This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.84 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 376,562 (14.8%) Reads that were too short: 57,155 (2.2%) Reads written (passing filters): 319,407 (12.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 91,892,326 bp (12.0%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 376562 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2154 621.6 0 2154 7 141067 621.6 0 141067 8 2263 621.6 0 2263 9 161448 621.6 0 161448 10 162 621.6 0 162 11 33 621.6 0 33 12 26 621.6 0 26 13 20 621.6 0 20 14 12 621.6 0 12 15 12 621.6 0 12 16 25 621.6 0 25 17 15 621.6 0 15 18 14 621.6 0 14 19 14 621.6 0 14 20 17 621.6 0 17 21 16 621.6 0 16 22 11 621.6 0 11 23 13 621.6 0 13 24 9 621.6 0 9 25 16 621.6 0 16 26 18 621.6 0 18 27 12 621.6 0 12 28 22 621.6 0 22 29 21 621.6 0 21 30 13 621.6 0 13 31 10 621.6 0 10 32 14 621.6 0 14 33 22 621.6 0 22 34 16 621.6 0 16 35 10 621.6 0 10 36 31 621.6 0 31 37 36 621.6 0 36 38 16 621.6 0 16 39 16 621.6 0 16 40 15 621.6 0 15 41 14 621.6 0 14 42 17 621.6 0 17 43 21 621.6 0 21 44 14 621.6 0 14 45 16 621.6 0 16 46 17 621.6 0 17 47 20 621.6 0 20 48 15 621.6 0 15 49 15 621.6 0 15 50 12 621.6 0 12 51 15 621.6 0 15 52 23 621.6 0 23 53 23 621.6 0 23 54 17 621.6 0 17 55 21 621.6 0 21 56 22 621.6 0 22 57 26 621.6 0 26 58 16 621.6 0 16 59 27 621.6 0 27 60 15 621.6 0 15 61 29 621.6 0 29 62 25 621.6 0 25 63 28 621.6 0 28 64 24 621.6 0 24 65 21 621.6 0 21 66 18 621.6 0 18 67 31 621.6 0 31 68 23 621.6 0 23 69 37 621.6 0 37 70 152 621.6 0 152 71 23 621.6 0 23 72 40 621.6 0 40 73 31 621.6 0 31 74 110 621.6 0 110 75 61 621.6 0 61 76 94 621.6 0 94 77 22 621.6 0 22 78 22 621.6 0 22 79 33 621.6 0 33 80 35 621.6 0 35 81 64 621.6 0 64 82 214 621.6 0 214 83 112 621.6 0 112 84 279 621.6 0 279 85 36 621.6 0 36 86 42 621.6 0 42 87 27 621.6 0 27 88 26 621.6 0 26 89 29 621.6 0 29 90 24 621.6 0 24 91 25 621.6 0 25 92 39 621.6 0 39 93 23 621.6 0 23 94 24 621.6 0 24 95 31 621.6 0 31 96 30 621.6 0 30 97 24 621.6 0 24 98 28 621.6 0 28 99 24 621.6 0 24 100 37 621.6 0 37 101 23 621.6 0 23 102 33 621.6 0 33 103 42 621.6 0 42 104 38 621.6 0 38 105 40 621.6 0 40 106 28 621.6 0 28 107 25 621.6 0 25 108 27 621.6 0 27 109 35 621.6 0 35 110 28 621.6 0 28 111 30 621.6 0 30 112 40 621.6 0 40 113 41 621.6 0 41 114 28 621.6 0 28 115 33 621.6 0 33 116 28 621.6 0 28 117 36 621.6 0 36 118 26 621.6 0 26 119 34 621.6 0 34 120 29 621.6 0 29 121 36 621.6 0 36 122 42 621.6 0 42 123 141 621.6 0 141 124 27 621.6 0 27 125 46 621.6 0 46 126 45 621.6 0 45 127 49 621.6 0 49 128 43 621.6 0 43 129 53 621.6 0 53 130 32 621.6 0 32 131 38 621.6 0 38 132 26 621.6 0 26 133 28 621.6 0 28 134 48 621.6 0 48 135 36 621.6 0 36 136 68 621.6 0 68 137 53 621.6 0 53 138 37 621.6 0 37 139 74 621.6 0 74 140 55 621.6 0 55 141 36 621.6 0 36 142 48 621.6 0 48 143 50 621.6 0 50 144 41 621.6 0 41 145 74 621.6 0 74 146 64 621.6 0 64 147 47 621.6 0 47 148 54 621.6 0 54 149 64 621.6 0 64 150 52 621.6 0 52 151 64 621.6 0 64 152 69 621.6 0 69 153 57 621.6 0 57 154 62 621.6 0 62 155 59 621.6 0 59 156 49 621.6 0 49 157 56 621.6 0 56 158 62 621.6 0 62 159 50 621.6 0 50 160 82 621.6 0 82 161 62 621.6 0 62 162 74 621.6 0 74 163 85 621.6 0 85 164 84 621.6 0 84 165 99 621.6 0 99 166 119 621.6 0 119 167 541 621.6 0 541 168 431 621.6 0 431 169 519 621.6 0 519 170 98 621.6 0 98 171 231 621.6 0 231 172 140 621.6 0 140 173 114 621.6 0 114 174 88 621.6 0 88 175 93 621.6 0 93 176 86 621.6 0 86 177 86 621.6 0 86 178 87 621.6 0 87 179 87 621.6 0 87 180 113 621.6 0 113 181 94 621.6 0 94 182 113 621.6 0 113 183 136 621.6 0 136 184 161 621.6 0 161 185 107 621.6 0 107 186 81 621.6 0 81 187 94 621.6 0 94 188 125 621.6 0 125 189 132 621.6 0 132 190 121 621.6 0 121 191 153 621.6 0 153 192 109 621.6 0 109 193 107 621.6 0 107 194 112 621.6 0 112 195 127 621.6 0 127 196 104 621.6 0 104 197 136 621.6 0 136 198 123 621.6 0 123 199 156 621.6 0 156 200 426 621.6 0 426 201 710 621.6 0 710 202 666 621.6 0 666 203 307 621.6 0 307 204 106 621.6 0 106 205 141 621.6 0 141 206 121 621.6 0 121 207 109 621.6 0 109 208 145 621.6 0 145 209 124 621.6 0 124 210 147 621.6 0 147 211 249 621.6 0 249 212 133 621.6 0 133 213 138 621.6 0 138 214 163 621.6 0 163 215 152 621.6 0 152 216 162 621.6 0 162 217 141 621.6 0 141 218 143 621.6 0 143 219 147 621.6 0 147 220 156 621.6 0 156 221 227 621.6 0 227 222 163 621.6 0 163 223 275 621.6 0 275 224 467 621.6 0 467 225 381 621.6 0 381 226 586 621.6 0 586 227 1386 621.6 0 1386 228 8080 621.6 0 8080 229 2751 621.6 0 2751 230 24857 621.6 0 24857 231 296 621.6 0 296 232 143 621.6 0 143 233 123 621.6 0 123 234 144 621.6 0 144 235 103 621.6 0 103 236 154 621.6 0 154 237 135 621.6 0 135 238 118 621.6 0 118 239 144 621.6 0 144 240 130 621.6 0 130 241 141 621.6 0 141 242 135 621.6 0 135 243 129 621.6 0 129 244 141 621.6 0 141 245 180 621.6 0 180 246 170 621.6 0 170 247 166 621.6 0 166 248 173 621.6 0 173 249 176 621.6 0 176 250 153 621.6 0 153 251 138 621.6 0 138 252 155 621.6 0 155 253 136 621.6 0 136 254 171 621.6 0 171 255 173 621.6 0 173 256 291 621.6 0 291 257 215 621.6 0 215 258 240 621.6 0 240 259 186 621.6 0 186 260 164 621.6 0 164 261 478 621.6 0 478 262 518 621.6 0 518 263 765 621.6 0 765 264 298 621.6 0 298 265 342 621.6 0 342 266 274 621.6 0 274 267 176 621.6 0 176 268 566 621.6 0 566 269 537 621.6 0 537 270 490 621.6 0 490 271 169 621.6 0 169 272 147 621.6 0 147 273 163 621.6 0 163 274 144 621.6 0 144 275 146 621.6 0 146 276 141 621.6 0 141 277 141 621.6 0 141 278 155 621.6 0 155 279 152 621.6 0 152 280 172 621.6 0 172 281 172 621.6 0 172 282 236 621.6 0 236 283 178 621.6 0 178 284 200 621.6 0 200 285 152 621.6 0 152 286 154 621.6 0 154 287 171 621.6 0 171 288 192 621.6 0 192 289 161 621.6 0 161 290 137 621.6 0 137 291 132 621.6 0 132 292 177 621.6 0 177 293 180 621.6 0 180 294 197 621.6 0 197 295 146 621.6 0 146 296 162 621.6 0 162 297 155 621.6 0 155 298 136 621.6 0 136 299 148 621.6 0 148 300 192 621.6 0 192 301 257 621.6 0 257 Finished in 15.12 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 319,407 Reads with adapters: 167,728 (52.5%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 167,692 (52.5%) Total basepairs processed: 91,892,326 bp Total written (filtered): 45,384,953 bp (49.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 167728 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 54 0.0 3 0 0 52 2 19 1237 0.0 3 0 1219 13 4 1 20 163645 0.0 4 161073 2492 68 11 1 21 401 0.0 4 83 312 6 22 24 0.0 4 6 0 16 1 1 23 94 0.0 4 2 0 1 79 12 24 7 0.0 4 2 0 0 0 5 25 3 0.0 4 3 26 1 0.0 4 0 0 0 1 27 2 0.0 4 1 0 0 1 32 1 0.0 4 1 37 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 60 1 0.0 4 1 86 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 32 0.0 4 0 0 0 0 32 127 2174 0.0 4 0 0 0 0 2174 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 134 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 1 8 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 236 2 0.0 4 0 0 0 0 2 237 2 0.0 4 0 0 0 0 2 282 4 0.0 4 0 0 0 0 4 283 4 0.0 4 0 0 0 0 4 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.95 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 167,692 Reads with adapters: 24,897 (14.8%) Reads written (passing filters): 167,692 (100.0%) Total basepairs processed: 45,384,953 bp Total written (filtered): 45,128,157 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 24897 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 2.4% G: 95.0% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 76 2620.2 0 76 4 26 655.0 0 26 5 22 163.8 0 22 6 60 40.9 0 60 7 5 10.2 0 5 8 7 2.6 0 7 9 12828 0.6 0 369 12459 10 8804 0.2 1 317 8487 11 2925 0.0 1 25 2900 12 17 0.0 1 0 17 13 11 0.0 1 0 11 14 5 0.0 1 0 5 22 4 0.0 1 3 1 30 1 0.0 1 1 41 2 0.0 1 2 47 1 0.0 1 1 52 1 0.0 1 1 58 1 0.0 1 0 1 76 1 0.0 1 1 82 1 0.0 1 0 1 97 1 0.0 1 1 109 1 0.0 1 0 1 112 1 0.0 1 0 1 121 1 0.0 1 0 1 147 1 0.0 1 1 148 2 0.0 1 2 150 7 0.0 1 0 7 157 1 0.0 1 1 158 1 0.0 1 1 160 1 0.0 1 1 169 13 0.0 1 13 170 3 0.0 1 3 172 1 0.0 1 0 1 176 1 0.0 1 1 177 1 0.0 1 1 208 48 0.0 1 47 1 209 1 0.0 1 1 210 2 0.0 1 2 228 2 0.0 1 2 257 8 0.0 1 6 2 270 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.