This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.41 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 81,590 (3.7%) Reads that were too short: 10,449 (0.5%) Reads written (passing filters): 71,141 (3.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 18,361,463 bp (2.8%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 81590 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 337 535.2 0 337 7 1439 535.2 0 1439 8 25893 535.2 0 25893 9 22309 535.2 0 22309 10 79 535.2 0 79 11 49 535.2 0 49 12 19 535.2 0 19 13 24 535.2 0 24 14 28 535.2 0 28 15 19 535.2 0 19 16 19 535.2 0 19 17 26 535.2 0 26 18 8 535.2 0 8 19 24 535.2 0 24 20 10 535.2 0 10 21 13 535.2 0 13 22 15 535.2 0 15 23 11 535.2 0 11 24 15 535.2 0 15 25 17 535.2 0 17 26 14 535.2 0 14 27 8 535.2 0 8 28 11 535.2 0 11 29 13 535.2 0 13 30 14 535.2 0 14 31 14 535.2 0 14 32 15 535.2 0 15 33 10 535.2 0 10 34 14 535.2 0 14 35 16 535.2 0 16 36 23 535.2 0 23 37 14 535.2 0 14 38 7 535.2 0 7 39 15 535.2 0 15 40 34 535.2 0 34 41 17 535.2 0 17 42 36 535.2 0 36 43 16 535.2 0 16 44 22 535.2 0 22 45 31 535.2 0 31 46 23 535.2 0 23 47 63 535.2 0 63 48 33 535.2 0 33 49 102 535.2 0 102 50 367 535.2 0 367 51 23 535.2 0 23 52 26 535.2 0 26 53 41 535.2 0 41 54 17 535.2 0 17 55 35 535.2 0 35 56 11 535.2 0 11 57 19 535.2 0 19 58 13 535.2 0 13 59 27 535.2 0 27 60 48 535.2 0 48 61 21 535.2 0 21 62 25 535.2 0 25 63 18 535.2 0 18 64 16 535.2 0 16 65 20 535.2 0 20 66 152 535.2 0 152 67 158 535.2 0 158 68 211 535.2 0 211 69 17 535.2 0 17 70 34 535.2 0 34 71 31 535.2 0 31 72 25 535.2 0 25 73 19 535.2 0 19 74 19 535.2 0 19 75 21 535.2 0 21 76 23 535.2 0 23 77 15 535.2 0 15 78 12 535.2 0 12 79 14 535.2 0 14 80 26 535.2 0 26 81 29 535.2 0 29 82 28 535.2 0 28 83 58 535.2 0 58 84 66 535.2 0 66 85 88 535.2 0 88 86 38 535.2 0 38 87 30 535.2 0 30 88 24 535.2 0 24 89 33 535.2 0 33 90 39 535.2 0 39 91 62 535.2 0 62 92 55 535.2 0 55 93 74 535.2 0 74 94 43 535.2 0 43 95 29 535.2 0 29 96 31 535.2 0 31 97 51 535.2 0 51 98 47 535.2 0 47 99 28 535.2 0 28 100 26 535.2 0 26 101 30 535.2 0 30 102 33 535.2 0 33 103 43 535.2 0 43 104 65 535.2 0 65 105 46 535.2 0 46 106 39 535.2 0 39 107 47 535.2 0 47 108 54 535.2 0 54 109 41 535.2 0 41 110 85 535.2 0 85 111 458 535.2 0 458 112 645 535.2 0 645 113 577 535.2 0 577 114 91 535.2 0 91 115 579 535.2 0 579 116 623 535.2 0 623 117 1840 535.2 0 1840 118 1715 535.2 0 1715 119 1949 535.2 0 1949 120 46 535.2 0 46 121 50 535.2 0 50 122 32 535.2 0 32 123 20 535.2 0 20 124 28 535.2 0 28 125 46 535.2 0 46 126 33 535.2 0 33 127 96 535.2 0 96 128 186 535.2 0 186 129 48 535.2 0 48 130 41 535.2 0 41 131 84 535.2 0 84 132 91 535.2 0 91 133 101 535.2 0 101 134 48 535.2 0 48 135 28 535.2 0 28 136 39 535.2 0 39 137 51 535.2 0 51 138 95 535.2 0 95 139 51 535.2 0 51 140 46 535.2 0 46 141 38 535.2 0 38 142 62 535.2 0 62 143 44 535.2 0 44 144 58 535.2 0 58 145 75 535.2 0 75 146 192 535.2 0 192 147 94 535.2 0 94 148 83 535.2 0 83 149 78 535.2 0 78 150 116 535.2 0 116 151 828 535.2 0 828 152 1510 535.2 0 1510 153 1378 535.2 0 1378 154 250 535.2 0 250 155 39 535.2 0 39 156 37 535.2 0 37 157 42 535.2 0 42 158 28 535.2 0 28 159 46 535.2 0 46 160 41 535.2 0 41 161 31 535.2 0 31 162 90 535.2 0 90 163 91 535.2 0 91 164 126 535.2 0 126 165 46 535.2 0 46 166 45 535.2 0 45 167 29 535.2 0 29 168 27 535.2 0 27 169 48 535.2 0 48 170 31 535.2 0 31 171 27 535.2 0 27 172 33 535.2 0 33 173 56 535.2 0 56 174 34 535.2 0 34 175 39 535.2 0 39 176 37 535.2 0 37 177 37 535.2 0 37 178 39 535.2 0 39 179 152 535.2 0 152 180 234 535.2 0 234 181 162 535.2 0 162 182 193 535.2 0 193 183 43 535.2 0 43 184 176 535.2 0 176 185 75 535.2 0 75 186 28 535.2 0 28 187 43 535.2 0 43 188 35 535.2 0 35 189 87 535.2 0 87 190 43 535.2 0 43 191 54 535.2 0 54 192 72 535.2 0 72 193 49 535.2 0 49 194 37 535.2 0 37 195 35 535.2 0 35 196 30 535.2 0 30 197 41 535.2 0 41 198 32 535.2 0 32 199 31 535.2 0 31 200 32 535.2 0 32 201 30 535.2 0 30 202 25 535.2 0 25 203 48 535.2 0 48 204 48 535.2 0 48 205 50 535.2 0 50 206 48 535.2 0 48 207 41 535.2 0 41 208 45 535.2 0 45 209 39 535.2 0 39 210 47 535.2 0 47 211 55 535.2 0 55 212 80 535.2 0 80 213 82 535.2 0 82 214 84 535.2 0 84 215 50 535.2 0 50 216 65 535.2 0 65 217 127 535.2 0 127 218 437 535.2 0 437 219 288 535.2 0 288 220 193 535.2 0 193 221 107 535.2 0 107 222 30 535.2 0 30 223 45 535.2 0 45 224 64 535.2 0 64 225 90 535.2 0 90 226 72 535.2 0 72 227 87 535.2 0 87 228 89 535.2 0 89 229 108 535.2 0 108 230 123 535.2 0 123 231 250 535.2 0 250 232 277 535.2 0 277 233 423 535.2 0 423 234 202 535.2 0 202 235 148 535.2 0 148 236 123 535.2 0 123 237 92 535.2 0 92 238 125 535.2 0 125 239 109 535.2 0 109 240 54 535.2 0 54 241 33 535.2 0 33 242 68 535.2 0 68 243 90 535.2 0 90 244 83 535.2 0 83 245 143 535.2 0 143 246 211 535.2 0 211 247 269 535.2 0 269 248 135 535.2 0 135 249 91 535.2 0 91 250 81 535.2 0 81 251 108 535.2 0 108 252 168 535.2 0 168 253 130 535.2 0 130 254 97 535.2 0 97 255 80 535.2 0 80 256 103 535.2 0 103 257 66 535.2 0 66 258 58 535.2 0 58 259 91 535.2 0 91 260 49 535.2 0 49 261 37 535.2 0 37 262 38 535.2 0 38 263 36 535.2 0 36 264 51 535.2 0 51 265 73 535.2 0 73 266 95 535.2 0 95 267 91 535.2 0 91 268 66 535.2 0 66 269 38 535.2 0 38 270 58 535.2 0 58 271 94 535.2 0 94 272 129 535.2 0 129 273 133 535.2 0 133 274 52 535.2 0 52 275 65 535.2 0 65 276 50 535.2 0 50 277 61 535.2 0 61 278 56 535.2 0 56 279 82 535.2 0 82 280 86 535.2 0 86 281 57 535.2 0 57 282 135 535.2 0 135 283 231 535.2 0 231 284 211 535.2 0 211 285 93 535.2 0 93 286 103 535.2 0 103 287 82 535.2 0 82 288 83 535.2 0 83 289 76 535.2 0 76 290 56 535.2 0 56 291 113 535.2 0 113 292 124 535.2 0 124 293 223 535.2 0 223 294 137 535.2 0 137 295 102 535.2 0 102 296 103 535.2 0 103 297 65 535.2 0 65 298 72 535.2 0 72 299 120 535.2 0 120 300 117 535.2 0 117 301 131 535.2 0 131 Finished in 3.39 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 71,141 Reads with adapters: 26,637 (37.4%) Reads that were too short: 164 (0.2%) Reads written (passing filters): 26,473 (37.2%) Total basepairs processed: 18,361,463 bp Total written (filtered): 7,263,961 bp (39.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26637 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 33 0.0 3 0 0 17 16 16 293 0.0 3 0 253 22 18 17 25589 0.0 3 23182 1906 383 118 18 77 0.0 3 2 61 9 5 19 126 0.0 3 2 0 68 56 20 58 0.0 3 1 1 0 56 23 1 0.0 3 0 0 0 1 24 3 0.0 3 0 0 0 3 27 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 30 3 0.0 3 0 0 0 3 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 54 0.0 3 0 0 1 53 43 10 0.0 3 0 0 0 10 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 54 83 0.0 3 0 0 0 83 55 6 0.0 3 0 0 0 6 56 9 0.0 3 0 0 1 8 57 11 0.0 3 0 0 0 11 58 26 0.0 3 0 0 0 26 59 1 0.0 3 0 0 0 1 61 4 0.0 3 0 0 0 4 62 3 0.0 3 0 0 0 3 63 7 0.0 3 0 0 0 7 65 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 91 5 0.0 3 0 0 1 4 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 1 1 104 1 0.0 3 0 0 0 1 105 14 0.0 3 0 0 0 14 106 4 0.0 3 0 0 0 4 107 3 0.0 3 0 0 1 2 108 3 0.0 3 0 0 0 3 109 4 0.0 3 0 0 1 3 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 2 0.0 3 0 0 1 1 141 6 0.0 3 0 0 0 6 142 8 0.0 3 0 0 0 8 143 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 1 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 3 0.0 3 0 0 0 3 161 2 0.0 3 0 0 1 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 166 13 0.0 3 0 0 1 12 167 5 0.0 3 0 0 0 5 168 2 0.0 3 0 0 0 2 169 11 0.0 3 0 0 0 11 170 4 0.0 3 0 0 0 4 171 2 0.0 3 0 0 0 2 172 3 0.0 3 0 0 0 3 173 7 0.0 3 0 0 0 7 174 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 3 0.0 3 0 0 1 2 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 1 1 198 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 281 8 0.0 3 0 0 0 8 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.92 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 26,473 Reads with adapters: 61 (0.2%) Reads written (passing filters): 26,473 (100.0%) Total basepairs processed: 7,263,961 bp Total written (filtered): 7,263,746 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 61 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.7% C: 27.9% G: 11.5% T: 23.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 46 413.6 0 46 4 8 103.4 0 8 5 5 25.9 0 5 10 2 0.0 1 0 2