 100 57 94 621.6 0 94 58 113 621.6 0 113 59 146 621.6 0 146 60 120 621.6 0 120 61 158 621.6 0 158 62 109 621.6 0 109 63 99 621.6 0 99 64 67 621.6 0 67 65 78 621.6 0 78 66 98 621.6 0 98 67 80 621.6 0 80 68 105 621.6 0 105 69 124 621.6 0 124 70 91 621.6 0 91 71 98 621.6 0 98 72 112 621.6 0 112 73 102 621.6 0 102 74 82 621.6 0 82 75 93 621.6 0 93 76 114 621.6 0 114 77 158 621.6 0 158 78 134 621.6 0 134 79 114 621.6 0 114 80 86 621.6 0 86 81 104 621.6 0 104 82 90 621.6 0 90 83 113 621.6 0 113 84 259 621.6 0 259 85 179 621.6 0 179 86 243 621.6 0 243 87 76 621.6 0 76 88 79 621.6 0 79 89 79 621.6 0 79 90 78 621.6 0 78 91 105 621.6 0 105 92 87 621.6 0 87 93 93 621.6 0 93 94 114 621.6 0 114 95 93 621.6 0 93 96 88 621.6 0 88 97 60 621.6 0 60 98 72 621.6 0 72 99 86 621.6 0 86 100 73 621.6 0 73 101 76 621.6 0 76 102 99 621.6 0 99 103 79 621.6 0 79 104 60 621.6 0 60 105 81 621.6 0 81 106 108 621.6 0 108 107 93 621.6 0 93 108 103 621.6 0 103 109 91 621.6 0 91 110 109 621.6 0 109 111 75 621.6 0 75 112 272 621.6 0 272 113 315 621.6 0 315 114 292 621.6 0 292 115 165 621.6 0 165 116 89 621.6 0 89 117 107 621.6 0 107 118 105 621.6 0 105 119 79 621.6 0 79 120 96 621.6 0 96 121 89 621.6 0 89 122 79 621.6 0 79 123 94 621.6 0 94 124 81 621.6 0 81 125 112 621.6 0 112 126 96 621.6 0 96 127 144 621.6 0 144 128 161 621.6 0 161 129 143 621.6 0 143 130 163 621.6 0 163 131 130 621.6 0 130 132 212 621.6 0 212 133 224 621.6 0 224 134 301 621.6 0 301 135 222 621.6 0 222 136 179 621.6 0 179 137 150 621.6 0 150 138 104 621.6 0 104 139 117 621.6 0 117 140 182 621.6 0 182 141 2209 621.6 0 2209 142 414 621.6 0 414 143 1682 621.6 0 1682 144 85 621.6 0 85 145 79 621.6 0 79 146 82 621.6 0 82 147 103 621.6 0 103 148 103 621.6 0 103 149 101 621.6 0 101 150 84 621.6 0 84 151 122 621.6 0 122 152 190 621.6 0 190 153 254 621.6 0 254 154 259 621.6 0 259 155 142 621.6 0 142 156 99 621.6 0 99 157 100 621.6 0 100 158 91 621.6 0 91 159 100 621.6 0 100 160 90 621.6 0 90 161 92 621.6 0 92 162 100 621.6 0 100 163 90 621.6 0 90 164 104 621.6 0 104 165 118 621.6 0 118 166 110 621.6 0 110 167 85 621.6 0 85 168 81 621.6 0 81 169 103 621.6 0 103 170 83 621.6 0 83 171 87 621.6 0 87 172 89 621.6 0 89 173 126 621.6 0 126 174 87 621.6 0 87 175 105 621.6 0 105 176 84 621.6 0 84 177 55 621.6 0 55 178 91 621.6 0 91 179 82 621.6 0 82 180 87 621.6 0 87 181 81 621.6 0 81 182 87 621.6 0 87 183 88 621.6 0 88 184 145 621.6 0 145 185 95 621.6 0 95 186 146 621.6 0 146 187 167 621.6 0 167 188 86 621.6 0 86 189 82 621.6 0 82 190 104 621.6 0 104 191 105 621.6 0 105 192 96 621.6 0 96 193 113 621.6 0 113 194 68 621.6 0 68 195 97 621.6 0 97 196 109 621.6 0 109 197 76 621.6 0 76 198 76 621.6 0 76 199 106 621.6 0 106 200 83 621.6 0 83 201 138 621.6 0 138 202 221 621.6 0 221 203 204 621.6 0 204 204 84 621.6 0 84 205 90 621.6 0 90 206 86 621.6 0 86 207 88 621.6 0 88 208 53 621.6 0 53 209 78 621.6 0 78 210 66 621.6 0 66 211 90 621.6 0 90 212 98 621.6 0 98 213 133 621.6 0 133 214 126 621.6 0 126 215 99 621.6 0 99 216 86 621.6 0 86 217 105 621.6 0 105 218 89 621.6 0 89 219 91 621.6 0 91 220 83 621.6 0 83 221 74 621.6 0 74 222 55 621.6 0 55 223 63 621.6 0 63 224 74 621.6 0 74 225 94 621.6 0 94 226 131 621.6 0 131 227 92 621.6 0 92 228 183 621.6 0 183 229 91 621.6 0 91 230 116 621.6 0 116 231 96 621.6 0 96 232 107 621.6 0 107 233 82 621.6 0 82 234 85 621.6 0 85 235 301 621.6 0 301 236 221 621.6 0 221 237 363 621.6 0 363 238 144 621.6 0 144 239 140 621.6 0 140 240 80 621.6 0 80 241 67 621.6 0 67 242 68 621.6 0 68 243 91 621.6 0 91 244 74 621.6 0 74 245 105 621.6 0 105 246 116 621.6 0 116 247 99 621.6 0 99 248 115 621.6 0 115 249 69 621.6 0 69 250 88 621.6 0 88 251 68 621.6 0 68 252 178 621.6 0 178 253 111 621.6 0 111 254 96 621.6 0 96 255 188 621.6 0 188 256 356 621.6 0 356 257 325 621.6 0 325 258 172 621.6 0 172 259 57 621.6 0 57 260 134 621.6 0 134 261 195 621.6 0 195 262 147 621.6 0 147 263 79 621.6 0 79 264 80 621.6 0 80 265 75 621.6 0 75 266 63 621.6 0 63 267 76 621.6 0 76 268 84 621.6 0 84 269 63 621.6 0 63 270 93 621.6 0 93 271 72 621.6 0 72 272 125 621.6 0 125 273 462 621.6 0 462 274 306 621.6 0 306 275 427 621.6 0 427 276 102 621.6 0 102 277 42 621.6 0 42 278 73 621.6 0 73 279 80 621.6 0 80 280 88 621.6 0 88 281 69 621.6 0 69 282 115 621.6 0 115 283 124 621.6 0 124 284 131 621.6 0 131 285 175 621.6 0 175 286 145 621.6 0 145 287 225 621.6 0 225 288 161 621.6 0 161 289 108 621.6 0 108 290 97 621.6 0 97 291 109 621.6 0 109 292 124 621.6 0 124 293 126 621.6 0 126 294 132 621.6 0 132 295 135 621.6 0 135 296 153 621.6 0 153 297 221 621.6 0 221 298 1188 621.6 0 1188 299 1275 621.6 0 1275 300 1897 621.6 0 1897 301 254 621.6 0 254 Finished in 11.26 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 223,854 Reads with adapters: 50,909 (22.7%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 50,896 (22.7%) Total basepairs processed: 62,861,093 bp Total written (filtered): 13,882,223 bp (22.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 50909 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 22 0.0 3 0 0 21 0 1 19 577 0.0 3 0 558 19 20 48013 0.0 4 46907 1060 37 8 1 21 190 0.0 4 48 140 1 1 22 1432 0.0 4 1406 18 6 1 1 23 63 0.0 4 3 2 0 48 10 24 3 0.0 4 0 0 0 1 2 25 2 0.0 4 1 0 0 1 28 1 0.0 4 0 0 0 1 31 1 0.0 4 1 36 1 0.0 4 0 1 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 1 1 123 1 0.0 4 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 7 0.0 4 0 0 0 0 7 127 568 0.0 4 0 0 0 0 568 129 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 151 1 0.0 4 1 153 1 0.0 4 1 169 1 0.0 4 0 0 0 0 1 195 1 0.0 4 1 207 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.78 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 50,896 Reads with adapters: 3,072 (6.0%) Reads written (passing filters): 50,896 (100.0%) Total basepairs processed: 13,882,223 bp Total written (filtered): 13,848,529 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3072 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.1% C: 2.7% G: 92.9% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 112 795.2 0 112 4 21 198.8 0 21 5 9 49.7 0 9 7 1 3.1 0 1 9 153 0.2 0 5 148 10 101 0.0 1 3 98 11 2489 0.0 1 12 2477 12 169 0.0 1 6 163 13 7 0.0 1 1 6 14 1 0.0 1 1 15 2 0.0 1 0 2 152 4 0.0 1 0 4 171 1 0.0 1 1 257 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.