This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.66 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 405,436 (18.5%) Reads that were too short: 47,779 (2.2%) Reads written (passing filters): 357,657 (16.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 89,729,926 bp (13.6%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 405436 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 308 535.2 0 308 7 4128 535.2 0 4128 8 3409 535.2 0 3409 9 212 535.2 0 212 10 275 535.2 0 275 11 172 535.2 0 172 12 205 535.2 0 205 13 321 535.2 0 321 14 415 535.2 0 415 15 402 535.2 0 402 16 372 535.2 0 372 17 527 535.2 0 527 18 676 535.2 0 676 19 1072 535.2 0 1072 20 16104 535.2 0 16104 21 25812 535.2 0 25812 22 28950 535.2 0 28950 23 345 535.2 0 345 24 265 535.2 0 265 25 408 535.2 0 408 26 171 535.2 0 171 27 144 535.2 0 144 28 118 535.2 0 118 29 176 535.2 0 176 30 113 535.2 0 113 31 210 535.2 0 210 32 306 535.2 0 306 33 350 535.2 0 350 34 312 535.2 0 312 35 652 535.2 0 652 36 1503 535.2 0 1503 37 426 535.2 0 426 38 482 535.2 0 482 39 1054 535.2 0 1054 40 545 535.2 0 545 41 623 535.2 0 623 42 1023 535.2 0 1023 43 1083 535.2 0 1083 44 1168 535.2 0 1168 45 1110 535.2 0 1110 46 1560 535.2 0 1560 47 1952 535.2 0 1952 48 3414 535.2 0 3414 49 54243 535.2 0 54243 50 88409 535.2 0 88409 51 84964 535.2 0 84964 52 369 535.2 0 369 53 68 535.2 0 68 54 62 535.2 0 62 55 45 535.2 0 45 56 68 535.2 0 68 57 83 535.2 0 83 58 78 535.2 0 78 59 52 535.2 0 52 60 78 535.2 0 78 61 104 535.2 0 104 62 156 535.2 0 156 63 743 535.2 0 743 64 974 535.2 0 974 65 1223 535.2 0 1223 66 38 535.2 0 38 67 49 535.2 0 49 68 53 535.2 0 53 69 31 535.2 0 31 70 58 535.2 0 58 71 57 535.2 0 57 72 61 535.2 0 61 73 66 535.2 0 66 74 84 535.2 0 84 75 63 535.2 0 63 76 59 535.2 0 59 77 44 535.2 0 44 78 35 535.2 0 35 79 52 535.2 0 52 80 38 535.2 0 38 81 54 535.2 0 54 82 49 535.2 0 49 83 67 535.2 0 67 84 62 535.2 0 62 85 41 535.2 0 41 86 63 535.2 0 63 87 63 535.2 0 63 88 89 535.2 0 89 89 72 535.2 0 72 90 91 535.2 0 91 91 45 535.2 0 45 92 60 535.2 0 60 93 41 535.2 0 41 94 59 535.2 0 59 95 47 535.2 0 47 96 40 535.2 0 40 97 61 535.2 0 61 98 59 535.2 0 59 99 35 535.2 0 35 100 32 535.2 0 32 101 50 535.2 0 50 102 38 535.2 0 38 103 33 535.2 0 33 104 34 535.2 0 34 105 55 535.2 0 55 106 74 535.2 0 74 107 56 535.2 0 56 108 37 535.2 0 37 109 45 535.2 0 45 110 45 535.2 0 45 111 47 535.2 0 47 112 50 535.2 0 50 113 49 535.2 0 49 114 45 535.2 0 45 115 67 535.2 0 67 116 64 535.2 0 64 117 324 535.2 0 324 118 426 535.2 0 426 119 632 535.2 0 632 120 133 535.2 0 133 121 85 535.2 0 85 122 45 535.2 0 45 123 68 535.2 0 68 124 103 535.2 0 103 125 103 535.2 0 103 126 157 535.2 0 157 127 97 535.2 0 97 128 79 535.2 0 79 129 48 535.2 0 48 130 51 535.2 0 51 131 74 535.2 0 74 132 56 535.2 0 56 133 66 535.2 0 66 134 76 535.2 0 76 135 61 535.2 0 61 136 43 535.2 0 43 137 40 535.2 0 40 138 75 535.2 0 75 139 82 535.2 0 82 140 101 535.2 0 101 141 79 535.2 0 79 142 88 535.2 0 88 143 73 535.2 0 73 144 127 535.2 0 127 145 98 535.2 0 98 146 102 535.2 0 102 147 70 535.2 0 70 148 69 535.2 0 69 149 76 535.2 0 76 150 59 535.2 0 59 151 64 535.2 0 64 152 99 535.2 0 99 153 119 535.2 0 119 154 105 535.2 0 105 155 75 535.2 0 75 156 55 535.2 0 55 157 90 535.2 0 90 158 145 535.2 0 145 159 174 535.2 0 174 160 71 535.2 0 71 161 56 535.2 0 56 162 54 535.2 0 54 163 50 535.2 0 50 164 62 535.2 0 62 165 82 535.2 0 82 166 51 535.2 0 51 167 71 535.2 0 71 168 76 535.2 0 76 169 62 535.2 0 62 170 65 535.2 0 65 171 62 535.2 0 62 172 98 535.2 0 98 173 91 535.2 0 91 174 79 535.2 0 79 175 75 535.2 0 75 176 104 535.2 0 104 177 152 535.2 0 152 178 244 535.2 0 244 179 210 535.2 0 210 180 201 535.2 0 201 181 166 535.2 0 166 182 1616 535.2 0 1616 183 929 535.2 0 929 184 1106 535.2 0 1106 185 97 535.2 0 97 186 135 535.2 0 135 187 89 535.2 0 89 188 140 535.2 0 140 189 196 535.2 0 196 190 244 535.2 0 244 191 187 535.2 0 187 192 178 535.2 0 178 193 1333 535.2 0 1333 194 1478 535.2 0 1478 195 1844 535.2 0 1844 196 1094 535.2 0 1094 197 1908 535.2 0 1908 198 475 535.2 0 475 199 91 535.2 0 91 200 126 535.2 0 126 201 118 535.2 0 118 202 84 535.2 0 84 203 97 535.2 0 97 204 169 535.2 0 169 205 98 535.2 0 98 206 140 535.2 0 140 207 166 535.2 0 166 208 133 535.2 0 133 209 93 535.2 0 93 210 87 535.2 0 87 211 100 535.2 0 100 212 104 535.2 0 104 213 467 535.2 0 467 214 1059 535.2 0 1059 215 1463 535.2 0 1463 216 1120 535.2 0 1120 217 273 535.2 0 273 218 329 535.2 0 329 219 608 535.2 0 608 220 723 535.2 0 723 221 789 535.2 0 789 222 372 535.2 0 372 223 251 535.2 0 251 224 101 535.2 0 101 225 100 535.2 0 100 226 140 535.2 0 140 227 153 535.2 0 153 228 92 535.2 0 92 229 128 535.2 0 128 230 90 535.2 0 90 231 142 535.2 0 142 232 195 535.2 0 195 233 201 535.2 0 201 234 167 535.2 0 167 235 144 535.2 0 144 236 420 535.2 0 420 237 273 535.2 0 273 238 518 535.2 0 518 239 929 535.2 0 929 240 2183 535.2 0 2183 241 1423 535.2 0 1423 242 405 535.2 0 405 243 58 535.2 0 58 244 77 535.2 0 77 245 118 535.2 0 118 246 166 535.2 0 166 247 188 535.2 0 188 248 153 535.2 0 153 249 190 535.2 0 190 250 559 535.2 0 559 251 1239 535.2 0 1239 252 1339 535.2 0 1339 253 531 535.2 0 531 254 106 535.2 0 106 255 127 535.2 0 127 256 163 535.2 0 163 257 216 535.2 0 216 258 234 535.2 0 234 259 1058 535.2 0 1058 260 2144 535.2 0 2144 261 1665 535.2 0 1665 262 225 535.2 0 225 263 856 535.2 0 856 264 1488 535.2 0 1488 265 1874 535.2 0 1874 266 456 535.2 0 456 267 562 535.2 0 562 268 279 535.2 0 279 269 266 535.2 0 266 270 295 535.2 0 295 271 195 535.2 0 195 272 147 535.2 0 147 273 88 535.2 0 88 274 120 535.2 0 120 275 169 535.2 0 169 276 169 535.2 0 169 277 149 535.2 0 149 278 151 535.2 0 151 279 93 535.2 0 93 280 106 535.2 0 106 281 172 535.2 0 172 282 163 535.2 0 163 283 270 535.2 0 270 284 291 535.2 0 291 285 294 535.2 0 294 286 244 535.2 0 244 287 243 535.2 0 243 288 2207 535.2 0 2207 289 3299 535.2 0 3299 290 3021 535.2 0 3021 291 418 535.2 0 418 292 166 535.2 0 166 293 322 535.2 0 322 294 255 535.2 0 255 295 275 535.2 0 275 296 154 535.2 0 154 297 163 535.2 0 163 298 261 535.2 0 261 299 325 535.2 0 325 300 460 535.2 0 460 301 228 535.2 0 228 Finished in 14.67 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 357,657 Reads with adapters: 14,959 (4.2%) Reads that were too short: 2,024 (0.6%) Reads written (passing filters): 12,935 (3.6%) Total basepairs processed: 89,729,926 bp Total written (filtered): 2,213,545 bp (2.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14959 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 0 3 16 40 0.0 3 0 34 2 4 17 3670 0.0 3 3343 264 51 12 18 18 0.0 3 1 10 4 3 19 10 0.0 3 0 0 2 8 20 15 0.0 3 2 1 0 12 22 1 0.0 3 0 0 1 23 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 7 0.0 3 0 0 0 7 29 4 0.0 3 0 0 0 4 30 1 0.0 3 0 0 0 1 31 6 0.0 3 0 0 0 6 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 19 0.0 3 0 0 0 19 35 7 0.0 3 0 0 0 7 36 4 0.0 3 0 0 0 4 37 2 0.0 3 0 0 0 2 38 5 0.0 3 0 0 0 5 40 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 46 17 0.0 3 0 0 0 17 47 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 4 0.0 3 0 0 1 3 51 72 0.0 3 0 0 0 72 52 4 0.0 3 0 0 0 4 53 6 0.0 3 0 0 0 6 54 2 0.0 3 1 0 0 1 55 3 0.0 3 0 0 0 3 56 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 3 0.0 3 0 0 0 3 63 3 0.0 3 0 0 0 3 64 3 0.0 3 0 0 0 3 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 1 1 68 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 12 0.0 3 0 0 0 12 72 10 0.0 3 0 0 0 10 73 561 0.0 3 0 0 4 557 74 45 0.0 3 0 0 1 44 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 16 0.0 3 0 0 1 15 81 1 0.0 3 0 0 0 1 82 3 0.0 3 0 0 0 3 83 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 5 0.0 3 0 0 0 5 89 6 0.0 3 1 0 0 5 90 6 0.0 3 0 0 0 6 91 6 0.0 3 0 0 0 6 92 19 0.0 3 0 0 0 19 93 8 0.0 3 0 0 0 8 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 97 57 0.0 3 0 0 0 57 98 7 0.0 3 0 0 0 7 99 8 0.0 3 0 0 0 8 100 8 0.0 3 0 0 0 8 101 16 0.0 3 0 0 0 16 102 62 0.0 3 0 0 0 62 103 7 0.0 3 0 0 1 6 104 12 0.0 3 0 0 0 12 106 2 0.0 3 0 0 0 2 108 8 0.0 3 0 0 0 8 109 3 0.0 3 0 0 0 3 111 4 0.0 3 0 0 0 4 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 117 75 0.0 3 0 0 0 75 118 35 0.0 3 0 0 0 35 119 7 0.0 3 0 0 0 7 120 21 0.0 3 0 0 0 21 121 25 0.0 3 0 0 0 25 122 3132 0.0 3 0 0 53 3079 123 445 0.0 3 0 0 2 443 124 110 0.0 3 0 0 1 109 125 477 0.0 3 0 0 18 459 126 93 0.0 3 0 0 1 92 127 26 0.0 3 0 0 1 25 128 91 0.0 3 0 0 2 89 129 12 0.0 3 0 0 1 11 130 22 0.0 3 0 0 0 22 131 31 0.0 3 0 0 2 29 132 6 0.0 3 0 0 0 6 133 19 0.0 3 0 0 0 19 134 26 0.0 3 0 0 0 26 135 14 0.0 3 0 0 2 12 136 20 0.0 3 0 0 2 18 137 63 0.0 3 0 0 0 63 138 60 0.0 3 0 0 2 58 139 23 0.0 3 0 0 3 20 140 16 0.0 3 0 0 0 16 141 31 0.0 3 0 0 5 26 142 1440 0.0 3 0 0 15 1425 143 44 0.0 3 0 0 2 42 144 14 0.0 3 0 0 0 14 145 206 0.0 3 0 0 5 201 146 85 0.0 3 0 0 2 83 147 14 0.0 3 0 0 0 14 148 97 0.0 3 0 0 3 94 149 39 0.0 3 0 0 1 38 150 12 0.0 3 0 0 0 12 151 436 0.0 3 0 0 5 431 152 46 0.0 3 0 0 0 46 153 27 0.0 3 0 0 0 27 154 77 0.0 3 0 0 1 76 155 15 0.0 3 0 0 0 15 156 8 0.0 3 0 0 0 8 157 3 0.0 3 0 0 0 3 158 7 0.0 3 0 1 0 6 159 4 0.0 3 0 0 0 4 160 4 0.0 3 1 0 0 3 161 5 0.0 3 0 0 0 5 162 3 0.0 3 0 0 1 2 163 4 0.0 3 0 0 0 4 164 17 0.0 3 0 0 0 17 165 8 0.0 3 0 0 0 8 166 24 0.0 3 0 0 0 24 167 9 0.0 3 0 0 1 8 168 62 0.0 3 0 0 3 59 169 7 0.0 3 0 0 0 7 170 25 0.0 3 0 0 0 25 171 409 0.0 3 0 0 7 402 172 168 0.0 3 0 0 2 166 173 58 0.0 3 0 0 1 57 174 66 0.0 3 0 0 6 60 175 46 0.0 3 0 1 3 42 176 5 0.0 3 0 0 0 5 177 40 0.0 3 0 0 1 39 178 10 0.0 3 0 0 0 10 179 9 0.0 3 0 0 0 9 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 1 2 183 8 0.0 3 0 0 0 8 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 4 0.0 3 0 0 0 4 188 8 0.0 3 0 0 0 8 189 9 0.0 3 0 0 0 9 190 2 0.0 3 0 0 0 2 191 4 0.0 3 0 0 0 4 192 7 0.0 3 0 0 0 7 193 53 0.0 3 0 0 2 51 194 8 0.0 3 0 0 1 7 195 18 0.0 3 0 0 0 18 196 3 0.0 3 0 0 0 3 197 15 0.0 3 0 0 0 15 198 2 0.0 3 0 0 0 2 199 2 0.0 3 0 0 0 2 201 2 0.0 3 0 0 0 2 202 51 0.0 3 0 0 0 51 203 15 0.0 3 0 0 1 14 204 7 0.0 3 0 0 0 7 205 10 0.0 3 0 0 0 10 206 4 0.0 3 0 0 0 4 207 3 0.0 3 0 0 1 2 208 3 0.0 3 0 0 0 3 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 1 1 211 3 0.0 3 0 0 0 3 212 2 0.0 3 0 0 0 2 213 5 0.0 3 0 0 0 5 214 3 0.0 3 0 0 0 3 215 8 0.0 3 0 0 1 7 216 3 0.0 3 0 0 0 3 217 1 0.0 3 0 0 0 1 218 9 0.0 3 0 0 1 8 219 10 0.0 3 0 0 0 10 220 2 0.0 3 0 0 0 2 221 6 0.0 3 0 0 0 6 222 29 0.0 3 0 0 0 29 223 10 0.0 3 0 0 0 10 224 5 0.0 3 0 0 0 5 225 4 0.0 3 0 0 0 4 226 3 0.0 3 0 0 0 3 227 2 0.0 3 0 0 0 2 228 4 0.0 3 0 0 0 4 229 9 0.0 3 0 1 0 8 230 8 0.0 3 0 0 1 7 231 11 0.0 3 0 0 0 11 232 9 0.0 3 0 0 0 9 233 6 0.0 3 0 0 0 6 234 144 0.0 3 0 0 4 140 235 85 0.0 3 0 0 5 80 236 50 0.0 3 0 0 1 49 237 59 0.0 3 0 0 1 58 238 89 0.0 3 0 0 2 87 239 31 0.0 3 0 0 0 31 240 9 0.0 3 0 0 0 9 241 11 0.0 3 0 0 0 11 242 53 0.0 3 0 0 2 51 243 101 0.0 3 0 0 6 95 244 193 0.0 3 0 0 14 179 245 16 0.0 3 0 0 1 15 246 4 0.0 3 0 0 0 4 247 9 0.0 3 0 0 0 9 248 7 0.0 3 0 0 0 7 249 8 0.0 3 0 0 0 8 250 49 0.0 3 0 0 1 48 251 17 0.0 3 0 0 0 17 252 75 0.0 3 0 0 0 75 253 5 0.0 3 0 0 0 5 254 4 0.0 3 0 0 1 3 255 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 258 3 0.0 3 0 0 0 3 259 2 0.0 3 0 0 0 2 260 4 0.0 3 0 0 0 4 261 9 0.0 3 0 0 0 9 262 25 0.0 3 0 0 2 23 263 13 0.0 3 0 0 0 13 264 7 0.0 3 0 0 0 7 265 6 0.0 3 0 0 0 6 266 12 0.0 3 0 0 2 10 267 15 0.0 3 0 0 2 13 268 2 0.0 3 0 0 1 1 269 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 17 0.0 3 0 0 0 17 272 39 0.0 3 0 0 1 38 273 39 0.0 3 0 0 2 37 274 5 0.0 3 0 0 0 5 275 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 2 0.0 3 0 0 0 2 278 3 0.0 3 0 0 0 3 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 9 0.0 3 0 0 0 9 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 291 2 0.0 3 0 0 0 2 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 12,935 Reads with adapters: 185 (1.4%) Reads written (passing filters): 12,935 (100.0%) Total basepairs processed: 2,213,545 bp Total written (filtered): 2,212,978 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 185 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.2% C: 18.4% G: 15.7% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 174 202.1 0 174 4 10 50.5 0 10 5 1 12.6 0 1