This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.53 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 110,103 (5.0%) Reads that were too short: 36,940 (1.7%) Reads written (passing filters): 73,163 (3.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 16,057,359 bp (2.4%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 110103 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3393 535.2 0 3393 7 2146 535.2 0 2146 8 7115 535.2 0 7115 9 6151 535.2 0 6151 10 110 535.2 0 110 11 116 535.2 0 116 12 103 535.2 0 103 13 112 535.2 0 112 14 116 535.2 0 116 15 163 535.2 0 163 16 146 535.2 0 146 17 131 535.2 0 131 18 117 535.2 0 117 19 139 535.2 0 139 20 133 535.2 0 133 21 121 535.2 0 121 22 105 535.2 0 105 23 106 535.2 0 106 24 125 535.2 0 125 25 104 535.2 0 104 26 138 535.2 0 138 27 157 535.2 0 157 28 135 535.2 0 135 29 124 535.2 0 124 30 135 535.2 0 135 31 141 535.2 0 141 32 164 535.2 0 164 33 194 535.2 0 194 34 142 535.2 0 142 35 194 535.2 0 194 36 171 535.2 0 171 37 1570 535.2 0 1570 38 2521 535.2 0 2521 39 3571 535.2 0 3571 40 160 535.2 0 160 41 180 535.2 0 180 42 150 535.2 0 150 43 152 535.2 0 152 44 146 535.2 0 146 45 126 535.2 0 126 46 103 535.2 0 103 47 117 535.2 0 117 48 128 535.2 0 128 49 163 535.2 0 163 50 116 535.2 0 116 51 131 535.2 0 131 52 119 535.2 0 119 53 120 535.2 0 120 54 144 535.2 0 144 55 116 535.2 0 116 56 123 535.2 0 123 57 129 535.2 0 129 58 119 535.2 0 119 59 124 535.2 0 124 60 131 535.2 0 131 61 112 535.2 0 112 62 128 535.2 0 128 63 150 535.2 0 150 64 113 535.2 0 113 65 108 535.2 0 108 66 135 535.2 0 135 67 93 535.2 0 93 68 112 535.2 0 112 69 135 535.2 0 135 70 158 535.2 0 158 71 153 535.2 0 153 72 187 535.2 0 187 73 128 535.2 0 128 74 141 535.2 0 141 75 116 535.2 0 116 76 184 535.2 0 184 77 154 535.2 0 154 78 152 535.2 0 152 79 181 535.2 0 181 80 160 535.2 0 160 81 105 535.2 0 105 82 153 535.2 0 153 83 108 535.2 0 108 84 116 535.2 0 116 85 103 535.2 0 103 86 101 535.2 0 101 87 103 535.2 0 103 88 136 535.2 0 136 89 168 535.2 0 168 90 170 535.2 0 170 91 392 535.2 0 392 92 687 535.2 0 687 93 1653 535.2 0 1653 94 894 535.2 0 894 95 190 535.2 0 190 96 146 535.2 0 146 97 161 535.2 0 161 98 113 535.2 0 113 99 110 535.2 0 110 100 120 535.2 0 120 101 131 535.2 0 131 102 100 535.2 0 100 103 132 535.2 0 132 104 196 535.2 0 196 105 202 535.2 0 202 106 150 535.2 0 150 107 122 535.2 0 122 108 133 535.2 0 133 109 160 535.2 0 160 110 147 535.2 0 147 111 137 535.2 0 137 112 217 535.2 0 217 113 227 535.2 0 227 114 333 535.2 0 333 115 289 535.2 0 289 116 172 535.2 0 172 117 164 535.2 0 164 118 183 535.2 0 183 119 115 535.2 0 115 120 130 535.2 0 130 121 140 535.2 0 140 122 179 535.2 0 179 123 153 535.2 0 153 124 148 535.2 0 148 125 145 535.2 0 145 126 135 535.2 0 135 127 226 535.2 0 226 128 272 535.2 0 272 129 604 535.2 0 604 130 770 535.2 0 770 131 840 535.2 0 840 132 238 535.2 0 238 133 192 535.2 0 192 134 221 535.2 0 221 135 203 535.2 0 203 136 311 535.2 0 311 137 275 535.2 0 275 138 276 535.2 0 276 139 274 535.2 0 274 140 291 535.2 0 291 141 315 535.2 0 315 142 3230 535.2 0 3230 143 3950 535.2 0 3950 144 7513 535.2 0 7513 145 187 535.2 0 187 146 118 535.2 0 118 147 136 535.2 0 136 148 130 535.2 0 130 149 182 535.2 0 182 150 216 535.2 0 216 151 165 535.2 0 165 152 112 535.2 0 112 153 148 535.2 0 148 154 137 535.2 0 137 155 124 535.2 0 124 156 230 535.2 0 230 157 115 535.2 0 115 158 124 535.2 0 124 159 122 535.2 0 122 160 119 535.2 0 119 161 122 535.2 0 122 162 127 535.2 0 127 163 127 535.2 0 127 164 137 535.2 0 137 165 101 535.2 0 101 166 108 535.2 0 108 167 96 535.2 0 96 168 150 535.2 0 150 169 165 535.2 0 165 170 191 535.2 0 191 171 93 535.2 0 93 172 90 535.2 0 90 173 119 535.2 0 119 174 129 535.2 0 129 175 93 535.2 0 93 176 124 535.2 0 124 177 171 535.2 0 171 178 162 535.2 0 162 179 173 535.2 0 173 180 108 535.2 0 108 181 80 535.2 0 80 182 144 535.2 0 144 183 97 535.2 0 97 184 91 535.2 0 91 185 134 535.2 0 134 186 132 535.2 0 132 187 116 535.2 0 116 188 102 535.2 0 102 189 117 535.2 0 117 190 131 535.2 0 131 191 148 535.2 0 148 192 138 535.2 0 138 193 108 535.2 0 108 194 109 535.2 0 109 195 102 535.2 0 102 196 86 535.2 0 86 197 110 535.2 0 110 198 108 535.2 0 108 199 114 535.2 0 114 200 103 535.2 0 103 201 166 535.2 0 166 202 152 535.2 0 152 203 160 535.2 0 160 204 175 535.2 0 175 205 156 535.2 0 156 206 139 535.2 0 139 207 118 535.2 0 118 208 153 535.2 0 153 209 317 535.2 0 317 210 350 535.2 0 350 211 456 535.2 0 456 212 377 535.2 0 377 213 335 535.2 0 335 214 2434 535.2 0 2434 215 3212 535.2 0 3212 216 3907 535.2 0 3907 217 310 535.2 0 310 218 214 535.2 0 214 219 113 535.2 0 113 220 133 535.2 0 133 221 160 535.2 0 160 222 135 535.2 0 135 223 143 535.2 0 143 224 160 535.2 0 160 225 155 535.2 0 155 226 162 535.2 0 162 227 180 535.2 0 180 228 294 535.2 0 294 229 546 535.2 0 546 230 522 535.2 0 522 231 623 535.2 0 623 232 222 535.2 0 222 233 292 535.2 0 292 234 1699 535.2 0 1699 235 3216 535.2 0 3216 236 3520 535.2 0 3520 237 963 535.2 0 963 238 111 535.2 0 111 239 97 535.2 0 97 240 82 535.2 0 82 241 87 535.2 0 87 242 76 535.2 0 76 243 119 535.2 0 119 244 121 535.2 0 121 245 103 535.2 0 103 246 88 535.2 0 88 247 109 535.2 0 109 248 90 535.2 0 90 249 107 535.2 0 107 250 102 535.2 0 102 251 86 535.2 0 86 252 84 535.2 0 84 253 115 535.2 0 115 254 136 535.2 0 136 255 137 535.2 0 137 256 143 535.2 0 143 257 82 535.2 0 82 258 90 535.2 0 90 259 98 535.2 0 98 260 121 535.2 0 121 261 118 535.2 0 118 262 134 535.2 0 134 263 171 535.2 0 171 264 134 535.2 0 134 265 121 535.2 0 121 266 118 535.2 0 118 267 100 535.2 0 100 268 83 535.2 0 83 269 80 535.2 0 80 270 124 535.2 0 124 271 176 535.2 0 176 272 179 535.2 0 179 273 82 535.2 0 82 274 96 535.2 0 96 275 129 535.2 0 129 276 101 535.2 0 101 277 79 535.2 0 79 278 78 535.2 0 78 279 91 535.2 0 91 280 84 535.2 0 84 281 114 535.2 0 114 282 123 535.2 0 123 283 160 535.2 0 160 284 208 535.2 0 208 285 127 535.2 0 127 286 94 535.2 0 94 287 84 535.2 0 84 288 78 535.2 0 78 289 103 535.2 0 103 290 90 535.2 0 90 291 213 535.2 0 213 292 248 535.2 0 248 293 322 535.2 0 322 294 100 535.2 0 100 295 186 535.2 0 186 296 442 535.2 0 442 297 719 535.2 0 719 298 997 535.2 0 997 299 658 535.2 0 658 300 179 535.2 0 179 301 830 535.2 0 830 Finished in 3.08 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 73,163 Reads with adapters: 10,347 (14.1%) Reads that were too short: 124 (0.2%) Reads written (passing filters): 10,223 (14.0%) Total basepairs processed: 16,057,359 bp Total written (filtered): 2,791,695 bp (17.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10347 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 11 0.0 3 0 0 6 5 16 81 0.0 3 0 58 15 8 17 6951 0.0 3 6251 559 102 39 18 2888 0.0 3 2637 193 44 14 19 35 0.0 3 0 7 14 14 20 28 0.0 3 0 0 5 23 21 14 0.0 3 0 0 0 14 25 1 0.0 3 1 30 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 42 4 0.0 3 0 0 0 4 45 5 0.0 3 0 0 0 5 46 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 33 0.0 3 0 0 1 32 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 7 0.0 3 0 0 1 6 55 2 0.0 3 0 0 0 2 57 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 63 19 0.0 3 0 0 1 18 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 0 4 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 4 0.0 3 0 0 0 4 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 12 0.0 3 0 0 0 12 100 4 0.0 3 0 0 0 4 101 14 0.0 3 0 0 0 14 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 3 0.0 3 0 0 0 3 111 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 1 2 132 4 0.0 3 0 0 0 4 133 7 0.0 3 0 0 0 7 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 151 8 0.0 3 0 0 0 8 152 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 1 1 154 23 0.0 3 0 0 0 23 155 6 0.0 3 0 0 0 6 156 3 0.0 3 0 0 0 3 157 4 0.0 3 0 0 1 3 158 5 0.0 3 0 0 0 5 159 3 0.0 3 0 0 1 2 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 1 180 5 0.0 3 0 0 0 5 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 6 0.0 3 0 0 1 5 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 205 5 0.0 3 0 0 0 5 206 3 0.0 3 0 0 0 3 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 215 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 256 8 0.0 3 0 0 0 8 261 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 10,223 Reads with adapters: 66 (0.6%) Reads written (passing filters): 10,223 (100.0%) Total basepairs processed: 2,791,695 bp Total written (filtered): 2,791,479 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 66 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.4% C: 16.7% G: 27.3% T: 13.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 52 159.7 0 52 4 10 39.9 0 10 5 4 10.0 0 4