This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.62 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 105,646 (4.8%) Reads that were too short: 14,434 (0.7%) Reads written (passing filters): 91,212 (4.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 24,688,272 bp (3.7%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 105646 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1174 535.2 0 1174 7 31283 535.2 0 31283 8 1652 535.2 0 1652 9 35672 535.2 0 35672 10 168 535.2 0 168 11 84 535.2 0 84 12 80 535.2 0 80 13 75 535.2 0 75 14 71 535.2 0 71 15 86 535.2 0 86 16 78 535.2 0 78 17 67 535.2 0 67 18 67 535.2 0 67 19 69 535.2 0 69 20 91 535.2 0 91 21 91 535.2 0 91 22 159 535.2 0 159 23 367 535.2 0 367 24 354 535.2 0 354 25 396 535.2 0 396 26 57 535.2 0 57 27 78 535.2 0 78 28 77 535.2 0 77 29 80 535.2 0 80 30 61 535.2 0 61 31 60 535.2 0 60 32 51 535.2 0 51 33 83 535.2 0 83 34 103 535.2 0 103 35 114 535.2 0 114 36 83 535.2 0 83 37 69 535.2 0 69 38 65 535.2 0 65 39 82 535.2 0 82 40 58 535.2 0 58 41 60 535.2 0 60 42 57 535.2 0 57 43 72 535.2 0 72 44 62 535.2 0 62 45 77 535.2 0 77 46 55 535.2 0 55 47 71 535.2 0 71 48 69 535.2 0 69 49 55 535.2 0 55 50 71 535.2 0 71 51 55 535.2 0 55 52 60 535.2 0 60 53 49 535.2 0 49 54 58 535.2 0 58 55 80 535.2 0 80 56 77 535.2 0 77 57 67 535.2 0 67 58 58 535.2 0 58 59 51 535.2 0 51 60 81 535.2 0 81 61 71 535.2 0 71 62 37 535.2 0 37 63 72 535.2 0 72 64 68 535.2 0 68 65 71 535.2 0 71 66 65 535.2 0 65 67 50 535.2 0 50 68 69 535.2 0 69 69 94 535.2 0 94 70 79 535.2 0 79 71 83 535.2 0 83 72 35 535.2 0 35 73 93 535.2 0 93 74 107 535.2 0 107 75 56 535.2 0 56 76 58 535.2 0 58 77 42 535.2 0 42 78 71 535.2 0 71 79 47 535.2 0 47 80 49 535.2 0 49 81 58 535.2 0 58 82 66 535.2 0 66 83 64 535.2 0 64 84 79 535.2 0 79 85 73 535.2 0 73 86 96 535.2 0 96 87 93 535.2 0 93 88 719 535.2 0 719 89 594 535.2 0 594 90 546 535.2 0 546 91 39 535.2 0 39 92 52 535.2 0 52 93 47 535.2 0 47 94 64 535.2 0 64 95 70 535.2 0 70 96 236 535.2 0 236 97 263 535.2 0 263 98 174 535.2 0 174 99 95 535.2 0 95 100 142 535.2 0 142 101 646 535.2 0 646 102 686 535.2 0 686 103 642 535.2 0 642 104 86 535.2 0 86 105 72 535.2 0 72 106 66 535.2 0 66 107 43 535.2 0 43 108 44 535.2 0 44 109 50 535.2 0 50 110 81 535.2 0 81 111 82 535.2 0 82 112 66 535.2 0 66 113 84 535.2 0 84 114 82 535.2 0 82 115 87 535.2 0 87 116 56 535.2 0 56 117 84 535.2 0 84 118 67 535.2 0 67 119 69 535.2 0 69 120 69 535.2 0 69 121 74 535.2 0 74 122 73 535.2 0 73 123 69 535.2 0 69 124 71 535.2 0 71 125 94 535.2 0 94 126 179 535.2 0 179 127 1036 535.2 0 1036 128 1005 535.2 0 1005 129 877 535.2 0 877 130 84 535.2 0 84 131 158 535.2 0 158 132 100 535.2 0 100 133 86 535.2 0 86 134 69 535.2 0 69 135 102 535.2 0 102 136 73 535.2 0 73 137 51 535.2 0 51 138 66 535.2 0 66 139 53 535.2 0 53 140 70 535.2 0 70 141 86 535.2 0 86 142 56 535.2 0 56 143 57 535.2 0 57 144 46 535.2 0 46 145 81 535.2 0 81 146 71 535.2 0 71 147 86 535.2 0 86 148 138 535.2 0 138 149 95 535.2 0 95 150 53 535.2 0 53 151 61 535.2 0 61 152 53 535.2 0 53 153 64 535.2 0 64 154 50 535.2 0 50 155 50 535.2 0 50 156 67 535.2 0 67 157 53 535.2 0 53 158 60 535.2 0 60 159 54 535.2 0 54 160 53 535.2 0 53 161 66 535.2 0 66 162 57 535.2 0 57 163 53 535.2 0 53 164 42 535.2 0 42 165 54 535.2 0 54 166 55 535.2 0 55 167 73 535.2 0 73 168 78 535.2 0 78 169 86 535.2 0 86 170 63 535.2 0 63 171 56 535.2 0 56 172 51 535.2 0 51 173 41 535.2 0 41 174 34 535.2 0 34 175 37 535.2 0 37 176 88 535.2 0 88 177 73 535.2 0 73 178 67 535.2 0 67 179 38 535.2 0 38 180 39 535.2 0 39 181 52 535.2 0 52 182 114 535.2 0 114 183 96 535.2 0 96 184 63 535.2 0 63 185 54 535.2 0 54 186 55 535.2 0 55 187 52 535.2 0 52 188 47 535.2 0 47 189 75 535.2 0 75 190 54 535.2 0 54 191 34 535.2 0 34 192 49 535.2 0 49 193 80 535.2 0 80 194 80 535.2 0 80 195 232 535.2 0 232 196 154 535.2 0 154 197 98 535.2 0 98 198 78 535.2 0 78 199 71 535.2 0 71 200 121 535.2 0 121 201 139 535.2 0 139 202 174 535.2 0 174 203 145 535.2 0 145 204 86 535.2 0 86 205 89 535.2 0 89 206 75 535.2 0 75 207 92 535.2 0 92 208 113 535.2 0 113 209 164 535.2 0 164 210 123 535.2 0 123 211 60 535.2 0 60 212 59 535.2 0 59 213 69 535.2 0 69 214 107 535.2 0 107 215 100 535.2 0 100 216 84 535.2 0 84 217 73 535.2 0 73 218 57 535.2 0 57 219 55 535.2 0 55 220 47 535.2 0 47 221 75 535.2 0 75 222 76 535.2 0 76 223 94 535.2 0 94 224 84 535.2 0 84 225 65 535.2 0 65 226 66 535.2 0 66 227 67 535.2 0 67 228 46 535.2 0 46 229 76 535.2 0 76 230 82 535.2 0 82 231 95 535.2 0 95 232 66 535.2 0 66 233 89 535.2 0 89 234 117 535.2 0 117 235 172 535.2 0 172 236 192 535.2 0 192 237 98 535.2 0 98 238 80 535.2 0 80 239 155 535.2 0 155 240 207 535.2 0 207 241 234 535.2 0 234 242 168 535.2 0 168 243 101 535.2 0 101 244 51 535.2 0 51 245 69 535.2 0 69 246 58 535.2 0 58 247 63 535.2 0 63 248 84 535.2 0 84 249 79 535.2 0 79 250 86 535.2 0 86 251 74 535.2 0 74 252 67 535.2 0 67 253 91 535.2 0 91 254 211 535.2 0 211 255 186 535.2 0 186 256 216 535.2 0 216 257 136 535.2 0 136 258 99 535.2 0 99 259 667 535.2 0 667 260 1490 535.2 0 1490 261 1143 535.2 0 1143 262 464 535.2 0 464 263 102 535.2 0 102 264 37 535.2 0 37 265 54 535.2 0 54 266 71 535.2 0 71 267 63 535.2 0 63 268 78 535.2 0 78 269 63 535.2 0 63 270 53 535.2 0 53 271 54 535.2 0 54 272 57 535.2 0 57 273 66 535.2 0 66 274 64 535.2 0 64 275 69 535.2 0 69 276 85 535.2 0 85 277 68 535.2 0 68 278 88 535.2 0 88 279 570 535.2 0 570 280 732 535.2 0 732 281 626 535.2 0 626 282 58 535.2 0 58 283 71 535.2 0 71 284 81 535.2 0 81 285 55 535.2 0 55 286 69 535.2 0 69 287 51 535.2 0 51 288 68 535.2 0 68 289 59 535.2 0 59 290 73 535.2 0 73 291 90 535.2 0 90 292 133 535.2 0 133 293 152 535.2 0 152 294 189 535.2 0 189 295 135 535.2 0 135 296 119 535.2 0 119 297 99 535.2 0 99 298 78 535.2 0 78 299 90 535.2 0 90 300 69 535.2 0 69 301 84 535.2 0 84 Finished in 4.22 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 91,212 Reads with adapters: 37,881 (41.5%) Reads that were too short: 73 (0.1%) Reads written (passing filters): 37,808 (41.5%) Total basepairs processed: 24,688,272 bp Total written (filtered): 10,372,217 bp (42.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 37881 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 14 0.0 2 0 0 0 14 15 28 0.0 3 0 0 19 9 16 542 0.0 3 0 462 58 22 17 36750 0.0 3 33269 2751 601 129 18 87 0.0 3 3 60 14 10 19 128 0.0 3 0 0 68 60 20 73 0.0 3 15 5 7 46 21 3 0.0 3 0 0 1 2 22 2 0.0 3 0 1 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 4 0.0 3 0 0 1 3 38 1 0.0 3 0 0 0 1 40 4 0.0 3 0 0 4 42 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 71 0.0 3 0 0 0 71 66 9 0.0 3 0 0 0 9 67 8 0.0 3 0 0 0 8 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 75 10 0.0 3 0 0 0 10 77 2 0.0 3 0 0 0 2 78 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 1 96 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 1 103 12 0.0 3 0 0 0 12 104 8 0.0 3 0 0 0 8 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 115 4 0.0 3 0 0 0 4 121 1 0.0 3 1 123 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 155 3 0.0 3 0 0 0 3 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 165 3 0.0 3 0 0 0 3 166 11 0.0 3 0 0 0 11 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 1 2 188 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 194 5 0.0 3 0 0 1 4 198 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 7 0.0 3 0 0 0 7 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 285 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.32 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 37,808 Reads with adapters: 201 (0.5%) Reads written (passing filters): 37,808 (100.0%) Total basepairs processed: 10,372,217 bp Total written (filtered): 10,371,577 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 201 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.3% C: 12.4% G: 36.8% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 168 590.8 0 168 4 29 147.7 0 29 5 4 36.9 0 4