This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.23 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 80,621 (3.7%) Reads that were too short: 16,956 (0.8%) Reads written (passing filters): 63,665 (2.9%) Total basepairs processed: 659,880,795 bp Total written (filtered): 13,945,053 bp (2.1%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 80621 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3067 535.2 0 3067 7 2563 535.2 0 2563 8 6953 535.2 0 6953 9 7130 535.2 0 7130 10 94 535.2 0 94 11 94 535.2 0 94 12 76 535.2 0 76 13 87 535.2 0 87 14 88 535.2 0 88 15 102 535.2 0 102 16 97 535.2 0 97 17 93 535.2 0 93 18 88 535.2 0 88 19 103 535.2 0 103 20 93 535.2 0 93 21 86 535.2 0 86 22 93 535.2 0 93 23 92 535.2 0 92 24 101 535.2 0 101 25 78 535.2 0 78 26 121 535.2 0 121 27 141 535.2 0 141 28 109 535.2 0 109 29 74 535.2 0 74 30 103 535.2 0 103 31 108 535.2 0 108 32 95 535.2 0 95 33 147 535.2 0 147 34 133 535.2 0 133 35 131 535.2 0 131 36 118 535.2 0 118 37 1091 535.2 0 1091 38 1002 535.2 0 1002 39 1722 535.2 0 1722 40 97 535.2 0 97 41 105 535.2 0 105 42 123 535.2 0 123 43 110 535.2 0 110 44 103 535.2 0 103 45 110 535.2 0 110 46 85 535.2 0 85 47 115 535.2 0 115 48 102 535.2 0 102 49 112 535.2 0 112 50 96 535.2 0 96 51 84 535.2 0 84 52 105 535.2 0 105 53 95 535.2 0 95 54 87 535.2 0 87 55 90 535.2 0 90 56 103 535.2 0 103 57 91 535.2 0 91 58 98 535.2 0 98 59 98 535.2 0 98 60 74 535.2 0 74 61 78 535.2 0 78 62 101 535.2 0 101 63 94 535.2 0 94 64 83 535.2 0 83 65 94 535.2 0 94 66 89 535.2 0 89 67 95 535.2 0 95 68 73 535.2 0 73 69 86 535.2 0 86 70 139 535.2 0 139 71 116 535.2 0 116 72 123 535.2 0 123 73 82 535.2 0 82 74 98 535.2 0 98 75 82 535.2 0 82 76 132 535.2 0 132 77 93 535.2 0 93 78 96 535.2 0 96 79 120 535.2 0 120 80 89 535.2 0 89 81 75 535.2 0 75 82 92 535.2 0 92 83 109 535.2 0 109 84 74 535.2 0 74 85 67 535.2 0 67 86 84 535.2 0 84 87 95 535.2 0 95 88 92 535.2 0 92 89 104 535.2 0 104 90 89 535.2 0 89 91 241 535.2 0 241 92 245 535.2 0 245 93 995 535.2 0 995 94 310 535.2 0 310 95 91 535.2 0 91 96 101 535.2 0 101 97 130 535.2 0 130 98 80 535.2 0 80 99 71 535.2 0 71 100 82 535.2 0 82 101 90 535.2 0 90 102 94 535.2 0 94 103 87 535.2 0 87 104 216 535.2 0 216 105 204 535.2 0 204 106 98 535.2 0 98 107 109 535.2 0 109 108 88 535.2 0 88 109 114 535.2 0 114 110 102 535.2 0 102 111 94 535.2 0 94 112 159 535.2 0 159 113 102 535.2 0 102 114 193 535.2 0 193 115 179 535.2 0 179 116 142 535.2 0 142 117 115 535.2 0 115 118 118 535.2 0 118 119 94 535.2 0 94 120 85 535.2 0 85 121 94 535.2 0 94 122 120 535.2 0 120 123 100 535.2 0 100 124 119 535.2 0 119 125 98 535.2 0 98 126 100 535.2 0 100 127 185 535.2 0 185 128 214 535.2 0 214 129 565 535.2 0 565 130 918 535.2 0 918 131 895 535.2 0 895 132 234 535.2 0 234 133 168 535.2 0 168 134 142 535.2 0 142 135 165 535.2 0 165 136 216 535.2 0 216 137 245 535.2 0 245 138 226 535.2 0 226 139 206 535.2 0 206 140 266 535.2 0 266 141 304 535.2 0 304 142 3633 535.2 0 3633 143 4653 535.2 0 4653 144 8623 535.2 0 8623 145 151 535.2 0 151 146 88 535.2 0 88 147 74 535.2 0 74 148 74 535.2 0 74 149 106 535.2 0 106 150 121 535.2 0 121 151 107 535.2 0 107 152 88 535.2 0 88 153 119 535.2 0 119 154 97 535.2 0 97 155 96 535.2 0 96 156 259 535.2 0 259 157 96 535.2 0 96 158 81 535.2 0 81 159 95 535.2 0 95 160 70 535.2 0 70 161 70 535.2 0 70 162 72 535.2 0 72 163 86 535.2 0 86 164 92 535.2 0 92 165 76 535.2 0 76 166 104 535.2 0 104 167 93 535.2 0 93 168 90 535.2 0 90 169 120 535.2 0 120 170 126 535.2 0 126 171 70 535.2 0 70 172 66 535.2 0 66 173 105 535.2 0 105 174 72 535.2 0 72 175 65 535.2 0 65 176 86 535.2 0 86 177 118 535.2 0 118 178 113 535.2 0 113 179 103 535.2 0 103 180 76 535.2 0 76 181 68 535.2 0 68 182 96 535.2 0 96 183 91 535.2 0 91 184 71 535.2 0 71 185 72 535.2 0 72 186 70 535.2 0 70 187 71 535.2 0 71 188 80 535.2 0 80 189 71 535.2 0 71 190 94 535.2 0 94 191 117 535.2 0 117 192 106 535.2 0 106 193 58 535.2 0 58 194 58 535.2 0 58 195 62 535.2 0 62 196 57 535.2 0 57 197 70 535.2 0 70 198 72 535.2 0 72 199 89 535.2 0 89 200 62 535.2 0 62 201 87 535.2 0 87 202 85 535.2 0 85 203 97 535.2 0 97 204 99 535.2 0 99 205 82 535.2 0 82 206 88 535.2 0 88 207 85 535.2 0 85 208 74 535.2 0 74 209 143 535.2 0 143 210 126 535.2 0 126 211 145 535.2 0 145 212 176 535.2 0 176 213 119 535.2 0 119 214 596 535.2 0 596 215 864 535.2 0 864 216 908 535.2 0 908 217 126 535.2 0 126 218 124 535.2 0 124 219 79 535.2 0 79 220 85 535.2 0 85 221 91 535.2 0 91 222 92 535.2 0 92 223 85 535.2 0 85 224 99 535.2 0 99 225 85 535.2 0 85 226 78 535.2 0 78 227 116 535.2 0 116 228 162 535.2 0 162 229 282 535.2 0 282 230 256 535.2 0 256 231 297 535.2 0 297 232 118 535.2 0 118 233 125 535.2 0 125 234 629 535.2 0 629 235 1231 535.2 0 1231 236 1396 535.2 0 1396 237 494 535.2 0 494 238 78 535.2 0 78 239 67 535.2 0 67 240 63 535.2 0 63 241 80 535.2 0 80 242 47 535.2 0 47 243 67 535.2 0 67 244 91 535.2 0 91 245 92 535.2 0 92 246 84 535.2 0 84 247 73 535.2 0 73 248 49 535.2 0 49 249 66 535.2 0 66 250 77 535.2 0 77 251 63 535.2 0 63 252 65 535.2 0 65 253 70 535.2 0 70 254 86 535.2 0 86 255 72 535.2 0 72 256 64 535.2 0 64 257 59 535.2 0 59 258 89 535.2 0 89 259 77 535.2 0 77 260 90 535.2 0 90 261 70 535.2 0 70 262 110 535.2 0 110 263 97 535.2 0 97 264 101 535.2 0 101 265 89 535.2 0 89 266 72 535.2 0 72 267 57 535.2 0 57 268 84 535.2 0 84 269 56 535.2 0 56 270 96 535.2 0 96 271 109 535.2 0 109 272 91 535.2 0 91 273 61 535.2 0 61 274 74 535.2 0 74 275 66 535.2 0 66 276 58 535.2 0 58 277 68 535.2 0 68 278 87 535.2 0 87 279 80 535.2 0 80 280 67 535.2 0 67 281 73 535.2 0 73 282 83 535.2 0 83 283 98 535.2 0 98 284 83 535.2 0 83 285 78 535.2 0 78 286 58 535.2 0 58 287 55 535.2 0 55 288 88 535.2 0 88 289 69 535.2 0 69 290 69 535.2 0 69 291 153 535.2 0 153 292 177 535.2 0 177 293 176 535.2 0 176 294 81 535.2 0 81 295 129 535.2 0 129 296 233 535.2 0 233 297 359 535.2 0 359 298 510 535.2 0 510 299 274 535.2 0 274 300 114 535.2 0 114 301 997 535.2 0 997 Finished in 2.86 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 63,665 Reads with adapters: 9,910 (15.6%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 9,902 (15.6%) Total basepairs processed: 13,945,053 bp Total written (filtered): 2,682,941 bp (19.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9910 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 3 1 19 59 0.0 3 0 58 1 20 7343 0.0 4 7209 131 2 1 21 2271 0.0 4 2223 47 1 22 8 0.0 4 2 6 23 90 0.0 4 82 2 1 5 25 1 0.0 4 1 26 1 0.0 4 1 27 1 0.0 4 1 42 21 0.0 4 0 0 0 0 21 45 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 97 12 0.0 4 0 0 0 0 12 111 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 77 0.0 4 0 0 0 0 77 128 5 0.0 4 0 0 0 0 5 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 9,902 Reads with adapters: 1,480 (14.9%) Reads written (passing filters): 9,902 (100.0%) Total basepairs processed: 2,682,941 bp Total written (filtered): 2,667,476 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1480 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 3.8% G: 94.2% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 154.7 0 6 4 22 38.7 0 22 5 1 9.7 0 1 8 2 0.2 0 2 9 625 0.0 0 21 604 10 456 0.0 1 21 435 11 360 0.0 1 2 358 12 1 0.0 1 0 1 13 1 0.0 1 0 1 14 1 0.0 1 0 1 150 1 0.0 1 0 1 209 1 0.0 1 0 1 257 1 0.0 1 1 269 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.