This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.82 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 353,515 (13.9%) Reads that were too short: 48,648 (1.9%) Reads written (passing filters): 304,867 (12.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 87,633,857 bp (11.4%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 353515 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4313 621.6 0 4313 7 152005 621.6 0 152005 8 2980 621.6 0 2980 9 131808 621.6 0 131808 10 268 621.6 0 268 11 57 621.6 0 57 12 51 621.6 0 51 13 27 621.6 0 27 14 25 621.6 0 25 15 31 621.6 0 31 16 40 621.6 0 40 17 18 621.6 0 18 18 20 621.6 0 20 19 25 621.6 0 25 20 19 621.6 0 19 21 29 621.6 0 29 22 20 621.6 0 20 23 25 621.6 0 25 24 32 621.6 0 32 25 27 621.6 0 27 26 27 621.6 0 27 27 23 621.6 0 23 28 21 621.6 0 21 29 21 621.6 0 21 30 33 621.6 0 33 31 24 621.6 0 24 32 35 621.6 0 35 33 29 621.6 0 29 34 17 621.6 0 17 35 18 621.6 0 18 36 16 621.6 0 16 37 22 621.6 0 22 38 17 621.6 0 17 39 19 621.6 0 19 40 25 621.6 0 25 41 33 621.6 0 33 42 36 621.6 0 36 43 23 621.6 0 23 44 27 621.6 0 27 45 25 621.6 0 25 46 22 621.6 0 22 47 16 621.6 0 16 48 20 621.6 0 20 49 17 621.6 0 17 50 25 621.6 0 25 51 9 621.6 0 9 52 23 621.6 0 23 53 36 621.6 0 36 54 34 621.6 0 34 55 79 621.6 0 79 56 56 621.6 0 56 57 44 621.6 0 44 58 23 621.6 0 23 59 36 621.6 0 36 60 24 621.6 0 24 61 50 621.6 0 50 62 38 621.6 0 38 63 30 621.6 0 30 64 30 621.6 0 30 65 26 621.6 0 26 66 20 621.6 0 20 67 45 621.6 0 45 68 30 621.6 0 30 69 48 621.6 0 48 70 163 621.6 0 163 71 44 621.6 0 44 72 48 621.6 0 48 73 55 621.6 0 55 74 109 621.6 0 109 75 78 621.6 0 78 76 108 621.6 0 108 77 33 621.6 0 33 78 31 621.6 0 31 79 54 621.6 0 54 80 53 621.6 0 53 81 146 621.6 0 146 82 249 621.6 0 249 83 209 621.6 0 209 84 363 621.6 0 363 85 96 621.6 0 96 86 99 621.6 0 99 87 42 621.6 0 42 88 40 621.6 0 40 89 50 621.6 0 50 90 35 621.6 0 35 91 45 621.6 0 45 92 44 621.6 0 44 93 31 621.6 0 31 94 34 621.6 0 34 95 30 621.6 0 30 96 28 621.6 0 28 97 29 621.6 0 29 98 32 621.6 0 32 99 20 621.6 0 20 100 33 621.6 0 33 101 27 621.6 0 27 102 28 621.6 0 28 103 34 621.6 0 34 104 51 621.6 0 51 105 37 621.6 0 37 106 41 621.6 0 41 107 35 621.6 0 35 108 32 621.6 0 32 109 33 621.6 0 33 110 41 621.6 0 41 111 33 621.6 0 33 112 57 621.6 0 57 113 54 621.6 0 54 114 38 621.6 0 38 115 37 621.6 0 37 116 45 621.6 0 45 117 26 621.6 0 26 118 26 621.6 0 26 119 49 621.6 0 49 120 31 621.6 0 31 121 68 621.6 0 68 122 113 621.6 0 113 123 345 621.6 0 345 124 56 621.6 0 56 125 83 621.6 0 83 126 45 621.6 0 45 127 56 621.6 0 56 128 45 621.6 0 45 129 35 621.6 0 35 130 30 621.6 0 30 131 26 621.6 0 26 132 41 621.6 0 41 133 72 621.6 0 72 134 42 621.6 0 42 135 45 621.6 0 45 136 67 621.6 0 67 137 43 621.6 0 43 138 29 621.6 0 29 139 60 621.6 0 60 140 56 621.6 0 56 141 36 621.6 0 36 142 31 621.6 0 31 143 37 621.6 0 37 144 27 621.6 0 27 145 47 621.6 0 47 146 41 621.6 0 41 147 50 621.6 0 50 148 54 621.6 0 54 149 33 621.6 0 33 150 45 621.6 0 45 151 40 621.6 0 40 152 60 621.6 0 60 153 54 621.6 0 54 154 61 621.6 0 61 155 36 621.6 0 36 156 33 621.6 0 33 157 52 621.6 0 52 158 27 621.6 0 27 159 39 621.6 0 39 160 34 621.6 0 34 161 35 621.6 0 35 162 43 621.6 0 43 163 53 621.6 0 53 164 45 621.6 0 45 165 55 621.6 0 55 166 86 621.6 0 86 167 608 621.6 0 608 168 378 621.6 0 378 169 581 621.6 0 581 170 87 621.6 0 87 171 274 621.6 0 274 172 159 621.6 0 159 173 142 621.6 0 142 174 38 621.6 0 38 175 47 621.6 0 47 176 38 621.6 0 38 177 30 621.6 0 30 178 29 621.6 0 29 179 34 621.6 0 34 180 96 621.6 0 96 181 64 621.6 0 64 182 104 621.6 0 104 183 163 621.6 0 163 184 237 621.6 0 237 185 64 621.6 0 64 186 67 621.6 0 67 187 53 621.6 0 53 188 105 621.6 0 105 189 123 621.6 0 123 190 79 621.6 0 79 191 77 621.6 0 77 192 41 621.6 0 41 193 50 621.6 0 50 194 72 621.6 0 72 195 67 621.6 0 67 196 68 621.6 0 68 197 81 621.6 0 81 198 109 621.6 0 109 199 175 621.6 0 175 200 717 621.6 0 717 201 1021 621.6 0 1021 202 1100 621.6 0 1100 203 389 621.6 0 389 204 43 621.6 0 43 205 105 621.6 0 105 206 107 621.6 0 107 207 93 621.6 0 93 208 45 621.6 0 45 209 45 621.6 0 45 210 79 621.6 0 79 211 163 621.6 0 163 212 71 621.6 0 71 213 68 621.6 0 68 214 132 621.6 0 132 215 131 621.6 0 131 216 147 621.6 0 147 217 123 621.6 0 123 218 122 621.6 0 122 219 154 621.6 0 154 220 122 621.6 0 122 221 164 621.6 0 164 222 102 621.6 0 102 223 209 621.6 0 209 224 358 621.6 0 358 225 332 621.6 0 332 226 562 621.6 0 562 227 1037 621.6 0 1037 228 6349 621.6 0 6349 229 2474 621.6 0 2474 230 20156 621.6 0 20156 231 266 621.6 0 266 232 79 621.6 0 79 233 74 621.6 0 74 234 77 621.6 0 77 235 74 621.6 0 74 236 81 621.6 0 81 237 84 621.6 0 84 238 99 621.6 0 99 239 92 621.6 0 92 240 87 621.6 0 87 241 76 621.6 0 76 242 65 621.6 0 65 243 76 621.6 0 76 244 97 621.6 0 97 245 145 621.6 0 145 246 207 621.6 0 207 247 243 621.6 0 243 248 251 621.6 0 251 249 189 621.6 0 189 250 108 621.6 0 108 251 89 621.6 0 89 252 69 621.6 0 69 253 63 621.6 0 63 254 61 621.6 0 61 255 133 621.6 0 133 256 293 621.6 0 293 257 227 621.6 0 227 258 289 621.6 0 289 259 128 621.6 0 128 260 151 621.6 0 151 261 653 621.6 0 653 262 669 621.6 0 669 263 965 621.6 0 965 264 278 621.6 0 278 265 361 621.6 0 361 266 228 621.6 0 228 267 183 621.6 0 183 268 626 621.6 0 626 269 619 621.6 0 619 270 503 621.6 0 503 271 133 621.6 0 133 272 94 621.6 0 94 273 83 621.6 0 83 274 118 621.6 0 118 275 100 621.6 0 100 276 117 621.6 0 117 277 84 621.6 0 84 278 82 621.6 0 82 279 69 621.6 0 69 280 109 621.6 0 109 281 173 621.6 0 173 282 184 621.6 0 184 283 210 621.6 0 210 284 102 621.6 0 102 285 62 621.6 0 62 286 91 621.6 0 91 287 126 621.6 0 126 288 145 621.6 0 145 289 109 621.6 0 109 290 66 621.6 0 66 291 86 621.6 0 86 292 249 621.6 0 249 293 269 621.6 0 269 294 367 621.6 0 367 295 111 621.6 0 111 296 78 621.6 0 78 297 69 621.6 0 69 298 80 621.6 0 80 299 161 621.6 0 161 300 381 621.6 0 381 301 500 621.6 0 500 Finished in 13.59 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 304,867 Reads with adapters: 153,291 (50.3%) Reads that were too short: 140 (0.0%) Reads written (passing filters): 153,151 (50.2%) Total basepairs processed: 87,633,857 bp Total written (filtered): 42,412,190 bp (48.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 153291 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 40 0.0 2 0 0 0 40 15 94 0.0 3 0 0 60 34 16 1420 0.0 3 0 1164 147 109 17 150366 0.0 3 138267 9650 1953 496 18 320 0.0 3 42 232 26 20 19 650 0.0 3 13 1 349 287 20 155 0.0 3 0 1 1 153 21 2 0.0 3 1 0 0 1 22 1 0.0 3 0 0 0 1 23 5 0.0 3 4 0 0 1 24 5 0.0 3 0 0 0 5 25 3 0.0 3 1 0 1 1 26 2 0.0 3 2 27 1 0.0 3 1 28 3 0.0 3 2 1 29 1 0.0 3 0 0 1 31 1 0.0 3 1 33 1 0.0 3 1 34 3 0.0 3 1 0 0 2 38 3 0.0 3 0 0 0 3 39 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 48 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 9 0.0 3 0 0 0 9 73 3 0.0 3 0 0 0 3 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 1 0.0 3 1 137 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 164 8 0.0 3 0 0 0 8 165 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 1 170 1 0.0 3 0 1 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 4 0.0 3 0 0 0 4 184 4 0.0 3 0 0 0 4 185 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 190 5 0.0 3 0 0 0 5 192 2 0.0 3 0 0 0 2 194 9 0.0 3 0 0 0 9 195 4 0.0 3 0 0 0 4 200 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 221 4 0.0 3 0 0 0 4 223 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 1 1 232 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 2 0.0 3 0 0 0 2 237 5 0.0 3 0 0 0 5 238 3 0.0 3 0 0 0 3 244 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 1 278 5 0.0 3 0 0 0 5 279 8 0.0 3 0 0 0 8 280 10 0.0 3 0 0 0 10 281 16 0.0 3 0 0 0 16 282 12 0.0 3 0 0 0 12 283 2 0.0 3 0 0 0 2 285 2 0.0 3 0 0 0 2 286 2 0.0 3 0 0 0 2 288 3 0.0 3 0 0 0 3 289 2 0.0 3 0 0 0 2 290 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.33 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 153,151 Reads with adapters: 469 (0.3%) Reads written (passing filters): 153,151 (100.0%) Total basepairs processed: 42,412,190 bp Total written (filtered): 42,410,408 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 469 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.4% C: 49.5% G: 24.5% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 412 2393.0 0 412 4 13 598.2 0 13 5 1 149.6 0 1 6 4 37.4 0 4 9 1 0.6 0 0 1 12 38 0.0 1 0 38