This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.36 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 62,730 (2.9%) Reads that were too short: 33,273 (1.5%) Reads written (passing filters): 29,457 (1.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 6,846,242 bp (1.0%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 62730 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2096 535.2 0 2096 7 251 535.2 0 251 8 5395 535.2 0 5395 9 4635 535.2 0 4635 10 57 535.2 0 57 11 80 535.2 0 80 12 106 535.2 0 106 13 78 535.2 0 78 14 52 535.2 0 52 15 49 535.2 0 49 16 59 535.2 0 59 17 62 535.2 0 62 18 65 535.2 0 65 19 75 535.2 0 75 20 56 535.2 0 56 21 51 535.2 0 51 22 78 535.2 0 78 23 75 535.2 0 75 24 91 535.2 0 91 25 60 535.2 0 60 26 53 535.2 0 53 27 47 535.2 0 47 28 46 535.2 0 46 29 66 535.2 0 66 30 51 535.2 0 51 31 61 535.2 0 61 32 66 535.2 0 66 33 61 535.2 0 61 34 59 535.2 0 59 35 64 535.2 0 64 36 65 535.2 0 65 37 50 535.2 0 50 38 74 535.2 0 74 39 85 535.2 0 85 40 100 535.2 0 100 41 103 535.2 0 103 42 88 535.2 0 88 43 56 535.2 0 56 44 48 535.2 0 48 45 69 535.2 0 69 46 66 535.2 0 66 47 53 535.2 0 53 48 60 535.2 0 60 49 55 535.2 0 55 50 54 535.2 0 54 51 109 535.2 0 109 52 99 535.2 0 99 53 234 535.2 0 234 54 76 535.2 0 76 55 57 535.2 0 57 56 52 535.2 0 52 57 54 535.2 0 54 58 71 535.2 0 71 59 72 535.2 0 72 60 48 535.2 0 48 61 78 535.2 0 78 62 78 535.2 0 78 63 63 535.2 0 63 64 50 535.2 0 50 65 53 535.2 0 53 66 68 535.2 0 68 67 64 535.2 0 64 68 58 535.2 0 58 69 66 535.2 0 66 70 46 535.2 0 46 71 55 535.2 0 55 72 64 535.2 0 64 73 66 535.2 0 66 74 75 535.2 0 75 75 96 535.2 0 96 76 64 535.2 0 64 77 83 535.2 0 83 78 61 535.2 0 61 79 70 535.2 0 70 80 63 535.2 0 63 81 74 535.2 0 74 82 81 535.2 0 81 83 76 535.2 0 76 84 64 535.2 0 64 85 57 535.2 0 57 86 44 535.2 0 44 87 75 535.2 0 75 88 50 535.2 0 50 89 68 535.2 0 68 90 81 535.2 0 81 91 97 535.2 0 97 92 80 535.2 0 80 93 91 535.2 0 91 94 95 535.2 0 95 95 66 535.2 0 66 96 73 535.2 0 73 97 86 535.2 0 86 98 61 535.2 0 61 99 108 535.2 0 108 100 254 535.2 0 254 101 175 535.2 0 175 102 89 535.2 0 89 103 58 535.2 0 58 104 57 535.2 0 57 105 76 535.2 0 76 106 55 535.2 0 55 107 70 535.2 0 70 108 55 535.2 0 55 109 66 535.2 0 66 110 66 535.2 0 66 111 65 535.2 0 65 112 59 535.2 0 59 113 79 535.2 0 79 114 76 535.2 0 76 115 80 535.2 0 80 116 92 535.2 0 92 117 189 535.2 0 189 118 132 535.2 0 132 119 85 535.2 0 85 120 75 535.2 0 75 121 72 535.2 0 72 122 77 535.2 0 77 123 66 535.2 0 66 124 49 535.2 0 49 125 77 535.2 0 77 126 66 535.2 0 66 127 69 535.2 0 69 128 54 535.2 0 54 129 57 535.2 0 57 130 64 535.2 0 64 131 109 535.2 0 109 132 90 535.2 0 90 133 103 535.2 0 103 134 59 535.2 0 59 135 67 535.2 0 67 136 99 535.2 0 99 137 436 535.2 0 436 138 1171 535.2 0 1171 139 803 535.2 0 803 140 740 535.2 0 740 141 58 535.2 0 58 142 64 535.2 0 64 143 85 535.2 0 85 144 110 535.2 0 110 145 87 535.2 0 87 146 75 535.2 0 75 147 67 535.2 0 67 148 62 535.2 0 62 149 67 535.2 0 67 150 74 535.2 0 74 151 69 535.2 0 69 152 67 535.2 0 67 153 49 535.2 0 49 154 54 535.2 0 54 155 62 535.2 0 62 156 87 535.2 0 87 157 80 535.2 0 80 158 73 535.2 0 73 159 72 535.2 0 72 160 69 535.2 0 69 161 73 535.2 0 73 162 59 535.2 0 59 163 61 535.2 0 61 164 59 535.2 0 59 165 81 535.2 0 81 166 64 535.2 0 64 167 55 535.2 0 55 168 70 535.2 0 70 169 72 535.2 0 72 170 73 535.2 0 73 171 104 535.2 0 104 172 102 535.2 0 102 173 91 535.2 0 91 174 56 535.2 0 56 175 68 535.2 0 68 176 63 535.2 0 63 177 60 535.2 0 60 178 87 535.2 0 87 179 81 535.2 0 81 180 70 535.2 0 70 181 109 535.2 0 109 182 80 535.2 0 80 183 65 535.2 0 65 184 81 535.2 0 81 185 90 535.2 0 90 186 79 535.2 0 79 187 83 535.2 0 83 188 75 535.2 0 75 189 86 535.2 0 86 190 59 535.2 0 59 191 81 535.2 0 81 192 72 535.2 0 72 193 77 535.2 0 77 194 65 535.2 0 65 195 72 535.2 0 72 196 60 535.2 0 60 197 83 535.2 0 83 198 86 535.2 0 86 199 75 535.2 0 75 200 67 535.2 0 67 201 60 535.2 0 60 202 64 535.2 0 64 203 89 535.2 0 89 204 93 535.2 0 93 205 84 535.2 0 84 206 77 535.2 0 77 207 81 535.2 0 81 208 75 535.2 0 75 209 73 535.2 0 73 210 108 535.2 0 108 211 106 535.2 0 106 212 85 535.2 0 85 213 82 535.2 0 82 214 126 535.2 0 126 215 125 535.2 0 125 216 131 535.2 0 131 217 87 535.2 0 87 218 97 535.2 0 97 219 65 535.2 0 65 220 92 535.2 0 92 221 69 535.2 0 69 222 80 535.2 0 80 223 87 535.2 0 87 224 120 535.2 0 120 225 103 535.2 0 103 226 70 535.2 0 70 227 182 535.2 0 182 228 97 535.2 0 97 229 98 535.2 0 98 230 88 535.2 0 88 231 80 535.2 0 80 232 64 535.2 0 64 233 90 535.2 0 90 234 65 535.2 0 65 235 57 535.2 0 57 236 54 535.2 0 54 237 82 535.2 0 82 238 142 535.2 0 142 239 166 535.2 0 166 240 173 535.2 0 173 241 68 535.2 0 68 242 72 535.2 0 72 243 98 535.2 0 98 244 70 535.2 0 70 245 80 535.2 0 80 246 76 535.2 0 76 247 77 535.2 0 77 248 82 535.2 0 82 249 71 535.2 0 71 250 64 535.2 0 64 251 105 535.2 0 105 252 101 535.2 0 101 253 97 535.2 0 97 254 95 535.2 0 95 255 79 535.2 0 79 256 126 535.2 0 126 257 175 535.2 0 175 258 265 535.2 0 265 259 214 535.2 0 214 260 176 535.2 0 176 261 97 535.2 0 97 262 87 535.2 0 87 263 111 535.2 0 111 264 128 535.2 0 128 265 99 535.2 0 99 266 134 535.2 0 134 267 96 535.2 0 96 268 95 535.2 0 95 269 86 535.2 0 86 270 144 535.2 0 144 271 196 535.2 0 196 272 279 535.2 0 279 273 1845 535.2 0 1845 274 3243 535.2 0 3243 275 2669 535.2 0 2669 276 225 535.2 0 225 277 434 535.2 0 434 278 322 535.2 0 322 279 191 535.2 0 191 280 122 535.2 0 122 281 97 535.2 0 97 282 127 535.2 0 127 283 260 535.2 0 260 284 342 535.2 0 342 285 736 535.2 0 736 286 1329 535.2 0 1329 287 587 535.2 0 587 288 958 535.2 0 958 289 162 535.2 0 162 290 140 535.2 0 140 291 272 535.2 0 272 292 300 535.2 0 300 293 499 535.2 0 499 294 495 535.2 0 495 295 351 535.2 0 351 296 219 535.2 0 219 297 384 535.2 0 384 298 1432 535.2 0 1432 299 3744 535.2 0 3744 300 2722 535.2 0 2722 301 1616 535.2 0 1616 Finished in 1.30 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 29,457 Reads with adapters: 5,352 (18.2%) Reads that were too short: 53 (0.2%) Reads written (passing filters): 5,299 (18.0%) Total basepairs processed: 6,846,242 bp Total written (filtered): 1,449,095 bp (21.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5352 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 6 0.0 3 0 0 3 3 16 64 0.0 3 0 49 11 4 17 5043 0.0 3 4560 381 83 19 18 25 0.0 3 3 18 2 2 19 47 0.0 3 13 6 16 12 20 22 0.0 3 4 2 2 14 22 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 35 2 0.0 3 1 0 0 1 36 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 55 29 0.0 3 0 0 1 28 58 5 0.0 3 0 0 0 5 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 72 4 0.0 3 0 0 0 4 75 3 0.0 3 0 0 1 2 76 4 0.0 3 0 0 0 4 78 1 0.0 3 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 1 1 136 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 1 2 196 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 220 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 5,299 Reads with adapters: 19 (0.4%) Reads written (passing filters): 5,299 (100.0%) Total basepairs processed: 1,449,095 bp Total written (filtered): 1,449,036 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 19 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.9% C: 10.5% G: 26.3% T: 5.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 17 82.8 0 17 4 2 20.7 0 2