This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.29 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 60,081 (2.7%) Reads that were too short: 11,504 (0.5%) Reads written (passing filters): 48,577 (2.2%) Total basepairs processed: 659,880,795 bp Total written (filtered): 11,812,715 bp (1.8%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 60081 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 943 535.2 0 943 7 11703 535.2 0 11703 8 13484 535.2 0 13484 9 48 535.2 0 48 10 50 535.2 0 50 11 72 535.2 0 72 12 40 535.2 0 40 13 48 535.2 0 48 14 66 535.2 0 66 15 42 535.2 0 42 16 27 535.2 0 27 17 37 535.2 0 37 18 32 535.2 0 32 19 39 535.2 0 39 20 36 535.2 0 36 21 37 535.2 0 37 22 46 535.2 0 46 23 27 535.2 0 27 24 42 535.2 0 42 25 44 535.2 0 44 26 46 535.2 0 46 27 32 535.2 0 32 28 40 535.2 0 40 29 33 535.2 0 33 30 86 535.2 0 86 31 98 535.2 0 98 32 27 535.2 0 27 33 50 535.2 0 50 34 50 535.2 0 50 35 50 535.2 0 50 36 41 535.2 0 41 37 54 535.2 0 54 38 45 535.2 0 45 39 47 535.2 0 47 40 59 535.2 0 59 41 41 535.2 0 41 42 45 535.2 0 45 43 61 535.2 0 61 44 51 535.2 0 51 45 47 535.2 0 47 46 41 535.2 0 41 47 59 535.2 0 59 48 66 535.2 0 66 49 69 535.2 0 69 50 1125 535.2 0 1125 51 1216 535.2 0 1216 52 1796 535.2 0 1796 53 27 535.2 0 27 54 60 535.2 0 60 55 36 535.2 0 36 56 32 535.2 0 32 57 42 535.2 0 42 58 43 535.2 0 43 59 38 535.2 0 38 60 41 535.2 0 41 61 42 535.2 0 42 62 20 535.2 0 20 63 40 535.2 0 40 64 47 535.2 0 47 65 70 535.2 0 70 66 47 535.2 0 47 67 45 535.2 0 45 68 48 535.2 0 48 69 34 535.2 0 34 70 26 535.2 0 26 71 33 535.2 0 33 72 35 535.2 0 35 73 45 535.2 0 45 74 147 535.2 0 147 75 269 535.2 0 269 76 365 535.2 0 365 77 38 535.2 0 38 78 80 535.2 0 80 79 31 535.2 0 31 80 58 535.2 0 58 81 44 535.2 0 44 82 44 535.2 0 44 83 40 535.2 0 40 84 43 535.2 0 43 85 47 535.2 0 47 86 41 535.2 0 41 87 25 535.2 0 25 88 33 535.2 0 33 89 26 535.2 0 26 90 40 535.2 0 40 91 35 535.2 0 35 92 37 535.2 0 37 93 49 535.2 0 49 94 33 535.2 0 33 95 31 535.2 0 31 96 42 535.2 0 42 97 45 535.2 0 45 98 70 535.2 0 70 99 52 535.2 0 52 100 48 535.2 0 48 101 56 535.2 0 56 102 33 535.2 0 33 103 62 535.2 0 62 104 51 535.2 0 51 105 47 535.2 0 47 106 38 535.2 0 38 107 42 535.2 0 42 108 29 535.2 0 29 109 40 535.2 0 40 110 42 535.2 0 42 111 53 535.2 0 53 112 63 535.2 0 63 113 38 535.2 0 38 114 80 535.2 0 80 115 119 535.2 0 119 116 163 535.2 0 163 117 192 535.2 0 192 118 91 535.2 0 91 119 77 535.2 0 77 120 79 535.2 0 79 121 66 535.2 0 66 122 62 535.2 0 62 123 68 535.2 0 68 124 90 535.2 0 90 125 521 535.2 0 521 126 850 535.2 0 850 127 899 535.2 0 899 128 44 535.2 0 44 129 55 535.2 0 55 130 150 535.2 0 150 131 76 535.2 0 76 132 118 535.2 0 118 133 44 535.2 0 44 134 37 535.2 0 37 135 77 535.2 0 77 136 42 535.2 0 42 137 71 535.2 0 71 138 56 535.2 0 56 139 39 535.2 0 39 140 39 535.2 0 39 141 57 535.2 0 57 142 43 535.2 0 43 143 26 535.2 0 26 144 48 535.2 0 48 145 39 535.2 0 39 146 24 535.2 0 24 147 54 535.2 0 54 148 28 535.2 0 28 149 46 535.2 0 46 150 51 535.2 0 51 151 61 535.2 0 61 152 49 535.2 0 49 153 57 535.2 0 57 154 62 535.2 0 62 155 51 535.2 0 51 156 37 535.2 0 37 157 61 535.2 0 61 158 90 535.2 0 90 159 72 535.2 0 72 160 78 535.2 0 78 161 51 535.2 0 51 162 71 535.2 0 71 163 70 535.2 0 70 164 632 535.2 0 632 165 1556 535.2 0 1556 166 1703 535.2 0 1703 167 49 535.2 0 49 168 107 535.2 0 107 169 26 535.2 0 26 170 90 535.2 0 90 171 32 535.2 0 32 172 32 535.2 0 32 173 39 535.2 0 39 174 41 535.2 0 41 175 42 535.2 0 42 176 33 535.2 0 33 177 48 535.2 0 48 178 48 535.2 0 48 179 55 535.2 0 55 180 48 535.2 0 48 181 61 535.2 0 61 182 46 535.2 0 46 183 64 535.2 0 64 184 53 535.2 0 53 185 39 535.2 0 39 186 51 535.2 0 51 187 42 535.2 0 42 188 40 535.2 0 40 189 58 535.2 0 58 190 69 535.2 0 69 191 87 535.2 0 87 192 104 535.2 0 104 193 173 535.2 0 173 194 111 535.2 0 111 195 84 535.2 0 84 196 481 535.2 0 481 197 586 535.2 0 586 198 587 535.2 0 587 199 202 535.2 0 202 200 65 535.2 0 65 201 66 535.2 0 66 202 66 535.2 0 66 203 65 535.2 0 65 204 125 535.2 0 125 205 57 535.2 0 57 206 134 535.2 0 134 207 66 535.2 0 66 208 65 535.2 0 65 209 70 535.2 0 70 210 94 535.2 0 94 211 107 535.2 0 107 212 58 535.2 0 58 213 100 535.2 0 100 214 58 535.2 0 58 215 86 535.2 0 86 216 429 535.2 0 429 217 776 535.2 0 776 218 560 535.2 0 560 219 160 535.2 0 160 220 60 535.2 0 60 221 62 535.2 0 62 222 82 535.2 0 82 223 93 535.2 0 93 224 55 535.2 0 55 225 68 535.2 0 68 226 105 535.2 0 105 227 128 535.2 0 128 228 60 535.2 0 60 229 56 535.2 0 56 230 75 535.2 0 75 231 57 535.2 0 57 232 55 535.2 0 55 233 57 535.2 0 57 234 51 535.2 0 51 235 63 535.2 0 63 236 58 535.2 0 58 237 42 535.2 0 42 238 84 535.2 0 84 239 91 535.2 0 91 240 78 535.2 0 78 241 108 535.2 0 108 242 68 535.2 0 68 243 59 535.2 0 59 244 51 535.2 0 51 245 61 535.2 0 61 246 89 535.2 0 89 247 327 535.2 0 327 248 749 535.2 0 749 249 982 535.2 0 982 250 376 535.2 0 376 251 95 535.2 0 95 252 75 535.2 0 75 253 64 535.2 0 64 254 65 535.2 0 65 255 70 535.2 0 70 256 62 535.2 0 62 257 53 535.2 0 53 258 68 535.2 0 68 259 80 535.2 0 80 260 62 535.2 0 62 261 43 535.2 0 43 262 58 535.2 0 58 263 49 535.2 0 49 264 44 535.2 0 44 265 46 535.2 0 46 266 53 535.2 0 53 267 56 535.2 0 56 268 65 535.2 0 65 269 50 535.2 0 50 270 77 535.2 0 77 271 109 535.2 0 109 272 55 535.2 0 55 273 50 535.2 0 50 274 52 535.2 0 52 275 62 535.2 0 62 276 78 535.2 0 78 277 65 535.2 0 65 278 68 535.2 0 68 279 53 535.2 0 53 280 66 535.2 0 66 281 70 535.2 0 70 282 54 535.2 0 54 283 48 535.2 0 48 284 60 535.2 0 60 285 48 535.2 0 48 286 58 535.2 0 58 287 62 535.2 0 62 288 46 535.2 0 46 289 81 535.2 0 81 290 173 535.2 0 173 291 322 535.2 0 322 292 192 535.2 0 192 293 117 535.2 0 117 294 61 535.2 0 61 295 49 535.2 0 49 296 66 535.2 0 66 297 145 535.2 0 145 298 132 535.2 0 132 299 148 535.2 0 148 300 120 535.2 0 120 301 133 535.2 0 133 Finished in 2.38 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 48,577 Reads with adapters: 13,884 (28.6%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 13,868 (28.5%) Total basepairs processed: 11,812,715 bp Total written (filtered): 3,785,590 bp (32.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13884 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 0 2 18 5 0.0 3 0 0 3 1 1 19 149 0.0 3 0 148 1 20 13648 0.0 4 13458 182 6 0 2 21 40 0.0 4 6 33 0 1 22 1 0.0 4 0 0 1 23 12 0.0 4 0 0 0 10 2 24 2 0.0 4 1 0 0 0 1 34 1 0.0 4 1 53 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 1 84 5 0.0 4 0 0 0 0 5 99 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 185 5 0.0 4 0 0 0 0 5 187 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 13,868 Reads with adapters: 1,292 (9.3%) Reads written (passing filters): 13,868 (100.0%) Total basepairs processed: 3,785,590 bp Total written (filtered): 3,771,398 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1292 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 2.4% G: 94.9% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 216.7 0 4 4 2 54.2 0 2 6 1 3.4 0 1 9 6 0.1 0 0 6 10 166 0.0 1 3 163 11 1105 0.0 1 5 1100 12 5 0.0 1 1 4 13 1 0.0 1 0 1 15 1 0.0 1 0 1 209 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.