This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.74 s (27 us/read; 2.19 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 1,000,592 (39.3%) Reads that were too short: 29,003 (1.1%) Reads written (passing filters): 971,589 (38.2%) Total basepairs processed: 766,381,518 bp Total written (filtered): 262,533,486 bp (34.3%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1000592 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 509 621.6 0 509 7 12240 621.6 0 12240 8 811 621.6 0 811 9 11539 621.6 0 11539 10 572 621.6 0 572 11 1050 621.6 0 1050 12 12280 621.6 0 12280 13 3093 621.6 0 3093 14 403 621.6 0 403 15 993 621.6 0 993 16 517 621.6 0 517 17 735 621.6 0 735 18 2257 621.6 0 2257 19 1174 621.6 0 1174 20 1134 621.6 0 1134 21 346 621.6 0 346 22 530 621.6 0 530 23 1460 621.6 0 1460 24 1765 621.6 0 1765 25 3274 621.6 0 3274 26 5381 621.6 0 5381 27 5429 621.6 0 5429 28 13606 621.6 0 13606 29 32557 621.6 0 32557 30 323245 621.6 0 323245 31 204934 621.6 0 204934 32 315620 621.6 0 315620 33 1246 621.6 0 1246 34 120 621.6 0 120 35 233 621.6 0 233 36 197 621.6 0 197 37 466 621.6 0 466 38 57 621.6 0 57 39 43 621.6 0 43 40 43 621.6 0 43 41 57 621.6 0 57 42 36 621.6 0 36 43 28 621.6 0 28 44 37 621.6 0 37 45 58 621.6 0 58 46 42 621.6 0 42 47 45 621.6 0 45 48 34 621.6 0 34 49 35 621.6 0 35 50 37 621.6 0 37 51 50 621.6 0 50 52 35 621.6 0 35 53 42 621.6 0 42 54 375 621.6 0 375 55 454 621.6 0 454 56 566 621.6 0 566 57 48 621.6 0 48 58 24 621.6 0 24 59 20 621.6 0 20 60 21 621.6 0 21 61 28 621.6 0 28 62 24 621.6 0 24 63 18 621.6 0 18 64 22 621.6 0 22 65 33 621.6 0 33 66 27 621.6 0 27 67 27 621.6 0 27 68 50 621.6 0 50 69 47 621.6 0 47 70 85 621.6 0 85 71 46 621.6 0 46 72 35 621.6 0 35 73 33 621.6 0 33 74 32 621.6 0 32 75 28 621.6 0 28 76 24 621.6 0 24 77 18 621.6 0 18 78 15 621.6 0 15 79 15 621.6 0 15 80 44 621.6 0 44 81 30 621.6 0 30 82 45 621.6 0 45 83 37 621.6 0 37 84 44 621.6 0 44 85 26 621.6 0 26 86 52 621.6 0 52 87 74 621.6 0 74 88 39 621.6 0 39 89 74 621.6 0 74 90 34 621.6 0 34 91 42 621.6 0 42 92 31 621.6 0 31 93 17 621.6 0 17 94 25 621.6 0 25 95 28 621.6 0 28 96 20 621.6 0 20 97 42 621.6 0 42 98 35 621.6 0 35 99 43 621.6 0 43 100 22 621.6 0 22 101 57 621.6 0 57 102 239 621.6 0 239 103 314 621.6 0 314 104 746 621.6 0 746 105 415 621.6 0 415 106 300 621.6 0 300 107 33 621.6 0 33 108 22 621.6 0 22 109 29 621.6 0 29 110 29 621.6 0 29 111 40 621.6 0 40 112 67 621.6 0 67 113 65 621.6 0 65 114 79 621.6 0 79 115 38 621.6 0 38 116 37 621.6 0 37 117 49 621.6 0 49 118 36 621.6 0 36 119 44 621.6 0 44 120 38 621.6 0 38 121 33 621.6 0 33 122 38 621.6 0 38 123 43 621.6 0 43 124 198 621.6 0 198 125 97 621.6 0 97 126 163 621.6 0 163 127 51 621.6 0 51 128 42 621.6 0 42 129 38 621.6 0 38 130 32 621.6 0 32 131 28 621.6 0 28 132 37 621.6 0 37 133 47 621.6 0 47 134 45 621.6 0 45 135 56 621.6 0 56 136 79 621.6 0 79 137 565 621.6 0 565 138 185 621.6 0 185 139 401 621.6 0 401 140 109 621.6 0 109 141 172 621.6 0 172 142 132 621.6 0 132 143 257 621.6 0 257 144 43 621.6 0 43 145 37 621.6 0 37 146 53 621.6 0 53 147 42 621.6 0 42 148 50 621.6 0 50 149 93 621.6 0 93 150 51 621.6 0 51 151 70 621.6 0 70 152 43 621.6 0 43 153 24 621.6 0 24 154 39 621.6 0 39 155 26 621.6 0 26 156 33 621.6 0 33 157 38 621.6 0 38 158 51 621.6 0 51 159 95 621.6 0 95 160 81 621.6 0 81 161 164 621.6 0 164 162 40 621.6 0 40 163 51 621.6 0 51 164 38 621.6 0 38 165 34 621.6 0 34 166 50 621.6 0 50 167 51 621.6 0 51 168 64 621.6 0 64 169 36 621.6 0 36 170 32 621.6 0 32 171 38 621.6 0 38 172 36 621.6 0 36 173 30 621.6 0 30 174 22 621.6 0 22 175 27 621.6 0 27 176 41 621.6 0 41 177 43 621.6 0 43 178 22 621.6 0 22 179 44 621.6 0 44 180 29 621.6 0 29 181 68 621.6 0 68 182 85 621.6 0 85 183 88 621.6 0 88 184 71 621.6 0 71 185 53 621.6 0 53 186 55 621.6 0 55 187 46 621.6 0 46 188 40 621.6 0 40 189 54 621.6 0 54 190 31 621.6 0 31 191 25 621.6 0 25 192 31 621.6 0 31 193 24 621.6 0 24 194 33 621.6 0 33 195 35 621.6 0 35 196 46 621.6 0 46 197 27 621.6 0 27 198 32 621.6 0 32 199 44 621.6 0 44 200 44 621.6 0 44 201 57 621.6 0 57 202 38 621.6 0 38 203 29 621.6 0 29 204 97 621.6 0 97 205 262 621.6 0 262 206 276 621.6 0 276 207 227 621.6 0 227 208 45 621.6 0 45 209 29 621.6 0 29 210 35 621.6 0 35 211 67 621.6 0 67 212 57 621.6 0 57 213 72 621.6 0 72 214 45 621.6 0 45 215 52 621.6 0 52 216 46 621.6 0 46 217 47 621.6 0 47 218 48 621.6 0 48 219 60 621.6 0 60 220 59 621.6 0 59 221 44 621.6 0 44 222 30 621.6 0 30 223 40 621.6 0 40 224 55 621.6 0 55 225 55 621.6 0 55 226 186 621.6 0 186 227 91 621.6 0 91 228 152 621.6 0 152 229 61 621.6 0 61 230 46 621.6 0 46 231 100 621.6 0 100 232 134 621.6 0 134 233 108 621.6 0 108 234 57 621.6 0 57 235 32 621.6 0 32 236 55 621.6 0 55 237 62 621.6 0 62 238 58 621.6 0 58 239 55 621.6 0 55 240 70 621.6 0 70 241 72 621.6 0 72 242 57 621.6 0 57 243 68 621.6 0 68 244 60 621.6 0 60 245 68 621.6 0 68 246 98 621.6 0 98 247 140 621.6 0 140 248 86 621.6 0 86 249 93 621.6 0 93 250 130 621.6 0 130 251 98 621.6 0 98 252 124 621.6 0 124 253 184 621.6 0 184 254 1419 621.6 0 1419 255 1509 621.6 0 1509 256 2643 621.6 0 2643 257 425 621.6 0 425 258 387 621.6 0 387 259 302 621.6 0 302 260 2935 621.6 0 2935 261 2922 621.6 0 2922 262 5375 621.6 0 5375 263 98 621.6 0 98 264 51 621.6 0 51 265 78 621.6 0 78 266 62 621.6 0 62 267 114 621.6 0 114 268 135 621.6 0 135 269 176 621.6 0 176 270 108 621.6 0 108 271 67 621.6 0 67 272 84 621.6 0 84 273 95 621.6 0 95 274 125 621.6 0 125 275 284 621.6 0 284 276 335 621.6 0 335 277 554 621.6 0 554 278 484 621.6 0 484 279 177 621.6 0 177 280 873 621.6 0 873 281 832 621.6 0 832 282 828 621.6 0 828 283 188 621.6 0 188 284 42 621.6 0 42 285 43 621.6 0 43 286 60 621.6 0 60 287 50 621.6 0 50 288 65 621.6 0 65 289 66 621.6 0 66 290 39 621.6 0 39 291 66 621.6 0 66 292 44 621.6 0 44 293 73 621.6 0 73 294 72 621.6 0 72 295 68 621.6 0 68 296 80 621.6 0 80 297 33 621.6 0 33 298 29 621.6 0 29 299 49 621.6 0 49 300 45 621.6 0 45 301 54 621.6 0 54 Finished in 39.78 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 971,589 Reads with adapters: 13,654 (1.4%) Reads that were too short: 755 (0.1%) Reads written (passing filters): 12,899 (1.3%) Total basepairs processed: 262,533,486 bp Total written (filtered): 3,468,583 bp (1.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13654 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 13 0.0 3 0 0 10 3 16 211 0.0 3 0 180 23 8 17 12023 0.0 3 11076 803 111 33 18 42 0.0 3 2 25 3 12 19 58 0.0 3 11 3 21 23 20 14 0.0 3 1 1 1 11 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 1 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 1 39 1 0.0 3 0 1 40 1 0.0 3 0 0 1 41 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 51 4 0.0 3 0 0 0 4 52 7 0.0 3 0 0 0 7 53 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 1 2 55 5 0.0 3 0 0 0 5 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 9 0.0 3 0 0 0 9 68 11 0.0 3 0 1 1 9 69 12 0.0 3 0 0 2 10 70 11 0.0 3 0 0 1 10 71 1 0.0 3 0 0 0 1 72 4 0.0 3 0 0 0 4 73 3 0.0 3 0 0 0 3 75 2 0.0 3 0 0 0 2 76 7 0.0 3 0 0 1 6 87 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 4 0.0 3 0 0 0 4 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 7 0.0 3 0 0 0 7 97 1 0.0 3 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 104 9 0.0 3 1 0 0 8 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 112 5 0.0 3 1 0 1 3 113 5 0.0 3 0 0 0 5 114 3 0.0 3 1 0 0 2 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 7 0.0 3 0 0 0 7 119 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 4 0.0 3 0 0 0 4 126 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 133 17 0.0 3 0 0 0 17 134 2 0.0 3 0 0 0 2 136 2 0.0 3 0 0 0 2 137 13 0.0 3 0 0 1 12 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 1 1 141 16 0.0 3 0 0 0 16 142 5 0.0 3 0 0 0 5 143 1 0.0 3 0 0 1 145 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 25 0.0 3 0 0 0 25 157 5 0.0 3 0 0 0 5 158 1 0.0 3 0 0 0 1 159 5 0.0 3 0 0 0 5 160 9 0.0 3 0 0 0 9 161 50 0.0 3 0 0 0 50 162 12 0.0 3 0 0 0 12 163 3 0.0 3 0 0 0 3 164 9 0.0 3 0 0 0 9 165 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 1 1 167 11 0.0 3 0 0 0 11 168 11 0.0 3 0 0 0 11 169 21 0.0 3 0 0 0 21 170 2 0.0 3 0 0 0 2 171 61 0.0 3 0 0 1 60 172 11 0.0 3 0 0 0 11 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 1 176 2 0.0 3 0 0 0 2 178 4 0.0 3 0 0 0 4 179 3 0.0 3 0 0 0 3 180 6 0.0 3 0 0 0 6 181 67 0.0 3 0 0 0 67 182 62 0.0 3 0 0 2 60 183 10 0.0 3 0 0 1 9 184 9 0.0 3 0 0 0 9 185 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 190 4 0.0 3 0 0 0 4 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 4 0.0 3 0 0 0 4 194 5 0.0 3 0 0 0 5 195 13 0.0 3 0 0 0 13 196 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 198 27 0.0 3 0 0 0 27 199 6 0.0 3 0 0 0 6 200 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 202 7 0.0 3 0 0 0 7 203 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 212 2 0.0 3 0 0 0 2 213 3 0.0 3 0 0 0 3 214 6 0.0 3 0 0 0 6 215 14 0.0 3 0 0 0 14 216 5 0.0 3 0 0 0 5 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 6 0.0 3 0 0 0 6 230 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 234 2 0.0 3 0 0 1 1 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 241 3 0.0 3 0 0 0 3 244 2 0.0 3 0 0 0 2 245 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 16 0.0 3 0 0 0 16 248 6 0.0 3 0 0 0 6 249 2 0.0 3 0 0 0 2 251 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 255 9 0.0 3 0 0 0 9 256 59 0.0 3 0 0 0 59 257 66 0.0 3 0 0 1 65 258 145 0.0 3 0 0 5 140 259 86 0.0 3 0 0 3 83 260 17 0.0 3 0 0 1 16 261 1 0.0 3 0 0 0 1 262 4 0.0 3 0 0 0 4 263 2 0.0 3 0 0 0 2 264 3 0.0 3 0 0 0 3 265 11 0.0 3 0 0 0 11 266 6 0.0 3 0 0 0 6 267 5 0.0 3 0 0 0 5 268 4 0.0 3 0 0 0 4 269 8 0.0 3 0 0 0 8 270 4 0.0 3 0 0 0 4 271 2 0.0 3 0 0 0 2 272 1 0.0 3 0 0 0 1 273 3 0.0 3 0 0 0 3 274 2 0.0 3 0 0 0 2 276 2 0.0 3 0 0 0 2 277 5 0.0 3 0 0 0 5 278 2 0.0 3 0 0 0 2 279 4 0.0 3 0 0 0 4 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 7 0.0 3 0 0 0 7 283 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 12,899 Reads with adapters: 32 (0.2%) Reads written (passing filters): 12,899 (100.0%) Total basepairs processed: 3,468,583 bp Total written (filtered): 3,468,477 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 32 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.4% C: 9.4% G: 46.9% T: 9.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 24 201.5 0 24 4 7 50.4 0 7 6 1 3.1 0 1