This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.74 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 75,067 (3.4%) Reads that were too short: 14,243 (0.6%) Reads written (passing filters): 60,824 (2.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 12,726,359 bp (1.9%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 75067 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1484 535.2 0 1484 7 10458 535.2 0 10458 8 7723 535.2 0 7723 9 127 535.2 0 127 10 78 535.2 0 78 11 71 535.2 0 71 12 67 535.2 0 67 13 65 535.2 0 65 14 92 535.2 0 92 15 81 535.2 0 81 16 83 535.2 0 83 17 91 535.2 0 91 18 105 535.2 0 105 19 113 535.2 0 113 20 148 535.2 0 148 21 171 535.2 0 171 22 85 535.2 0 85 23 104 535.2 0 104 24 61 535.2 0 61 25 61 535.2 0 61 26 124 535.2 0 124 27 71 535.2 0 71 28 75 535.2 0 75 29 86 535.2 0 86 30 111 535.2 0 111 31 106 535.2 0 106 32 84 535.2 0 84 33 71 535.2 0 71 34 109 535.2 0 109 35 111 535.2 0 111 36 95 535.2 0 95 37 81 535.2 0 81 38 253 535.2 0 253 39 301 535.2 0 301 40 208 535.2 0 208 41 87 535.2 0 87 42 144 535.2 0 144 43 83 535.2 0 83 44 85 535.2 0 85 45 88 535.2 0 88 46 96 535.2 0 96 47 110 535.2 0 110 48 93 535.2 0 93 49 90 535.2 0 90 50 97 535.2 0 97 51 75 535.2 0 75 52 92 535.2 0 92 53 71 535.2 0 71 54 88 535.2 0 88 55 94 535.2 0 94 56 92 535.2 0 92 57 115 535.2 0 115 58 96 535.2 0 96 59 94 535.2 0 94 60 88 535.2 0 88 61 79 535.2 0 79 62 93 535.2 0 93 63 76 535.2 0 76 64 87 535.2 0 87 65 80 535.2 0 80 66 77 535.2 0 77 67 94 535.2 0 94 68 98 535.2 0 98 69 84 535.2 0 84 70 87 535.2 0 87 71 106 535.2 0 106 72 104 535.2 0 104 73 121 535.2 0 121 74 87 535.2 0 87 75 81 535.2 0 81 76 110 535.2 0 110 77 269 535.2 0 269 78 236 535.2 0 236 79 107 535.2 0 107 80 94 535.2 0 94 81 96 535.2 0 96 82 94 535.2 0 94 83 90 535.2 0 90 84 90 535.2 0 90 85 73 535.2 0 73 86 96 535.2 0 96 87 86 535.2 0 86 88 90 535.2 0 90 89 102 535.2 0 102 90 109 535.2 0 109 91 134 535.2 0 134 92 154 535.2 0 154 93 521 535.2 0 521 94 990 535.2 0 990 95 893 535.2 0 893 96 518 535.2 0 518 97 433 535.2 0 433 98 375 535.2 0 375 99 100 535.2 0 100 100 72 535.2 0 72 101 69 535.2 0 69 102 55 535.2 0 55 103 65 535.2 0 65 104 58 535.2 0 58 105 66 535.2 0 66 106 69 535.2 0 69 107 104 535.2 0 104 108 83 535.2 0 83 109 82 535.2 0 82 110 129 535.2 0 129 111 63 535.2 0 63 112 83 535.2 0 83 113 99 535.2 0 99 114 137 535.2 0 137 115 85 535.2 0 85 116 99 535.2 0 99 117 78 535.2 0 78 118 71 535.2 0 71 119 64 535.2 0 64 120 57 535.2 0 57 121 58 535.2 0 58 122 69 535.2 0 69 123 90 535.2 0 90 124 53 535.2 0 53 125 86 535.2 0 86 126 79 535.2 0 79 127 73 535.2 0 73 128 135 535.2 0 135 129 143 535.2 0 143 130 278 535.2 0 278 131 769 535.2 0 769 132 793 535.2 0 793 133 67 535.2 0 67 134 86 535.2 0 86 135 86 535.2 0 86 136 103 535.2 0 103 137 114 535.2 0 114 138 155 535.2 0 155 139 61 535.2 0 61 140 65 535.2 0 65 141 74 535.2 0 74 142 97 535.2 0 97 143 99 535.2 0 99 144 138 535.2 0 138 145 203 535.2 0 203 146 102 535.2 0 102 147 72 535.2 0 72 148 61 535.2 0 61 149 73 535.2 0 73 150 80 535.2 0 80 151 85 535.2 0 85 152 114 535.2 0 114 153 92 535.2 0 92 154 122 535.2 0 122 155 73 535.2 0 73 156 92 535.2 0 92 157 130 535.2 0 130 158 125 535.2 0 125 159 153 535.2 0 153 160 79 535.2 0 79 161 84 535.2 0 84 162 118 535.2 0 118 163 113 535.2 0 113 164 135 535.2 0 135 165 188 535.2 0 188 166 208 535.2 0 208 167 322 535.2 0 322 168 742 535.2 0 742 169 7258 535.2 0 7258 170 5864 535.2 0 5864 171 3028 535.2 0 3028 172 109 535.2 0 109 173 97 535.2 0 97 174 119 535.2 0 119 175 133 535.2 0 133 176 485 535.2 0 485 177 297 535.2 0 297 178 73 535.2 0 73 179 75 535.2 0 75 180 129 535.2 0 129 181 70 535.2 0 70 182 75 535.2 0 75 183 85 535.2 0 85 184 108 535.2 0 108 185 98 535.2 0 98 186 82 535.2 0 82 187 62 535.2 0 62 188 63 535.2 0 63 189 78 535.2 0 78 190 68 535.2 0 68 191 90 535.2 0 90 192 154 535.2 0 154 193 142 535.2 0 142 194 135 535.2 0 135 195 86 535.2 0 86 196 74 535.2 0 74 197 61 535.2 0 61 198 109 535.2 0 109 199 81 535.2 0 81 200 87 535.2 0 87 201 78 535.2 0 78 202 99 535.2 0 99 203 112 535.2 0 112 204 123 535.2 0 123 205 142 535.2 0 142 206 100 535.2 0 100 207 60 535.2 0 60 208 68 535.2 0 68 209 82 535.2 0 82 210 105 535.2 0 105 211 116 535.2 0 116 212 132 535.2 0 132 213 104 535.2 0 104 214 99 535.2 0 99 215 113 535.2 0 113 216 132 535.2 0 132 217 151 535.2 0 151 218 102 535.2 0 102 219 73 535.2 0 73 220 72 535.2 0 72 221 218 535.2 0 218 222 296 535.2 0 296 223 219 535.2 0 219 224 394 535.2 0 394 225 80 535.2 0 80 226 62 535.2 0 62 227 90 535.2 0 90 228 92 535.2 0 92 229 112 535.2 0 112 230 85 535.2 0 85 231 125 535.2 0 125 232 141 535.2 0 141 233 96 535.2 0 96 234 120 535.2 0 120 235 140 535.2 0 140 236 340 535.2 0 340 237 404 535.2 0 404 238 335 535.2 0 335 239 82 535.2 0 82 240 87 535.2 0 87 241 86 535.2 0 86 242 147 535.2 0 147 243 105 535.2 0 105 244 80 535.2 0 80 245 83 535.2 0 83 246 111 535.2 0 111 247 62 535.2 0 62 248 50 535.2 0 50 249 71 535.2 0 71 250 86 535.2 0 86 251 89 535.2 0 89 252 82 535.2 0 82 253 90 535.2 0 90 254 123 535.2 0 123 255 118 535.2 0 118 256 96 535.2 0 96 257 100 535.2 0 100 258 89 535.2 0 89 259 79 535.2 0 79 260 69 535.2 0 69 261 231 535.2 0 231 262 309 535.2 0 309 263 202 535.2 0 202 264 94 535.2 0 94 265 66 535.2 0 66 266 84 535.2 0 84 267 91 535.2 0 91 268 79 535.2 0 79 269 73 535.2 0 73 270 132 535.2 0 132 271 231 535.2 0 231 272 418 535.2 0 418 273 310 535.2 0 310 274 164 535.2 0 164 275 70 535.2 0 70 276 83 535.2 0 83 277 84 535.2 0 84 278 82 535.2 0 82 279 94 535.2 0 94 280 116 535.2 0 116 281 109 535.2 0 109 282 126 535.2 0 126 283 156 535.2 0 156 284 170 535.2 0 170 285 115 535.2 0 115 286 104 535.2 0 104 287 96 535.2 0 96 288 102 535.2 0 102 289 120 535.2 0 120 290 160 535.2 0 160 291 176 535.2 0 176 292 289 535.2 0 289 293 292 535.2 0 292 294 355 535.2 0 355 295 105 535.2 0 105 296 274 535.2 0 274 297 343 535.2 0 343 298 350 535.2 0 350 299 233 535.2 0 233 300 161 535.2 0 161 301 75 535.2 0 75 Finished in 2.51 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 60,824 Reads with adapters: 10,868 (17.9%) Reads that were too short: 73 (0.1%) Reads written (passing filters): 10,795 (17.7%) Total basepairs processed: 12,726,359 bp Total written (filtered): 2,980,217 bp (23.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10868 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 11 0.0 3 0 0 3 8 16 127 0.0 3 0 89 19 19 17 9813 0.0 3 8950 667 160 36 18 31 0.0 3 1 25 2 3 19 47 0.0 3 0 14 15 18 20 699 0.0 3 574 57 18 50 21 4 0.0 3 0 3 1 22 4 0.0 3 0 0 3 1 30 1 0.0 3 0 0 0 1 39 1 0.0 3 1 44 4 0.0 3 0 0 0 4 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 1 3 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 62 11 0.0 3 0 0 0 11 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 9 0.0 3 0 0 0 9 99 2 0.0 3 0 0 0 2 101 5 0.0 3 0 0 0 5 102 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 3 0.0 3 0 0 0 3 151 4 0.0 3 0 0 0 4 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 189 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 200 7 0.0 3 0 0 1 6 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 209 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 1 253 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 10,795 Reads with adapters: 54 (0.5%) Reads written (passing filters): 10,795 (100.0%) Total basepairs processed: 2,980,217 bp Total written (filtered): 2,980,048 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 54 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.5% C: 33.3% G: 33.3% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 50 168.7 0 50 4 2 42.2 0 2 5 1 10.5 0 1 6 1 2.6 0 1