This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.33 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 56,293 (2.6%) Reads that were too short: 32,133 (1.5%) Reads written (passing filters): 24,160 (1.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 5,948,217 bp (0.9%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 56293 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2398 535.2 0 2398 7 168 535.2 0 168 8 5073 535.2 0 5073 9 5475 535.2 0 5475 10 47 535.2 0 47 11 39 535.2 0 39 12 65 535.2 0 65 13 48 535.2 0 48 14 56 535.2 0 56 15 40 535.2 0 40 16 46 535.2 0 46 17 37 535.2 0 37 18 47 535.2 0 47 19 66 535.2 0 66 20 53 535.2 0 53 21 62 535.2 0 62 22 44 535.2 0 44 23 48 535.2 0 48 24 39 535.2 0 39 25 49 535.2 0 49 26 44 535.2 0 44 27 52 535.2 0 52 28 38 535.2 0 38 29 52 535.2 0 52 30 47 535.2 0 47 31 62 535.2 0 62 32 39 535.2 0 39 33 37 535.2 0 37 34 41 535.2 0 41 35 60 535.2 0 60 36 55 535.2 0 55 37 46 535.2 0 46 38 60 535.2 0 60 39 45 535.2 0 45 40 46 535.2 0 46 41 42 535.2 0 42 42 49 535.2 0 49 43 49 535.2 0 49 44 50 535.2 0 50 45 52 535.2 0 52 46 63 535.2 0 63 47 57 535.2 0 57 48 47 535.2 0 47 49 44 535.2 0 44 50 44 535.2 0 44 51 41 535.2 0 41 52 54 535.2 0 54 53 58 535.2 0 58 54 54 535.2 0 54 55 53 535.2 0 53 56 44 535.2 0 44 57 46 535.2 0 46 58 48 535.2 0 48 59 57 535.2 0 57 60 53 535.2 0 53 61 52 535.2 0 52 62 50 535.2 0 50 63 43 535.2 0 43 64 47 535.2 0 47 65 45 535.2 0 45 66 68 535.2 0 68 67 58 535.2 0 58 68 56 535.2 0 56 69 57 535.2 0 57 70 48 535.2 0 48 71 53 535.2 0 53 72 64 535.2 0 64 73 55 535.2 0 55 74 53 535.2 0 53 75 78 535.2 0 78 76 39 535.2 0 39 77 50 535.2 0 50 78 70 535.2 0 70 79 46 535.2 0 46 80 48 535.2 0 48 81 49 535.2 0 49 82 74 535.2 0 74 83 40 535.2 0 40 84 35 535.2 0 35 85 42 535.2 0 42 86 39 535.2 0 39 87 36 535.2 0 36 88 42 535.2 0 42 89 44 535.2 0 44 90 47 535.2 0 47 91 53 535.2 0 53 92 57 535.2 0 57 93 69 535.2 0 69 94 49 535.2 0 49 95 40 535.2 0 40 96 60 535.2 0 60 97 54 535.2 0 54 98 68 535.2 0 68 99 54 535.2 0 54 100 65 535.2 0 65 101 68 535.2 0 68 102 67 535.2 0 67 103 47 535.2 0 47 104 50 535.2 0 50 105 48 535.2 0 48 106 52 535.2 0 52 107 65 535.2 0 65 108 49 535.2 0 49 109 64 535.2 0 64 110 62 535.2 0 62 111 40 535.2 0 40 112 50 535.2 0 50 113 48 535.2 0 48 114 51 535.2 0 51 115 46 535.2 0 46 116 55 535.2 0 55 117 139 535.2 0 139 118 77 535.2 0 77 119 48 535.2 0 48 120 59 535.2 0 59 121 68 535.2 0 68 122 56 535.2 0 56 123 41 535.2 0 41 124 36 535.2 0 36 125 50 535.2 0 50 126 38 535.2 0 38 127 36 535.2 0 36 128 41 535.2 0 41 129 55 535.2 0 55 130 51 535.2 0 51 131 75 535.2 0 75 132 54 535.2 0 54 133 60 535.2 0 60 134 52 535.2 0 52 135 40 535.2 0 40 136 51 535.2 0 51 137 160 535.2 0 160 138 401 535.2 0 401 139 265 535.2 0 265 140 283 535.2 0 283 141 44 535.2 0 44 142 42 535.2 0 42 143 52 535.2 0 52 144 75 535.2 0 75 145 51 535.2 0 51 146 52 535.2 0 52 147 40 535.2 0 40 148 58 535.2 0 58 149 57 535.2 0 57 150 54 535.2 0 54 151 50 535.2 0 50 152 43 535.2 0 43 153 56 535.2 0 56 154 48 535.2 0 48 155 46 535.2 0 46 156 50 535.2 0 50 157 56 535.2 0 56 158 47 535.2 0 47 159 42 535.2 0 42 160 48 535.2 0 48 161 61 535.2 0 61 162 66 535.2 0 66 163 46 535.2 0 46 164 49 535.2 0 49 165 55 535.2 0 55 166 55 535.2 0 55 167 45 535.2 0 45 168 44 535.2 0 44 169 50 535.2 0 50 170 42 535.2 0 42 171 72 535.2 0 72 172 67 535.2 0 67 173 75 535.2 0 75 174 59 535.2 0 59 175 44 535.2 0 44 176 60 535.2 0 60 177 47 535.2 0 47 178 82 535.2 0 82 179 81 535.2 0 81 180 63 535.2 0 63 181 67 535.2 0 67 182 44 535.2 0 44 183 61 535.2 0 61 184 56 535.2 0 56 185 62 535.2 0 62 186 58 535.2 0 58 187 62 535.2 0 62 188 54 535.2 0 54 189 62 535.2 0 62 190 43 535.2 0 43 191 41 535.2 0 41 192 69 535.2 0 69 193 51 535.2 0 51 194 54 535.2 0 54 195 57 535.2 0 57 196 54 535.2 0 54 197 50 535.2 0 50 198 59 535.2 0 59 199 58 535.2 0 58 200 52 535.2 0 52 201 48 535.2 0 48 202 71 535.2 0 71 203 44 535.2 0 44 204 71 535.2 0 71 205 50 535.2 0 50 206 47 535.2 0 47 207 53 535.2 0 53 208 69 535.2 0 69 209 67 535.2 0 67 210 59 535.2 0 59 211 51 535.2 0 51 212 75 535.2 0 75 213 54 535.2 0 54 214 79 535.2 0 79 215 52 535.2 0 52 216 69 535.2 0 69 217 69 535.2 0 69 218 66 535.2 0 66 219 61 535.2 0 61 220 61 535.2 0 61 221 47 535.2 0 47 222 64 535.2 0 64 223 63 535.2 0 63 224 102 535.2 0 102 225 77 535.2 0 77 226 78 535.2 0 78 227 212 535.2 0 212 228 72 535.2 0 72 229 42 535.2 0 42 230 45 535.2 0 45 231 58 535.2 0 58 232 31 535.2 0 31 233 76 535.2 0 76 234 57 535.2 0 57 235 67 535.2 0 67 236 55 535.2 0 55 237 49 535.2 0 49 238 71 535.2 0 71 239 104 535.2 0 104 240 103 535.2 0 103 241 59 535.2 0 59 242 76 535.2 0 76 243 56 535.2 0 56 244 55 535.2 0 55 245 60 535.2 0 60 246 52 535.2 0 52 247 52 535.2 0 52 248 83 535.2 0 83 249 62 535.2 0 62 250 58 535.2 0 58 251 81 535.2 0 81 252 71 535.2 0 71 253 54 535.2 0 54 254 44 535.2 0 44 255 51 535.2 0 51 256 62 535.2 0 62 257 73 535.2 0 73 258 93 535.2 0 93 259 100 535.2 0 100 260 124 535.2 0 124 261 73 535.2 0 73 262 64 535.2 0 64 263 85 535.2 0 85 264 65 535.2 0 65 265 78 535.2 0 78 266 91 535.2 0 91 267 97 535.2 0 97 268 102 535.2 0 102 269 96 535.2 0 96 270 140 535.2 0 140 271 146 535.2 0 146 272 207 535.2 0 207 273 2132 535.2 0 2132 274 3789 535.2 0 3789 275 3203 535.2 0 3203 276 96 535.2 0 96 277 113 535.2 0 113 278 131 535.2 0 131 279 98 535.2 0 98 280 84 535.2 0 84 281 81 535.2 0 81 282 82 535.2 0 82 283 136 535.2 0 136 284 218 535.2 0 218 285 509 535.2 0 509 286 1068 535.2 0 1068 287 305 535.2 0 305 288 364 535.2 0 364 289 85 535.2 0 85 290 106 535.2 0 106 291 121 535.2 0 121 292 190 535.2 0 190 293 655 535.2 0 655 294 783 535.2 0 783 295 442 535.2 0 442 296 152 535.2 0 152 297 355 535.2 0 355 298 1231 535.2 0 1231 299 3970 535.2 0 3970 300 3572 535.2 0 3572 301 2811 535.2 0 2811 Finished in 1.21 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 24,160 Reads with adapters: 7,952 (32.9%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 7,945 (32.9%) Total basepairs processed: 5,948,217 bp Total written (filtered): 2,162,888 bp (36.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7952 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 84 0.0 3 0 82 2 20 5548 0.0 4 5436 109 3 21 29 0.0 4 3 26 22 2253 0.0 4 2213 38 2 23 15 0.0 4 4 4 0 5 2 24 1 0.0 4 0 1 25 1 0.0 4 0 0 0 1 26 1 0.0 4 1 53 1 0.0 4 0 0 0 0 1 55 1 0.0 4 1 125 1 0.0 4 0 0 1 127 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 169 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 268 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 7,945 Reads with adapters: 1,235 (15.5%) Reads written (passing filters): 7,945 (100.0%) Total basepairs processed: 2,162,888 bp Total written (filtered): 2,148,525 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1235 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 3.0% G: 94.7% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 124.1 0 2 4 3 31.0 0 3 7 1 0.5 0 1 9 515 0.0 0 18 497 10 370 0.0 1 9 361 11 326 0.0 1 0 326 12 4 0.0 1 0 4 13 2 0.0 1 0 2 90 1 0.0 1 1 150 2 0.0 1 0 2 169 1 0.0 1 1 223 8 0.0 1 8 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.