This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.19 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 293,655 (13.4%) Reads that were too short: 8,018 (0.4%) Reads written (passing filters): 285,637 (13.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 82,623,961 bp (12.5%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 293655 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1342 535.2 0 1342 7 2910 535.2 0 2910 8 138214 535.2 0 138214 9 134147 535.2 0 134147 10 207 535.2 0 207 11 27 535.2 0 27 12 11 535.2 0 11 13 15 535.2 0 15 14 17 535.2 0 17 15 7 535.2 0 7 16 11 535.2 0 11 17 16 535.2 0 16 18 32 535.2 0 32 19 26 535.2 0 26 20 23 535.2 0 23 21 16 535.2 0 16 22 11 535.2 0 11 23 32 535.2 0 32 24 17 535.2 0 17 25 13 535.2 0 13 26 12 535.2 0 12 27 12 535.2 0 12 28 10 535.2 0 10 29 29 535.2 0 29 30 20 535.2 0 20 31 25 535.2 0 25 32 18 535.2 0 18 33 18 535.2 0 18 34 21 535.2 0 21 35 27 535.2 0 27 36 33 535.2 0 33 37 20 535.2 0 20 38 18 535.2 0 18 39 16 535.2 0 16 40 12 535.2 0 12 41 16 535.2 0 16 42 12 535.2 0 12 43 35 535.2 0 35 44 45 535.2 0 45 45 176 535.2 0 176 46 237 535.2 0 237 47 210 535.2 0 210 48 22 535.2 0 22 49 17 535.2 0 17 50 12 535.2 0 12 51 13 535.2 0 13 52 8 535.2 0 8 53 16 535.2 0 16 54 19 535.2 0 19 55 17 535.2 0 17 56 22 535.2 0 22 57 12 535.2 0 12 58 17 535.2 0 17 59 12 535.2 0 12 60 11 535.2 0 11 61 10 535.2 0 10 62 14 535.2 0 14 63 11 535.2 0 11 64 13 535.2 0 13 65 20 535.2 0 20 66 16 535.2 0 16 67 23 535.2 0 23 68 60 535.2 0 60 69 10 535.2 0 10 70 17 535.2 0 17 71 27 535.2 0 27 72 65 535.2 0 65 73 86 535.2 0 86 74 19 535.2 0 19 75 18 535.2 0 18 76 14 535.2 0 14 77 27 535.2 0 27 78 35 535.2 0 35 79 24 535.2 0 24 80 30 535.2 0 30 81 28 535.2 0 28 82 24 535.2 0 24 83 16 535.2 0 16 84 37 535.2 0 37 85 21 535.2 0 21 86 18 535.2 0 18 87 17 535.2 0 17 88 23 535.2 0 23 89 25 535.2 0 25 90 30 535.2 0 30 91 20 535.2 0 20 92 35 535.2 0 35 93 39 535.2 0 39 94 49 535.2 0 49 95 446 535.2 0 446 96 257 535.2 0 257 97 263 535.2 0 263 98 23 535.2 0 23 99 17 535.2 0 17 100 17 535.2 0 17 101 31 535.2 0 31 102 19 535.2 0 19 103 15 535.2 0 15 104 22 535.2 0 22 105 19 535.2 0 19 106 27 535.2 0 27 107 18 535.2 0 18 108 88 535.2 0 88 109 12 535.2 0 12 110 14 535.2 0 14 111 23 535.2 0 23 112 23 535.2 0 23 113 130 535.2 0 130 114 219 535.2 0 219 115 157 535.2 0 157 116 193 535.2 0 193 117 30 535.2 0 30 118 15 535.2 0 15 119 14 535.2 0 14 120 37 535.2 0 37 121 12 535.2 0 12 122 31 535.2 0 31 123 35 535.2 0 35 124 50 535.2 0 50 125 104 535.2 0 104 126 23 535.2 0 23 127 23 535.2 0 23 128 49 535.2 0 49 129 31 535.2 0 31 130 21 535.2 0 21 131 28 535.2 0 28 132 32 535.2 0 32 133 41 535.2 0 41 134 67 535.2 0 67 135 131 535.2 0 131 136 92 535.2 0 92 137 314 535.2 0 314 138 285 535.2 0 285 139 762 535.2 0 762 140 83 535.2 0 83 141 23 535.2 0 23 142 40 535.2 0 40 143 43 535.2 0 43 144 39 535.2 0 39 145 30 535.2 0 30 146 19 535.2 0 19 147 19 535.2 0 19 148 22 535.2 0 22 149 24 535.2 0 24 150 19 535.2 0 19 151 24 535.2 0 24 152 22 535.2 0 22 153 16 535.2 0 16 154 16 535.2 0 16 155 17 535.2 0 17 156 25 535.2 0 25 157 21 535.2 0 21 158 30 535.2 0 30 159 16 535.2 0 16 160 14 535.2 0 14 161 18 535.2 0 18 162 30 535.2 0 30 163 34 535.2 0 34 164 60 535.2 0 60 165 20 535.2 0 20 166 18 535.2 0 18 167 74 535.2 0 74 168 76 535.2 0 76 169 151 535.2 0 151 170 38 535.2 0 38 171 13 535.2 0 13 172 22 535.2 0 22 173 23 535.2 0 23 174 27 535.2 0 27 175 23 535.2 0 23 176 23 535.2 0 23 177 20 535.2 0 20 178 20 535.2 0 20 179 34 535.2 0 34 180 32 535.2 0 32 181 44 535.2 0 44 182 19 535.2 0 19 183 35 535.2 0 35 184 118 535.2 0 118 185 93 535.2 0 93 186 106 535.2 0 106 187 46 535.2 0 46 188 29 535.2 0 29 189 25 535.2 0 25 190 38 535.2 0 38 191 26 535.2 0 26 192 21 535.2 0 21 193 21 535.2 0 21 194 23 535.2 0 23 195 21 535.2 0 21 196 13 535.2 0 13 197 23 535.2 0 23 198 34 535.2 0 34 199 43 535.2 0 43 200 16 535.2 0 16 201 30 535.2 0 30 202 29 535.2 0 29 203 22 535.2 0 22 204 72 535.2 0 72 205 89 535.2 0 89 206 123 535.2 0 123 207 40 535.2 0 40 208 30 535.2 0 30 209 28 535.2 0 28 210 39 535.2 0 39 211 45 535.2 0 45 212 41 535.2 0 41 213 26 535.2 0 26 214 34 535.2 0 34 215 46 535.2 0 46 216 50 535.2 0 50 217 32 535.2 0 32 218 41 535.2 0 41 219 39 535.2 0 39 220 44 535.2 0 44 221 41 535.2 0 41 222 50 535.2 0 50 223 82 535.2 0 82 224 155 535.2 0 155 225 1885 535.2 0 1885 226 1362 535.2 0 1362 227 1310 535.2 0 1310 228 267 535.2 0 267 229 19 535.2 0 19 230 31 535.2 0 31 231 27 535.2 0 27 232 28 535.2 0 28 233 23 535.2 0 23 234 25 535.2 0 25 235 39 535.2 0 39 236 49 535.2 0 49 237 33 535.2 0 33 238 16 535.2 0 16 239 22 535.2 0 22 240 23 535.2 0 23 241 26 535.2 0 26 242 20 535.2 0 20 243 24 535.2 0 24 244 34 535.2 0 34 245 22 535.2 0 22 246 33 535.2 0 33 247 21 535.2 0 21 248 22 535.2 0 22 249 32 535.2 0 32 250 30 535.2 0 30 251 22 535.2 0 22 252 23 535.2 0 23 253 26 535.2 0 26 254 34 535.2 0 34 255 23 535.2 0 23 256 29 535.2 0 29 257 33 535.2 0 33 258 29 535.2 0 29 259 28 535.2 0 28 260 26 535.2 0 26 261 17 535.2 0 17 262 27 535.2 0 27 263 18 535.2 0 18 264 28 535.2 0 28 265 32 535.2 0 32 266 25 535.2 0 25 267 20 535.2 0 20 268 21 535.2 0 21 269 42 535.2 0 42 270 24 535.2 0 24 271 17 535.2 0 17 272 26 535.2 0 26 273 20 535.2 0 20 274 25 535.2 0 25 275 23 535.2 0 23 276 20 535.2 0 20 277 22 535.2 0 22 278 38 535.2 0 38 279 30 535.2 0 30 280 43 535.2 0 43 281 28 535.2 0 28 282 22 535.2 0 22 283 24 535.2 0 24 284 23 535.2 0 23 285 32 535.2 0 32 286 24 535.2 0 24 287 16 535.2 0 16 288 25 535.2 0 25 289 22 535.2 0 22 290 25 535.2 0 25 291 30 535.2 0 30 292 20 535.2 0 20 293 28 535.2 0 28 294 31 535.2 0 31 295 29 535.2 0 29 296 29 535.2 0 29 297 33 535.2 0 33 298 34 535.2 0 34 299 36 535.2 0 36 300 17 535.2 0 17 301 78 535.2 0 78 Finished in 13.49 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 285,637 Reads with adapters: 143,255 (50.2%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 143,213 (50.1%) Total basepairs processed: 82,623,961 bp Total written (filtered): 38,895,894 bp (47.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 143255 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 3 0.0 3 0 0 0 2 1 18 29 0.0 3 0 0 25 3 1 19 1105 0.0 3 0 1093 11 1 20 140903 0.0 4 138690 2129 77 6 1 21 373 0.0 4 112 250 9 1 1 22 35 0.0 4 6 0 27 2 23 116 0.0 4 3 0 2 101 10 24 3 0.0 4 2 0 0 0 1 25 2 0.0 4 2 26 2 0.0 4 1 0 1 27 3 0.0 4 0 0 0 0 3 47 1 0.0 4 0 0 0 0 1 89 3 0.0 4 0 0 0 0 3 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 123 1 0.0 4 1 124 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 620 0.0 4 0 0 0 0 620 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 1 138 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 158 1 0.0 4 1 159 1 0.0 4 0 1 162 1 0.0 4 0 1 176 1 0.0 4 0 0 0 0 1 190 1 0.0 4 1 192 1 0.0 4 0 0 0 0 1 197 2 0.0 4 2 203 1 0.0 4 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 1 228 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 243 1 0.0 4 1 247 1 0.0 4 1 259 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 1 282 5 0.0 4 0 0 0 0 5 283 2 0.0 4 0 0 0 0 2 284 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 1 292 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.18 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 143,213 Reads with adapters: 33,920 (23.7%) Reads written (passing filters): 143,213 (100.0%) Total basepairs processed: 38,895,894 bp Total written (filtered): 38,567,508 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 33920 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 2.1% G: 96.2% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 36 2237.7 0 36 4 42 559.4 0 42 5 63 139.9 0 63 6 11 35.0 0 11 7 4 8.7 0 4 8 3 2.2 0 3 9 24395 0.5 0 703 23692 10 8094 0.1 1 296 7798 11 1129 0.0 1 8 1121 12 38 0.0 1 0 38 13 13 0.0 1 1 12 14 2 0.0 1 0 2 18 1 0.0 1 0 1 23 1 0.0 1 1 43 1 0.0 1 1 57 1 0.0 1 0 1 68 1 0.0 1 0 1 77 1 0.0 1 0 1 80 1 0.0 1 1 81 1 0.0 1 1 85 5 0.0 1 5 86 1 0.0 1 1 101 1 0.0 1 1 108 1 0.0 1 1 147 1 0.0 1 1 150 32 0.0 1 2 30 151 4 0.0 1 0 4 152 2 0.0 1 0 2 160 1 0.0 1 1 162 1 0.0 1 1 169 4 0.0 1 4 177 3 0.0 1 0 3 189 3 0.0 1 0 3 208 13 0.0 1 8 5 209 2 0.0 1 2 210 1 0.0 1 1 226 4 0.0 1 3 1 228 1 0.0 1 1 258 1 0.0 1 0 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.