This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.88 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 476,681 (21.7%) Reads that were too short: 11,442 (0.5%) Reads written (passing filters): 465,239 (21.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 91,560,894 bp (13.9%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 476681 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1234 535.2 0 1234 7 63664 535.2 0 63664 8 51314 535.2 0 51314 9 126 535.2 0 126 10 108 535.2 0 108 11 95 535.2 0 95 12 101 535.2 0 101 13 76 535.2 0 76 14 79 535.2 0 79 15 96 535.2 0 96 16 68 535.2 0 68 17 81 535.2 0 81 18 59 535.2 0 59 19 74 535.2 0 74 20 67 535.2 0 67 21 75 535.2 0 75 22 97 535.2 0 97 23 103 535.2 0 103 24 90 535.2 0 90 25 165 535.2 0 165 26 211 535.2 0 211 27 248 535.2 0 248 28 84 535.2 0 84 29 92 535.2 0 92 30 94 535.2 0 94 31 58 535.2 0 58 32 79 535.2 0 79 33 58 535.2 0 58 34 171 535.2 0 171 35 108 535.2 0 108 36 102 535.2 0 102 37 103 535.2 0 103 38 71 535.2 0 71 39 54 535.2 0 54 40 68 535.2 0 68 41 65 535.2 0 65 42 86 535.2 0 86 43 102 535.2 0 102 44 57 535.2 0 57 45 65 535.2 0 65 46 89 535.2 0 89 47 56 535.2 0 56 48 74 535.2 0 74 49 80 535.2 0 80 50 132 535.2 0 132 51 81 535.2 0 81 52 72 535.2 0 72 53 80 535.2 0 80 54 93 535.2 0 93 55 75 535.2 0 75 56 91 535.2 0 91 57 90 535.2 0 90 58 89 535.2 0 89 59 77 535.2 0 77 60 98 535.2 0 98 61 106 535.2 0 106 62 112 535.2 0 112 63 103 535.2 0 103 64 118 535.2 0 118 65 81 535.2 0 81 66 106 535.2 0 106 67 114 535.2 0 114 68 151 535.2 0 151 69 141 535.2 0 141 70 133 535.2 0 133 71 103 535.2 0 103 72 210 535.2 0 210 73 233 535.2 0 233 74 303 535.2 0 303 75 425 535.2 0 425 76 217 535.2 0 217 77 242 535.2 0 242 78 332 535.2 0 332 79 1659 535.2 0 1659 80 1066 535.2 0 1066 81 1761 535.2 0 1761 82 794 535.2 0 794 83 1120 535.2 0 1120 84 276 535.2 0 276 85 331 535.2 0 331 86 342 535.2 0 342 87 111 535.2 0 111 88 120 535.2 0 120 89 121 535.2 0 121 90 104 535.2 0 104 91 105 535.2 0 105 92 107 535.2 0 107 93 151 535.2 0 151 94 185 535.2 0 185 95 180 535.2 0 180 96 165 535.2 0 165 97 139 535.2 0 139 98 148 535.2 0 148 99 117 535.2 0 117 100 179 535.2 0 179 101 240 535.2 0 240 102 204 535.2 0 204 103 225 535.2 0 225 104 256 535.2 0 256 105 504 535.2 0 504 106 221 535.2 0 221 107 306 535.2 0 306 108 497 535.2 0 497 109 298 535.2 0 298 110 310 535.2 0 310 111 506 535.2 0 506 112 423 535.2 0 423 113 510 535.2 0 510 114 475 535.2 0 475 115 761 535.2 0 761 116 910 535.2 0 910 117 1365 535.2 0 1365 118 14471 535.2 0 14471 119 22168 535.2 0 22168 120 22042 535.2 0 22042 121 4780 535.2 0 4780 122 5522 535.2 0 5522 123 3605 535.2 0 3605 124 612 535.2 0 612 125 391 535.2 0 391 126 613 535.2 0 613 127 493 535.2 0 493 128 401 535.2 0 401 129 392 535.2 0 392 130 500 535.2 0 500 131 831 535.2 0 831 132 1343 535.2 0 1343 133 728 535.2 0 728 134 772 535.2 0 772 135 1192 535.2 0 1192 136 837 535.2 0 837 137 1219 535.2 0 1219 138 1714 535.2 0 1714 139 3903 535.2 0 3903 140 8644 535.2 0 8644 141 26671 535.2 0 26671 142 15177 535.2 0 15177 143 2498 535.2 0 2498 144 3184 535.2 0 3184 145 26957 535.2 0 26957 146 53621 535.2 0 53621 147 58999 535.2 0 58999 148 7942 535.2 0 7942 149 2826 535.2 0 2826 150 831 535.2 0 831 151 462 535.2 0 462 152 407 535.2 0 407 153 272 535.2 0 272 154 324 535.2 0 324 155 347 535.2 0 347 156 286 535.2 0 286 157 299 535.2 0 299 158 492 535.2 0 492 159 552 535.2 0 552 160 1601 535.2 0 1601 161 324 535.2 0 324 162 474 535.2 0 474 163 690 535.2 0 690 164 753 535.2 0 753 165 4273 535.2 0 4273 166 7136 535.2 0 7136 167 4231 535.2 0 4231 168 422 535.2 0 422 169 286 535.2 0 286 170 153 535.2 0 153 171 103 535.2 0 103 172 114 535.2 0 114 173 125 535.2 0 125 174 132 535.2 0 132 175 125 535.2 0 125 176 88 535.2 0 88 177 110 535.2 0 110 178 100 535.2 0 100 179 99 535.2 0 99 180 94 535.2 0 94 181 157 535.2 0 157 182 129 535.2 0 129 183 108 535.2 0 108 184 139 535.2 0 139 185 102 535.2 0 102 186 115 535.2 0 115 187 110 535.2 0 110 188 94 535.2 0 94 189 104 535.2 0 104 190 106 535.2 0 106 191 119 535.2 0 119 192 174 535.2 0 174 193 291 535.2 0 291 194 317 535.2 0 317 195 137 535.2 0 137 196 93 535.2 0 93 197 124 535.2 0 124 198 104 535.2 0 104 199 132 535.2 0 132 200 153 535.2 0 153 201 131 535.2 0 131 202 111 535.2 0 111 203 95 535.2 0 95 204 95 535.2 0 95 205 109 535.2 0 109 206 144 535.2 0 144 207 126 535.2 0 126 208 112 535.2 0 112 209 138 535.2 0 138 210 128 535.2 0 128 211 128 535.2 0 128 212 65 535.2 0 65 213 97 535.2 0 97 214 87 535.2 0 87 215 98 535.2 0 98 216 268 535.2 0 268 217 423 535.2 0 423 218 344 535.2 0 344 219 260 535.2 0 260 220 109 535.2 0 109 221 101 535.2 0 101 222 79 535.2 0 79 223 108 535.2 0 108 224 101 535.2 0 101 225 129 535.2 0 129 226 149 535.2 0 149 227 127 535.2 0 127 228 104 535.2 0 104 229 69 535.2 0 69 230 88 535.2 0 88 231 85 535.2 0 85 232 73 535.2 0 73 233 78 535.2 0 78 234 102 535.2 0 102 235 80 535.2 0 80 236 87 535.2 0 87 237 109 535.2 0 109 238 88 535.2 0 88 239 85 535.2 0 85 240 87 535.2 0 87 241 99 535.2 0 99 242 84 535.2 0 84 243 99 535.2 0 99 244 111 535.2 0 111 245 93 535.2 0 93 246 118 535.2 0 118 247 152 535.2 0 152 248 140 535.2 0 140 249 86 535.2 0 86 250 92 535.2 0 92 251 72 535.2 0 72 252 87 535.2 0 87 253 101 535.2 0 101 254 112 535.2 0 112 255 110 535.2 0 110 256 108 535.2 0 108 257 89 535.2 0 89 258 91 535.2 0 91 259 93 535.2 0 93 260 99 535.2 0 99 261 83 535.2 0 83 262 100 535.2 0 100 263 89 535.2 0 89 264 107 535.2 0 107 265 145 535.2 0 145 266 122 535.2 0 122 267 134 535.2 0 134 268 82 535.2 0 82 269 64 535.2 0 64 270 119 535.2 0 119 271 91 535.2 0 91 272 95 535.2 0 95 273 139 535.2 0 139 274 128 535.2 0 128 275 128 535.2 0 128 276 91 535.2 0 91 277 117 535.2 0 117 278 110 535.2 0 110 279 117 535.2 0 117 280 95 535.2 0 95 281 100 535.2 0 100 282 88 535.2 0 88 283 82 535.2 0 82 284 73 535.2 0 73 285 93 535.2 0 93 286 90 535.2 0 90 287 93 535.2 0 93 288 119 535.2 0 119 289 98 535.2 0 98 290 109 535.2 0 109 291 116 535.2 0 116 292 93 535.2 0 93 293 86 535.2 0 86 294 99 535.2 0 99 295 105 535.2 0 105 296 108 535.2 0 108 297 127 535.2 0 127 298 121 535.2 0 121 299 144 535.2 0 144 300 157 535.2 0 157 301 283 535.2 0 283 Finished in 16.69 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 465,239 Reads with adapters: 71,886 (15.5%) Reads that were too short: 1,358 (0.3%) Reads written (passing filters): 70,528 (15.2%) Total basepairs processed: 91,560,894 bp Total written (filtered): 18,359,705 bp (20.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 71886 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 50 0.0 3 0 0 27 23 16 454 0.0 3 0 376 42 36 17 60891 0.0 3 55217 4597 852 225 18 167 0.0 3 13 121 17 16 19 1243 0.0 3 911 85 124 123 20 78 0.0 3 2 3 0 73 21 3 0.0 3 0 0 1 2 22 2 0.0 3 0 0 0 2 23 62 0.0 3 0 0 1 61 24 1 0.0 3 1 25 7 0.0 3 1 0 0 6 26 4493 0.0 3 0 0 69 4424 27 26 0.0 3 0 0 1 25 28 17 0.0 3 0 0 1 16 29 612 0.0 3 0 1 21 590 30 7 0.0 3 0 0 1 6 31 1 0.0 3 0 0 0 1 32 17 0.0 3 0 0 2 15 33 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 4 0.0 3 0 1 0 3 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 6 0.0 3 0 0 0 6 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 1 1 45 9 0.0 3 0 0 0 9 46 1 0.0 3 0 0 0 1 47 10 0.0 3 0 0 0 10 48 21 0.0 3 0 0 0 21 49 16 0.0 3 0 0 0 16 50 210 0.0 3 0 0 8 202 51 9 0.0 3 0 0 1 8 52 101 0.0 3 0 0 1 100 53 166 0.0 3 0 0 1 165 54 12 0.0 3 0 0 0 12 55 2 0.0 3 0 0 1 1 56 8 0.0 3 0 0 1 7 57 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 6 0.0 3 0 0 0 6 62 3 0.0 3 0 0 0 3 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 526 0.0 3 0 0 11 515 69 5 0.0 3 0 0 0 5 70 17 0.0 3 0 0 0 17 71 5 0.0 3 0 0 0 5 72 147 0.0 3 0 0 2 145 73 154 0.0 3 0 0 1 153 74 774 0.0 3 0 0 3 771 75 3 0.0 3 0 0 0 3 76 30 0.0 3 0 0 3 27 77 186 0.0 3 0 1 4 181 78 6 0.0 3 0 0 1 5 79 18 0.0 3 0 0 0 18 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 4 0.0 3 0 0 0 4 86 51 0.0 3 0 0 0 51 87 9 0.0 3 0 0 0 9 88 4 0.0 3 0 0 0 4 89 6 0.0 3 0 1 0 5 90 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 7 0.0 3 0 0 0 7 96 4 0.0 3 0 0 0 4 97 8 0.0 3 0 0 0 8 98 5 0.0 3 0 0 0 5 99 14 0.0 3 0 0 0 14 100 3 0.0 3 0 0 0 3 101 11 0.0 3 0 0 1 10 102 1 0.0 3 0 0 0 1 103 7 0.0 3 0 0 0 7 106 3 0.0 3 0 0 1 2 107 4 0.0 3 0 0 0 4 108 4 0.0 3 0 0 0 4 110 20 0.0 3 0 0 0 20 111 12 0.0 3 0 0 1 11 112 6 0.0 3 0 0 0 6 113 6 0.0 3 0 0 0 6 114 3 0.0 3 0 0 0 3 116 2 0.0 3 0 0 0 2 117 3 0.0 3 0 0 0 3 118 3 0.0 3 0 0 0 3 120 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 124 11 0.0 3 0 0 1 10 125 19 0.0 3 0 0 2 17 126 17 0.0 3 0 0 1 16 127 44 0.0 3 0 0 2 42 128 52 0.0 3 0 0 2 50 129 16 0.0 3 0 0 0 16 130 5 0.0 3 0 0 0 5 131 4 0.0 3 0 0 1 3 132 4 0.0 3 0 0 1 3 133 11 0.0 3 0 1 0 10 134 10 0.0 3 0 0 0 10 135 7 0.0 3 0 0 0 7 136 9 0.0 3 0 0 0 9 137 9 0.0 3 0 0 0 9 138 156 0.0 3 0 0 3 153 139 18 0.0 3 0 0 0 18 140 35 0.0 3 0 0 2 33 141 84 0.0 3 0 0 5 79 142 12 0.0 3 0 0 1 11 143 3 0.0 3 0 0 0 3 144 3 0.0 3 0 0 0 3 145 5 0.0 3 0 0 0 5 146 63 0.0 3 0 0 1 62 147 51 0.0 3 0 0 4 47 148 17 0.0 3 0 0 1 16 149 5 0.0 3 0 0 0 5 150 7 0.0 3 0 0 1 6 151 10 0.0 3 0 0 0 10 152 8 0.0 3 0 0 0 8 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 14 0.0 3 0 0 0 14 156 79 0.0 3 0 0 1 78 157 3 0.0 3 0 0 0 3 158 8 0.0 3 0 0 0 8 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 7 0.0 3 0 0 0 7 162 4 0.0 3 0 0 0 4 163 6 0.0 3 0 0 0 6 164 5 0.0 3 0 0 0 5 165 13 0.0 3 0 0 0 13 166 36 0.0 3 0 0 2 34 167 5 0.0 3 0 0 1 4 169 4 0.0 3 0 0 0 4 170 4 0.0 3 0 0 0 4 171 4 0.0 3 0 0 2 2 172 11 0.0 3 0 0 1 10 173 4 0.0 3 0 0 0 4 174 5 0.0 3 0 0 0 5 175 2 0.0 3 0 0 0 2 176 8 0.0 3 0 0 1 7 177 1 0.0 3 0 0 0 1 178 3 0.0 3 0 0 0 3 179 4 0.0 3 0 0 0 4 180 8 0.0 3 0 0 0 8 181 3 0.0 3 0 0 0 3 182 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 187 3 0.0 3 0 0 0 3 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 194 8 0.0 3 0 0 0 8 196 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 199 3 0.0 3 0 0 0 3 200 2 0.0 3 0 0 0 2 205 3 0.0 3 0 0 0 3 208 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 0 2 210 3 0.0 3 0 0 0 3 211 3 0.0 3 0 0 1 2 212 8 0.0 3 0 0 1 7 215 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 219 1 0.0 3 0 1 221 3 0.0 3 0 0 0 3 223 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 3 0.0 3 0 0 0 3 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 1 268 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 273 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 3 0.0 3 0 0 1 2 281 10 0.0 3 0 0 0 10 282 8 0.0 3 0 0 0 8 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.37 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 70,528 Reads with adapters: 295 (0.4%) Reads written (passing filters): 70,528 (100.0%) Total basepairs processed: 18,359,705 bp Total written (filtered): 18,358,798 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 295 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.1% C: 40.7% G: 23.7% T: 9.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 281 1102.0 0 281 4 10 275.5 0 10 6 4 17.2 0 4