This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.71 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 127,225 (5.8%) Reads that were too short: 20,879 (1.0%) Reads written (passing filters): 106,346 (4.9%) Total basepairs processed: 659,880,795 bp Total written (filtered): 28,432,365 bp (4.3%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 127225 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1076 535.2 0 1076 7 39286 535.2 0 39286 8 936 535.2 0 936 9 42197 535.2 0 42197 10 73 535.2 0 73 11 17 535.2 0 17 12 15 535.2 0 15 13 16 535.2 0 16 14 12 535.2 0 12 15 15 535.2 0 15 16 20 535.2 0 20 17 9 535.2 0 9 18 12 535.2 0 12 19 12 535.2 0 12 20 10 535.2 0 10 21 13 535.2 0 13 22 26 535.2 0 26 23 49 535.2 0 49 24 13 535.2 0 13 25 9 535.2 0 9 26 12 535.2 0 12 27 9 535.2 0 9 28 14 535.2 0 14 29 20 535.2 0 20 30 14 535.2 0 14 31 9 535.2 0 9 32 10 535.2 0 10 33 16 535.2 0 16 34 14 535.2 0 14 35 19 535.2 0 19 36 34 535.2 0 34 37 14 535.2 0 14 38 22 535.2 0 22 39 22 535.2 0 22 40 35 535.2 0 35 41 117 535.2 0 117 42 101 535.2 0 101 43 86 535.2 0 86 44 17 535.2 0 17 45 8 535.2 0 8 46 14 535.2 0 14 47 30 535.2 0 30 48 14 535.2 0 14 49 23 535.2 0 23 50 28 535.2 0 28 51 25 535.2 0 25 52 34 535.2 0 34 53 36 535.2 0 36 54 13 535.2 0 13 55 29 535.2 0 29 56 20 535.2 0 20 57 29 535.2 0 29 58 26 535.2 0 26 59 14 535.2 0 14 60 22 535.2 0 22 61 16 535.2 0 16 62 16 535.2 0 16 63 29 535.2 0 29 64 17 535.2 0 17 65 33 535.2 0 33 66 24 535.2 0 24 67 27 535.2 0 27 68 30 535.2 0 30 69 157 535.2 0 157 70 102 535.2 0 102 71 123 535.2 0 123 72 27 535.2 0 27 73 29 535.2 0 29 74 38 535.2 0 38 75 23 535.2 0 23 76 31 535.2 0 31 77 27 535.2 0 27 78 21 535.2 0 21 79 24 535.2 0 24 80 44 535.2 0 44 81 40 535.2 0 40 82 72 535.2 0 72 83 41 535.2 0 41 84 51 535.2 0 51 85 46 535.2 0 46 86 68 535.2 0 68 87 203 535.2 0 203 88 72 535.2 0 72 89 129 535.2 0 129 90 151 535.2 0 151 91 93 535.2 0 93 92 72 535.2 0 72 93 67 535.2 0 67 94 85 535.2 0 85 95 70 535.2 0 70 96 71 535.2 0 71 97 109 535.2 0 109 98 100 535.2 0 100 99 91 535.2 0 91 100 1013 535.2 0 1013 101 1765 535.2 0 1765 102 3294 535.2 0 3294 103 423 535.2 0 423 104 60 535.2 0 60 105 33 535.2 0 33 106 41 535.2 0 41 107 47 535.2 0 47 108 37 535.2 0 37 109 29 535.2 0 29 110 55 535.2 0 55 111 37 535.2 0 37 112 46 535.2 0 46 113 49 535.2 0 49 114 25 535.2 0 25 115 41 535.2 0 41 116 36 535.2 0 36 117 50 535.2 0 50 118 88 535.2 0 88 119 44 535.2 0 44 120 51 535.2 0 51 121 42 535.2 0 42 122 82 535.2 0 82 123 121 535.2 0 121 124 94 535.2 0 94 125 78 535.2 0 78 126 57 535.2 0 57 127 33 535.2 0 33 128 55 535.2 0 55 129 53 535.2 0 53 130 80 535.2 0 80 131 219 535.2 0 219 132 353 535.2 0 353 133 595 535.2 0 595 134 236 535.2 0 236 135 74 535.2 0 74 136 75 535.2 0 75 137 130 535.2 0 130 138 136 535.2 0 136 139 219 535.2 0 219 140 135 535.2 0 135 141 61 535.2 0 61 142 105 535.2 0 105 143 223 535.2 0 223 144 256 535.2 0 256 145 211 535.2 0 211 146 63 535.2 0 63 147 70 535.2 0 70 148 76 535.2 0 76 149 77 535.2 0 77 150 73 535.2 0 73 151 76 535.2 0 76 152 86 535.2 0 86 153 93 535.2 0 93 154 59 535.2 0 59 155 59 535.2 0 59 156 80 535.2 0 80 157 253 535.2 0 253 158 502 535.2 0 502 159 429 535.2 0 429 160 281 535.2 0 281 161 149 535.2 0 149 162 232 535.2 0 232 163 66 535.2 0 66 164 77 535.2 0 77 165 58 535.2 0 58 166 40 535.2 0 40 167 77 535.2 0 77 168 114 535.2 0 114 169 133 535.2 0 133 170 100 535.2 0 100 171 65 535.2 0 65 172 60 535.2 0 60 173 83 535.2 0 83 174 64 535.2 0 64 175 61 535.2 0 61 176 577 535.2 0 577 177 542 535.2 0 542 178 838 535.2 0 838 179 190 535.2 0 190 180 77 535.2 0 77 181 89 535.2 0 89 182 111 535.2 0 111 183 156 535.2 0 156 184 179 535.2 0 179 185 90 535.2 0 90 186 64 535.2 0 64 187 103 535.2 0 103 188 140 535.2 0 140 189 164 535.2 0 164 190 121 535.2 0 121 191 117 535.2 0 117 192 44 535.2 0 44 193 41 535.2 0 41 194 56 535.2 0 56 195 75 535.2 0 75 196 115 535.2 0 115 197 150 535.2 0 150 198 158 535.2 0 158 199 123 535.2 0 123 200 75 535.2 0 75 201 73 535.2 0 73 202 125 535.2 0 125 203 232 535.2 0 232 204 231 535.2 0 231 205 125 535.2 0 125 206 89 535.2 0 89 207 74 535.2 0 74 208 74 535.2 0 74 209 88 535.2 0 88 210 152 535.2 0 152 211 211 535.2 0 211 212 190 535.2 0 190 213 153 535.2 0 153 214 121 535.2 0 121 215 122 535.2 0 122 216 119 535.2 0 119 217 110 535.2 0 110 218 143 535.2 0 143 219 142 535.2 0 142 220 108 535.2 0 108 221 87 535.2 0 87 222 206 535.2 0 206 223 478 535.2 0 478 224 560 535.2 0 560 225 283 535.2 0 283 226 192 535.2 0 192 227 119 535.2 0 119 228 123 535.2 0 123 229 142 535.2 0 142 230 109 535.2 0 109 231 90 535.2 0 90 232 86 535.2 0 86 233 105 535.2 0 105 234 140 535.2 0 140 235 138 535.2 0 138 236 130 535.2 0 130 237 130 535.2 0 130 238 209 535.2 0 209 239 249 535.2 0 249 240 204 535.2 0 204 241 114 535.2 0 114 242 230 535.2 0 230 243 331 535.2 0 331 244 385 535.2 0 385 245 233 535.2 0 233 246 229 535.2 0 229 247 191 535.2 0 191 248 214 535.2 0 214 249 123 535.2 0 123 250 99 535.2 0 99 251 137 535.2 0 137 252 108 535.2 0 108 253 118 535.2 0 118 254 136 535.2 0 136 255 237 535.2 0 237 256 258 535.2 0 258 257 243 535.2 0 243 258 219 535.2 0 219 259 160 535.2 0 160 260 143 535.2 0 143 261 150 535.2 0 150 262 253 535.2 0 253 263 439 535.2 0 439 264 655 535.2 0 655 265 522 535.2 0 522 266 468 535.2 0 468 267 227 535.2 0 227 268 115 535.2 0 115 269 145 535.2 0 145 270 231 535.2 0 231 271 331 535.2 0 331 272 357 535.2 0 357 273 296 535.2 0 296 274 346 535.2 0 346 275 160 535.2 0 160 276 240 535.2 0 240 277 233 535.2 0 233 278 265 535.2 0 265 279 220 535.2 0 220 280 138 535.2 0 138 281 149 535.2 0 149 282 174 535.2 0 174 283 267 535.2 0 267 284 230 535.2 0 230 285 251 535.2 0 251 286 237 535.2 0 237 287 260 535.2 0 260 288 209 535.2 0 209 289 160 535.2 0 160 290 143 535.2 0 143 291 290 535.2 0 290 292 413 535.2 0 413 293 320 535.2 0 320 294 197 535.2 0 197 295 143 535.2 0 143 296 87 535.2 0 87 297 144 535.2 0 144 298 186 535.2 0 186 299 220 535.2 0 220 300 297 535.2 0 297 301 414 535.2 0 414 Finished in 4.95 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 106,346 Reads with adapters: 43,866 (41.2%) Reads that were too short: 172 (0.2%) Reads written (passing filters): 43,694 (41.1%) Total basepairs processed: 28,432,365 bp Total written (filtered): 11,950,601 bp (42.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 43866 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 36 0.0 3 0 0 26 10 16 491 0.0 3 0 416 40 35 17 42351 0.0 3 38513 3083 589 166 18 107 0.0 3 7 73 11 16 19 140 0.0 3 2 2 80 56 20 95 0.0 3 15 5 0 75 21 2 0.0 3 0 0 0 2 22 37 0.0 3 0 0 0 37 23 6 0.0 3 0 0 2 4 27 1 0.0 3 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 0 3 34 35 0.0 3 0 0 1 34 35 3 0.0 3 1 0 1 1 36 1 0.0 3 0 0 0 1 37 12 0.0 3 0 0 1 11 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 49 16 0.0 3 0 0 0 16 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 58 6 0.0 3 0 0 0 6 59 2 0.0 3 0 0 0 2 60 8 0.0 3 0 0 0 8 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 11 0.0 3 0 0 0 11 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 6 0.0 3 0 0 0 6 70 4 0.0 3 0 0 0 4 71 115 0.0 3 0 0 2 113 72 17 0.0 3 0 0 0 17 73 11 0.0 3 0 0 0 11 74 29 0.0 3 0 0 0 29 75 3 0.0 3 0 0 0 3 76 3 0.0 3 0 0 1 2 79 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 82 4 0.0 3 0 0 0 4 83 5 0.0 3 0 0 0 5 84 12 0.0 3 0 0 0 12 85 3 0.0 3 0 0 0 3 87 4 0.0 3 0 0 1 3 88 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 15 0.0 3 0 0 0 15 92 5 0.0 3 0 0 0 5 93 2 0.0 3 0 0 0 2 94 3 0.0 3 0 0 0 3 95 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 98 4 0.0 3 0 0 0 4 99 2 0.0 3 0 0 0 2 101 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 6 0.0 3 0 0 0 6 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 1 2 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 9 0.0 3 0 0 0 9 123 2 0.0 3 0 0 0 2 124 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 130 4 0.0 3 0 0 0 4 131 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 136 5 0.0 3 0 0 0 5 140 1 0.0 3 0 0 0 1 142 4 0.0 3 0 0 0 4 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 5 0.0 3 0 0 0 5 156 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 164 8 0.0 3 0 0 0 8 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 183 5 0.0 3 0 0 0 5 184 9 0.0 3 0 0 3 6 185 3 0.0 3 0 0 0 3 186 2 0.0 3 0 0 0 2 187 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 1 190 1 0.0 3 0 0 0 1 191 7 0.0 3 0 0 0 7 192 7 0.0 3 0 0 0 7 193 5 0.0 3 0 0 0 5 194 3 0.0 3 0 0 0 3 197 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 206 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 213 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 4 0.0 3 0 0 0 4 281 1 0.0 3 0 0 0 1 282 4 0.0 3 0 0 0 4 283 5 0.0 3 0 0 0 5 290 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.54 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 43,694 Reads with adapters: 2,383 (5.5%) Reads written (passing filters): 43,694 (100.0%) Total basepairs processed: 11,950,601 bp Total written (filtered): 11,943,386 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2383 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.0% C: 14.7% G: 18.9% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2340 682.7 0 2340 4 39 170.7 0 39 9 3 0.2 0 0 3 12 1 0.0 1 0 1