This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.84 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 192,694 (8.8%) Reads that were too short: 17,062 (0.8%) Reads written (passing filters): 175,632 (8.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 37,037,250 bp (5.6%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 192694 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1815 535.2 0 1815 7 3495 535.2 0 3495 8 156 535.2 0 156 9 2723 535.2 0 2723 10 51 535.2 0 51 11 70 535.2 0 70 12 130 535.2 0 130 13 66 535.2 0 66 14 66 535.2 0 66 15 95 535.2 0 95 16 99 535.2 0 99 17 65 535.2 0 65 18 73 535.2 0 73 19 68 535.2 0 68 20 80 535.2 0 80 21 81 535.2 0 81 22 62 535.2 0 62 23 63 535.2 0 63 24 96 535.2 0 96 25 86 535.2 0 86 26 55 535.2 0 55 27 87 535.2 0 87 28 87 535.2 0 87 29 58 535.2 0 58 30 48 535.2 0 48 31 64 535.2 0 64 32 74 535.2 0 74 33 84 535.2 0 84 34 85 535.2 0 85 35 94 535.2 0 94 36 94 535.2 0 94 37 71 535.2 0 71 38 79 535.2 0 79 39 79 535.2 0 79 40 99 535.2 0 99 41 83 535.2 0 83 42 97 535.2 0 97 43 96 535.2 0 96 44 81 535.2 0 81 45 105 535.2 0 105 46 91 535.2 0 91 47 89 535.2 0 89 48 96 535.2 0 96 49 85 535.2 0 85 50 58 535.2 0 58 51 74 535.2 0 74 52 61 535.2 0 61 53 92 535.2 0 92 54 132 535.2 0 132 55 120 535.2 0 120 56 91 535.2 0 91 57 118 535.2 0 118 58 73 535.2 0 73 59 82 535.2 0 82 60 113 535.2 0 113 61 83 535.2 0 83 62 181 535.2 0 181 63 114 535.2 0 114 64 118 535.2 0 118 65 91 535.2 0 91 66 136 535.2 0 136 67 93 535.2 0 93 68 128 535.2 0 128 69 172 535.2 0 172 70 183 535.2 0 183 71 244 535.2 0 244 72 623 535.2 0 623 73 1131 535.2 0 1131 74 386 535.2 0 386 75 392 535.2 0 392 76 710 535.2 0 710 77 358 535.2 0 358 78 321 535.2 0 321 79 703 535.2 0 703 80 737 535.2 0 737 81 830 535.2 0 830 82 832 535.2 0 832 83 1084 535.2 0 1084 84 1315 535.2 0 1315 85 2162 535.2 0 2162 86 30700 535.2 0 30700 87 53031 535.2 0 53031 88 42914 535.2 0 42914 89 201 535.2 0 201 90 72 535.2 0 72 91 44 535.2 0 44 92 70 535.2 0 70 93 58 535.2 0 58 94 66 535.2 0 66 95 67 535.2 0 67 96 74 535.2 0 74 97 65 535.2 0 65 98 58 535.2 0 58 99 73 535.2 0 73 100 77 535.2 0 77 101 72 535.2 0 72 102 81 535.2 0 81 103 215 535.2 0 215 104 232 535.2 0 232 105 175 535.2 0 175 106 47 535.2 0 47 107 45 535.2 0 45 108 72 535.2 0 72 109 44 535.2 0 44 110 74 535.2 0 74 111 73 535.2 0 73 112 62 535.2 0 62 113 68 535.2 0 68 114 64 535.2 0 64 115 89 535.2 0 89 116 71 535.2 0 71 117 62 535.2 0 62 118 78 535.2 0 78 119 83 535.2 0 83 120 75 535.2 0 75 121 78 535.2 0 78 122 99 535.2 0 99 123 249 535.2 0 249 124 455 535.2 0 455 125 928 535.2 0 928 126 572 535.2 0 572 127 282 535.2 0 282 128 132 535.2 0 132 129 108 535.2 0 108 130 141 535.2 0 141 131 160 535.2 0 160 132 110 535.2 0 110 133 100 535.2 0 100 134 135 535.2 0 135 135 108 535.2 0 108 136 159 535.2 0 159 137 106 535.2 0 106 138 112 535.2 0 112 139 122 535.2 0 122 140 104 535.2 0 104 141 219 535.2 0 219 142 202 535.2 0 202 143 211 535.2 0 211 144 1102 535.2 0 1102 145 1247 535.2 0 1247 146 3338 535.2 0 3338 147 280 535.2 0 280 148 81 535.2 0 81 149 124 535.2 0 124 150 145 535.2 0 145 151 135 535.2 0 135 152 74 535.2 0 74 153 71 535.2 0 71 154 80 535.2 0 80 155 112 535.2 0 112 156 122 535.2 0 122 157 80 535.2 0 80 158 90 535.2 0 90 159 61 535.2 0 61 160 67 535.2 0 67 161 70 535.2 0 70 162 56 535.2 0 56 163 71 535.2 0 71 164 72 535.2 0 72 165 95 535.2 0 95 166 381 535.2 0 381 167 207 535.2 0 207 168 211 535.2 0 211 169 65 535.2 0 65 170 93 535.2 0 93 171 91 535.2 0 91 172 119 535.2 0 119 173 83 535.2 0 83 174 83 535.2 0 83 175 125 535.2 0 125 176 98 535.2 0 98 177 73 535.2 0 73 178 73 535.2 0 73 179 64 535.2 0 64 180 105 535.2 0 105 181 105 535.2 0 105 182 137 535.2 0 137 183 132 535.2 0 132 184 153 535.2 0 153 185 908 535.2 0 908 186 1553 535.2 0 1553 187 1955 535.2 0 1955 188 157 535.2 0 157 189 57 535.2 0 57 190 69 535.2 0 69 191 71 535.2 0 71 192 103 535.2 0 103 193 153 535.2 0 153 194 146 535.2 0 146 195 159 535.2 0 159 196 110 535.2 0 110 197 75 535.2 0 75 198 64 535.2 0 64 199 74 535.2 0 74 200 112 535.2 0 112 201 89 535.2 0 89 202 88 535.2 0 88 203 82 535.2 0 82 204 92 535.2 0 92 205 123 535.2 0 123 206 823 535.2 0 823 207 925 535.2 0 925 208 1422 535.2 0 1422 209 104 535.2 0 104 210 193 535.2 0 193 211 458 535.2 0 458 212 649 535.2 0 649 213 369 535.2 0 369 214 84 535.2 0 84 215 56 535.2 0 56 216 70 535.2 0 70 217 77 535.2 0 77 218 69 535.2 0 69 219 75 535.2 0 75 220 100 535.2 0 100 221 93 535.2 0 93 222 107 535.2 0 107 223 52 535.2 0 52 224 85 535.2 0 85 225 72 535.2 0 72 226 129 535.2 0 129 227 148 535.2 0 148 228 201 535.2 0 201 229 99 535.2 0 99 230 65 535.2 0 65 231 100 535.2 0 100 232 74 535.2 0 74 233 68 535.2 0 68 234 84 535.2 0 84 235 61 535.2 0 61 236 53 535.2 0 53 237 68 535.2 0 68 238 70 535.2 0 70 239 70 535.2 0 70 240 78 535.2 0 78 241 106 535.2 0 106 242 131 535.2 0 131 243 131 535.2 0 131 244 79 535.2 0 79 245 97 535.2 0 97 246 165 535.2 0 165 247 91 535.2 0 91 248 88 535.2 0 88 249 66 535.2 0 66 250 86 535.2 0 86 251 72 535.2 0 72 252 78 535.2 0 78 253 61 535.2 0 61 254 70 535.2 0 70 255 175 535.2 0 175 256 186 535.2 0 186 257 207 535.2 0 207 258 119 535.2 0 119 259 667 535.2 0 667 260 1062 535.2 0 1062 261 1485 535.2 0 1485 262 246 535.2 0 246 263 77 535.2 0 77 264 77 535.2 0 77 265 101 535.2 0 101 266 102 535.2 0 102 267 212 535.2 0 212 268 230 535.2 0 230 269 145 535.2 0 145 270 80 535.2 0 80 271 56 535.2 0 56 272 77 535.2 0 77 273 66 535.2 0 66 274 65 535.2 0 65 275 124 535.2 0 124 276 142 535.2 0 142 277 103 535.2 0 103 278 85 535.2 0 85 279 97 535.2 0 97 280 130 535.2 0 130 281 160 535.2 0 160 282 135 535.2 0 135 283 123 535.2 0 123 284 51 535.2 0 51 285 78 535.2 0 78 286 46 535.2 0 46 287 73 535.2 0 73 288 70 535.2 0 70 289 95 535.2 0 95 290 66 535.2 0 66 291 70 535.2 0 70 292 67 535.2 0 67 293 69 535.2 0 69 294 138 535.2 0 138 295 110 535.2 0 110 296 117 535.2 0 117 297 73 535.2 0 73 298 66 535.2 0 66 299 61 535.2 0 61 300 93 535.2 0 93 301 228 535.2 0 228 Finished in 6.62 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 175,632 Reads with adapters: 7,815 (4.4%) Reads that were too short: 493 (0.3%) Reads written (passing filters): 7,322 (4.2%) Total basepairs processed: 37,037,250 bp Total written (filtered): 1,493,708 bp (4.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7815 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 1 0.0 3 0 0 1 16 28 0.0 3 0 21 5 2 17 2708 0.0 3 2472 192 38 6 18 35 0.0 3 5 22 2 6 19 1333 0.0 3 1196 108 18 11 20 24 0.0 3 10 7 0 7 21 16 0.0 3 0 0 4 12 22 9 0.0 3 0 0 0 9 23 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 4 0.0 3 0 0 0 4 37 13 0.0 3 0 0 0 13 40 2 0.0 3 0 0 1 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 2 0.0 3 0 0 0 2 51 5 0.0 3 0 0 0 5 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 1 64 1 0.0 3 0 0 0 1 66 27 0.0 3 0 0 0 27 67 1 0.0 3 0 0 0 1 68 7 0.0 3 0 0 0 7 69 7 0.0 3 0 0 1 6 71 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 101 0.0 3 0 0 0 101 81 11 0.0 3 0 0 0 11 82 13 0.0 3 0 0 0 13 83 25 0.0 3 0 0 0 25 84 3 0.0 3 0 0 0 3 85 3 0.0 3 0 0 0 3 86 4 0.0 3 0 0 0 4 87 1 0.0 3 0 0 0 1 88 7 0.0 3 0 0 0 7 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 1 1 92 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 1 99 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 9 0.0 3 0 0 0 9 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 28 0.0 3 0 0 0 28 105 2074 0.0 3 0 0 27 2047 106 563 0.0 3 0 0 15 548 107 8 0.0 3 0 0 0 8 108 199 0.0 3 0 0 14 185 109 12 0.0 3 0 0 0 12 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 121 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 4 0.0 3 0 0 0 4 130 1 0.0 3 0 0 0 1 131 34 0.0 3 0 1 1 32 132 2 0.0 3 0 0 1 1 133 3 0.0 3 0 0 0 3 138 3 0.0 3 0 0 0 3 139 3 0.0 3 0 0 0 3 140 22 0.0 3 0 0 0 22 142 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 153 4 0.0 3 0 0 0 4 154 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 44 0.0 3 0 0 1 43 157 4 0.0 3 0 0 1 3 158 17 0.0 3 0 0 0 17 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 12 0.0 3 0 0 0 12 166 9 0.0 3 0 0 2 7 167 1 0.0 3 0 0 1 168 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 0 3 171 2 0.0 3 0 0 0 2 177 2 0.0 3 0 0 0 2 178 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 181 16 0.0 3 0 0 0 16 182 6 0.0 3 0 0 0 6 183 5 0.0 3 0 0 1 4 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 4 0.0 3 0 0 0 4 194 2 0.0 3 0 0 0 2 195 7 0.0 3 0 0 0 7 196 5 0.0 3 0 0 0 5 197 14 0.0 3 0 0 2 12 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 206 110 0.0 3 0 0 4 106 207 61 0.0 3 0 0 5 56 208 5 0.0 3 0 0 0 5 209 3 0.0 3 0 0 0 3 210 1 0.0 3 0 0 0 1 212 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 7,322 Reads with adapters: 39 (0.5%) Reads written (passing filters): 7,322 (100.0%) Total basepairs processed: 1,493,708 bp Total written (filtered): 1,493,582 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 39 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.4% C: 10.3% G: 56.4% T: 17.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 31 114.4 0 31 4 7 28.6 0 7 5 1 7.2 0 1