This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.93 s (28 us/read; 2.15 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 1,195,267 (46.9%) Reads that were too short: 38,371 (1.5%) Reads written (passing filters): 1,156,896 (45.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 312,829,130 bp (40.8%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1195267 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 343 621.6 0 343 7 14174 621.6 0 14174 8 677 621.6 0 677 9 11107 621.6 0 11107 10 518 621.6 0 518 11 752 621.6 0 752 12 11608 621.6 0 11608 13 3532 621.6 0 3532 14 504 621.6 0 504 15 1045 621.6 0 1045 16 578 621.6 0 578 17 816 621.6 0 816 18 2943 621.6 0 2943 19 1444 621.6 0 1444 20 1423 621.6 0 1423 21 414 621.6 0 414 22 591 621.6 0 591 23 1391 621.6 0 1391 24 1771 621.6 0 1771 25 3307 621.6 0 3307 26 5691 621.6 0 5691 27 5801 621.6 0 5801 28 15064 621.6 0 15064 29 34266 621.6 0 34266 30 399086 621.6 0 399086 31 245647 621.6 0 245647 32 381521 621.6 0 381521 33 1395 621.6 0 1395 34 162 621.6 0 162 35 181 621.6 0 181 36 135 621.6 0 135 37 211 621.6 0 211 38 27 621.6 0 27 39 27 621.6 0 27 40 27 621.6 0 27 41 29 621.6 0 29 42 25 621.6 0 25 43 35 621.6 0 35 44 44 621.6 0 44 45 56 621.6 0 56 46 34 621.6 0 34 47 39 621.6 0 39 48 25 621.6 0 25 49 24 621.6 0 24 50 31 621.6 0 31 51 29 621.6 0 29 52 26 621.6 0 26 53 32 621.6 0 32 54 153 621.6 0 153 55 106 621.6 0 106 56 157 621.6 0 157 57 20 621.6 0 20 58 28 621.6 0 28 59 23 621.6 0 23 60 23 621.6 0 23 61 37 621.6 0 37 62 33 621.6 0 33 63 18 621.6 0 18 64 23 621.6 0 23 65 43 621.6 0 43 66 24 621.6 0 24 67 28 621.6 0 28 68 33 621.6 0 33 69 27 621.6 0 27 70 30 621.6 0 30 71 42 621.6 0 42 72 26 621.6 0 26 73 25 621.6 0 25 74 31 621.6 0 31 75 27 621.6 0 27 76 29 621.6 0 29 77 28 621.6 0 28 78 25 621.6 0 25 79 35 621.6 0 35 80 37 621.6 0 37 81 28 621.6 0 28 82 57 621.6 0 57 83 67 621.6 0 67 84 46 621.6 0 46 85 55 621.6 0 55 86 63 621.6 0 63 87 102 621.6 0 102 88 56 621.6 0 56 89 92 621.6 0 92 90 41 621.6 0 41 91 45 621.6 0 45 92 43 621.6 0 43 93 33 621.6 0 33 94 47 621.6 0 47 95 42 621.6 0 42 96 29 621.6 0 29 97 36 621.6 0 36 98 34 621.6 0 34 99 34 621.6 0 34 100 42 621.6 0 42 101 41 621.6 0 41 102 115 621.6 0 115 103 121 621.6 0 121 104 300 621.6 0 300 105 253 621.6 0 253 106 148 621.6 0 148 107 37 621.6 0 37 108 36 621.6 0 36 109 32 621.6 0 32 110 31 621.6 0 31 111 49 621.6 0 49 112 37 621.6 0 37 113 74 621.6 0 74 114 85 621.6 0 85 115 46 621.6 0 46 116 41 621.6 0 41 117 77 621.6 0 77 118 48 621.6 0 48 119 48 621.6 0 48 120 50 621.6 0 50 121 46 621.6 0 46 122 42 621.6 0 42 123 44 621.6 0 44 124 229 621.6 0 229 125 97 621.6 0 97 126 176 621.6 0 176 127 53 621.6 0 53 128 46 621.6 0 46 129 47 621.6 0 47 130 33 621.6 0 33 131 35 621.6 0 35 132 34 621.6 0 34 133 50 621.6 0 50 134 36 621.6 0 36 135 37 621.6 0 37 136 46 621.6 0 46 137 133 621.6 0 133 138 75 621.6 0 75 139 144 621.6 0 144 140 39 621.6 0 39 141 71 621.6 0 71 142 67 621.6 0 67 143 120 621.6 0 120 144 44 621.6 0 44 145 22 621.6 0 22 146 56 621.6 0 56 147 39 621.6 0 39 148 39 621.6 0 39 149 84 621.6 0 84 150 50 621.6 0 50 151 72 621.6 0 72 152 33 621.6 0 33 153 27 621.6 0 27 154 42 621.6 0 42 155 39 621.6 0 39 156 45 621.6 0 45 157 53 621.6 0 53 158 37 621.6 0 37 159 92 621.6 0 92 160 98 621.6 0 98 161 189 621.6 0 189 162 34 621.6 0 34 163 48 621.6 0 48 164 33 621.6 0 33 165 32 621.6 0 32 166 42 621.6 0 42 167 39 621.6 0 39 168 65 621.6 0 65 169 55 621.6 0 55 170 45 621.6 0 45 171 38 621.6 0 38 172 37 621.6 0 37 173 38 621.6 0 38 174 31 621.6 0 31 175 45 621.6 0 45 176 38 621.6 0 38 177 61 621.6 0 61 178 38 621.6 0 38 179 34 621.6 0 34 180 32 621.6 0 32 181 51 621.6 0 51 182 57 621.6 0 57 183 64 621.6 0 64 184 41 621.6 0 41 185 41 621.6 0 41 186 42 621.6 0 42 187 29 621.6 0 29 188 46 621.6 0 46 189 53 621.6 0 53 190 52 621.6 0 52 191 44 621.6 0 44 192 50 621.6 0 50 193 45 621.6 0 45 194 43 621.6 0 43 195 40 621.6 0 40 196 35 621.6 0 35 197 36 621.6 0 36 198 49 621.6 0 49 199 52 621.6 0 52 200 46 621.6 0 46 201 54 621.6 0 54 202 57 621.6 0 57 203 35 621.6 0 35 204 49 621.6 0 49 205 106 621.6 0 106 206 120 621.6 0 120 207 115 621.6 0 115 208 42 621.6 0 42 209 32 621.6 0 32 210 48 621.6 0 48 211 45 621.6 0 45 212 53 621.6 0 53 213 53 621.6 0 53 214 52 621.6 0 52 215 43 621.6 0 43 216 52 621.6 0 52 217 52 621.6 0 52 218 43 621.6 0 43 219 51 621.6 0 51 220 74 621.6 0 74 221 48 621.6 0 48 222 47 621.6 0 47 223 53 621.6 0 53 224 33 621.6 0 33 225 49 621.6 0 49 226 96 621.6 0 96 227 61 621.6 0 61 228 109 621.6 0 109 229 55 621.6 0 55 230 52 621.6 0 52 231 47 621.6 0 47 232 72 621.6 0 72 233 74 621.6 0 74 234 32 621.6 0 32 235 43 621.6 0 43 236 60 621.6 0 60 237 45 621.6 0 45 238 62 621.6 0 62 239 68 621.6 0 68 240 58 621.6 0 58 241 94 621.6 0 94 242 73 621.6 0 73 243 82 621.6 0 82 244 68 621.6 0 68 245 90 621.6 0 90 246 146 621.6 0 146 247 204 621.6 0 204 248 99 621.6 0 99 249 111 621.6 0 111 250 174 621.6 0 174 251 84 621.6 0 84 252 120 621.6 0 120 253 190 621.6 0 190 254 1455 621.6 0 1455 255 1664 621.6 0 1664 256 2897 621.6 0 2897 257 424 621.6 0 424 258 394 621.6 0 394 259 335 621.6 0 335 260 5440 621.6 0 5440 261 5248 621.6 0 5248 262 9592 621.6 0 9592 263 113 621.6 0 113 264 54 621.6 0 54 265 85 621.6 0 85 266 59 621.6 0 59 267 81 621.6 0 81 268 76 621.6 0 76 269 84 621.6 0 84 270 85 621.6 0 85 271 73 621.6 0 73 272 167 621.6 0 167 273 102 621.6 0 102 274 156 621.6 0 156 275 312 621.6 0 312 276 644 621.6 0 644 277 896 621.6 0 896 278 1037 621.6 0 1037 279 262 621.6 0 262 280 614 621.6 0 614 281 546 621.6 0 546 282 643 621.6 0 643 283 169 621.6 0 169 284 51 621.6 0 51 285 36 621.6 0 36 286 46 621.6 0 46 287 50 621.6 0 50 288 51 621.6 0 51 289 66 621.6 0 66 290 35 621.6 0 35 291 53 621.6 0 53 292 47 621.6 0 47 293 61 621.6 0 61 294 69 621.6 0 69 295 50 621.6 0 50 296 47 621.6 0 47 297 21 621.6 0 21 298 31 621.6 0 31 299 37 621.6 0 37 300 37 621.6 0 37 301 55 621.6 0 55 Finished in 52.87 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 1,156,896 Reads with adapters: 14,464 (1.3%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 14,436 (1.2%) Total basepairs processed: 312,829,130 bp Total written (filtered): 3,920,506 bp (1.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14464 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 7 19 137 0.0 3 0 137 20 13942 0.0 4 13733 203 5 1 21 23 0.0 4 7 15 1 22 2 0.0 4 1 0 1 23 6 0.0 4 0 0 0 6 32 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 61 1 0.0 4 1 63 2 0.0 4 2 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 73 1 0.0 4 1 74 1 0.0 4 0 1 80 1 0.0 4 1 84 50 0.0 4 0 0 0 0 50 94 1 0.0 4 1 102 1 0.0 4 1 106 1 0.0 4 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 1 118 1 0.0 4 1 119 1 0.0 4 0 1 121 1 0.0 4 0 1 124 153 0.0 4 0 0 0 0 153 126 1 0.0 4 0 0 0 0 1 127 51 0.0 4 1 0 0 0 50 129 1 0.0 4 1 132 2 0.0 4 2 147 1 0.0 4 0 0 0 0 1 148 19 0.0 4 0 0 0 0 19 149 13 0.0 4 0 0 0 0 13 150 12 0.0 4 0 0 0 0 12 154 2 0.0 4 0 0 0 0 2 167 1 0.0 4 0 0 0 0 1 172 1 0.0 4 1 173 1 0.0 4 1 179 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 193 2 0.0 4 0 0 0 0 2 195 1 0.0 4 0 0 0 0 1 200 1 0.0 4 1 201 1 0.0 4 1 202 1 0.0 4 0 1 207 1 0.0 4 0 1 214 1 0.0 4 0 0 0 0 1 225 4 0.0 4 0 1 0 0 3 227 1 0.0 4 0 1 228 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.51 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 14,436 Reads with adapters: 763 (5.3%) Reads written (passing filters): 14,436 (100.0%) Total basepairs processed: 3,920,506 bp Total written (filtered): 3,881,175 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 763 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.5% C: 3.3% G: 71.2% T: 20.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 50 225.6 0 50 4 6 56.4 0 6 9 2 0.1 0 2 10 8 0.0 1 1 7 11 516 0.0 1 2 514 12 32 0.0 1 1 31 15 1 0.0 1 0 1 113 2 0.0 1 1 1 125 1 0.0 1 1 225 137 0.0 1 118 19 226 8 0.0 1 8