This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.57 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 64,478 (2.9%) Reads that were too short: 3,528 (0.2%) Reads written (passing filters): 60,950 (2.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 17,183,207 bp (2.6%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 64478 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2800 535.2 0 2800 7 4552 535.2 0 4552 8 23305 535.2 0 23305 9 24604 535.2 0 24604 10 41 535.2 0 41 11 22 535.2 0 22 12 12 535.2 0 12 13 10 535.2 0 10 14 15 535.2 0 15 15 17 535.2 0 17 16 12 535.2 0 12 17 9 535.2 0 9 18 15 535.2 0 15 19 8 535.2 0 8 20 9 535.2 0 9 21 11 535.2 0 11 22 23 535.2 0 23 23 16 535.2 0 16 24 11 535.2 0 11 25 11 535.2 0 11 26 14 535.2 0 14 27 16 535.2 0 16 28 12 535.2 0 12 29 5 535.2 0 5 30 24 535.2 0 24 31 13 535.2 0 13 32 12 535.2 0 12 33 12 535.2 0 12 34 19 535.2 0 19 35 17 535.2 0 17 36 21 535.2 0 21 37 21 535.2 0 21 38 21 535.2 0 21 39 18 535.2 0 18 40 13 535.2 0 13 41 15 535.2 0 15 42 15 535.2 0 15 43 20 535.2 0 20 44 7 535.2 0 7 45 22 535.2 0 22 46 24 535.2 0 24 47 18 535.2 0 18 48 126 535.2 0 126 49 181 535.2 0 181 50 233 535.2 0 233 51 9 535.2 0 9 52 9 535.2 0 9 53 9 535.2 0 9 54 9 535.2 0 9 55 16 535.2 0 16 56 16 535.2 0 16 57 9 535.2 0 9 58 8 535.2 0 8 59 17 535.2 0 17 60 8 535.2 0 8 61 19 535.2 0 19 62 11 535.2 0 11 63 9 535.2 0 9 64 12 535.2 0 12 65 14 535.2 0 14 66 12 535.2 0 12 67 15 535.2 0 15 68 15 535.2 0 15 69 11 535.2 0 11 70 8 535.2 0 8 71 22 535.2 0 22 72 9 535.2 0 9 73 11 535.2 0 11 74 14 535.2 0 14 75 9 535.2 0 9 76 8 535.2 0 8 77 9 535.2 0 9 78 13 535.2 0 13 79 13 535.2 0 13 80 18 535.2 0 18 81 24 535.2 0 24 82 26 535.2 0 26 83 55 535.2 0 55 84 18 535.2 0 18 85 41 535.2 0 41 86 15 535.2 0 15 87 11 535.2 0 11 88 24 535.2 0 24 89 22 535.2 0 22 90 49 535.2 0 49 91 14 535.2 0 14 92 14 535.2 0 14 93 15 535.2 0 15 94 16 535.2 0 16 95 15 535.2 0 15 96 22 535.2 0 22 97 27 535.2 0 27 98 37 535.2 0 37 99 21 535.2 0 21 100 12 535.2 0 12 101 8 535.2 0 8 102 3 535.2 0 3 103 8 535.2 0 8 104 17 535.2 0 17 105 11 535.2 0 11 106 9 535.2 0 9 107 11 535.2 0 11 108 10 535.2 0 10 109 16 535.2 0 16 110 14 535.2 0 14 111 7 535.2 0 7 112 13 535.2 0 13 113 20 535.2 0 20 114 26 535.2 0 26 115 17 535.2 0 17 116 20 535.2 0 20 117 12 535.2 0 12 118 42 535.2 0 42 119 43 535.2 0 43 120 62 535.2 0 62 121 10 535.2 0 10 122 23 535.2 0 23 123 12 535.2 0 12 124 17 535.2 0 17 125 12 535.2 0 12 126 11 535.2 0 11 127 28 535.2 0 28 128 73 535.2 0 73 129 46 535.2 0 46 130 17 535.2 0 17 131 13 535.2 0 13 132 22 535.2 0 22 133 22 535.2 0 22 134 18 535.2 0 18 135 28 535.2 0 28 136 24 535.2 0 24 137 36 535.2 0 36 138 43 535.2 0 43 139 24 535.2 0 24 140 20 535.2 0 20 141 22 535.2 0 22 142 15 535.2 0 15 143 19 535.2 0 19 144 21 535.2 0 21 145 40 535.2 0 40 146 257 535.2 0 257 147 94 535.2 0 94 148 395 535.2 0 395 149 22 535.2 0 22 150 17 535.2 0 17 151 11 535.2 0 11 152 14 535.2 0 14 153 86 535.2 0 86 154 30 535.2 0 30 155 47 535.2 0 47 156 16 535.2 0 16 157 13 535.2 0 13 158 13 535.2 0 13 159 14 535.2 0 14 160 19 535.2 0 19 161 16 535.2 0 16 162 15 535.2 0 15 163 15 535.2 0 15 164 15 535.2 0 15 165 19 535.2 0 19 166 19 535.2 0 19 167 23 535.2 0 23 168 18 535.2 0 18 169 24 535.2 0 24 170 24 535.2 0 24 171 21 535.2 0 21 172 17 535.2 0 17 173 13 535.2 0 13 174 6 535.2 0 6 175 19 535.2 0 19 176 24 535.2 0 24 177 29 535.2 0 29 178 21 535.2 0 21 179 31 535.2 0 31 180 14 535.2 0 14 181 40 535.2 0 40 182 76 535.2 0 76 183 41 535.2 0 41 184 9 535.2 0 9 185 20 535.2 0 20 186 13 535.2 0 13 187 17 535.2 0 17 188 29 535.2 0 29 189 25 535.2 0 25 190 30 535.2 0 30 191 36 535.2 0 36 192 42 535.2 0 42 193 48 535.2 0 48 194 45 535.2 0 45 195 274 535.2 0 274 196 228 535.2 0 228 197 255 535.2 0 255 198 23 535.2 0 23 199 8 535.2 0 8 200 19 535.2 0 19 201 14 535.2 0 14 202 16 535.2 0 16 203 20 535.2 0 20 204 17 535.2 0 17 205 14 535.2 0 14 206 10 535.2 0 10 207 16 535.2 0 16 208 12 535.2 0 12 209 18 535.2 0 18 210 16 535.2 0 16 211 16 535.2 0 16 212 20 535.2 0 20 213 19 535.2 0 19 214 29 535.2 0 29 215 38 535.2 0 38 216 41 535.2 0 41 217 13 535.2 0 13 218 25 535.2 0 25 219 20 535.2 0 20 220 15 535.2 0 15 221 34 535.2 0 34 222 18 535.2 0 18 223 18 535.2 0 18 224 10 535.2 0 10 225 18 535.2 0 18 226 15 535.2 0 15 227 10 535.2 0 10 228 11 535.2 0 11 229 11 535.2 0 11 230 15 535.2 0 15 231 24 535.2 0 24 232 23 535.2 0 23 233 20 535.2 0 20 234 27 535.2 0 27 235 25 535.2 0 25 236 120 535.2 0 120 237 24 535.2 0 24 238 21 535.2 0 21 239 35 535.2 0 35 240 31 535.2 0 31 241 29 535.2 0 29 242 22 535.2 0 22 243 96 535.2 0 96 244 59 535.2 0 59 245 80 535.2 0 80 246 26 535.2 0 26 247 20 535.2 0 20 248 19 535.2 0 19 249 24 535.2 0 24 250 33 535.2 0 33 251 114 535.2 0 114 252 51 535.2 0 51 253 40 535.2 0 40 254 27 535.2 0 27 255 83 535.2 0 83 256 29 535.2 0 29 257 19 535.2 0 19 258 26 535.2 0 26 259 35 535.2 0 35 260 34 535.2 0 34 261 75 535.2 0 75 262 50 535.2 0 50 263 44 535.2 0 44 264 22 535.2 0 22 265 27 535.2 0 27 266 19 535.2 0 19 267 20 535.2 0 20 268 22 535.2 0 22 269 29 535.2 0 29 270 32 535.2 0 32 271 33 535.2 0 33 272 23 535.2 0 23 273 18 535.2 0 18 274 19 535.2 0 19 275 30 535.2 0 30 276 27 535.2 0 27 277 15 535.2 0 15 278 15 535.2 0 15 279 18 535.2 0 18 280 12 535.2 0 12 281 14 535.2 0 14 282 20 535.2 0 20 283 19 535.2 0 19 284 35 535.2 0 35 285 20 535.2 0 20 286 43 535.2 0 43 287 114 535.2 0 114 288 128 535.2 0 128 289 106 535.2 0 106 290 82 535.2 0 82 291 80 535.2 0 80 292 47 535.2 0 47 293 35 535.2 0 35 294 35 535.2 0 35 295 28 535.2 0 28 296 38 535.2 0 38 297 74 535.2 0 74 298 68 535.2 0 68 299 84 535.2 0 84 300 128 535.2 0 128 301 29 535.2 0 29 Finished in 3.20 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 60,950 Reads with adapters: 30,767 (50.5%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 30,759 (50.5%) Total basepairs processed: 17,183,207 bp Total written (filtered): 8,364,608 bp (48.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30767 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 18 0.0 3 0 0 17 1 19 418 0.0 3 0 412 2 1 3 20 26061 0.0 4 25616 432 12 1 21 133 0.0 4 22 104 6 1 22 4032 0.0 4 3963 56 9 2 2 23 44 0.0 4 1 12 0 29 2 24 1 0.0 4 0 0 0 0 1 25 6 0.0 4 1 0 0 5 26 3 0.0 4 1 0 0 1 1 28 1 0.0 4 1 39 1 0.0 4 1 43 1 0.0 4 1 64 1 0.0 4 0 0 0 0 1 79 1 0.0 4 1 86 2 0.0 4 0 0 0 0 2 112 2 0.0 4 0 1 0 0 1 127 35 0.0 4 0 0 0 0 35 139 1 0.0 4 0 1 164 1 0.0 4 1 228 2 0.0 4 0 0 0 1 1 233 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.08 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 30,759 Reads with adapters: 4,248 (13.8%) Reads written (passing filters): 30,759 (100.0%) Total basepairs processed: 8,364,608 bp Total written (filtered): 8,320,529 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4248 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.1% G: 95.0% T: 1.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 480.6 0 11 4 7 120.2 0 7 5 2 30.0 0 2 6 5 7.5 0 5 7 1 1.9 0 1 8 3 0.5 0 3 9 1788 0.1 0 48 1740 10 1928 0.0 1 76 1852 11 474 0.0 1 9 465 12 4 0.0 1 0 4 13 4 0.0 1 0 4 78 1 0.0 1 1 150 13 0.0 1 0 13 151 2 0.0 1 0 2 152 2 0.0 1 0 2 208 2 0.0 1 1 1 211 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.