This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.63 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 73,754 (3.4%) Reads that were too short: 9,040 (0.4%) Reads written (passing filters): 64,714 (3.0%) Total basepairs processed: 659,880,795 bp Total written (filtered): 17,456,712 bp (2.6%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 73754 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2817 535.2 0 2817 7 3960 535.2 0 3960 8 26574 535.2 0 26574 9 18161 535.2 0 18161 10 60 535.2 0 60 11 58 535.2 0 58 12 19 535.2 0 19 13 30 535.2 0 30 14 23 535.2 0 23 15 42 535.2 0 42 16 19 535.2 0 19 17 13 535.2 0 13 18 25 535.2 0 25 19 21 535.2 0 21 20 25 535.2 0 25 21 41 535.2 0 41 22 42 535.2 0 42 23 25 535.2 0 25 24 18 535.2 0 18 25 29 535.2 0 29 26 22 535.2 0 22 27 31 535.2 0 31 28 29 535.2 0 29 29 21 535.2 0 21 30 26 535.2 0 26 31 16 535.2 0 16 32 13 535.2 0 13 33 25 535.2 0 25 34 29 535.2 0 29 35 44 535.2 0 44 36 23 535.2 0 23 37 39 535.2 0 39 38 71 535.2 0 71 39 28 535.2 0 28 40 22 535.2 0 22 41 43 535.2 0 43 42 32 535.2 0 32 43 38 535.2 0 38 44 30 535.2 0 30 45 44 535.2 0 44 46 33 535.2 0 33 47 90 535.2 0 90 48 658 535.2 0 658 49 536 535.2 0 536 50 763 535.2 0 763 51 54 535.2 0 54 52 30 535.2 0 30 53 24 535.2 0 24 54 24 535.2 0 24 55 10 535.2 0 10 56 32 535.2 0 32 57 17 535.2 0 17 58 14 535.2 0 14 59 19 535.2 0 19 60 26 535.2 0 26 61 29 535.2 0 29 62 22 535.2 0 22 63 14 535.2 0 14 64 27 535.2 0 27 65 24 535.2 0 24 66 25 535.2 0 25 67 23 535.2 0 23 68 21 535.2 0 21 69 21 535.2 0 21 70 10 535.2 0 10 71 20 535.2 0 20 72 13 535.2 0 13 73 13 535.2 0 13 74 45 535.2 0 45 75 24 535.2 0 24 76 44 535.2 0 44 77 19 535.2 0 19 78 29 535.2 0 29 79 29 535.2 0 29 80 39 535.2 0 39 81 125 535.2 0 125 82 122 535.2 0 122 83 261 535.2 0 261 84 56 535.2 0 56 85 43 535.2 0 43 86 19 535.2 0 19 87 33 535.2 0 33 88 49 535.2 0 49 89 52 535.2 0 52 90 63 535.2 0 63 91 36 535.2 0 36 92 21 535.2 0 21 93 18 535.2 0 18 94 25 535.2 0 25 95 22 535.2 0 22 96 65 535.2 0 65 97 124 535.2 0 124 98 195 535.2 0 195 99 26 535.2 0 26 100 21 535.2 0 21 101 18 535.2 0 18 102 14 535.2 0 14 103 17 535.2 0 17 104 26 535.2 0 26 105 28 535.2 0 28 106 15 535.2 0 15 107 23 535.2 0 23 108 25 535.2 0 25 109 35 535.2 0 35 110 21 535.2 0 21 111 19 535.2 0 19 112 28 535.2 0 28 113 56 535.2 0 56 114 67 535.2 0 67 115 38 535.2 0 38 116 35 535.2 0 35 117 40 535.2 0 40 118 134 535.2 0 134 119 189 535.2 0 189 120 204 535.2 0 204 121 25 535.2 0 25 122 38 535.2 0 38 123 26 535.2 0 26 124 26 535.2 0 26 125 25 535.2 0 25 126 37 535.2 0 37 127 70 535.2 0 70 128 226 535.2 0 226 129 167 535.2 0 167 130 28 535.2 0 28 131 32 535.2 0 32 132 41 535.2 0 41 133 35 535.2 0 35 134 52 535.2 0 52 135 32 535.2 0 32 136 61 535.2 0 61 137 79 535.2 0 79 138 106 535.2 0 106 139 43 535.2 0 43 140 22 535.2 0 22 141 36 535.2 0 36 142 45 535.2 0 45 143 45 535.2 0 45 144 66 535.2 0 66 145 74 535.2 0 74 146 221 535.2 0 221 147 96 535.2 0 96 148 377 535.2 0 377 149 29 535.2 0 29 150 24 535.2 0 24 151 56 535.2 0 56 152 30 535.2 0 30 153 77 535.2 0 77 154 41 535.2 0 41 155 79 535.2 0 79 156 26 535.2 0 26 157 39 535.2 0 39 158 54 535.2 0 54 159 23 535.2 0 23 160 17 535.2 0 17 161 21 535.2 0 21 162 26 535.2 0 26 163 22 535.2 0 22 164 24 535.2 0 24 165 25 535.2 0 25 166 26 535.2 0 26 167 20 535.2 0 20 168 41 535.2 0 41 169 20 535.2 0 20 170 48 535.2 0 48 171 34 535.2 0 34 172 39 535.2 0 39 173 27 535.2 0 27 174 9 535.2 0 9 175 17 535.2 0 17 176 37 535.2 0 37 177 28 535.2 0 28 178 52 535.2 0 52 179 39 535.2 0 39 180 37 535.2 0 37 181 46 535.2 0 46 182 97 535.2 0 97 183 45 535.2 0 45 184 26 535.2 0 26 185 25 535.2 0 25 186 27 535.2 0 27 187 31 535.2 0 31 188 34 535.2 0 34 189 57 535.2 0 57 190 72 535.2 0 72 191 80 535.2 0 80 192 70 535.2 0 70 193 99 535.2 0 99 194 183 535.2 0 183 195 879 535.2 0 879 196 857 535.2 0 857 197 871 535.2 0 871 198 51 535.2 0 51 199 27 535.2 0 27 200 27 535.2 0 27 201 35 535.2 0 35 202 48 535.2 0 48 203 34 535.2 0 34 204 36 535.2 0 36 205 16 535.2 0 16 206 31 535.2 0 31 207 26 535.2 0 26 208 38 535.2 0 38 209 43 535.2 0 43 210 35 535.2 0 35 211 30 535.2 0 30 212 51 535.2 0 51 213 72 535.2 0 72 214 146 535.2 0 146 215 163 535.2 0 163 216 116 535.2 0 116 217 37 535.2 0 37 218 51 535.2 0 51 219 40 535.2 0 40 220 66 535.2 0 66 221 63 535.2 0 63 222 40 535.2 0 40 223 49 535.2 0 49 224 47 535.2 0 47 225 46 535.2 0 46 226 41 535.2 0 41 227 32 535.2 0 32 228 27 535.2 0 27 229 38 535.2 0 38 230 58 535.2 0 58 231 76 535.2 0 76 232 45 535.2 0 45 233 73 535.2 0 73 234 90 535.2 0 90 235 85 535.2 0 85 236 126 535.2 0 126 237 58 535.2 0 58 238 57 535.2 0 57 239 125 535.2 0 125 240 144 535.2 0 144 241 115 535.2 0 115 242 77 535.2 0 77 243 280 535.2 0 280 244 232 535.2 0 232 245 326 535.2 0 326 246 88 535.2 0 88 247 56 535.2 0 56 248 53 535.2 0 53 249 95 535.2 0 95 250 139 535.2 0 139 251 158 535.2 0 158 252 145 535.2 0 145 253 111 535.2 0 111 254 127 535.2 0 127 255 100 535.2 0 100 256 81 535.2 0 81 257 74 535.2 0 74 258 81 535.2 0 81 259 75 535.2 0 75 260 118 535.2 0 118 261 221 535.2 0 221 262 167 535.2 0 167 263 152 535.2 0 152 264 85 535.2 0 85 265 74 535.2 0 74 266 64 535.2 0 64 267 35 535.2 0 35 268 46 535.2 0 46 269 105 535.2 0 105 270 158 535.2 0 158 271 114 535.2 0 114 272 46 535.2 0 46 273 60 535.2 0 60 274 42 535.2 0 42 275 62 535.2 0 62 276 46 535.2 0 46 277 49 535.2 0 49 278 41 535.2 0 41 279 37 535.2 0 37 280 50 535.2 0 50 281 65 535.2 0 65 282 70 535.2 0 70 283 53 535.2 0 53 284 93 535.2 0 93 285 56 535.2 0 56 286 98 535.2 0 98 287 125 535.2 0 125 288 140 535.2 0 140 289 211 535.2 0 211 290 157 535.2 0 157 291 160 535.2 0 160 292 94 535.2 0 94 293 118 535.2 0 118 294 80 535.2 0 80 295 82 535.2 0 82 296 100 535.2 0 100 297 203 535.2 0 203 298 178 535.2 0 178 299 230 535.2 0 230 300 107 535.2 0 107 301 36 535.2 0 36 Finished in 3.18 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 64,714 Reads with adapters: 27,529 (42.5%) Reads that were too short: 88 (0.1%) Reads written (passing filters): 27,441 (42.4%) Total basepairs processed: 17,456,712 bp Total written (filtered): 7,551,440 bp (43.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 27529 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 14 0.0 2 0 0 0 14 15 18 0.0 3 0 0 13 5 16 245 0.0 3 0 186 38 21 17 24600 0.0 3 22367 1783 345 105 18 116 0.0 3 2 81 13 20 19 2186 0.0 3 1894 173 78 41 20 95 0.0 3 0 4 2 89 21 13 0.0 3 0 0 6 7 22 4 0.0 3 0 0 0 4 25 1 0.0 3 0 0 0 1 27 1 0.0 3 1 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 35 5 0.0 3 0 0 0 5 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 4 0.0 3 0 0 1 3 44 2 0.0 3 0 0 0 2 45 14 0.0 3 0 0 1 13 48 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 9 0.0 3 0 0 0 9 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 1 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 13 0.0 3 0 0 0 13 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 4 0.0 3 0 0 0 4 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 1 2 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 1 112 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 6 0.0 3 0 0 0 6 124 2 0.0 3 0 0 0 2 126 3 0.0 3 0 0 0 3 136 3 0.0 3 0 0 1 2 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 143 24 0.0 3 0 0 0 24 144 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 1 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 3 0.0 3 0 0 1 2 189 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 195 2 0.0 3 0 0 0 2 198 3 0.0 3 0 0 0 3 200 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 2 0.0 3 0 0 0 2 244 2 0.0 3 0 0 1 1 245 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 2 0.0 3 0 0 0 2 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.95 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 27,441 Reads with adapters: 67 (0.2%) Reads written (passing filters): 27,441 (100.0%) Total basepairs processed: 7,551,440 bp Total written (filtered): 7,551,211 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 67 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.9% C: 19.4% G: 43.3% T: 16.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 47 428.8 0 47 4 13 107.2 0 13 5 6 26.8 0 6 6 1 6.7 0 1