This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 55.75 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 64,240 (2.9%) Reads that were too short: 8,097 (0.4%) Reads written (passing filters): 56,143 (2.6%) Total basepairs processed: 659,880,795 bp Total written (filtered): 12,983,653 bp (2.0%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 64240 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 238 535.2 0 238 7 15245 535.2 0 15245 8 16590 535.2 0 16590 9 57 535.2 0 57 10 37 535.2 0 37 11 28 535.2 0 28 12 48 535.2 0 48 13 42 535.2 0 42 14 29 535.2 0 29 15 43 535.2 0 43 16 63 535.2 0 63 17 57 535.2 0 57 18 55 535.2 0 55 19 41 535.2 0 41 20 41 535.2 0 41 21 51 535.2 0 51 22 40 535.2 0 40 23 31 535.2 0 31 24 37 535.2 0 37 25 31 535.2 0 31 26 48 535.2 0 48 27 38 535.2 0 38 28 24 535.2 0 24 29 35 535.2 0 35 30 50 535.2 0 50 31 40 535.2 0 40 32 27 535.2 0 27 33 37 535.2 0 37 34 43 535.2 0 43 35 31 535.2 0 31 36 47 535.2 0 47 37 54 535.2 0 54 38 35 535.2 0 35 39 31 535.2 0 31 40 62 535.2 0 62 41 43 535.2 0 43 42 36 535.2 0 36 43 36 535.2 0 36 44 47 535.2 0 47 45 44 535.2 0 44 46 72 535.2 0 72 47 44 535.2 0 44 48 33 535.2 0 33 49 38 535.2 0 38 50 36 535.2 0 36 51 44 535.2 0 44 52 111 535.2 0 111 53 45 535.2 0 45 54 62 535.2 0 62 55 145 535.2 0 145 56 159 535.2 0 159 57 34 535.2 0 34 58 45 535.2 0 45 59 51 535.2 0 51 60 67 535.2 0 67 61 205 535.2 0 205 62 199 535.2 0 199 63 250 535.2 0 250 64 47 535.2 0 47 65 48 535.2 0 48 66 37 535.2 0 37 67 77 535.2 0 77 68 61 535.2 0 61 69 77 535.2 0 77 70 40 535.2 0 40 71 42 535.2 0 42 72 52 535.2 0 52 73 108 535.2 0 108 74 40 535.2 0 40 75 37 535.2 0 37 76 59 535.2 0 59 77 61 535.2 0 61 78 88 535.2 0 88 79 44 535.2 0 44 80 79 535.2 0 79 81 42 535.2 0 42 82 44 535.2 0 44 83 37 535.2 0 37 84 42 535.2 0 42 85 53 535.2 0 53 86 40 535.2 0 40 87 55 535.2 0 55 88 56 535.2 0 56 89 32 535.2 0 32 90 42 535.2 0 42 91 49 535.2 0 49 92 37 535.2 0 37 93 43 535.2 0 43 94 50 535.2 0 50 95 51 535.2 0 51 96 79 535.2 0 79 97 56 535.2 0 56 98 43 535.2 0 43 99 53 535.2 0 53 100 49 535.2 0 49 101 62 535.2 0 62 102 50 535.2 0 50 103 35 535.2 0 35 104 41 535.2 0 41 105 41 535.2 0 41 106 40 535.2 0 40 107 43 535.2 0 43 108 54 535.2 0 54 109 36 535.2 0 36 110 50 535.2 0 50 111 52 535.2 0 52 112 48 535.2 0 48 113 46 535.2 0 46 114 50 535.2 0 50 115 64 535.2 0 64 116 43 535.2 0 43 117 43 535.2 0 43 118 44 535.2 0 44 119 38 535.2 0 38 120 36 535.2 0 36 121 51 535.2 0 51 122 72 535.2 0 72 123 112 535.2 0 112 124 75 535.2 0 75 125 136 535.2 0 136 126 100 535.2 0 100 127 116 535.2 0 116 128 93 535.2 0 93 129 183 535.2 0 183 130 185 535.2 0 185 131 109 535.2 0 109 132 52 535.2 0 52 133 44 535.2 0 44 134 98 535.2 0 98 135 76 535.2 0 76 136 73 535.2 0 73 137 46 535.2 0 46 138 43 535.2 0 43 139 48 535.2 0 48 140 31 535.2 0 31 141 46 535.2 0 46 142 57 535.2 0 57 143 50 535.2 0 50 144 164 535.2 0 164 145 413 535.2 0 413 146 265 535.2 0 265 147 163 535.2 0 163 148 53 535.2 0 53 149 113 535.2 0 113 150 47 535.2 0 47 151 48 535.2 0 48 152 52 535.2 0 52 153 39 535.2 0 39 154 51 535.2 0 51 155 45 535.2 0 45 156 205 535.2 0 205 157 226 535.2 0 226 158 49 535.2 0 49 159 55 535.2 0 55 160 38 535.2 0 38 161 33 535.2 0 33 162 53 535.2 0 53 163 41 535.2 0 41 164 25 535.2 0 25 165 52 535.2 0 52 166 126 535.2 0 126 167 90 535.2 0 90 168 81 535.2 0 81 169 38 535.2 0 38 170 44 535.2 0 44 171 50 535.2 0 50 172 57 535.2 0 57 173 42 535.2 0 42 174 58 535.2 0 58 175 60 535.2 0 60 176 61 535.2 0 61 177 47 535.2 0 47 178 52 535.2 0 52 179 66 535.2 0 66 180 66 535.2 0 66 181 52 535.2 0 52 182 120 535.2 0 120 183 70 535.2 0 70 184 72 535.2 0 72 185 105 535.2 0 105 186 78 535.2 0 78 187 59 535.2 0 59 188 63 535.2 0 63 189 78 535.2 0 78 190 85 535.2 0 85 191 70 535.2 0 70 192 105 535.2 0 105 193 142 535.2 0 142 194 238 535.2 0 238 195 2828 535.2 0 2828 196 4971 535.2 0 4971 197 2491 535.2 0 2491 198 336 535.2 0 336 199 847 535.2 0 847 200 81 535.2 0 81 201 70 535.2 0 70 202 47 535.2 0 47 203 45 535.2 0 45 204 58 535.2 0 58 205 60 535.2 0 60 206 43 535.2 0 43 207 55 535.2 0 55 208 87 535.2 0 87 209 37 535.2 0 37 210 79 535.2 0 79 211 112 535.2 0 112 212 73 535.2 0 73 213 51 535.2 0 51 214 51 535.2 0 51 215 58 535.2 0 58 216 52 535.2 0 52 217 121 535.2 0 121 218 147 535.2 0 147 219 295 535.2 0 295 220 194 535.2 0 194 221 139 535.2 0 139 222 119 535.2 0 119 223 64 535.2 0 64 224 59 535.2 0 59 225 67 535.2 0 67 226 138 535.2 0 138 227 67 535.2 0 67 228 58 535.2 0 58 229 55 535.2 0 55 230 43 535.2 0 43 231 57 535.2 0 57 232 54 535.2 0 54 233 50 535.2 0 50 234 48 535.2 0 48 235 48 535.2 0 48 236 60 535.2 0 60 237 47 535.2 0 47 238 43 535.2 0 43 239 80 535.2 0 80 240 85 535.2 0 85 241 89 535.2 0 89 242 129 535.2 0 129 243 140 535.2 0 140 244 129 535.2 0 129 245 156 535.2 0 156 246 237 535.2 0 237 247 94 535.2 0 94 248 81 535.2 0 81 249 84 535.2 0 84 250 103 535.2 0 103 251 68 535.2 0 68 252 61 535.2 0 61 253 38 535.2 0 38 254 62 535.2 0 62 255 90 535.2 0 90 256 76 535.2 0 76 257 48 535.2 0 48 258 55 535.2 0 55 259 49 535.2 0 49 260 41 535.2 0 41 261 45 535.2 0 45 262 198 535.2 0 198 263 268 535.2 0 268 264 453 535.2 0 453 265 60 535.2 0 60 266 56 535.2 0 56 267 146 535.2 0 146 268 236 535.2 0 236 269 172 535.2 0 172 270 80 535.2 0 80 271 41 535.2 0 41 272 42 535.2 0 42 273 79 535.2 0 79 274 86 535.2 0 86 275 92 535.2 0 92 276 66 535.2 0 66 277 38 535.2 0 38 278 58 535.2 0 58 279 43 535.2 0 43 280 58 535.2 0 58 281 56 535.2 0 56 282 38 535.2 0 38 283 49 535.2 0 49 284 36 535.2 0 36 285 23 535.2 0 23 286 46 535.2 0 46 287 47 535.2 0 47 288 58 535.2 0 58 289 63 535.2 0 63 290 54 535.2 0 54 291 28 535.2 0 28 292 31 535.2 0 31 293 37 535.2 0 37 294 46 535.2 0 46 295 40 535.2 0 40 296 62 535.2 0 62 297 39 535.2 0 39 298 38 535.2 0 38 299 38 535.2 0 38 300 32 535.2 0 32 301 43 535.2 0 43 Finished in 2.68 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 56,143 Reads with adapters: 17,378 (31.0%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 17,372 (30.9%) Total basepairs processed: 12,983,653 bp Total written (filtered): 4,736,920 bp (36.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17378 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 6 0.0 3 0 0 6 19 203 0.0 3 0 200 3 20 17025 0.0 4 16784 230 8 3 21 54 0.0 4 11 41 1 1 22 3 0.0 4 1 0 2 23 17 0.0 4 0 0 0 16 1 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 56 0.0 4 0 0 0 0 56 140 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 17,372 Reads with adapters: 1,605 (9.2%) Reads written (passing filters): 17,372 (100.0%) Total basepairs processed: 4,736,920 bp Total written (filtered): 4,719,912 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1605 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.9% G: 94.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 271.4 0 8 4 33 67.9 0 33 6 1 4.2 0 1 7 2 1.1 0 2 9 77 0.1 0 1 76 10 190 0.0 1 5 185 11 1289 0.0 1 8 1281 12 5 0.0 1 0 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.