This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.80 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 81,499 (3.7%) Reads that were too short: 24,761 (1.1%) Reads written (passing filters): 56,738 (2.6%) Total basepairs processed: 659,880,795 bp Total written (filtered): 12,848,147 bp (1.9%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 81499 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 913 535.2 0 913 7 12074 535.2 0 12074 8 11369 535.2 0 11369 9 32 535.2 0 32 10 42 535.2 0 42 11 48 535.2 0 48 12 42 535.2 0 42 13 23 535.2 0 23 14 37 535.2 0 37 15 25 535.2 0 25 16 21 535.2 0 21 17 31 535.2 0 31 18 20 535.2 0 20 19 25 535.2 0 25 20 26 535.2 0 26 21 29 535.2 0 29 22 27 535.2 0 27 23 35 535.2 0 35 24 22 535.2 0 22 25 32 535.2 0 32 26 31 535.2 0 31 27 43 535.2 0 43 28 35 535.2 0 35 29 33 535.2 0 33 30 80 535.2 0 80 31 100 535.2 0 100 32 39 535.2 0 39 33 31 535.2 0 31 34 34 535.2 0 34 35 34 535.2 0 34 36 30 535.2 0 30 37 56 535.2 0 56 38 30 535.2 0 30 39 27 535.2 0 27 40 60 535.2 0 60 41 33 535.2 0 33 42 36 535.2 0 36 43 51 535.2 0 51 44 51 535.2 0 51 45 49 535.2 0 49 46 47 535.2 0 47 47 54 535.2 0 54 48 66 535.2 0 66 49 114 535.2 0 114 50 1271 535.2 0 1271 51 1251 535.2 0 1251 52 1792 535.2 0 1792 53 39 535.2 0 39 54 47 535.2 0 47 55 28 535.2 0 28 56 24 535.2 0 24 57 30 535.2 0 30 58 45 535.2 0 45 59 35 535.2 0 35 60 43 535.2 0 43 61 52 535.2 0 52 62 45 535.2 0 45 63 46 535.2 0 46 64 83 535.2 0 83 65 61 535.2 0 61 66 59 535.2 0 59 67 52 535.2 0 52 68 57 535.2 0 57 69 33 535.2 0 33 70 37 535.2 0 37 71 51 535.2 0 51 72 62 535.2 0 62 73 101 535.2 0 101 74 634 535.2 0 634 75 887 535.2 0 887 76 1960 535.2 0 1960 77 105 535.2 0 105 78 338 535.2 0 338 79 111 535.2 0 111 80 151 535.2 0 151 81 45 535.2 0 45 82 47 535.2 0 47 83 34 535.2 0 34 84 42 535.2 0 42 85 43 535.2 0 43 86 35 535.2 0 35 87 32 535.2 0 32 88 38 535.2 0 38 89 29 535.2 0 29 90 40 535.2 0 40 91 36 535.2 0 36 92 43 535.2 0 43 93 48 535.2 0 48 94 33 535.2 0 33 95 31 535.2 0 31 96 24 535.2 0 24 97 37 535.2 0 37 98 51 535.2 0 51 99 33 535.2 0 33 100 37 535.2 0 37 101 41 535.2 0 41 102 23 535.2 0 23 103 32 535.2 0 32 104 29 535.2 0 29 105 35 535.2 0 35 106 32 535.2 0 32 107 34 535.2 0 34 108 21 535.2 0 21 109 31 535.2 0 31 110 28 535.2 0 28 111 53 535.2 0 53 112 42 535.2 0 42 113 45 535.2 0 45 114 89 535.2 0 89 115 181 535.2 0 181 116 217 535.2 0 217 117 200 535.2 0 200 118 126 535.2 0 126 119 125 535.2 0 125 120 110 535.2 0 110 121 62 535.2 0 62 122 83 535.2 0 83 123 102 535.2 0 102 124 126 535.2 0 126 125 777 535.2 0 777 126 1246 535.2 0 1246 127 1293 535.2 0 1293 128 74 535.2 0 74 129 96 535.2 0 96 130 286 535.2 0 286 131 254 535.2 0 254 132 167 535.2 0 167 133 57 535.2 0 57 134 64 535.2 0 64 135 85 535.2 0 85 136 57 535.2 0 57 137 80 535.2 0 80 138 81 535.2 0 81 139 39 535.2 0 39 140 47 535.2 0 47 141 52 535.2 0 52 142 49 535.2 0 49 143 39 535.2 0 39 144 49 535.2 0 49 145 37 535.2 0 37 146 55 535.2 0 55 147 38 535.2 0 38 148 42 535.2 0 42 149 40 535.2 0 40 150 39 535.2 0 39 151 76 535.2 0 76 152 44 535.2 0 44 153 63 535.2 0 63 154 78 535.2 0 78 155 60 535.2 0 60 156 33 535.2 0 33 157 75 535.2 0 75 158 88 535.2 0 88 159 87 535.2 0 87 160 70 535.2 0 70 161 70 535.2 0 70 162 93 535.2 0 93 163 73 535.2 0 73 164 691 535.2 0 691 165 1560 535.2 0 1560 166 1824 535.2 0 1824 167 48 535.2 0 48 168 74 535.2 0 74 169 50 535.2 0 50 170 80 535.2 0 80 171 68 535.2 0 68 172 46 535.2 0 46 173 43 535.2 0 43 174 55 535.2 0 55 175 39 535.2 0 39 176 58 535.2 0 58 177 56 535.2 0 56 178 60 535.2 0 60 179 107 535.2 0 107 180 110 535.2 0 110 181 169 535.2 0 169 182 62 535.2 0 62 183 95 535.2 0 95 184 66 535.2 0 66 185 72 535.2 0 72 186 57 535.2 0 57 187 60 535.2 0 60 188 65 535.2 0 65 189 126 535.2 0 126 190 152 535.2 0 152 191 285 535.2 0 285 192 326 535.2 0 326 193 323 535.2 0 323 194 225 535.2 0 225 195 215 535.2 0 215 196 1250 535.2 0 1250 197 1581 535.2 0 1581 198 1594 535.2 0 1594 199 461 535.2 0 461 200 204 535.2 0 204 201 136 535.2 0 136 202 148 535.2 0 148 203 94 535.2 0 94 204 125 535.2 0 125 205 80 535.2 0 80 206 127 535.2 0 127 207 74 535.2 0 74 208 81 535.2 0 81 209 86 535.2 0 86 210 109 535.2 0 109 211 160 535.2 0 160 212 121 535.2 0 121 213 140 535.2 0 140 214 128 535.2 0 128 215 200 535.2 0 200 216 1133 535.2 0 1133 217 2114 535.2 0 2114 218 1412 535.2 0 1412 219 314 535.2 0 314 220 174 535.2 0 174 221 161 535.2 0 161 222 221 535.2 0 221 223 159 535.2 0 159 224 110 535.2 0 110 225 97 535.2 0 97 226 89 535.2 0 89 227 96 535.2 0 96 228 69 535.2 0 69 229 79 535.2 0 79 230 102 535.2 0 102 231 76 535.2 0 76 232 81 535.2 0 81 233 98 535.2 0 98 234 97 535.2 0 97 235 75 535.2 0 75 236 83 535.2 0 83 237 79 535.2 0 79 238 120 535.2 0 120 239 143 535.2 0 143 240 163 535.2 0 163 241 237 535.2 0 237 242 150 535.2 0 150 243 109 535.2 0 109 244 112 535.2 0 112 245 139 535.2 0 139 246 143 535.2 0 143 247 903 535.2 0 903 248 2170 535.2 0 2170 249 2227 535.2 0 2227 250 705 535.2 0 705 251 323 535.2 0 323 252 221 535.2 0 221 253 172 535.2 0 172 254 153 535.2 0 153 255 137 535.2 0 137 256 176 535.2 0 176 257 88 535.2 0 88 258 132 535.2 0 132 259 104 535.2 0 104 260 73 535.2 0 73 261 81 535.2 0 81 262 117 535.2 0 117 263 86 535.2 0 86 264 86 535.2 0 86 265 88 535.2 0 88 266 107 535.2 0 107 267 70 535.2 0 70 268 54 535.2 0 54 269 85 535.2 0 85 270 148 535.2 0 148 271 157 535.2 0 157 272 88 535.2 0 88 273 86 535.2 0 86 274 74 535.2 0 74 275 79 535.2 0 79 276 124 535.2 0 124 277 171 535.2 0 171 278 161 535.2 0 161 279 107 535.2 0 107 280 73 535.2 0 73 281 60 535.2 0 60 282 56 535.2 0 56 283 74 535.2 0 74 284 83 535.2 0 83 285 102 535.2 0 102 286 105 535.2 0 105 287 118 535.2 0 118 288 79 535.2 0 79 289 269 535.2 0 269 290 580 535.2 0 580 291 855 535.2 0 855 292 529 535.2 0 529 293 152 535.2 0 152 294 109 535.2 0 109 295 90 535.2 0 90 296 111 535.2 0 111 297 354 535.2 0 354 298 469 535.2 0 469 299 532 535.2 0 532 300 304 535.2 0 304 301 196 535.2 0 196 Finished in 2.46 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 56,738 Reads with adapters: 13,169 (23.2%) Reads that were too short: 215 (0.4%) Reads written (passing filters): 12,954 (22.8%) Total basepairs processed: 12,848,147 bp Total written (filtered): 3,521,663 bp (27.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13169 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 7 0.0 3 0 0 4 3 16 121 0.0 3 0 104 11 6 17 12255 0.0 3 11185 876 159 35 18 66 0.0 3 1 28 3 34 19 47 0.0 3 2 0 24 21 20 18 0.0 3 1 0 0 17 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 10 0.0 3 0 0 0 10 27 105 0.0 3 0 0 0 105 28 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 30 16 0.0 3 0 0 2 14 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 2 0.0 3 0 0 0 2 43 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 45 4 0.0 3 0 0 0 4 46 85 0.0 3 0 0 1 84 47 1 0.0 3 0 0 0 1 48 4 0.0 3 0 0 0 4 49 28 0.0 3 0 0 2 26 50 4 0.0 3 0 0 0 4 51 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 0 3 53 9 0.0 3 0 0 0 9 54 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 61 2 0.0 3 0 0 0 2 62 7 0.0 3 0 0 0 7 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 1 70 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 73 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 3 0.0 3 0 0 0 3 78 4 0.0 3 0 0 0 4 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 2 0.0 3 0 0 0 2 87 9 0.0 3 0 0 0 9 88 8 0.0 3 0 0 0 8 89 5 0.0 3 0 0 0 5 90 8 0.0 3 0 0 0 8 91 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 1 4 93 4 0.0 3 0 0 1 3 94 3 0.0 3 0 0 0 3 95 6 0.0 3 0 0 0 6 96 1 0.0 3 0 0 0 1 97 43 0.0 3 0 0 1 42 98 2 0.0 3 0 0 0 2 99 4 0.0 3 0 0 0 4 100 10 0.0 3 0 0 0 10 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 0 4 105 8 0.0 3 0 0 0 8 106 3 0.0 3 0 0 0 3 107 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 7 0.0 3 0 0 0 7 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 116 4 0.0 3 0 0 0 4 117 23 0.0 3 0 0 0 23 118 13 0.0 3 0 0 0 13 119 5 0.0 3 0 0 0 5 120 4 0.0 3 0 0 1 3 121 4 0.0 3 0 0 0 4 122 2 0.0 3 0 0 0 2 123 4 0.0 3 0 0 0 4 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 3 0.0 3 0 0 0 3 131 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 5 0.0 3 0 0 0 5 142 1 0.0 3 0 0 0 1 143 5 0.0 3 0 0 0 5 144 2 0.0 3 0 0 1 1 148 2 0.0 3 0 0 0 2 149 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 158 7 0.0 3 0 0 1 6 159 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 1 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 166 2 0.0 3 0 0 1 1 167 3 0.0 3 0 0 0 3 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 3 0.0 3 0 0 0 3 177 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 206 1 0.0 3 0 0 1 208 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 9 0.0 3 0 0 0 9 219 4 0.0 3 0 0 0 4 221 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 1 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 12,954 Reads with adapters: 106 (0.8%) Reads written (passing filters): 12,954 (100.0%) Total basepairs processed: 3,521,663 bp Total written (filtered): 3,521,337 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 106 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.8% C: 17.9% G: 38.7% T: 7.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 100 202.4 0 100 4 5 50.6 0 5 6 1 3.2 0 1