This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.74 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 95,560 (4.4%) Reads that were too short: 8,546 (0.4%) Reads written (passing filters): 87,014 (4.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 24,324,564 bp (3.7%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 95560 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1109 535.2 0 1109 7 39469 535.2 0 39469 8 911 535.2 0 911 9 32959 535.2 0 32959 10 84 535.2 0 84 11 53 535.2 0 53 12 28 535.2 0 28 13 38 535.2 0 38 14 42 535.2 0 42 15 39 535.2 0 39 16 31 535.2 0 31 17 53 535.2 0 53 18 54 535.2 0 54 19 44 535.2 0 44 20 51 535.2 0 51 21 45 535.2 0 45 22 52 535.2 0 52 23 375 535.2 0 375 24 267 535.2 0 267 25 442 535.2 0 442 26 38 535.2 0 38 27 17 535.2 0 17 28 34 535.2 0 34 29 23 535.2 0 23 30 31 535.2 0 31 31 27 535.2 0 27 32 34 535.2 0 34 33 42 535.2 0 42 34 29 535.2 0 29 35 32 535.2 0 32 36 29 535.2 0 29 37 26 535.2 0 26 38 30 535.2 0 30 39 36 535.2 0 36 40 24 535.2 0 24 41 28 535.2 0 28 42 24 535.2 0 24 43 43 535.2 0 43 44 36 535.2 0 36 45 27 535.2 0 27 46 36 535.2 0 36 47 34 535.2 0 34 48 20 535.2 0 20 49 25 535.2 0 25 50 28 535.2 0 28 51 36 535.2 0 36 52 21 535.2 0 21 53 20 535.2 0 20 54 25 535.2 0 25 55 30 535.2 0 30 56 29 535.2 0 29 57 26 535.2 0 26 58 21 535.2 0 21 59 18 535.2 0 18 60 33 535.2 0 33 61 28 535.2 0 28 62 20 535.2 0 20 63 28 535.2 0 28 64 32 535.2 0 32 65 13 535.2 0 13 66 23 535.2 0 23 67 15 535.2 0 15 68 24 535.2 0 24 69 30 535.2 0 30 70 31 535.2 0 31 71 31 535.2 0 31 72 17 535.2 0 17 73 44 535.2 0 44 74 62 535.2 0 62 75 27 535.2 0 27 76 31 535.2 0 31 77 26 535.2 0 26 78 37 535.2 0 37 79 17 535.2 0 17 80 17 535.2 0 17 81 25 535.2 0 25 82 30 535.2 0 30 83 29 535.2 0 29 84 51 535.2 0 51 85 23 535.2 0 23 86 48 535.2 0 48 87 53 535.2 0 53 88 453 535.2 0 453 89 314 535.2 0 314 90 317 535.2 0 317 91 23 535.2 0 23 92 26 535.2 0 26 93 20 535.2 0 20 94 29 535.2 0 29 95 26 535.2 0 26 96 47 535.2 0 47 97 49 535.2 0 49 98 51 535.2 0 51 99 54 535.2 0 54 100 45 535.2 0 45 101 278 535.2 0 278 102 526 535.2 0 526 103 530 535.2 0 530 104 45 535.2 0 45 105 36 535.2 0 36 106 32 535.2 0 32 107 15 535.2 0 15 108 20 535.2 0 20 109 24 535.2 0 24 110 32 535.2 0 32 111 31 535.2 0 31 112 31 535.2 0 31 113 41 535.2 0 41 114 48 535.2 0 48 115 19 535.2 0 19 116 29 535.2 0 29 117 44 535.2 0 44 118 17 535.2 0 17 119 39 535.2 0 39 120 41 535.2 0 41 121 40 535.2 0 40 122 49 535.2 0 49 123 32 535.2 0 32 124 53 535.2 0 53 125 60 535.2 0 60 126 181 535.2 0 181 127 1013 535.2 0 1013 128 1063 535.2 0 1063 129 972 535.2 0 972 130 35 535.2 0 35 131 112 535.2 0 112 132 45 535.2 0 45 133 56 535.2 0 56 134 18 535.2 0 18 135 54 535.2 0 54 136 27 535.2 0 27 137 17 535.2 0 17 138 20 535.2 0 20 139 25 535.2 0 25 140 26 535.2 0 26 141 34 535.2 0 34 142 24 535.2 0 24 143 32 535.2 0 32 144 22 535.2 0 22 145 42 535.2 0 42 146 22 535.2 0 22 147 24 535.2 0 24 148 104 535.2 0 104 149 48 535.2 0 48 150 23 535.2 0 23 151 26 535.2 0 26 152 23 535.2 0 23 153 25 535.2 0 25 154 27 535.2 0 27 155 34 535.2 0 34 156 30 535.2 0 30 157 25 535.2 0 25 158 31 535.2 0 31 159 29 535.2 0 29 160 27 535.2 0 27 161 33 535.2 0 33 162 29 535.2 0 29 163 24 535.2 0 24 164 15 535.2 0 15 165 26 535.2 0 26 166 15 535.2 0 15 167 29 535.2 0 29 168 25 535.2 0 25 169 33 535.2 0 33 170 24 535.2 0 24 171 28 535.2 0 28 172 21 535.2 0 21 173 14 535.2 0 14 174 15 535.2 0 15 175 18 535.2 0 18 176 25 535.2 0 25 177 32 535.2 0 32 178 34 535.2 0 34 179 16 535.2 0 16 180 23 535.2 0 23 181 25 535.2 0 25 182 27 535.2 0 27 183 27 535.2 0 27 184 24 535.2 0 24 185 29 535.2 0 29 186 22 535.2 0 22 187 34 535.2 0 34 188 34 535.2 0 34 189 31 535.2 0 31 190 29 535.2 0 29 191 20 535.2 0 20 192 19 535.2 0 19 193 44 535.2 0 44 194 29 535.2 0 29 195 42 535.2 0 42 196 39 535.2 0 39 197 37 535.2 0 37 198 28 535.2 0 28 199 22 535.2 0 22 200 52 535.2 0 52 201 69 535.2 0 69 202 56 535.2 0 56 203 39 535.2 0 39 204 34 535.2 0 34 205 43 535.2 0 43 206 24 535.2 0 24 207 29 535.2 0 29 208 52 535.2 0 52 209 88 535.2 0 88 210 32 535.2 0 32 211 27 535.2 0 27 212 25 535.2 0 25 213 40 535.2 0 40 214 38 535.2 0 38 215 41 535.2 0 41 216 43 535.2 0 43 217 27 535.2 0 27 218 27 535.2 0 27 219 36 535.2 0 36 220 29 535.2 0 29 221 29 535.2 0 29 222 25 535.2 0 25 223 25 535.2 0 25 224 33 535.2 0 33 225 36 535.2 0 36 226 27 535.2 0 27 227 23 535.2 0 23 228 27 535.2 0 27 229 41 535.2 0 41 230 43 535.2 0 43 231 33 535.2 0 33 232 34 535.2 0 34 233 23 535.2 0 23 234 49 535.2 0 49 235 69 535.2 0 69 236 96 535.2 0 96 237 43 535.2 0 43 238 47 535.2 0 47 239 56 535.2 0 56 240 71 535.2 0 71 241 58 535.2 0 58 242 45 535.2 0 45 243 43 535.2 0 43 244 46 535.2 0 46 245 49 535.2 0 49 246 43 535.2 0 43 247 34 535.2 0 34 248 50 535.2 0 50 249 63 535.2 0 63 250 47 535.2 0 47 251 47 535.2 0 47 252 30 535.2 0 30 253 54 535.2 0 54 254 228 535.2 0 228 255 218 535.2 0 218 256 174 535.2 0 174 257 81 535.2 0 81 258 55 535.2 0 55 259 404 535.2 0 404 260 1068 535.2 0 1068 261 625 535.2 0 625 262 384 535.2 0 384 263 39 535.2 0 39 264 29 535.2 0 29 265 26 535.2 0 26 266 32 535.2 0 32 267 21 535.2 0 21 268 31 535.2 0 31 269 27 535.2 0 27 270 32 535.2 0 32 271 20 535.2 0 20 272 33 535.2 0 33 273 40 535.2 0 40 274 32 535.2 0 32 275 37 535.2 0 37 276 51 535.2 0 51 277 51 535.2 0 51 278 48 535.2 0 48 279 587 535.2 0 587 280 619 535.2 0 619 281 409 535.2 0 409 282 49 535.2 0 49 283 35 535.2 0 35 284 32 535.2 0 32 285 29 535.2 0 29 286 37 535.2 0 37 287 30 535.2 0 30 288 41 535.2 0 41 289 23 535.2 0 23 290 34 535.2 0 34 291 38 535.2 0 38 292 61 535.2 0 61 293 59 535.2 0 59 294 121 535.2 0 121 295 77 535.2 0 77 296 89 535.2 0 89 297 75 535.2 0 75 298 42 535.2 0 42 299 26 535.2 0 26 300 46 535.2 0 46 301 32 535.2 0 32 Finished in 4.57 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 87,014 Reads with adapters: 40,253 (46.3%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 40,241 (46.2%) Total basepairs processed: 24,324,564 bp Total written (filtered): 11,004,781 bp (45.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 40253 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 18 0.0 3 0 0 18 19 419 0.0 3 0 412 7 20 39239 0.0 4 38585 620 28 5 1 21 109 0.0 4 17 87 4 0 1 22 8 0.0 4 1 1 5 1 23 250 0.0 4 232 3 0 12 3 33 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 1 58 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 186 0.0 4 0 0 0 0 186 139 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 183 1 0.0 4 1 193 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 211 1 0.0 4 1 214 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 1 0.0 4 1 229 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.40 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 40,241 Reads with adapters: 2,381 (5.9%) Reads written (passing filters): 40,241 (100.0%) Total basepairs processed: 11,004,781 bp Total written (filtered): 10,970,298 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2381 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 3.0% G: 91.9% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 57 628.8 0 57 4 11 157.2 0 11 5 2 39.3 0 2 8 1 0.6 0 1 9 28 0.2 0 1 27 10 32 0.0 1 1 31 11 2137 0.0 1 17 2120 12 65 0.0 1 7 58 13 1 0.0 1 0 1 15 2 0.0 1 0 2 20 1 0.0 1 1 24 1 0.0 1 1 125 1 0.0 1 1 140 1 0.0 1 1 152 1 0.0 1 0 1 171 1 0.0 1 1 179 1 0.0 1 0 1 191 1 0.0 1 0 1 210 1 0.0 1 1 225 35 0.0 1 33 2 261 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.