This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.32 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 79,380 (3.6%) Reads that were too short: 28,254 (1.3%) Reads written (passing filters): 51,126 (2.3%) Total basepairs processed: 659,880,795 bp Total written (filtered): 11,671,177 bp (1.8%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 79380 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1226 535.2 0 1226 7 8464 535.2 0 8464 8 7374 535.2 0 7374 9 130 535.2 0 130 10 580 535.2 0 580 11 55 535.2 0 55 12 52 535.2 0 52 13 59 535.2 0 59 14 74 535.2 0 74 15 62 535.2 0 62 16 62 535.2 0 62 17 86 535.2 0 86 18 99 535.2 0 99 19 144 535.2 0 144 20 56 535.2 0 56 21 57 535.2 0 57 22 32 535.2 0 32 23 53 535.2 0 53 24 49 535.2 0 49 25 64 535.2 0 64 26 66 535.2 0 66 27 68 535.2 0 68 28 65 535.2 0 65 29 90 535.2 0 90 30 76 535.2 0 76 31 49 535.2 0 49 32 58 535.2 0 58 33 74 535.2 0 74 34 53 535.2 0 53 35 71 535.2 0 71 36 65 535.2 0 65 37 56 535.2 0 56 38 47 535.2 0 47 39 62 535.2 0 62 40 81 535.2 0 81 41 37 535.2 0 37 42 76 535.2 0 76 43 85 535.2 0 85 44 42 535.2 0 42 45 47 535.2 0 47 46 76 535.2 0 76 47 93 535.2 0 93 48 99 535.2 0 99 49 149 535.2 0 149 50 84 535.2 0 84 51 66 535.2 0 66 52 120 535.2 0 120 53 193 535.2 0 193 54 185 535.2 0 185 55 87 535.2 0 87 56 137 535.2 0 137 57 130 535.2 0 130 58 112 535.2 0 112 59 73 535.2 0 73 60 157 535.2 0 157 61 182 535.2 0 182 62 262 535.2 0 262 63 63 535.2 0 63 64 115 535.2 0 115 65 74 535.2 0 74 66 83 535.2 0 83 67 116 535.2 0 116 68 83 535.2 0 83 69 74 535.2 0 74 70 132 535.2 0 132 71 164 535.2 0 164 72 174 535.2 0 174 73 634 535.2 0 634 74 1067 535.2 0 1067 75 1057 535.2 0 1057 76 204 535.2 0 204 77 2564 535.2 0 2564 78 3772 535.2 0 3772 79 1826 535.2 0 1826 80 57 535.2 0 57 81 87 535.2 0 87 82 79 535.2 0 79 83 71 535.2 0 71 84 80 535.2 0 80 85 50 535.2 0 50 86 102 535.2 0 102 87 69 535.2 0 69 88 76 535.2 0 76 89 87 535.2 0 87 90 79 535.2 0 79 91 68 535.2 0 68 92 91 535.2 0 91 93 74 535.2 0 74 94 81 535.2 0 81 95 67 535.2 0 67 96 86 535.2 0 86 97 97 535.2 0 97 98 66 535.2 0 66 99 76 535.2 0 76 100 67 535.2 0 67 101 71 535.2 0 71 102 63 535.2 0 63 103 62 535.2 0 62 104 73 535.2 0 73 105 75 535.2 0 75 106 268 535.2 0 268 107 163 535.2 0 163 108 191 535.2 0 191 109 77 535.2 0 77 110 57 535.2 0 57 111 69 535.2 0 69 112 61 535.2 0 61 113 76 535.2 0 76 114 164 535.2 0 164 115 113 535.2 0 113 116 157 535.2 0 157 117 78 535.2 0 78 118 77 535.2 0 77 119 52 535.2 0 52 120 78 535.2 0 78 121 67 535.2 0 67 122 77 535.2 0 77 123 61 535.2 0 61 124 71 535.2 0 71 125 85 535.2 0 85 126 100 535.2 0 100 127 227 535.2 0 227 128 270 535.2 0 270 129 405 535.2 0 405 130 358 535.2 0 358 131 99 535.2 0 99 132 82 535.2 0 82 133 125 535.2 0 125 134 185 535.2 0 185 135 304 535.2 0 304 136 286 535.2 0 286 137 138 535.2 0 138 138 88 535.2 0 88 139 113 535.2 0 113 140 116 535.2 0 116 141 70 535.2 0 70 142 60 535.2 0 60 143 69 535.2 0 69 144 75 535.2 0 75 145 143 535.2 0 143 146 249 535.2 0 249 147 635 535.2 0 635 148 494 535.2 0 494 149 99 535.2 0 99 150 75 535.2 0 75 151 56 535.2 0 56 152 59 535.2 0 59 153 82 535.2 0 82 154 106 535.2 0 106 155 471 535.2 0 471 156 140 535.2 0 140 157 143 535.2 0 143 158 89 535.2 0 89 159 139 535.2 0 139 160 246 535.2 0 246 161 208 535.2 0 208 162 79 535.2 0 79 163 92 535.2 0 92 164 186 535.2 0 186 165 286 535.2 0 286 166 304 535.2 0 304 167 123 535.2 0 123 168 99 535.2 0 99 169 70 535.2 0 70 170 86 535.2 0 86 171 81 535.2 0 81 172 81 535.2 0 81 173 66 535.2 0 66 174 78 535.2 0 78 175 83 535.2 0 83 176 50 535.2 0 50 177 55 535.2 0 55 178 86 535.2 0 86 179 121 535.2 0 121 180 109 535.2 0 109 181 89 535.2 0 89 182 84 535.2 0 84 183 91 535.2 0 91 184 248 535.2 0 248 185 361 535.2 0 361 186 520 535.2 0 520 187 171 535.2 0 171 188 87 535.2 0 87 189 82 535.2 0 82 190 85 535.2 0 85 191 77 535.2 0 77 192 116 535.2 0 116 193 95 535.2 0 95 194 95 535.2 0 95 195 331 535.2 0 331 196 500 535.2 0 500 197 456 535.2 0 456 198 174 535.2 0 174 199 109 535.2 0 109 200 86 535.2 0 86 201 81 535.2 0 81 202 76 535.2 0 76 203 85 535.2 0 85 204 94 535.2 0 94 205 66 535.2 0 66 206 87 535.2 0 87 207 94 535.2 0 94 208 103 535.2 0 103 209 93 535.2 0 93 210 141 535.2 0 141 211 108 535.2 0 108 212 156 535.2 0 156 213 132 535.2 0 132 214 125 535.2 0 125 215 321 535.2 0 321 216 467 535.2 0 467 217 391 535.2 0 391 218 109 535.2 0 109 219 134 535.2 0 134 220 128 535.2 0 128 221 118 535.2 0 118 222 83 535.2 0 83 223 85 535.2 0 85 224 86 535.2 0 86 225 200 535.2 0 200 226 244 535.2 0 244 227 153 535.2 0 153 228 76 535.2 0 76 229 77 535.2 0 77 230 111 535.2 0 111 231 118 535.2 0 118 232 112 535.2 0 112 233 128 535.2 0 128 234 201 535.2 0 201 235 373 535.2 0 373 236 2481 535.2 0 2481 237 2490 535.2 0 2490 238 2331 535.2 0 2331 239 129 535.2 0 129 240 134 535.2 0 134 241 101 535.2 0 101 242 115 535.2 0 115 243 93 535.2 0 93 244 103 535.2 0 103 245 192 535.2 0 192 246 348 535.2 0 348 247 608 535.2 0 608 248 522 535.2 0 522 249 224 535.2 0 224 250 185 535.2 0 185 251 220 535.2 0 220 252 274 535.2 0 274 253 358 535.2 0 358 254 321 535.2 0 321 255 326 535.2 0 326 256 121 535.2 0 121 257 113 535.2 0 113 258 108 535.2 0 108 259 102 535.2 0 102 260 159 535.2 0 159 261 95 535.2 0 95 262 94 535.2 0 94 263 144 535.2 0 144 264 109 535.2 0 109 265 117 535.2 0 117 266 95 535.2 0 95 267 86 535.2 0 86 268 129 535.2 0 129 269 99 535.2 0 99 270 104 535.2 0 104 271 95 535.2 0 95 272 127 535.2 0 127 273 161 535.2 0 161 274 216 535.2 0 216 275 146 535.2 0 146 276 92 535.2 0 92 277 110 535.2 0 110 278 198 535.2 0 198 279 146 535.2 0 146 280 213 535.2 0 213 281 233 535.2 0 233 282 336 535.2 0 336 283 286 535.2 0 286 284 376 535.2 0 376 285 455 535.2 0 455 286 493 535.2 0 493 287 180 535.2 0 180 288 126 535.2 0 126 289 164 535.2 0 164 290 147 535.2 0 147 291 171 535.2 0 171 292 141 535.2 0 141 293 237 535.2 0 237 294 304 535.2 0 304 295 432 535.2 0 432 296 917 535.2 0 917 297 974 535.2 0 974 298 962 535.2 0 962 299 432 535.2 0 432 300 366 535.2 0 366 301 713 535.2 0 713 Finished in 2.23 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 51,126 Reads with adapters: 9,213 (18.0%) Reads that were too short: 157 (0.3%) Reads written (passing filters): 9,056 (17.7%) Total basepairs processed: 11,671,177 bp Total written (filtered): 2,437,735 bp (20.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9213 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 5 5 16 90 0.0 3 0 70 9 11 17 8361 0.0 3 7489 690 142 40 18 33 0.0 3 4 21 6 2 19 86 0.0 3 28 16 20 22 20 13 0.0 3 0 0 0 13 21 2 0.0 3 0 0 1 1 23 8 0.0 3 0 0 1 7 25 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 27 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 33 3 0.0 3 0 0 1 2 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 39 57 0.0 3 0 0 1 56 43 5 0.0 3 0 0 0 5 44 1 0.0 3 0 0 0 1 45 36 0.0 3 0 0 2 34 46 2 0.0 3 0 0 0 2 47 2 0.0 3 0 0 0 2 48 4 0.0 3 0 0 1 3 49 5 0.0 3 0 0 0 5 50 1 0.0 3 0 0 0 1 51 9 0.0 3 0 0 1 8 52 5 0.0 3 0 0 0 5 53 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 11 0.0 3 0 0 0 11 59 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 7 0.0 3 0 0 0 7 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 5 0.0 3 0 0 0 5 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 80 4 0.0 3 0 0 0 4 88 4 0.0 3 0 0 0 4 89 4 0.0 3 0 0 0 4 91 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 94 7 0.0 3 0 0 0 7 95 2 0.0 3 0 0 1 1 96 2 0.0 3 0 0 0 2 97 4 0.0 3 0 0 2 2 98 11 0.0 3 0 0 0 11 99 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 1 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 5 0.0 3 0 0 0 5 114 143 0.0 3 0 0 0 143 115 53 0.0 3 0 0 1 52 116 3 0.0 3 0 0 0 3 117 20 0.0 3 0 0 2 18 118 17 0.0 3 0 0 2 15 119 4 0.0 3 0 0 0 4 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 1 1 122 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 137 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 1 139 4 0.0 3 0 0 0 4 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 2 0.0 3 0 0 0 2 144 4 0.0 3 0 0 1 3 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 165 11 0.0 3 0 0 0 11 166 3 0.0 3 0 0 0 3 167 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 174 5 0.0 3 0 0 0 5 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 1 183 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 215 5 0.0 3 0 0 0 5 216 7 0.0 3 0 0 0 7 217 2 0.0 3 0 0 1 1 219 3 0.0 3 0 0 0 3 220 3 0.0 3 0 0 0 3 221 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 9,056 Reads with adapters: 35 (0.4%) Reads written (passing filters): 9,056 (100.0%) Total basepairs processed: 2,437,735 bp Total written (filtered): 2,437,629 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 40.0% G: 25.7% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 34 141.5 0 34 4 1 35.4 0 1