This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.75 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 436,755 (19.9%) Reads that were too short: 19,729 (0.9%) Reads written (passing filters): 417,026 (19.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 92,157,182 bp (14.0%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 436755 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 312 535.2 0 312 7 658 535.2 0 658 8 8909 535.2 0 8909 9 6876 535.2 0 6876 10 94 535.2 0 94 11 101 535.2 0 101 12 119 535.2 0 119 13 106 535.2 0 106 14 96 535.2 0 96 15 121 535.2 0 121 16 78 535.2 0 78 17 94 535.2 0 94 18 132 535.2 0 132 19 148 535.2 0 148 20 137 535.2 0 137 21 101 535.2 0 101 22 166 535.2 0 166 23 314 535.2 0 314 24 275 535.2 0 275 25 126 535.2 0 126 26 118 535.2 0 118 27 149 535.2 0 149 28 102 535.2 0 102 29 123 535.2 0 123 30 160 535.2 0 160 31 119 535.2 0 119 32 147 535.2 0 147 33 132 535.2 0 132 34 152 535.2 0 152 35 144 535.2 0 144 36 269 535.2 0 269 37 218 535.2 0 218 38 493 535.2 0 493 39 246 535.2 0 246 40 195 535.2 0 195 41 117 535.2 0 117 42 244 535.2 0 244 43 158 535.2 0 158 44 208 535.2 0 208 45 337 535.2 0 337 46 313 535.2 0 313 47 430 535.2 0 430 48 767 535.2 0 767 49 1734 535.2 0 1734 50 454 535.2 0 454 51 625 535.2 0 625 52 1088 535.2 0 1088 53 739 535.2 0 739 54 675 535.2 0 675 55 1052 535.2 0 1052 56 1183 535.2 0 1183 57 1434 535.2 0 1434 58 1718 535.2 0 1718 59 2766 535.2 0 2766 60 2681 535.2 0 2681 61 3967 535.2 0 3967 62 63867 535.2 0 63867 63 113385 535.2 0 113385 64 97943 535.2 0 97943 65 686 535.2 0 686 66 137 535.2 0 137 67 95 535.2 0 95 68 103 535.2 0 103 69 122 535.2 0 122 70 164 535.2 0 164 71 717 535.2 0 717 72 835 535.2 0 835 73 848 535.2 0 848 74 237 535.2 0 237 75 211 535.2 0 211 76 2192 535.2 0 2192 77 1401 535.2 0 1401 78 1073 535.2 0 1073 79 177 535.2 0 177 80 90 535.2 0 90 81 92 535.2 0 92 82 104 535.2 0 104 83 108 535.2 0 108 84 93 535.2 0 93 85 93 535.2 0 93 86 99 535.2 0 99 87 106 535.2 0 106 88 85 535.2 0 85 89 69 535.2 0 69 90 96 535.2 0 96 91 114 535.2 0 114 92 96 535.2 0 96 93 164 535.2 0 164 94 122 535.2 0 122 95 106 535.2 0 106 96 58 535.2 0 58 97 76 535.2 0 76 98 87 535.2 0 87 99 110 535.2 0 110 100 118 535.2 0 118 101 98 535.2 0 98 102 139 535.2 0 139 103 181 535.2 0 181 104 184 535.2 0 184 105 171 535.2 0 171 106 229 535.2 0 229 107 1150 535.2 0 1150 108 788 535.2 0 788 109 1500 535.2 0 1500 110 152 535.2 0 152 111 226 535.2 0 226 112 214 535.2 0 214 113 89 535.2 0 89 114 91 535.2 0 91 115 82 535.2 0 82 116 106 535.2 0 106 117 114 535.2 0 114 118 109 535.2 0 109 119 87 535.2 0 87 120 87 535.2 0 87 121 97 535.2 0 97 122 69 535.2 0 69 123 67 535.2 0 67 124 93 535.2 0 93 125 127 535.2 0 127 126 167 535.2 0 167 127 210 535.2 0 210 128 225 535.2 0 225 129 144 535.2 0 144 130 126 535.2 0 126 131 143 535.2 0 143 132 168 535.2 0 168 133 146 535.2 0 146 134 146 535.2 0 146 135 110 535.2 0 110 136 127 535.2 0 127 137 190 535.2 0 190 138 127 535.2 0 127 139 170 535.2 0 170 140 253 535.2 0 253 141 237 535.2 0 237 142 497 535.2 0 497 143 720 535.2 0 720 144 7672 535.2 0 7672 145 5071 535.2 0 5071 146 5618 535.2 0 5618 147 178 535.2 0 178 148 129 535.2 0 129 149 167 535.2 0 167 150 161 535.2 0 161 151 166 535.2 0 166 152 187 535.2 0 187 153 176 535.2 0 176 154 276 535.2 0 276 155 190 535.2 0 190 156 1696 535.2 0 1696 157 821 535.2 0 821 158 1342 535.2 0 1342 159 290 535.2 0 290 160 316 535.2 0 316 161 378 535.2 0 378 162 615 535.2 0 615 163 1297 535.2 0 1297 164 13081 535.2 0 13081 165 10988 535.2 0 10988 166 8713 535.2 0 8713 167 224 535.2 0 224 168 250 535.2 0 250 169 732 535.2 0 732 170 1384 535.2 0 1384 171 2347 535.2 0 2347 172 1598 535.2 0 1598 173 528 535.2 0 528 174 692 535.2 0 692 175 233 535.2 0 233 176 89 535.2 0 89 177 104 535.2 0 104 178 97 535.2 0 97 179 334 535.2 0 334 180 282 535.2 0 282 181 359 535.2 0 359 182 164 535.2 0 164 183 175 535.2 0 175 184 362 535.2 0 362 185 328 535.2 0 328 186 335 535.2 0 335 187 170 535.2 0 170 188 161 535.2 0 161 189 641 535.2 0 641 190 1775 535.2 0 1775 191 1440 535.2 0 1440 192 587 535.2 0 587 193 111 535.2 0 111 194 101 535.2 0 101 195 88 535.2 0 88 196 104 535.2 0 104 197 221 535.2 0 221 198 244 535.2 0 244 199 178 535.2 0 178 200 114 535.2 0 114 201 91 535.2 0 91 202 99 535.2 0 99 203 177 535.2 0 177 204 159 535.2 0 159 205 140 535.2 0 140 206 129 535.2 0 129 207 130 535.2 0 130 208 161 535.2 0 161 209 99 535.2 0 99 210 95 535.2 0 95 211 91 535.2 0 91 212 127 535.2 0 127 213 155 535.2 0 155 214 179 535.2 0 179 215 202 535.2 0 202 216 309 535.2 0 309 217 346 535.2 0 346 218 296 535.2 0 296 219 121 535.2 0 121 220 89 535.2 0 89 221 74 535.2 0 74 222 89 535.2 0 89 223 89 535.2 0 89 224 128 535.2 0 128 225 154 535.2 0 154 226 219 535.2 0 219 227 298 535.2 0 298 228 265 535.2 0 265 229 309 535.2 0 309 230 188 535.2 0 188 231 197 535.2 0 197 232 105 535.2 0 105 233 106 535.2 0 106 234 102 535.2 0 102 235 113 535.2 0 113 236 157 535.2 0 157 237 173 535.2 0 173 238 233 535.2 0 233 239 137 535.2 0 137 240 166 535.2 0 166 241 126 535.2 0 126 242 125 535.2 0 125 243 127 535.2 0 127 244 148 535.2 0 148 245 184 535.2 0 184 246 141 535.2 0 141 247 196 535.2 0 196 248 189 535.2 0 189 249 151 535.2 0 151 250 91 535.2 0 91 251 120 535.2 0 120 252 148 535.2 0 148 253 117 535.2 0 117 254 104 535.2 0 104 255 76 535.2 0 76 256 95 535.2 0 95 257 87 535.2 0 87 258 123 535.2 0 123 259 170 535.2 0 170 260 350 535.2 0 350 261 386 535.2 0 386 262 379 535.2 0 379 263 164 535.2 0 164 264 153 535.2 0 153 265 202 535.2 0 202 266 252 535.2 0 252 267 252 535.2 0 252 268 201 535.2 0 201 269 111 535.2 0 111 270 141 535.2 0 141 271 176 535.2 0 176 272 233 535.2 0 233 273 184 535.2 0 184 274 131 535.2 0 131 275 134 535.2 0 134 276 132 535.2 0 132 277 584 535.2 0 584 278 357 535.2 0 357 279 326 535.2 0 326 280 198 535.2 0 198 281 160 535.2 0 160 282 134 535.2 0 134 283 186 535.2 0 186 284 146 535.2 0 146 285 193 535.2 0 193 286 307 535.2 0 307 287 898 535.2 0 898 288 782 535.2 0 782 289 771 535.2 0 771 290 317 535.2 0 317 291 174 535.2 0 174 292 165 535.2 0 165 293 201 535.2 0 201 294 162 535.2 0 162 295 139 535.2 0 139 296 157 535.2 0 157 297 199 535.2 0 199 298 233 535.2 0 233 299 176 535.2 0 176 300 109 535.2 0 109 301 150 535.2 0 150 Finished in 15.29 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 417,026 Reads with adapters: 13,356 (3.2%) Reads that were too short: 1,390 (0.3%) Reads written (passing filters): 11,966 (2.9%) Total basepairs processed: 92,157,182 bp Total written (filtered): 2,792,420 bp (3.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13356 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 7 0.0 3 0 0 5 2 16 107 0.0 3 0 83 15 9 17 8583 0.0 3 7780 642 131 30 18 33 0.0 3 2 20 2 9 19 28 0.0 3 1 0 14 13 20 33 0.0 3 0 0 0 33 21 22 0.0 3 0 0 0 22 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 25 2 0.0 3 1 0 0 1 26 7 0.0 3 0 0 1 6 27 2 0.0 3 1 0 0 1 28 28 0.0 3 0 0 0 28 29 10 0.0 3 0 0 0 10 30 4 0.0 3 0 0 0 4 31 1 0.0 3 0 0 0 1 32 4 0.0 3 0 0 1 3 33 8 0.0 3 0 0 0 8 34 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 90 0.0 3 0 0 0 90 40 3 0.0 3 0 0 0 3 41 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 6 0.0 3 1 0 0 5 48 3 0.0 3 0 0 0 3 50 3 0.0 3 0 0 0 3 51 3 0.0 3 0 0 1 2 52 1 0.0 3 0 0 0 1 53 6 0.0 3 0 0 0 6 54 2 0.0 3 0 0 0 2 55 6 0.0 3 0 0 0 6 56 3 0.0 3 0 0 0 3 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 7 0.0 3 0 0 0 7 60 17 0.0 3 0 0 0 17 61 9 0.0 3 0 0 0 9 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 5 0.0 3 0 0 0 5 65 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 1 3 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 79 3 0.0 3 0 0 0 3 80 4 0.0 3 0 0 0 4 81 8 0.0 3 0 0 1 7 82 4 0.0 3 0 0 0 4 83 3 0.0 3 0 0 0 3 84 9 0.0 3 0 0 0 9 85 4 0.0 3 0 0 0 4 86 4 0.0 3 0 0 0 4 87 1 0.0 3 0 0 0 1 89 5 0.0 3 0 0 1 4 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 3 0.0 3 2 0 0 1 94 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 96 4 0.0 3 0 0 0 4 97 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 101 5 0.0 3 0 0 0 5 103 5 0.0 3 0 0 0 5 104 42 0.0 3 0 0 2 40 105 6 0.0 3 0 0 0 6 106 2 0.0 3 0 0 0 2 107 17 0.0 3 0 0 1 16 108 8 0.0 3 0 0 0 8 109 33 0.0 3 0 0 0 33 110 5 0.0 3 0 0 0 5 111 5 0.0 3 0 0 0 5 112 17 0.0 3 0 0 0 17 114 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 116 10 0.0 3 0 0 1 9 117 6 0.0 3 0 0 1 5 118 6 0.0 3 0 0 1 5 119 5 0.0 3 1 0 0 4 120 6 0.0 3 0 0 0 6 121 2 0.0 3 0 0 0 2 122 9 0.0 3 0 0 0 9 123 15 0.0 3 0 0 1 14 124 19 0.0 3 0 0 0 19 125 1 0.0 3 0 0 0 1 126 5 0.0 3 0 0 0 5 127 4 0.0 3 0 0 0 4 128 42 0.0 3 0 0 0 42 129 2173 0.0 3 0 0 18 2155 130 104 0.0 3 0 0 3 101 131 8 0.0 3 0 0 0 8 132 364 0.0 3 0 0 12 352 133 20 0.0 3 0 0 0 20 134 3 0.0 3 0 0 0 3 135 5 0.0 3 0 0 1 4 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 142 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 0 1 144 12 0.0 3 0 0 0 12 145 13 0.0 3 0 0 1 12 146 2 0.0 3 0 0 0 2 147 6 0.0 3 0 0 0 6 148 5 0.0 3 0 0 0 5 149 5 0.0 3 0 0 0 5 150 2 0.0 3 0 0 0 2 151 5 0.0 3 0 0 0 5 152 7 0.0 3 0 0 1 6 153 25 0.0 3 0 0 1 24 154 8 0.0 3 0 0 1 7 155 77 0.0 3 0 0 2 75 156 15 0.0 3 0 0 0 15 157 3 0.0 3 0 0 0 3 160 4 0.0 3 0 0 0 4 161 2 0.0 3 0 0 0 2 164 24 0.0 3 0 0 0 24 165 3 0.0 3 0 0 1 2 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 1 173 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 179 6 0.0 3 0 0 0 6 180 141 0.0 3 0 0 5 136 181 18 0.0 3 0 0 0 18 182 16 0.0 3 0 0 0 16 183 4 0.0 3 0 0 0 4 184 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 65 0.0 3 0 0 1 64 190 13 0.0 3 0 0 0 13 191 2 0.0 3 0 0 0 2 192 7 0.0 3 0 0 0 7 193 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 1 201 5 0.0 3 0 0 1 4 202 3 0.0 3 0 0 0 3 203 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 7 0.0 3 0 0 0 7 207 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 0 1 211 4 0.0 3 0 0 0 4 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 215 3 0.0 3 0 0 0 3 216 4 0.0 3 0 0 0 4 218 2 0.0 3 0 0 0 2 219 6 0.0 3 0 0 1 5 220 19 0.0 3 0 0 4 15 221 13 0.0 3 0 0 0 13 222 3 0.0 3 0 0 0 3 223 3 0.0 3 0 0 0 3 226 1 0.0 3 0 0 1 229 18 0.0 3 0 0 0 18 230 157 0.0 3 0 0 5 152 231 411 0.0 3 0 0 24 387 232 21 0.0 3 0 0 2 19 233 3 0.0 3 0 0 0 3 234 5 0.0 3 0 0 0 5 236 6 0.0 3 0 0 0 6 237 1 0.0 3 0 0 1 238 2 0.0 3 0 0 0 2 239 3 0.0 3 0 0 0 3 240 1 0.0 3 0 0 0 1 241 8 0.0 3 0 0 0 8 242 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 11,966 Reads with adapters: 91 (0.8%) Reads written (passing filters): 11,966 (100.0%) Total basepairs processed: 2,792,420 bp Total written (filtered): 2,792,094 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 91 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.6% C: 8.8% G: 51.6% T: 11.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 48 187.0 0 48 4 38 46.7 0 38 5 4 11.7 0 4 10 1 0.0 1 0 1