This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.23 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 80,093 (3.7%) Reads that were too short: 11,821 (0.5%) Reads written (passing filters): 68,272 (3.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 13,681,189 bp (2.1%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 80093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1453 535.2 0 1453 7 9420 535.2 0 9420 8 10019 535.2 0 10019 9 101 535.2 0 101 10 107 535.2 0 107 11 81 535.2 0 81 12 89 535.2 0 89 13 66 535.2 0 66 14 80 535.2 0 80 15 85 535.2 0 85 16 67 535.2 0 67 17 87 535.2 0 87 18 110 535.2 0 110 19 131 535.2 0 131 20 124 535.2 0 124 21 120 535.2 0 120 22 64 535.2 0 64 23 109 535.2 0 109 24 52 535.2 0 52 25 81 535.2 0 81 26 104 535.2 0 104 27 92 535.2 0 92 28 80 535.2 0 80 29 89 535.2 0 89 30 97 535.2 0 97 31 94 535.2 0 94 32 81 535.2 0 81 33 77 535.2 0 77 34 95 535.2 0 95 35 104 535.2 0 104 36 101 535.2 0 101 37 94 535.2 0 94 38 183 535.2 0 183 39 225 535.2 0 225 40 103 535.2 0 103 41 98 535.2 0 98 42 103 535.2 0 103 43 97 535.2 0 97 44 105 535.2 0 105 45 108 535.2 0 108 46 95 535.2 0 95 47 98 535.2 0 98 48 96 535.2 0 96 49 113 535.2 0 113 50 95 535.2 0 95 51 96 535.2 0 96 52 79 535.2 0 79 53 86 535.2 0 86 54 107 535.2 0 107 55 74 535.2 0 74 56 97 535.2 0 97 57 125 535.2 0 125 58 109 535.2 0 109 59 93 535.2 0 93 60 94 535.2 0 94 61 81 535.2 0 81 62 92 535.2 0 92 63 72 535.2 0 72 64 90 535.2 0 90 65 75 535.2 0 75 66 97 535.2 0 97 67 75 535.2 0 75 68 91 535.2 0 91 69 79 535.2 0 79 70 83 535.2 0 83 71 106 535.2 0 106 72 61 535.2 0 61 73 106 535.2 0 106 74 103 535.2 0 103 75 88 535.2 0 88 76 106 535.2 0 106 77 220 535.2 0 220 78 225 535.2 0 225 79 84 535.2 0 84 80 99 535.2 0 99 81 93 535.2 0 93 82 91 535.2 0 91 83 61 535.2 0 61 84 100 535.2 0 100 85 73 535.2 0 73 86 93 535.2 0 93 87 75 535.2 0 75 88 95 535.2 0 95 89 75 535.2 0 75 90 102 535.2 0 102 91 100 535.2 0 100 92 104 535.2 0 104 93 197 535.2 0 197 94 392 535.2 0 392 95 331 535.2 0 331 96 190 535.2 0 190 97 160 535.2 0 160 98 172 535.2 0 172 99 65 535.2 0 65 100 80 535.2 0 80 101 69 535.2 0 69 102 80 535.2 0 80 103 67 535.2 0 67 104 47 535.2 0 47 105 58 535.2 0 58 106 83 535.2 0 83 107 78 535.2 0 78 108 91 535.2 0 91 109 71 535.2 0 71 110 71 535.2 0 71 111 89 535.2 0 89 112 73 535.2 0 73 113 97 535.2 0 97 114 120 535.2 0 120 115 84 535.2 0 84 116 84 535.2 0 84 117 130 535.2 0 130 118 68 535.2 0 68 119 58 535.2 0 58 120 50 535.2 0 50 121 58 535.2 0 58 122 72 535.2 0 72 123 83 535.2 0 83 124 61 535.2 0 61 125 91 535.2 0 91 126 84 535.2 0 84 127 74 535.2 0 74 128 138 535.2 0 138 129 108 535.2 0 108 130 322 535.2 0 322 131 859 535.2 0 859 132 830 535.2 0 830 133 98 535.2 0 98 134 106 535.2 0 106 135 111 535.2 0 111 136 106 535.2 0 106 137 85 535.2 0 85 138 141 535.2 0 141 139 72 535.2 0 72 140 81 535.2 0 81 141 96 535.2 0 96 142 102 535.2 0 102 143 103 535.2 0 103 144 118 535.2 0 118 145 178 535.2 0 178 146 113 535.2 0 113 147 72 535.2 0 72 148 64 535.2 0 64 149 65 535.2 0 65 150 92 535.2 0 92 151 107 535.2 0 107 152 175 535.2 0 175 153 98 535.2 0 98 154 121 535.2 0 121 155 71 535.2 0 71 156 76 535.2 0 76 157 121 535.2 0 121 158 185 535.2 0 185 159 155 535.2 0 155 160 85 535.2 0 85 161 77 535.2 0 77 162 115 535.2 0 115 163 109 535.2 0 109 164 160 535.2 0 160 165 176 535.2 0 176 166 199 535.2 0 199 167 419 535.2 0 419 168 978 535.2 0 978 169 11129 535.2 0 11129 170 8719 535.2 0 8719 171 4595 535.2 0 4595 172 104 535.2 0 104 173 78 535.2 0 78 174 129 535.2 0 129 175 153 535.2 0 153 176 675 535.2 0 675 177 398 535.2 0 398 178 83 535.2 0 83 179 73 535.2 0 73 180 89 535.2 0 89 181 72 535.2 0 72 182 78 535.2 0 78 183 77 535.2 0 77 184 97 535.2 0 97 185 84 535.2 0 84 186 57 535.2 0 57 187 68 535.2 0 68 188 62 535.2 0 62 189 69 535.2 0 69 190 81 535.2 0 81 191 82 535.2 0 82 192 167 535.2 0 167 193 118 535.2 0 118 194 94 535.2 0 94 195 76 535.2 0 76 196 64 535.2 0 64 197 66 535.2 0 66 198 83 535.2 0 83 199 67 535.2 0 67 200 97 535.2 0 97 201 95 535.2 0 95 202 95 535.2 0 95 203 117 535.2 0 117 204 112 535.2 0 112 205 139 535.2 0 139 206 85 535.2 0 85 207 77 535.2 0 77 208 82 535.2 0 82 209 72 535.2 0 72 210 81 535.2 0 81 211 83 535.2 0 83 212 69 535.2 0 69 213 88 535.2 0 88 214 66 535.2 0 66 215 88 535.2 0 88 216 65 535.2 0 65 217 87 535.2 0 87 218 80 535.2 0 80 219 56 535.2 0 56 220 65 535.2 0 65 221 289 535.2 0 289 222 365 535.2 0 365 223 268 535.2 0 268 224 483 535.2 0 483 225 80 535.2 0 80 226 66 535.2 0 66 227 72 535.2 0 72 228 81 535.2 0 81 229 75 535.2 0 75 230 60 535.2 0 60 231 115 535.2 0 115 232 115 535.2 0 115 233 75 535.2 0 75 234 88 535.2 0 88 235 96 535.2 0 96 236 139 535.2 0 139 237 186 535.2 0 186 238 195 535.2 0 195 239 101 535.2 0 101 240 85 535.2 0 85 241 82 535.2 0 82 242 100 535.2 0 100 243 78 535.2 0 78 244 68 535.2 0 68 245 69 535.2 0 69 246 124 535.2 0 124 247 75 535.2 0 75 248 60 535.2 0 60 249 57 535.2 0 57 250 66 535.2 0 66 251 71 535.2 0 71 252 67 535.2 0 67 253 69 535.2 0 69 254 82 535.2 0 82 255 93 535.2 0 93 256 70 535.2 0 70 257 73 535.2 0 73 258 68 535.2 0 68 259 81 535.2 0 81 260 76 535.2 0 76 261 156 535.2 0 156 262 187 535.2 0 187 263 92 535.2 0 92 264 80 535.2 0 80 265 76 535.2 0 76 266 76 535.2 0 76 267 83 535.2 0 83 268 92 535.2 0 92 269 86 535.2 0 86 270 144 535.2 0 144 271 291 535.2 0 291 272 570 535.2 0 570 273 463 535.2 0 463 274 260 535.2 0 260 275 74 535.2 0 74 276 65 535.2 0 65 277 93 535.2 0 93 278 72 535.2 0 72 279 107 535.2 0 107 280 88 535.2 0 88 281 91 535.2 0 91 282 83 535.2 0 83 283 144 535.2 0 144 284 134 535.2 0 134 285 77 535.2 0 77 286 91 535.2 0 91 287 65 535.2 0 65 288 70 535.2 0 70 289 100 535.2 0 100 290 91 535.2 0 91 291 126 535.2 0 126 292 183 535.2 0 183 293 186 535.2 0 186 294 179 535.2 0 179 295 95 535.2 0 95 296 148 535.2 0 148 297 208 535.2 0 208 298 166 535.2 0 166 299 103 535.2 0 103 300 83 535.2 0 83 301 73 535.2 0 73 Finished in 2.87 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 68,272 Reads with adapters: 11,203 (16.4%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 11,193 (16.4%) Total basepairs processed: 13,681,189 bp Total written (filtered): 3,049,614 bp (22.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11203 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 1 6 18 5 0.0 3 0 0 5 19 110 0.0 3 0 109 1 20 10598 0.0 4 10453 141 4 21 20 0.0 4 3 17 22 9 0.0 4 1 6 2 23 381 0.0 4 370 5 0 6 24 1 0.0 4 0 1 41 22 0.0 4 0 0 0 0 22 42 5 0.0 4 0 0 0 0 5 44 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 92 1 0.0 4 1 110 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 27 0.0 4 0 0 0 0 27 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 184 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 11,193 Reads with adapters: 1,314 (11.7%) Reads written (passing filters): 11,193 (100.0%) Total basepairs processed: 3,049,614 bp Total written (filtered): 3,034,235 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1314 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.4% C: 4.0% G: 93.5% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 174.9 0 9 4 116 43.7 0 116 7 2 0.7 0 2 9 30 0.0 0 0 30 10 74 0.0 1 0 74 11 1068 0.0 1 3 1065 12 5 0.0 1 1 4 150 1 0.0 1 0 1 151 2 0.0 1 0 2 209 1 0.0 1 0 1 224 3 0.0 1 3 225 1 0.0 1 1 226 1 0.0 1 1 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.