This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.61 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 262,563 (10.3%) Reads that were too short: 28,768 (1.1%) Reads written (passing filters): 233,795 (9.2%) Total basepairs processed: 766,381,518 bp Total written (filtered): 63,144,302 bp (8.2%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 262563 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1251 621.6 0 1251 7 2086 621.6 0 2086 8 89643 621.6 0 89643 9 82728 621.6 0 82728 10 143 621.6 0 143 11 49 621.6 0 49 12 58 621.6 0 58 13 54 621.6 0 54 14 41 621.6 0 41 15 60 621.6 0 60 16 49 621.6 0 49 17 55 621.6 0 55 18 78 621.6 0 78 19 44 621.6 0 44 20 49 621.6 0 49 21 41 621.6 0 41 22 47 621.6 0 47 23 67 621.6 0 67 24 58 621.6 0 58 25 73 621.6 0 73 26 64 621.6 0 64 27 46 621.6 0 46 28 46 621.6 0 46 29 51 621.6 0 51 30 50 621.6 0 50 31 52 621.6 0 52 32 54 621.6 0 54 33 60 621.6 0 60 34 35 621.6 0 35 35 68 621.6 0 68 36 51 621.6 0 51 37 52 621.6 0 52 38 40 621.6 0 40 39 78 621.6 0 78 40 36 621.6 0 36 41 64 621.6 0 64 42 102 621.6 0 102 43 58 621.6 0 58 44 51 621.6 0 51 45 62 621.6 0 62 46 46 621.6 0 46 47 58 621.6 0 58 48 41 621.6 0 41 49 56 621.6 0 56 50 54 621.6 0 54 51 64 621.6 0 64 52 110 621.6 0 110 53 85 621.6 0 85 54 697 621.6 0 697 55 107 621.6 0 107 56 55 621.6 0 55 57 95 621.6 0 95 58 63 621.6 0 63 59 69 621.6 0 69 60 54 621.6 0 54 61 80 621.6 0 80 62 86 621.6 0 86 63 126 621.6 0 126 64 129 621.6 0 129 65 193 621.6 0 193 66 248 621.6 0 248 67 611 621.6 0 611 68 1461 621.6 0 1461 69 18640 621.6 0 18640 70 3646 621.6 0 3646 71 5369 621.6 0 5369 72 65 621.6 0 65 73 45 621.6 0 45 74 54 621.6 0 54 75 62 621.6 0 62 76 51 621.6 0 51 77 117 621.6 0 117 78 63 621.6 0 63 79 74 621.6 0 74 80 234 621.6 0 234 81 112 621.6 0 112 82 154 621.6 0 154 83 67 621.6 0 67 84 43 621.6 0 43 85 47 621.6 0 47 86 75 621.6 0 75 87 109 621.6 0 109 88 93 621.6 0 93 89 85 621.6 0 85 90 108 621.6 0 108 91 84 621.6 0 84 92 73 621.6 0 73 93 80 621.6 0 80 94 67 621.6 0 67 95 63 621.6 0 63 96 184 621.6 0 184 97 126 621.6 0 126 98 96 621.6 0 96 99 99 621.6 0 99 100 556 621.6 0 556 101 837 621.6 0 837 102 1632 621.6 0 1632 103 99 621.6 0 99 104 61 621.6 0 61 105 70 621.6 0 70 106 61 621.6 0 61 107 59 621.6 0 59 108 60 621.6 0 60 109 49 621.6 0 49 110 78 621.6 0 78 111 73 621.6 0 73 112 75 621.6 0 75 113 103 621.6 0 103 114 84 621.6 0 84 115 86 621.6 0 86 116 86 621.6 0 86 117 81 621.6 0 81 118 88 621.6 0 88 119 97 621.6 0 97 120 105 621.6 0 105 121 72 621.6 0 72 122 104 621.6 0 104 123 111 621.6 0 111 124 101 621.6 0 101 125 89 621.6 0 89 126 79 621.6 0 79 127 92 621.6 0 92 128 64 621.6 0 64 129 71 621.6 0 71 130 111 621.6 0 111 131 123 621.6 0 123 132 395 621.6 0 395 133 242 621.6 0 242 134 494 621.6 0 494 135 170 621.6 0 170 136 263 621.6 0 263 137 353 621.6 0 353 138 406 621.6 0 406 139 115 621.6 0 115 140 99 621.6 0 99 141 66 621.6 0 66 142 73 621.6 0 73 143 93 621.6 0 93 144 104 621.6 0 104 145 76 621.6 0 76 146 101 621.6 0 101 147 102 621.6 0 102 148 107 621.6 0 107 149 82 621.6 0 82 150 95 621.6 0 95 151 112 621.6 0 112 152 91 621.6 0 91 153 102 621.6 0 102 154 133 621.6 0 133 155 73 621.6 0 73 156 139 621.6 0 139 157 207 621.6 0 207 158 215 621.6 0 215 159 469 621.6 0 469 160 147 621.6 0 147 161 130 621.6 0 130 162 112 621.6 0 112 163 125 621.6 0 125 164 113 621.6 0 113 165 108 621.6 0 108 166 87 621.6 0 87 167 236 621.6 0 236 168 192 621.6 0 192 169 158 621.6 0 158 170 158 621.6 0 158 171 127 621.6 0 127 172 150 621.6 0 150 173 106 621.6 0 106 174 117 621.6 0 117 175 113 621.6 0 113 176 302 621.6 0 302 177 693 621.6 0 693 178 708 621.6 0 708 179 922 621.6 0 922 180 347 621.6 0 347 181 353 621.6 0 353 182 918 621.6 0 918 183 421 621.6 0 421 184 1090 621.6 0 1090 185 395 621.6 0 395 186 139 621.6 0 139 187 183 621.6 0 183 188 149 621.6 0 149 189 215 621.6 0 215 190 176 621.6 0 176 191 134 621.6 0 134 192 129 621.6 0 129 193 107 621.6 0 107 194 116 621.6 0 116 195 106 621.6 0 106 196 101 621.6 0 101 197 200 621.6 0 200 198 183 621.6 0 183 199 185 621.6 0 185 200 188 621.6 0 188 201 155 621.6 0 155 202 1110 621.6 0 1110 203 953 621.6 0 953 204 1194 621.6 0 1194 205 516 621.6 0 516 206 110 621.6 0 110 207 80 621.6 0 80 208 94 621.6 0 94 209 127 621.6 0 127 210 131 621.6 0 131 211 209 621.6 0 209 212 182 621.6 0 182 213 157 621.6 0 157 214 127 621.6 0 127 215 120 621.6 0 120 216 123 621.6 0 123 217 151 621.6 0 151 218 157 621.6 0 157 219 152 621.6 0 152 220 138 621.6 0 138 221 144 621.6 0 144 222 393 621.6 0 393 223 496 621.6 0 496 224 600 621.6 0 600 225 363 621.6 0 363 226 184 621.6 0 184 227 502 621.6 0 502 228 230 621.6 0 230 229 340 621.6 0 340 230 100 621.6 0 100 231 99 621.6 0 99 232 102 621.6 0 102 233 94 621.6 0 94 234 94 621.6 0 94 235 104 621.6 0 104 236 76 621.6 0 76 237 107 621.6 0 107 238 137 621.6 0 137 239 102 621.6 0 102 240 136 621.6 0 136 241 128 621.6 0 128 242 476 621.6 0 476 243 502 621.6 0 502 244 492 621.6 0 492 245 228 621.6 0 228 246 116 621.6 0 116 247 107 621.6 0 107 248 78 621.6 0 78 249 81 621.6 0 81 250 112 621.6 0 112 251 192 621.6 0 192 252 181 621.6 0 181 253 271 621.6 0 271 254 130 621.6 0 130 255 318 621.6 0 318 256 294 621.6 0 294 257 325 621.6 0 325 258 234 621.6 0 234 259 157 621.6 0 157 260 137 621.6 0 137 261 140 621.6 0 140 262 165 621.6 0 165 263 176 621.6 0 176 264 244 621.6 0 244 265 449 621.6 0 449 266 331 621.6 0 331 267 352 621.6 0 352 268 271 621.6 0 271 269 2241 621.6 0 2241 270 677 621.6 0 677 271 1997 621.6 0 1997 272 285 621.6 0 285 273 419 621.6 0 419 274 201 621.6 0 201 275 158 621.6 0 158 276 330 621.6 0 330 277 411 621.6 0 411 278 398 621.6 0 398 279 302 621.6 0 302 280 123 621.6 0 123 281 111 621.6 0 111 282 97 621.6 0 97 283 162 621.6 0 162 284 182 621.6 0 182 285 210 621.6 0 210 286 195 621.6 0 195 287 133 621.6 0 133 288 113 621.6 0 113 289 130 621.6 0 130 290 153 621.6 0 153 291 614 621.6 0 614 292 465 621.6 0 465 293 429 621.6 0 429 294 286 621.6 0 286 295 93 621.6 0 93 296 89 621.6 0 89 297 110 621.6 0 110 298 132 621.6 0 132 299 217 621.6 0 217 300 212 621.6 0 212 301 172 621.6 0 172 Finished in 10.50 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 233,795 Reads with adapters: 89,245 (38.2%) Reads that were too short: 218 (0.1%) Reads written (passing filters): 89,027 (38.1%) Total basepairs processed: 63,144,302 bp Total written (filtered): 24,539,204 bp (38.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 89245 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 27 0.0 2 0 0 0 27 15 121 0.0 3 0 0 78 43 16 1003 0.0 3 0 809 102 92 17 86838 0.0 3 79651 5777 1100 310 18 262 0.0 3 17 198 24 23 19 400 0.0 3 1 3 227 169 20 157 0.0 3 84 7 4 62 21 3 0.0 3 1 1 0 1 22 7 0.0 3 0 1 0 6 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 1 28 2 0.0 3 2 29 1 0.0 3 1 31 4 0.0 3 0 0 0 4 34 18 0.0 3 0 0 0 18 35 4 0.0 3 0 0 0 4 37 5 0.0 3 0 0 0 5 38 1 0.0 3 0 0 0 1 40 6 0.0 3 0 0 1 5 42 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 5 0.0 3 0 0 0 5 49 4 0.0 3 0 0 0 4 53 1 0.0 3 0 0 0 1 55 21 0.0 3 0 0 1 20 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 5 0.0 3 0 0 0 5 60 5 0.0 3 0 0 0 5 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 6 0.0 3 0 0 0 6 74 5 0.0 3 0 0 0 5 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 2 0.0 3 0 0 0 2 90 4 0.0 3 0 0 0 4 91 81 0.0 3 0 0 2 79 92 4 0.0 3 0 0 0 4 94 11 0.0 3 0 0 2 9 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 1 98 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 2 0.0 3 0 0 1 1 101 6 0.0 3 0 0 0 6 102 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 0 4 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 1 1 117 2 0.0 3 0 0 0 2 118 3 0.0 3 0 0 0 3 119 7 0.0 3 0 0 0 7 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 6 0.0 3 0 0 0 6 144 2 0.0 3 0 0 0 2 146 6 0.0 3 0 0 0 6 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 156 6 0.0 3 0 0 0 6 158 1 0.0 3 0 0 0 1 159 5 0.0 3 0 0 0 5 160 2 0.0 3 0 0 0 2 161 2 0.0 3 0 0 1 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 1 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 3 0.0 3 0 0 0 3 192 11 0.0 3 0 0 0 11 193 6 0.0 3 0 0 1 5 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 199 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 218 2 0.0 3 0 0 0 2 219 9 0.0 3 0 0 1 8 220 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 4 0.0 3 0 0 0 4 279 1 0.0 3 0 0 0 1 280 8 0.0 3 0 0 0 8 281 9 0.0 3 0 0 1 8 282 2 0.0 3 0 0 1 1 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 286 2 0.0 3 0 0 0 2 288 2 0.0 3 0 0 0 2 289 3 0.0 3 0 0 0 3 291 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.10 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 89,027 Reads with adapters: 112 (0.1%) Reads written (passing filters): 89,027 (100.0%) Total basepairs processed: 24,539,204 bp Total written (filtered): 24,538,783 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 112 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.6% C: 17.9% G: 38.4% T: 7.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 66 1391.0 0 66 4 32 347.8 0 32 5 9 86.9 0 9 10 5 0.1 1 0 5