This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.56 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 288,260 (13.1%) Reads that were too short: 12,751 (0.6%) Reads written (passing filters): 275,509 (12.6%) Total basepairs processed: 659,880,795 bp Total written (filtered): 79,192,779 bp (12.0%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 288260 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1774 535.2 0 1774 7 5494 535.2 0 5494 8 143308 535.2 0 143308 9 110941 535.2 0 110941 10 310 535.2 0 310 11 39 535.2 0 39 12 13 535.2 0 13 13 22 535.2 0 22 14 23 535.2 0 23 15 80 535.2 0 80 16 38 535.2 0 38 17 55 535.2 0 55 18 71 535.2 0 71 19 86 535.2 0 86 20 63 535.2 0 63 21 22 535.2 0 22 22 31 535.2 0 31 23 38 535.2 0 38 24 30 535.2 0 30 25 30 535.2 0 30 26 29 535.2 0 29 27 31 535.2 0 31 28 69 535.2 0 69 29 165 535.2 0 165 30 86 535.2 0 86 31 23 535.2 0 23 32 31 535.2 0 31 33 22 535.2 0 22 34 43 535.2 0 43 35 34 535.2 0 34 36 24 535.2 0 24 37 51 535.2 0 51 38 21 535.2 0 21 39 19 535.2 0 19 40 31 535.2 0 31 41 25 535.2 0 25 42 39 535.2 0 39 43 83 535.2 0 83 44 101 535.2 0 101 45 453 535.2 0 453 46 432 535.2 0 432 47 406 535.2 0 406 48 26 535.2 0 26 49 25 535.2 0 25 50 20 535.2 0 20 51 13 535.2 0 13 52 22 535.2 0 22 53 17 535.2 0 17 54 19 535.2 0 19 55 11 535.2 0 11 56 19 535.2 0 19 57 20 535.2 0 20 58 15 535.2 0 15 59 17 535.2 0 17 60 18 535.2 0 18 61 17 535.2 0 17 62 19 535.2 0 19 63 16 535.2 0 16 64 23 535.2 0 23 65 17 535.2 0 17 66 20 535.2 0 20 67 20 535.2 0 20 68 81 535.2 0 81 69 32 535.2 0 32 70 48 535.2 0 48 71 99 535.2 0 99 72 109 535.2 0 109 73 127 535.2 0 127 74 25 535.2 0 25 75 45 535.2 0 45 76 19 535.2 0 19 77 32 535.2 0 32 78 23 535.2 0 23 79 43 535.2 0 43 80 87 535.2 0 87 81 75 535.2 0 75 82 54 535.2 0 54 83 54 535.2 0 54 84 71 535.2 0 71 85 68 535.2 0 68 86 29 535.2 0 29 87 15 535.2 0 15 88 29 535.2 0 29 89 22 535.2 0 22 90 25 535.2 0 25 91 21 535.2 0 21 92 35 535.2 0 35 93 31 535.2 0 31 94 39 535.2 0 39 95 426 535.2 0 426 96 209 535.2 0 209 97 228 535.2 0 228 98 19 535.2 0 19 99 20 535.2 0 20 100 20 535.2 0 20 101 34 535.2 0 34 102 21 535.2 0 21 103 33 535.2 0 33 104 28 535.2 0 28 105 14 535.2 0 14 106 21 535.2 0 21 107 29 535.2 0 29 108 57 535.2 0 57 109 17 535.2 0 17 110 25 535.2 0 25 111 34 535.2 0 34 112 45 535.2 0 45 113 305 535.2 0 305 114 370 535.2 0 370 115 306 535.2 0 306 116 199 535.2 0 199 117 37 535.2 0 37 118 13 535.2 0 13 119 27 535.2 0 27 120 34 535.2 0 34 121 38 535.2 0 38 122 53 535.2 0 53 123 46 535.2 0 46 124 61 535.2 0 61 125 115 535.2 0 115 126 29 535.2 0 29 127 30 535.2 0 30 128 45 535.2 0 45 129 43 535.2 0 43 130 38 535.2 0 38 131 33 535.2 0 33 132 32 535.2 0 32 133 49 535.2 0 49 134 93 535.2 0 93 135 222 535.2 0 222 136 168 535.2 0 168 137 338 535.2 0 338 138 342 535.2 0 342 139 744 535.2 0 744 140 90 535.2 0 90 141 22 535.2 0 22 142 66 535.2 0 66 143 76 535.2 0 76 144 105 535.2 0 105 145 54 535.2 0 54 146 51 535.2 0 51 147 25 535.2 0 25 148 24 535.2 0 24 149 40 535.2 0 40 150 45 535.2 0 45 151 35 535.2 0 35 152 20 535.2 0 20 153 29 535.2 0 29 154 28 535.2 0 28 155 29 535.2 0 29 156 30 535.2 0 30 157 25 535.2 0 25 158 37 535.2 0 37 159 24 535.2 0 24 160 21 535.2 0 21 161 12 535.2 0 12 162 53 535.2 0 53 163 64 535.2 0 64 164 110 535.2 0 110 165 45 535.2 0 45 166 26 535.2 0 26 167 133 535.2 0 133 168 198 535.2 0 198 169 326 535.2 0 326 170 100 535.2 0 100 171 26 535.2 0 26 172 50 535.2 0 50 173 30 535.2 0 30 174 38 535.2 0 38 175 30 535.2 0 30 176 13 535.2 0 13 177 38 535.2 0 38 178 32 535.2 0 32 179 73 535.2 0 73 180 102 535.2 0 102 181 97 535.2 0 97 182 45 535.2 0 45 183 64 535.2 0 64 184 221 535.2 0 221 185 250 535.2 0 250 186 307 535.2 0 307 187 94 535.2 0 94 188 47 535.2 0 47 189 40 535.2 0 40 190 51 535.2 0 51 191 43 535.2 0 43 192 26 535.2 0 26 193 33 535.2 0 33 194 19 535.2 0 19 195 21 535.2 0 21 196 23 535.2 0 23 197 31 535.2 0 31 198 52 535.2 0 52 199 65 535.2 0 65 200 45 535.2 0 45 201 72 535.2 0 72 202 55 535.2 0 55 203 60 535.2 0 60 204 184 535.2 0 184 205 270 535.2 0 270 206 377 535.2 0 377 207 85 535.2 0 85 208 75 535.2 0 75 209 76 535.2 0 76 210 94 535.2 0 94 211 98 535.2 0 98 212 71 535.2 0 71 213 54 535.2 0 54 214 70 535.2 0 70 215 86 535.2 0 86 216 114 535.2 0 114 217 70 535.2 0 70 218 57 535.2 0 57 219 63 535.2 0 63 220 67 535.2 0 67 221 76 535.2 0 76 222 82 535.2 0 82 223 156 535.2 0 156 224 217 535.2 0 217 225 2315 535.2 0 2315 226 1932 535.2 0 1932 227 2181 535.2 0 2181 228 447 535.2 0 447 229 46 535.2 0 46 230 34 535.2 0 34 231 39 535.2 0 39 232 46 535.2 0 46 233 43 535.2 0 43 234 50 535.2 0 50 235 76 535.2 0 76 236 102 535.2 0 102 237 60 535.2 0 60 238 37 535.2 0 37 239 34 535.2 0 34 240 37 535.2 0 37 241 80 535.2 0 80 242 31 535.2 0 31 243 21 535.2 0 21 244 61 535.2 0 61 245 45 535.2 0 45 246 54 535.2 0 54 247 58 535.2 0 58 248 52 535.2 0 52 249 39 535.2 0 39 250 41 535.2 0 41 251 23 535.2 0 23 252 18 535.2 0 18 253 30 535.2 0 30 254 45 535.2 0 45 255 40 535.2 0 40 256 22 535.2 0 22 257 56 535.2 0 56 258 39 535.2 0 39 259 47 535.2 0 47 260 43 535.2 0 43 261 35 535.2 0 35 262 19 535.2 0 19 263 28 535.2 0 28 264 59 535.2 0 59 265 83 535.2 0 83 266 84 535.2 0 84 267 28 535.2 0 28 268 27 535.2 0 27 269 34 535.2 0 34 270 46 535.2 0 46 271 44 535.2 0 44 272 38 535.2 0 38 273 26 535.2 0 26 274 42 535.2 0 42 275 63 535.2 0 63 276 50 535.2 0 50 277 28 535.2 0 28 278 53 535.2 0 53 279 44 535.2 0 44 280 61 535.2 0 61 281 39 535.2 0 39 282 43 535.2 0 43 283 31 535.2 0 31 284 30 535.2 0 30 285 36 535.2 0 36 286 22 535.2 0 22 287 34 535.2 0 34 288 29 535.2 0 29 289 43 535.2 0 43 290 38 535.2 0 38 291 54 535.2 0 54 292 37 535.2 0 37 293 51 535.2 0 51 294 40 535.2 0 40 295 57 535.2 0 57 296 48 535.2 0 48 297 81 535.2 0 81 298 75 535.2 0 75 299 62 535.2 0 62 300 32 535.2 0 32 301 96 535.2 0 96 Finished in 12.28 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 275,509 Reads with adapters: 139,771 (50.7%) Reads that were too short: 168 (0.1%) Reads written (passing filters): 139,603 (50.7%) Total basepairs processed: 79,192,779 bp Total written (filtered): 38,505,815 bp (48.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 139771 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 57 0.0 2 0 0 0 57 15 126 0.0 3 0 0 55 71 16 1419 0.0 3 0 1073 180 166 17 136492 0.0 3 123424 10264 2185 619 18 416 0.0 3 80 253 44 39 19 747 0.0 3 1 0 396 350 20 138 0.0 3 1 0 0 137 21 2 0.0 3 1 0 0 1 23 1 0.0 3 1 24 10 0.0 3 0 0 0 10 25 3 0.0 3 1 1 0 1 27 5 0.0 3 0 0 1 4 28 3 0.0 3 0 0 0 3 30 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 0 3 35 3 0.0 3 1 0 0 2 38 3 0.0 3 0 0 0 3 40 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 5 0.0 3 0 0 0 5 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 4 0.0 3 0 0 0 4 53 1 0.0 3 0 0 0 1 54 19 0.0 3 0 0 0 19 55 9 0.0 3 0 0 0 9 56 9 0.0 3 0 0 0 9 57 4 0.0 3 0 0 0 4 58 5 0.0 3 0 0 0 5 59 1 0.0 3 0 0 0 1 61 3 0.0 3 0 0 0 3 62 3 0.0 3 0 0 0 3 63 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 78 9 0.0 3 0 0 1 8 79 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 83 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 98 2 0.0 3 0 0 0 2 99 4 0.0 3 0 0 0 4 102 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 5 0.0 3 0 0 0 5 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 121 3 0.0 3 0 0 0 3 126 15 0.0 3 0 0 2 13 127 3 0.0 3 0 0 2 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 1 137 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 7 0.0 3 0 0 0 7 148 1 0.0 3 0 0 0 1 149 4 0.0 3 0 0 0 4 150 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 4 0.0 3 0 0 0 4 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 4 0.0 3 0 0 0 4 156 4 0.0 3 0 0 0 4 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 4 0.0 3 0 0 0 4 167 5 0.0 3 0 0 0 5 168 3 0.0 3 0 0 0 3 170 2 0.0 3 0 0 0 2 173 4 0.0 3 2 0 0 2 179 4 0.0 3 0 0 0 4 181 2 0.0 3 0 0 0 2 184 4 0.0 3 0 0 1 3 187 4 0.0 3 0 0 0 4 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 199 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 224 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 5 0.0 3 0 0 2 3 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 248 2 0.0 3 0 0 1 1 258 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 279 12 0.0 3 0 0 0 12 280 7 0.0 3 0 0 0 7 281 18 0.0 3 0 0 1 17 282 11 0.0 3 0 0 1 10 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 3 0.0 3 0 0 0 3 286 1 0.0 3 0 0 0 1 288 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.84 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 139,603 Reads with adapters: 308 (0.2%) Reads written (passing filters): 139,603 (100.0%) Total basepairs processed: 38,505,815 bp Total written (filtered): 38,504,766 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 308 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.9% C: 14.0% G: 32.5% T: 10.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 223 2181.3 0 223 4 54 545.3 0 54 5 27 136.3 0 27 6 3 34.1 0 3 11 1 0.0 1 0 1