This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.31 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 68,262 (3.1%) Reads that were too short: 12,168 (0.6%) Reads written (passing filters): 56,094 (2.6%) Total basepairs processed: 659,880,795 bp Total written (filtered): 13,009,922 bp (2.0%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 68262 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 346 535.2 0 346 7 17098 535.2 0 17098 8 13067 535.2 0 13067 9 59 535.2 0 59 10 35 535.2 0 35 11 31 535.2 0 31 12 58 535.2 0 58 13 34 535.2 0 34 14 39 535.2 0 39 15 35 535.2 0 35 16 62 535.2 0 62 17 41 535.2 0 41 18 49 535.2 0 49 19 52 535.2 0 52 20 61 535.2 0 61 21 51 535.2 0 51 22 84 535.2 0 84 23 45 535.2 0 45 24 35 535.2 0 35 25 37 535.2 0 37 26 47 535.2 0 47 27 77 535.2 0 77 28 66 535.2 0 66 29 68 535.2 0 68 30 214 535.2 0 214 31 71 535.2 0 71 32 68 535.2 0 68 33 32 535.2 0 32 34 41 535.2 0 41 35 35 535.2 0 35 36 41 535.2 0 41 37 51 535.2 0 51 38 41 535.2 0 41 39 40 535.2 0 40 40 60 535.2 0 60 41 43 535.2 0 43 42 36 535.2 0 36 43 34 535.2 0 34 44 58 535.2 0 58 45 39 535.2 0 39 46 56 535.2 0 56 47 38 535.2 0 38 48 35 535.2 0 35 49 42 535.2 0 42 50 37 535.2 0 37 51 34 535.2 0 34 52 66 535.2 0 66 53 48 535.2 0 48 54 93 535.2 0 93 55 144 535.2 0 144 56 179 535.2 0 179 57 34 535.2 0 34 58 37 535.2 0 37 59 47 535.2 0 47 60 66 535.2 0 66 61 379 535.2 0 379 62 384 535.2 0 384 63 653 535.2 0 653 64 49 535.2 0 49 65 40 535.2 0 40 66 31 535.2 0 31 67 96 535.2 0 96 68 109 535.2 0 109 69 146 535.2 0 146 70 44 535.2 0 44 71 60 535.2 0 60 72 110 535.2 0 110 73 117 535.2 0 117 74 54 535.2 0 54 75 41 535.2 0 41 76 52 535.2 0 52 77 38 535.2 0 38 78 83 535.2 0 83 79 87 535.2 0 87 80 142 535.2 0 142 81 52 535.2 0 52 82 42 535.2 0 42 83 64 535.2 0 64 84 63 535.2 0 63 85 55 535.2 0 55 86 75 535.2 0 75 87 81 535.2 0 81 88 70 535.2 0 70 89 40 535.2 0 40 90 43 535.2 0 43 91 40 535.2 0 40 92 35 535.2 0 35 93 34 535.2 0 34 94 42 535.2 0 42 95 37 535.2 0 37 96 53 535.2 0 53 97 47 535.2 0 47 98 66 535.2 0 66 99 33 535.2 0 33 100 44 535.2 0 44 101 42 535.2 0 42 102 31 535.2 0 31 103 34 535.2 0 34 104 22 535.2 0 22 105 35 535.2 0 35 106 53 535.2 0 53 107 41 535.2 0 41 108 39 535.2 0 39 109 38 535.2 0 38 110 36 535.2 0 36 111 55 535.2 0 55 112 63 535.2 0 63 113 61 535.2 0 61 114 74 535.2 0 74 115 71 535.2 0 71 116 53 535.2 0 53 117 44 535.2 0 44 118 31 535.2 0 31 119 55 535.2 0 55 120 37 535.2 0 37 121 54 535.2 0 54 122 61 535.2 0 61 123 113 535.2 0 113 124 117 535.2 0 117 125 169 535.2 0 169 126 162 535.2 0 162 127 185 535.2 0 185 128 301 535.2 0 301 129 502 535.2 0 502 130 453 535.2 0 453 131 256 535.2 0 256 132 74 535.2 0 74 133 60 535.2 0 60 134 160 535.2 0 160 135 101 535.2 0 101 136 107 535.2 0 107 137 56 535.2 0 56 138 63 535.2 0 63 139 78 535.2 0 78 140 74 535.2 0 74 141 58 535.2 0 58 142 51 535.2 0 51 143 60 535.2 0 60 144 191 535.2 0 191 145 410 535.2 0 410 146 307 535.2 0 307 147 198 535.2 0 198 148 62 535.2 0 62 149 161 535.2 0 161 150 50 535.2 0 50 151 54 535.2 0 54 152 63 535.2 0 63 153 35 535.2 0 35 154 39 535.2 0 39 155 54 535.2 0 54 156 153 535.2 0 153 157 175 535.2 0 175 158 60 535.2 0 60 159 60 535.2 0 60 160 48 535.2 0 48 161 35 535.2 0 35 162 46 535.2 0 46 163 34 535.2 0 34 164 41 535.2 0 41 165 69 535.2 0 69 166 249 535.2 0 249 167 164 535.2 0 164 168 195 535.2 0 195 169 39 535.2 0 39 170 37 535.2 0 37 171 53 535.2 0 53 172 51 535.2 0 51 173 35 535.2 0 35 174 66 535.2 0 66 175 70 535.2 0 70 176 71 535.2 0 71 177 48 535.2 0 48 178 60 535.2 0 60 179 45 535.2 0 45 180 55 535.2 0 55 181 42 535.2 0 42 182 81 535.2 0 81 183 70 535.2 0 70 184 92 535.2 0 92 185 122 535.2 0 122 186 76 535.2 0 76 187 80 535.2 0 80 188 67 535.2 0 67 189 77 535.2 0 77 190 77 535.2 0 77 191 82 535.2 0 82 192 132 535.2 0 132 193 151 535.2 0 151 194 263 535.2 0 263 195 2496 535.2 0 2496 196 4229 535.2 0 4229 197 2008 535.2 0 2008 198 253 535.2 0 253 199 608 535.2 0 608 200 54 535.2 0 54 201 53 535.2 0 53 202 65 535.2 0 65 203 50 535.2 0 50 204 31 535.2 0 31 205 60 535.2 0 60 206 53 535.2 0 53 207 82 535.2 0 82 208 101 535.2 0 101 209 72 535.2 0 72 210 89 535.2 0 89 211 225 535.2 0 225 212 191 535.2 0 191 213 154 535.2 0 154 214 60 535.2 0 60 215 76 535.2 0 76 216 81 535.2 0 81 217 122 535.2 0 122 218 150 535.2 0 150 219 313 535.2 0 313 220 343 535.2 0 343 221 234 535.2 0 234 222 194 535.2 0 194 223 67 535.2 0 67 224 80 535.2 0 80 225 67 535.2 0 67 226 112 535.2 0 112 227 97 535.2 0 97 228 70 535.2 0 70 229 52 535.2 0 52 230 46 535.2 0 46 231 62 535.2 0 62 232 72 535.2 0 72 233 94 535.2 0 94 234 53 535.2 0 53 235 66 535.2 0 66 236 61 535.2 0 61 237 48 535.2 0 48 238 49 535.2 0 49 239 76 535.2 0 76 240 149 535.2 0 149 241 155 535.2 0 155 242 173 535.2 0 173 243 155 535.2 0 155 244 145 535.2 0 145 245 168 535.2 0 168 246 266 535.2 0 266 247 170 535.2 0 170 248 101 535.2 0 101 249 129 535.2 0 129 250 192 535.2 0 192 251 145 535.2 0 145 252 55 535.2 0 55 253 50 535.2 0 50 254 89 535.2 0 89 255 107 535.2 0 107 256 117 535.2 0 117 257 82 535.2 0 82 258 58 535.2 0 58 259 70 535.2 0 70 260 80 535.2 0 80 261 89 535.2 0 89 262 180 535.2 0 180 263 191 535.2 0 191 264 305 535.2 0 305 265 96 535.2 0 96 266 84 535.2 0 84 267 360 535.2 0 360 268 669 535.2 0 669 269 675 535.2 0 675 270 174 535.2 0 174 271 51 535.2 0 51 272 81 535.2 0 81 273 219 535.2 0 219 274 243 535.2 0 243 275 234 535.2 0 234 276 153 535.2 0 153 277 87 535.2 0 87 278 63 535.2 0 63 279 61 535.2 0 61 280 61 535.2 0 61 281 103 535.2 0 103 282 117 535.2 0 117 283 99 535.2 0 99 284 47 535.2 0 47 285 39 535.2 0 39 286 45 535.2 0 45 287 75 535.2 0 75 288 109 535.2 0 109 289 96 535.2 0 96 290 89 535.2 0 89 291 53 535.2 0 53 292 44 535.2 0 44 293 70 535.2 0 70 294 69 535.2 0 69 295 78 535.2 0 78 296 74 535.2 0 74 297 62 535.2 0 62 298 61 535.2 0 61 299 52 535.2 0 52 300 58 535.2 0 58 301 78 535.2 0 78 Finished in 2.47 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 56,094 Reads with adapters: 16,610 (29.6%) Reads that were too short: 115 (0.2%) Reads written (passing filters): 16,495 (29.4%) Total basepairs processed: 13,009,922 bp Total written (filtered): 4,549,190 bp (35.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16610 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 10 0.0 3 0 0 5 5 16 167 0.0 3 0 133 18 16 17 16035 0.0 3 14638 1100 236 61 18 35 0.0 3 6 19 3 7 19 50 0.0 3 0 0 24 26 20 71 0.0 3 2 0 1 68 24 4 0.0 3 0 0 0 4 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 42 15 0.0 3 0 0 0 15 44 9 0.0 3 0 0 0 9 45 6 0.0 3 0 0 1 5 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 31 0.0 3 0 0 0 31 65 3 0.0 3 0 0 0 3 66 8 0.0 3 0 0 0 8 67 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 52 0.0 3 0 0 6 46 76 3 0.0 3 0 0 0 3 80 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 137 9 0.0 3 0 0 1 8 138 1 0.0 3 0 0 0 1 140 4 0.0 3 0 0 0 4 143 1 0.0 3 0 0 0 1 149 5 0.0 3 0 0 0 5 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 1 162 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 275 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 5 0.0 3 0 0 0 5 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.57 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 16,495 Reads with adapters: 84 (0.5%) Reads written (passing filters): 16,495 (100.0%) Total basepairs processed: 4,549,190 bp Total written (filtered): 4,548,931 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 84 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.9% C: 10.7% G: 27.4% T: 44.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 80 257.7 0 80 4 2 64.4 0 2 5 1 16.1 0 1 6 1 4.0 0 1