This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.71 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 254,075 (10.0%) Reads that were too short: 15,790 (0.6%) Reads written (passing filters): 238,285 (9.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 65,414,197 bp (8.5%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 254075 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1073 621.6 0 1073 7 1649 621.6 0 1649 8 88172 621.6 0 88172 9 93176 621.6 0 93176 10 162 621.6 0 162 11 64 621.6 0 64 12 68 621.6 0 68 13 61 621.6 0 61 14 62 621.6 0 62 15 60 621.6 0 60 16 49 621.6 0 49 17 48 621.6 0 48 18 91 621.6 0 91 19 89 621.6 0 89 20 52 621.6 0 52 21 42 621.6 0 42 22 65 621.6 0 65 23 50 621.6 0 50 24 54 621.6 0 54 25 63 621.6 0 63 26 46 621.6 0 46 27 54 621.6 0 54 28 49 621.6 0 49 29 54 621.6 0 54 30 60 621.6 0 60 31 67 621.6 0 67 32 78 621.6 0 78 33 459 621.6 0 459 34 89 621.6 0 89 35 82 621.6 0 82 36 84 621.6 0 84 37 47 621.6 0 47 38 51 621.6 0 51 39 67 621.6 0 67 40 53 621.6 0 53 41 57 621.6 0 57 42 120 621.6 0 120 43 102 621.6 0 102 44 69 621.6 0 69 45 70 621.6 0 70 46 78 621.6 0 78 47 55 621.6 0 55 48 62 621.6 0 62 49 55 621.6 0 55 50 57 621.6 0 57 51 82 621.6 0 82 52 126 621.6 0 126 53 78 621.6 0 78 54 85 621.6 0 85 55 60 621.6 0 60 56 69 621.6 0 69 57 140 621.6 0 140 58 92 621.6 0 92 59 84 621.6 0 84 60 66 621.6 0 66 61 93 621.6 0 93 62 82 621.6 0 82 63 104 621.6 0 104 64 132 621.6 0 132 65 212 621.6 0 212 66 237 621.6 0 237 67 623 621.6 0 623 68 1525 621.6 0 1525 69 21673 621.6 0 21673 70 4176 621.6 0 4176 71 6219 621.6 0 6219 72 75 621.6 0 75 73 53 621.6 0 53 74 56 621.6 0 56 75 59 621.6 0 59 76 60 621.6 0 60 77 58 621.6 0 58 78 68 621.6 0 68 79 59 621.6 0 59 80 122 621.6 0 122 81 78 621.6 0 78 82 109 621.6 0 109 83 84 621.6 0 84 84 68 621.6 0 68 85 65 621.6 0 65 86 79 621.6 0 79 87 97 621.6 0 97 88 103 621.6 0 103 89 100 621.6 0 100 90 83 621.6 0 83 91 83 621.6 0 83 92 83 621.6 0 83 93 90 621.6 0 90 94 102 621.6 0 102 95 94 621.6 0 94 96 67 621.6 0 67 97 95 621.6 0 95 98 85 621.6 0 85 99 69 621.6 0 69 100 179 621.6 0 179 101 248 621.6 0 248 102 358 621.6 0 358 103 77 621.6 0 77 104 66 621.6 0 66 105 74 621.6 0 74 106 72 621.6 0 72 107 79 621.6 0 79 108 75 621.6 0 75 109 86 621.6 0 86 110 80 621.6 0 80 111 86 621.6 0 86 112 61 621.6 0 61 113 81 621.6 0 81 114 77 621.6 0 77 115 80 621.6 0 80 116 92 621.6 0 92 117 86 621.6 0 86 118 96 621.6 0 96 119 115 621.6 0 115 120 97 621.6 0 97 121 70 621.6 0 70 122 71 621.6 0 71 123 62 621.6 0 62 124 89 621.6 0 89 125 76 621.6 0 76 126 74 621.6 0 74 127 86 621.6 0 86 128 67 621.6 0 67 129 73 621.6 0 73 130 104 621.6 0 104 131 98 621.6 0 98 132 233 621.6 0 233 133 222 621.6 0 222 134 294 621.6 0 294 135 144 621.6 0 144 136 182 621.6 0 182 137 195 621.6 0 195 138 178 621.6 0 178 139 110 621.6 0 110 140 75 621.6 0 75 141 55 621.6 0 55 142 76 621.6 0 76 143 70 621.6 0 70 144 78 621.6 0 78 145 92 621.6 0 92 146 71 621.6 0 71 147 92 621.6 0 92 148 89 621.6 0 89 149 69 621.6 0 69 150 100 621.6 0 100 151 105 621.6 0 105 152 95 621.6 0 95 153 87 621.6 0 87 154 108 621.6 0 108 155 68 621.6 0 68 156 92 621.6 0 92 157 115 621.6 0 115 158 116 621.6 0 116 159 399 621.6 0 399 160 120 621.6 0 120 161 121 621.6 0 121 162 86 621.6 0 86 163 119 621.6 0 119 164 82 621.6 0 82 165 67 621.6 0 67 166 102 621.6 0 102 167 197 621.6 0 197 168 134 621.6 0 134 169 112 621.6 0 112 170 99 621.6 0 99 171 104 621.6 0 104 172 88 621.6 0 88 173 103 621.6 0 103 174 77 621.6 0 77 175 100 621.6 0 100 176 169 621.6 0 169 177 294 621.6 0 294 178 326 621.6 0 326 179 422 621.6 0 422 180 214 621.6 0 214 181 273 621.6 0 273 182 242 621.6 0 242 183 174 621.6 0 174 184 334 621.6 0 334 185 340 621.6 0 340 186 102 621.6 0 102 187 113 621.6 0 113 188 110 621.6 0 110 189 124 621.6 0 124 190 120 621.6 0 120 191 118 621.6 0 118 192 76 621.6 0 76 193 102 621.6 0 102 194 83 621.6 0 83 195 79 621.6 0 79 196 88 621.6 0 88 197 99 621.6 0 99 198 101 621.6 0 101 199 116 621.6 0 116 200 109 621.6 0 109 201 84 621.6 0 84 202 352 621.6 0 352 203 339 621.6 0 339 204 305 621.6 0 305 205 180 621.6 0 180 206 86 621.6 0 86 207 82 621.6 0 82 208 88 621.6 0 88 209 86 621.6 0 86 210 110 621.6 0 110 211 119 621.6 0 119 212 100 621.6 0 100 213 146 621.6 0 146 214 84 621.6 0 84 215 84 621.6 0 84 216 93 621.6 0 93 217 98 621.6 0 98 218 75 621.6 0 75 219 80 621.6 0 80 220 95 621.6 0 95 221 97 621.6 0 97 222 135 621.6 0 135 223 188 621.6 0 188 224 193 621.6 0 193 225 151 621.6 0 151 226 118 621.6 0 118 227 347 621.6 0 347 228 176 621.6 0 176 229 229 621.6 0 229 230 84 621.6 0 84 231 91 621.6 0 91 232 84 621.6 0 84 233 89 621.6 0 89 234 82 621.6 0 82 235 94 621.6 0 94 236 63 621.6 0 63 237 73 621.6 0 73 238 97 621.6 0 97 239 89 621.6 0 89 240 79 621.6 0 79 241 77 621.6 0 77 242 162 621.6 0 162 243 194 621.6 0 194 244 186 621.6 0 186 245 122 621.6 0 122 246 129 621.6 0 129 247 82 621.6 0 82 248 71 621.6 0 71 249 76 621.6 0 76 250 128 621.6 0 128 251 121 621.6 0 121 252 105 621.6 0 105 253 105 621.6 0 105 254 105 621.6 0 105 255 129 621.6 0 129 256 165 621.6 0 165 257 147 621.6 0 147 258 109 621.6 0 109 259 103 621.6 0 103 260 101 621.6 0 101 261 86 621.6 0 86 262 98 621.6 0 98 263 123 621.6 0 123 264 121 621.6 0 121 265 168 621.6 0 168 266 163 621.6 0 163 267 160 621.6 0 160 268 158 621.6 0 158 269 1634 621.6 0 1634 270 426 621.6 0 426 271 1106 621.6 0 1106 272 164 621.6 0 164 273 160 621.6 0 160 274 138 621.6 0 138 275 119 621.6 0 119 276 172 621.6 0 172 277 220 621.6 0 220 278 168 621.6 0 168 279 130 621.6 0 130 280 87 621.6 0 87 281 115 621.6 0 115 282 85 621.6 0 85 283 109 621.6 0 109 284 129 621.6 0 129 285 117 621.6 0 117 286 116 621.6 0 116 287 113 621.6 0 113 288 117 621.6 0 117 289 87 621.6 0 87 290 107 621.6 0 107 291 205 621.6 0 205 292 179 621.6 0 179 293 165 621.6 0 165 294 123 621.6 0 123 295 85 621.6 0 85 296 102 621.6 0 102 297 98 621.6 0 98 298 111 621.6 0 111 299 170 621.6 0 170 300 150 621.6 0 150 301 128 621.6 0 128 Finished in 11.69 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 238,285 Reads with adapters: 97,171 (40.8%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 97,147 (40.8%) Total basepairs processed: 65,414,197 bp Total written (filtered): 26,366,161 bp (40.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 97171 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 39 0.0 3 0 0 39 19 1405 0.0 3 0 1375 24 4 2 20 94803 0.0 4 93231 1507 59 3 3 21 194 0.0 4 33 154 7 22 15 0.0 4 4 0 10 0 1 23 83 0.0 4 5 0 0 71 7 24 5 0.0 4 0 0 0 0 5 26 1 0.0 4 0 1 28 1 0.0 4 0 0 1 38 1 0.0 4 1 65 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 8 0.0 4 0 0 0 0 8 127 573 0.0 4 0 0 0 0 573 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 1 135 5 0.0 4 0 0 0 0 5 138 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 209 1 0.0 4 1 220 1 0.0 4 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 282 8 0.0 4 0 0 0 0 8 283 5 0.0 4 0 0 0 0 5 291 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.52 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 97,147 Reads with adapters: 17,069 (17.6%) Reads written (passing filters): 97,147 (100.0%) Total basepairs processed: 26,366,161 bp Total written (filtered): 26,198,496 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 17069 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.4% G: 95.6% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 42 1517.9 0 42 4 20 379.5 0 20 5 4 94.9 0 4 6 1 23.7 0 1 7 3 5.9 0 3 8 3 1.5 0 3 9 10529 0.4 0 310 10219 10 5726 0.1 1 214 5512 11 679 0.0 1 4 675 12 12 0.0 1 0 12 13 7 0.0 1 0 7 23 2 0.0 1 1 1 40 2 0.0 1 2 108 1 0.0 1 1 149 1 0.0 1 1 150 6 0.0 1 1 5 152 1 0.0 1 0 1 169 9 0.0 1 8 1 195 2 0.0 1 2 207 1 0.0 1 1 208 8 0.0 1 7 1 209 1 0.0 1 0 1 210 1 0.0 1 1 228 1 0.0 1 0 1 257 7 0.0 1 6 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.