This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.92 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 246,440 (11.2%) Reads that were too short: 20,703 (0.9%) Reads written (passing filters): 225,737 (10.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 60,995,284 bp (9.2%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 246440 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 7205 535.2 0 7205 7 77156 535.2 0 77156 8 7738 535.2 0 7738 9 94653 535.2 0 94653 10 103 535.2 0 103 11 41 535.2 0 41 12 45 535.2 0 45 13 44 535.2 0 44 14 41 535.2 0 41 15 43 535.2 0 43 16 42 535.2 0 42 17 40 535.2 0 40 18 37 535.2 0 37 19 39 535.2 0 39 20 36 535.2 0 36 21 42 535.2 0 42 22 58 535.2 0 58 23 44 535.2 0 44 24 21 535.2 0 21 25 26 535.2 0 26 26 40 535.2 0 40 27 44 535.2 0 44 28 73 535.2 0 73 29 60 535.2 0 60 30 52 535.2 0 52 31 43 535.2 0 43 32 36 535.2 0 36 33 341 535.2 0 341 34 364 535.2 0 364 35 736 535.2 0 736 36 30 535.2 0 30 37 32 535.2 0 32 38 30 535.2 0 30 39 78 535.2 0 78 40 34 535.2 0 34 41 27 535.2 0 27 42 30 535.2 0 30 43 32 535.2 0 32 44 54 535.2 0 54 45 61 535.2 0 61 46 61 535.2 0 61 47 50 535.2 0 50 48 42 535.2 0 42 49 49 535.2 0 49 50 49 535.2 0 49 51 39 535.2 0 39 52 43 535.2 0 43 53 46 535.2 0 46 54 51 535.2 0 51 55 53 535.2 0 53 56 87 535.2 0 87 57 280 535.2 0 280 58 474 535.2 0 474 59 204 535.2 0 204 60 61 535.2 0 61 61 38 535.2 0 38 62 39 535.2 0 39 63 50 535.2 0 50 64 74 535.2 0 74 65 47 535.2 0 47 66 56 535.2 0 56 67 51 535.2 0 51 68 69 535.2 0 69 69 61 535.2 0 61 70 44 535.2 0 44 71 37 535.2 0 37 72 145 535.2 0 145 73 358 535.2 0 358 74 166 535.2 0 166 75 47 535.2 0 47 76 40 535.2 0 40 77 70 535.2 0 70 78 47 535.2 0 47 79 64 535.2 0 64 80 38 535.2 0 38 81 42 535.2 0 42 82 49 535.2 0 49 83 51 535.2 0 51 84 134 535.2 0 134 85 85 535.2 0 85 86 193 535.2 0 193 87 104 535.2 0 104 88 50 535.2 0 50 89 51 535.2 0 51 90 89 535.2 0 89 91 80 535.2 0 80 92 73 535.2 0 73 93 53 535.2 0 53 94 117 535.2 0 117 95 73 535.2 0 73 96 71 535.2 0 71 97 84 535.2 0 84 98 85 535.2 0 85 99 306 535.2 0 306 100 41 535.2 0 41 101 52 535.2 0 52 102 110 535.2 0 110 103 47 535.2 0 47 104 67 535.2 0 67 105 83 535.2 0 83 106 251 535.2 0 251 107 940 535.2 0 940 108 1962 535.2 0 1962 109 1180 535.2 0 1180 110 78 535.2 0 78 111 59 535.2 0 59 112 45 535.2 0 45 113 45 535.2 0 45 114 45 535.2 0 45 115 58 535.2 0 58 116 48 535.2 0 48 117 41 535.2 0 41 118 47 535.2 0 47 119 31 535.2 0 31 120 48 535.2 0 48 121 82 535.2 0 82 122 66 535.2 0 66 123 85 535.2 0 85 124 62 535.2 0 62 125 45 535.2 0 45 126 69 535.2 0 69 127 492 535.2 0 492 128 685 535.2 0 685 129 482 535.2 0 482 130 183 535.2 0 183 131 76 535.2 0 76 132 54 535.2 0 54 133 32 535.2 0 32 134 74 535.2 0 74 135 125 535.2 0 125 136 165 535.2 0 165 137 121 535.2 0 121 138 139 535.2 0 139 139 146 535.2 0 146 140 136 535.2 0 136 141 188 535.2 0 188 142 300 535.2 0 300 143 571 535.2 0 571 144 553 535.2 0 553 145 470 535.2 0 470 146 200 535.2 0 200 147 248 535.2 0 248 148 60 535.2 0 60 149 97 535.2 0 97 150 105 535.2 0 105 151 77 535.2 0 77 152 150 535.2 0 150 153 206 535.2 0 206 154 356 535.2 0 356 155 385 535.2 0 385 156 238 535.2 0 238 157 92 535.2 0 92 158 266 535.2 0 266 159 340 535.2 0 340 160 104 535.2 0 104 161 117 535.2 0 117 162 130 535.2 0 130 163 66 535.2 0 66 164 60 535.2 0 60 165 59 535.2 0 59 166 101 535.2 0 101 167 110 535.2 0 110 168 81 535.2 0 81 169 119 535.2 0 119 170 76 535.2 0 76 171 126 535.2 0 126 172 60 535.2 0 60 173 74 535.2 0 74 174 116 535.2 0 116 175 246 535.2 0 246 176 213 535.2 0 213 177 79 535.2 0 79 178 89 535.2 0 89 179 91 535.2 0 91 180 89 535.2 0 89 181 108 535.2 0 108 182 160 535.2 0 160 183 246 535.2 0 246 184 383 535.2 0 383 185 396 535.2 0 396 186 3556 535.2 0 3556 187 6439 535.2 0 6439 188 3178 535.2 0 3178 189 69 535.2 0 69 190 40 535.2 0 40 191 65 535.2 0 65 192 47 535.2 0 47 193 77 535.2 0 77 194 283 535.2 0 283 195 69 535.2 0 69 196 56 535.2 0 56 197 49 535.2 0 49 198 61 535.2 0 61 199 54 535.2 0 54 200 70 535.2 0 70 201 55 535.2 0 55 202 42 535.2 0 42 203 91 535.2 0 91 204 121 535.2 0 121 205 104 535.2 0 104 206 89 535.2 0 89 207 68 535.2 0 68 208 150 535.2 0 150 209 138 535.2 0 138 210 163 535.2 0 163 211 62 535.2 0 62 212 71 535.2 0 71 213 78 535.2 0 78 214 65 535.2 0 65 215 76 535.2 0 76 216 105 535.2 0 105 217 369 535.2 0 369 218 470 535.2 0 470 219 567 535.2 0 567 220 57 535.2 0 57 221 73 535.2 0 73 222 85 535.2 0 85 223 89 535.2 0 89 224 60 535.2 0 60 225 58 535.2 0 58 226 90 535.2 0 90 227 106 535.2 0 106 228 139 535.2 0 139 229 51 535.2 0 51 230 75 535.2 0 75 231 75 535.2 0 75 232 103 535.2 0 103 233 72 535.2 0 72 234 113 535.2 0 113 235 76 535.2 0 76 236 121 535.2 0 121 237 133 535.2 0 133 238 91 535.2 0 91 239 85 535.2 0 85 240 73 535.2 0 73 241 174 535.2 0 174 242 209 535.2 0 209 243 351 535.2 0 351 244 178 535.2 0 178 245 191 535.2 0 191 246 111 535.2 0 111 247 98 535.2 0 98 248 86 535.2 0 86 249 94 535.2 0 94 250 675 535.2 0 675 251 1081 535.2 0 1081 252 1104 535.2 0 1104 253 152 535.2 0 152 254 248 535.2 0 248 255 111 535.2 0 111 256 76 535.2 0 76 257 132 535.2 0 132 258 123 535.2 0 123 259 77 535.2 0 77 260 88 535.2 0 88 261 132 535.2 0 132 262 180 535.2 0 180 263 150 535.2 0 150 264 131 535.2 0 131 265 75 535.2 0 75 266 99 535.2 0 99 267 75 535.2 0 75 268 61 535.2 0 61 269 79 535.2 0 79 270 197 535.2 0 197 271 458 535.2 0 458 272 254 535.2 0 254 273 194 535.2 0 194 274 143 535.2 0 143 275 127 535.2 0 127 276 79 535.2 0 79 277 101 535.2 0 101 278 202 535.2 0 202 279 210 535.2 0 210 280 688 535.2 0 688 281 788 535.2 0 788 282 865 535.2 0 865 283 514 535.2 0 514 284 95 535.2 0 95 285 178 535.2 0 178 286 173 535.2 0 173 287 123 535.2 0 123 288 83 535.2 0 83 289 178 535.2 0 178 290 239 535.2 0 239 291 219 535.2 0 219 292 165 535.2 0 165 293 96 535.2 0 96 294 181 535.2 0 181 295 221 535.2 0 221 296 352 535.2 0 352 297 871 535.2 0 871 298 744 535.2 0 744 299 678 535.2 0 678 300 209 535.2 0 209 301 83 535.2 0 83 Finished in 9.76 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 225,737 Reads with adapters: 103,878 (46.0%) Reads that were too short: 209 (0.1%) Reads written (passing filters): 103,669 (45.9%) Total basepairs processed: 60,995,284 bp Total written (filtered): 28,471,301 bp (46.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 103878 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 37 0.0 2 0 0 0 37 15 90 0.0 3 0 0 51 39 16 985 0.0 3 0 785 111 89 17 101488 0.0 3 91812 7619 1631 426 18 241 0.0 3 37 161 29 14 19 408 0.0 3 2 2 235 169 20 94 0.0 3 3 3 3 85 21 2 0.0 3 0 1 0 1 23 3 0.0 3 1 0 0 2 25 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 5 0.0 3 0 0 0 5 31 26 0.0 3 0 0 0 26 33 2 0.0 3 0 0 0 2 34 4 0.0 3 0 0 0 4 37 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 4 0.0 3 1 0 0 3 46 1 0.0 3 0 0 0 1 48 28 0.0 3 0 0 0 28 50 1 0.0 3 0 0 0 1 51 7 0.0 3 0 0 1 6 52 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 49 0.0 3 0 0 0 49 60 17 0.0 3 0 0 0 17 61 9 0.0 3 0 0 2 7 62 11 0.0 3 0 0 0 11 63 2 0.0 3 0 0 1 1 64 12 0.0 3 0 0 0 12 65 1 0.0 3 0 0 1 66 7 0.0 3 0 0 0 7 67 6 0.0 3 0 0 0 6 68 4 0.0 3 0 0 1 3 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 1 72 3 0.0 3 0 0 0 3 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 1 0.0 3 1 80 3 0.0 3 0 0 0 3 81 3 0.0 3 0 0 0 3 82 2 0.0 3 0 0 0 2 83 4 0.0 3 0 0 0 4 84 8 0.0 3 0 0 0 8 85 22 0.0 3 0 0 0 22 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 1 2 110 7 0.0 3 0 0 1 6 111 4 0.0 3 0 0 1 3 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 132 70 0.0 3 0 0 0 70 134 9 0.0 3 0 0 0 9 135 3 0.0 3 0 0 0 3 137 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 139 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 1 143 5 0.0 3 0 0 0 5 146 2 0.0 3 0 0 0 2 147 4 0.0 3 0 0 0 4 149 1 0.0 3 0 0 0 1 150 4 0.0 3 0 0 0 4 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 1 2 158 4 0.0 3 0 0 0 4 160 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 165 6 0.0 3 0 0 0 6 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 1 170 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 0 3 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 4 0.0 3 0 0 0 4 178 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 0 3 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 2 190 1 0.0 3 0 0 0 1 193 3 0.0 3 0 0 0 3 194 4 0.0 3 0 1 1 2 199 1 0.0 3 0 0 0 1 207 2 0.0 3 1 0 0 1 210 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 1 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 1 221 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 230 2 0.0 3 1 0 0 1 232 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 255 2 0.0 3 0 0 0 2 257 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 0 3 281 6 0.0 3 0 0 0 6 282 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 3 0.0 3 0 0 0 3 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 3 0.0 3 0 0 0 3 291 3 0.0 3 0 0 0 3 293 1 0.0 3 0 0 0 1 294 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.60 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 103,669 Reads with adapters: 492 (0.5%) Reads written (passing filters): 103,669 (100.0%) Total basepairs processed: 28,471,301 bp Total written (filtered): 28,469,722 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 492 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.4% C: 13.0% G: 45.3% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 412 1619.8 0 412 4 75 405.0 0 75 5 1 101.2 0 1 6 1 25.3 0 1 9 1 0.4 0 0 1 11 1 0.0 1 0 1 12 1 0.0 1 0 1