This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.48 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 58,499 (2.7%) Reads that were too short: 19,686 (0.9%) Reads written (passing filters): 38,813 (1.8%) Total basepairs processed: 659,880,795 bp Total written (filtered): 9,329,609 bp (1.4%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 58499 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1283 535.2 0 1283 7 7704 535.2 0 7704 8 9169 535.2 0 9169 9 59 535.2 0 59 10 171 535.2 0 171 11 55 535.2 0 55 12 69 535.2 0 69 13 59 535.2 0 59 14 58 535.2 0 58 15 71 535.2 0 71 16 61 535.2 0 61 17 55 535.2 0 55 18 47 535.2 0 47 19 56 535.2 0 56 20 49 535.2 0 49 21 50 535.2 0 50 22 51 535.2 0 51 23 57 535.2 0 57 24 41 535.2 0 41 25 55 535.2 0 55 26 65 535.2 0 65 27 45 535.2 0 45 28 50 535.2 0 50 29 71 535.2 0 71 30 77 535.2 0 77 31 38 535.2 0 38 32 52 535.2 0 52 33 77 535.2 0 77 34 50 535.2 0 50 35 58 535.2 0 58 36 52 535.2 0 52 37 48 535.2 0 48 38 53 535.2 0 53 39 65 535.2 0 65 40 66 535.2 0 66 41 49 535.2 0 49 42 75 535.2 0 75 43 72 535.2 0 72 44 47 535.2 0 47 45 58 535.2 0 58 46 102 535.2 0 102 47 74 535.2 0 74 48 52 535.2 0 52 49 75 535.2 0 75 50 58 535.2 0 58 51 53 535.2 0 53 52 76 535.2 0 76 53 104 535.2 0 104 54 69 535.2 0 69 55 83 535.2 0 83 56 122 535.2 0 122 57 93 535.2 0 93 58 86 535.2 0 86 59 72 535.2 0 72 60 105 535.2 0 105 61 106 535.2 0 106 62 285 535.2 0 285 63 217 535.2 0 217 64 80 535.2 0 80 65 50 535.2 0 50 66 78 535.2 0 78 67 71 535.2 0 71 68 55 535.2 0 55 69 60 535.2 0 60 70 86 535.2 0 86 71 93 535.2 0 93 72 79 535.2 0 79 73 350 535.2 0 350 74 606 535.2 0 606 75 490 535.2 0 490 76 96 535.2 0 96 77 606 535.2 0 606 78 906 535.2 0 906 79 466 535.2 0 466 80 52 535.2 0 52 81 108 535.2 0 108 82 85 535.2 0 85 83 37 535.2 0 37 84 47 535.2 0 47 85 62 535.2 0 62 86 73 535.2 0 73 87 46 535.2 0 46 88 71 535.2 0 71 89 71 535.2 0 71 90 60 535.2 0 60 91 60 535.2 0 60 92 58 535.2 0 58 93 63 535.2 0 63 94 50 535.2 0 50 95 42 535.2 0 42 96 64 535.2 0 64 97 82 535.2 0 82 98 72 535.2 0 72 99 80 535.2 0 80 100 70 535.2 0 70 101 77 535.2 0 77 102 55 535.2 0 55 103 59 535.2 0 59 104 68 535.2 0 68 105 85 535.2 0 85 106 283 535.2 0 283 107 214 535.2 0 214 108 215 535.2 0 215 109 94 535.2 0 94 110 70 535.2 0 70 111 64 535.2 0 64 112 91 535.2 0 91 113 85 535.2 0 85 114 108 535.2 0 108 115 87 535.2 0 87 116 166 535.2 0 166 117 93 535.2 0 93 118 82 535.2 0 82 119 58 535.2 0 58 120 84 535.2 0 84 121 75 535.2 0 75 122 58 535.2 0 58 123 75 535.2 0 75 124 78 535.2 0 78 125 76 535.2 0 76 126 68 535.2 0 68 127 231 535.2 0 231 128 243 535.2 0 243 129 397 535.2 0 397 130 380 535.2 0 380 131 73 535.2 0 73 132 57 535.2 0 57 133 90 535.2 0 90 134 134 535.2 0 134 135 266 535.2 0 266 136 195 535.2 0 195 137 106 535.2 0 106 138 62 535.2 0 62 139 76 535.2 0 76 140 84 535.2 0 84 141 68 535.2 0 68 142 63 535.2 0 63 143 62 535.2 0 62 144 70 535.2 0 70 145 113 535.2 0 113 146 214 535.2 0 214 147 579 535.2 0 579 148 417 535.2 0 417 149 90 535.2 0 90 150 66 535.2 0 66 151 60 535.2 0 60 152 61 535.2 0 61 153 68 535.2 0 68 154 95 535.2 0 95 155 478 535.2 0 478 156 146 535.2 0 146 157 146 535.2 0 146 158 75 535.2 0 75 159 53 535.2 0 53 160 78 535.2 0 78 161 94 535.2 0 94 162 63 535.2 0 63 163 56 535.2 0 56 164 87 535.2 0 87 165 113 535.2 0 113 166 132 535.2 0 132 167 78 535.2 0 78 168 42 535.2 0 42 169 64 535.2 0 64 170 71 535.2 0 71 171 48 535.2 0 48 172 50 535.2 0 50 173 33 535.2 0 33 174 56 535.2 0 56 175 40 535.2 0 40 176 34 535.2 0 34 177 60 535.2 0 60 178 61 535.2 0 61 179 49 535.2 0 49 180 44 535.2 0 44 181 53 535.2 0 53 182 41 535.2 0 41 183 50 535.2 0 50 184 94 535.2 0 94 185 148 535.2 0 148 186 166 535.2 0 166 187 77 535.2 0 77 188 50 535.2 0 50 189 51 535.2 0 51 190 51 535.2 0 51 191 57 535.2 0 57 192 70 535.2 0 70 193 72 535.2 0 72 194 60 535.2 0 60 195 195 535.2 0 195 196 196 535.2 0 196 197 153 535.2 0 153 198 73 535.2 0 73 199 71 535.2 0 71 200 49 535.2 0 49 201 52 535.2 0 52 202 43 535.2 0 43 203 52 535.2 0 52 204 75 535.2 0 75 205 51 535.2 0 51 206 65 535.2 0 65 207 46 535.2 0 46 208 53 535.2 0 53 209 73 535.2 0 73 210 71 535.2 0 71 211 52 535.2 0 52 212 67 535.2 0 67 213 67 535.2 0 67 214 68 535.2 0 68 215 137 535.2 0 137 216 263 535.2 0 263 217 175 535.2 0 175 218 69 535.2 0 69 219 64 535.2 0 64 220 70 535.2 0 70 221 51 535.2 0 51 222 63 535.2 0 63 223 90 535.2 0 90 224 56 535.2 0 56 225 101 535.2 0 101 226 169 535.2 0 169 227 71 535.2 0 71 228 54 535.2 0 54 229 77 535.2 0 77 230 72 535.2 0 72 231 111 535.2 0 111 232 77 535.2 0 77 233 114 535.2 0 114 234 187 535.2 0 187 235 345 535.2 0 345 236 3362 535.2 0 3362 237 3418 535.2 0 3418 238 3158 535.2 0 3158 239 80 535.2 0 80 240 70 535.2 0 70 241 69 535.2 0 69 242 61 535.2 0 61 243 45 535.2 0 45 244 62 535.2 0 62 245 91 535.2 0 91 246 159 535.2 0 159 247 291 535.2 0 291 248 256 535.2 0 256 249 106 535.2 0 106 250 63 535.2 0 63 251 63 535.2 0 63 252 84 535.2 0 84 253 85 535.2 0 85 254 81 535.2 0 81 255 78 535.2 0 78 256 59 535.2 0 59 257 53 535.2 0 53 258 41 535.2 0 41 259 56 535.2 0 56 260 183 535.2 0 183 261 48 535.2 0 48 262 47 535.2 0 47 263 63 535.2 0 63 264 59 535.2 0 59 265 63 535.2 0 63 266 54 535.2 0 54 267 55 535.2 0 55 268 50 535.2 0 50 269 56 535.2 0 56 270 55 535.2 0 55 271 36 535.2 0 36 272 91 535.2 0 91 273 65 535.2 0 65 274 91 535.2 0 91 275 43 535.2 0 43 276 62 535.2 0 62 277 78 535.2 0 78 278 102 535.2 0 102 279 86 535.2 0 86 280 91 535.2 0 91 281 112 535.2 0 112 282 191 535.2 0 191 283 159 535.2 0 159 284 132 535.2 0 132 285 149 535.2 0 149 286 116 535.2 0 116 287 66 535.2 0 66 288 60 535.2 0 60 289 98 535.2 0 98 290 55 535.2 0 55 291 80 535.2 0 80 292 67 535.2 0 67 293 95 535.2 0 95 294 111 535.2 0 111 295 123 535.2 0 123 296 321 535.2 0 321 297 326 535.2 0 326 298 287 535.2 0 287 299 146 535.2 0 146 300 127 535.2 0 127 301 297 535.2 0 297 Finished in 1.93 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 38,813 Reads with adapters: 10,220 (26.3%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 10,207 (26.3%) Total basepairs processed: 9,329,609 bp Total written (filtered): 2,783,701 bp (29.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10220 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 5 0.0 3 0 0 5 19 149 0.0 3 0 143 5 0 1 20 9139 0.0 4 8973 163 2 1 21 33 0.0 4 8 25 22 838 0.0 4 820 14 3 1 23 12 0.0 4 1 4 0 6 1 26 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 1 46 1 0.0 4 0 0 0 0 1 57 14 0.0 4 0 0 0 0 14 60 1 0.0 4 0 0 0 0 1 61 8 0.0 4 0 0 0 0 8 101 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 154 3 0.0 4 0 0 0 0 3 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 173 1 0.0 4 1 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 1 235 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 10,207 Reads with adapters: 1,201 (11.8%) Reads written (passing filters): 10,207 (100.0%) Total basepairs processed: 2,783,701 bp Total written (filtered): 2,770,273 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1201 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 3.3% G: 92.6% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 159.5 0 9 4 12 39.9 0 12 9 84 0.0 0 2 82 10 82 0.0 1 5 77 11 1009 0.0 1 10 999 14 1 0.0 1 0 1 151 1 0.0 1 0 1 170 2 0.0 1 2 173 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.