This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.09 s (26 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 146,227 (6.7%) Reads that were too short: 92,798 (4.2%) Reads written (passing filters): 53,429 (2.4%) Total basepairs processed: 659,880,795 bp Total written (filtered): 11,366,048 bp (1.7%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 146227 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 758 535.2 0 758 7 393 535.2 0 393 8 10256 535.2 0 10256 9 9241 535.2 0 9241 10 144 535.2 0 144 11 105 535.2 0 105 12 57 535.2 0 57 13 84 535.2 0 84 14 83 535.2 0 83 15 39 535.2 0 39 16 49 535.2 0 49 17 63 535.2 0 63 18 46 535.2 0 46 19 36 535.2 0 36 20 72 535.2 0 72 21 85 535.2 0 85 22 53 535.2 0 53 23 37 535.2 0 37 24 64 535.2 0 64 25 47 535.2 0 47 26 56 535.2 0 56 27 38 535.2 0 38 28 39 535.2 0 39 29 56 535.2 0 56 30 59 535.2 0 59 31 74 535.2 0 74 32 60 535.2 0 60 33 50 535.2 0 50 34 50 535.2 0 50 35 44 535.2 0 44 36 39 535.2 0 39 37 55 535.2 0 55 38 98 535.2 0 98 39 49 535.2 0 49 40 45 535.2 0 45 41 55 535.2 0 55 42 47 535.2 0 47 43 60 535.2 0 60 44 73 535.2 0 73 45 47 535.2 0 47 46 56 535.2 0 56 47 109 535.2 0 109 48 90 535.2 0 90 49 45 535.2 0 45 50 63 535.2 0 63 51 54 535.2 0 54 52 59 535.2 0 59 53 63 535.2 0 63 54 36 535.2 0 36 55 44 535.2 0 44 56 45 535.2 0 45 57 70 535.2 0 70 58 74 535.2 0 74 59 48 535.2 0 48 60 55 535.2 0 55 61 60 535.2 0 60 62 60 535.2 0 60 63 43 535.2 0 43 64 39 535.2 0 39 65 48 535.2 0 48 66 55 535.2 0 55 67 63 535.2 0 63 68 95 535.2 0 95 69 84 535.2 0 84 70 107 535.2 0 107 71 47 535.2 0 47 72 71 535.2 0 71 73 95 535.2 0 95 74 124 535.2 0 124 75 162 535.2 0 162 76 54 535.2 0 54 77 85 535.2 0 85 78 94 535.2 0 94 79 59 535.2 0 59 80 54 535.2 0 54 81 42 535.2 0 42 82 73 535.2 0 73 83 62 535.2 0 62 84 45 535.2 0 45 85 61 535.2 0 61 86 61 535.2 0 61 87 61 535.2 0 61 88 64 535.2 0 64 89 56 535.2 0 56 90 63 535.2 0 63 91 115 535.2 0 115 92 68 535.2 0 68 93 55 535.2 0 55 94 76 535.2 0 76 95 70 535.2 0 70 96 62 535.2 0 62 97 201 535.2 0 201 98 133 535.2 0 133 99 116 535.2 0 116 100 43 535.2 0 43 101 70 535.2 0 70 102 50 535.2 0 50 103 57 535.2 0 57 104 61 535.2 0 61 105 50 535.2 0 50 106 102 535.2 0 102 107 113 535.2 0 113 108 108 535.2 0 108 109 69 535.2 0 69 110 58 535.2 0 58 111 88 535.2 0 88 112 100 535.2 0 100 113 83 535.2 0 83 114 69 535.2 0 69 115 111 535.2 0 111 116 106 535.2 0 106 117 136 535.2 0 136 118 141 535.2 0 141 119 128 535.2 0 128 120 155 535.2 0 155 121 166 535.2 0 166 122 95 535.2 0 95 123 136 535.2 0 136 124 205 535.2 0 205 125 3966 535.2 0 3966 126 389 535.2 0 389 127 466 535.2 0 466 128 119 535.2 0 119 129 105 535.2 0 105 130 123 535.2 0 123 131 86 535.2 0 86 132 95 535.2 0 95 133 86 535.2 0 86 134 235 535.2 0 235 135 274 535.2 0 274 136 308 535.2 0 308 137 127 535.2 0 127 138 120 535.2 0 120 139 120 535.2 0 120 140 83 535.2 0 83 141 90 535.2 0 90 142 109 535.2 0 109 143 75 535.2 0 75 144 108 535.2 0 108 145 209 535.2 0 209 146 458 535.2 0 458 147 527 535.2 0 527 148 121 535.2 0 121 149 102 535.2 0 102 150 117 535.2 0 117 151 125 535.2 0 125 152 127 535.2 0 127 153 97 535.2 0 97 154 133 535.2 0 133 155 169 535.2 0 169 156 157 535.2 0 157 157 278 535.2 0 278 158 221 535.2 0 221 159 243 535.2 0 243 160 157 535.2 0 157 161 181 535.2 0 181 162 170 535.2 0 170 163 151 535.2 0 151 164 167 535.2 0 167 165 250 535.2 0 250 166 225 535.2 0 225 167 240 535.2 0 240 168 117 535.2 0 117 169 105 535.2 0 105 170 120 535.2 0 120 171 135 535.2 0 135 172 181 535.2 0 181 173 845 535.2 0 845 174 1423 535.2 0 1423 175 1089 535.2 0 1089 176 637 535.2 0 637 177 109 535.2 0 109 178 129 535.2 0 129 179 136 535.2 0 136 180 143 535.2 0 143 181 483 535.2 0 483 182 728 535.2 0 728 183 750 535.2 0 750 184 277 535.2 0 277 185 224 535.2 0 224 186 897 535.2 0 897 187 844 535.2 0 844 188 1198 535.2 0 1198 189 263 535.2 0 263 190 127 535.2 0 127 191 124 535.2 0 124 192 124 535.2 0 124 193 170 535.2 0 170 194 172 535.2 0 172 195 137 535.2 0 137 196 132 535.2 0 132 197 98 535.2 0 98 198 80 535.2 0 80 199 143 535.2 0 143 200 158 535.2 0 158 201 222 535.2 0 222 202 140 535.2 0 140 203 168 535.2 0 168 204 158 535.2 0 158 205 178 535.2 0 178 206 393 535.2 0 393 207 712 535.2 0 712 208 846 535.2 0 846 209 265 535.2 0 265 210 98 535.2 0 98 211 178 535.2 0 178 212 270 535.2 0 270 213 235 535.2 0 235 214 252 535.2 0 252 215 390 535.2 0 390 216 212 535.2 0 212 217 199 535.2 0 199 218 260 535.2 0 260 219 266 535.2 0 266 220 428 535.2 0 428 221 405 535.2 0 405 222 396 535.2 0 396 223 383 535.2 0 383 224 472 535.2 0 472 225 5535 535.2 0 5535 226 11106 535.2 0 11106 227 9331 535.2 0 9331 228 238 535.2 0 238 229 521 535.2 0 521 230 1458 535.2 0 1458 231 2990 535.2 0 2990 232 2628 535.2 0 2628 233 481 535.2 0 481 234 255 535.2 0 255 235 230 535.2 0 230 236 134 535.2 0 134 237 161 535.2 0 161 238 138 535.2 0 138 239 121 535.2 0 121 240 179 535.2 0 179 241 209 535.2 0 209 242 242 535.2 0 242 243 198 535.2 0 198 244 172 535.2 0 172 245 240 535.2 0 240 246 258 535.2 0 258 247 230 535.2 0 230 248 183 535.2 0 183 249 194 535.2 0 194 250 657 535.2 0 657 251 1143 535.2 0 1143 252 1226 535.2 0 1226 253 1466 535.2 0 1466 254 1490 535.2 0 1490 255 867 535.2 0 867 256 96 535.2 0 96 257 116 535.2 0 116 258 126 535.2 0 126 259 149 535.2 0 149 260 164 535.2 0 164 261 225 535.2 0 225 262 194 535.2 0 194 263 219 535.2 0 219 264 200 535.2 0 200 265 260 535.2 0 260 266 145 535.2 0 145 267 191 535.2 0 191 268 284 535.2 0 284 269 243 535.2 0 243 270 181 535.2 0 181 271 271 535.2 0 271 272 252 535.2 0 252 273 356 535.2 0 356 274 294 535.2 0 294 275 489 535.2 0 489 276 296 535.2 0 296 277 298 535.2 0 298 278 478 535.2 0 478 279 1257 535.2 0 1257 280 4943 535.2 0 4943 281 4225 535.2 0 4225 282 3219 535.2 0 3219 283 736 535.2 0 736 284 347 535.2 0 347 285 523 535.2 0 523 286 290 535.2 0 290 287 244 535.2 0 244 288 194 535.2 0 194 289 354 535.2 0 354 290 428 535.2 0 428 291 316 535.2 0 316 292 299 535.2 0 299 293 191 535.2 0 191 294 292 535.2 0 292 295 548 535.2 0 548 296 660 535.2 0 660 297 865 535.2 0 865 298 1119 535.2 0 1119 299 1408 535.2 0 1408 300 2675 535.2 0 2675 301 10553 535.2 0 10553 Finished in 2.23 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 53,429 Reads with adapters: 11,330 (21.2%) Reads that were too short: 152 (0.3%) Reads written (passing filters): 11,178 (20.9%) Total basepairs processed: 11,366,048 bp Total written (filtered): 3,034,161 bp (26.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11330 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 11 0.0 3 0 0 5 6 16 121 0.0 3 0 101 8 12 17 10185 0.0 3 9295 707 146 37 18 37 0.0 3 7 18 1 11 19 61 0.0 3 2 7 28 24 20 453 0.0 3 388 30 7 28 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 1 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 6 0.0 3 0 0 0 6 35 1 0.0 3 0 0 0 1 36 8 0.0 3 0 0 1 7 37 6 0.0 3 0 0 1 5 38 2 0.0 3 0 0 0 2 39 1 0.0 3 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 23 0.0 3 0 0 2 21 48 1 0.0 3 0 0 0 1 49 6 0.0 3 0 0 1 5 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 58 5 0.0 3 0 0 0 5 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 65 2 0.0 3 0 0 0 2 66 145 0.0 3 0 0 1 144 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 9 0.0 3 0 0 0 9 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 5 0.0 3 0 0 0 5 100 8 0.0 3 0 0 0 8 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 6 0.0 3 0 0 0 6 106 4 0.0 3 0 0 0 4 107 4 0.0 3 0 0 0 4 110 4 0.0 3 0 0 0 4 114 1 0.0 3 0 0 0 1 115 5 0.0 3 0 0 0 5 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 119 79 0.0 3 0 0 4 75 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 1 136 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 1 159 2 0.0 3 0 0 0 2 160 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 167 7 0.0 3 0 0 0 7 168 4 0.0 3 0 0 0 4 169 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 1 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 207 4 0.0 3 0 0 0 4 217 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 11,178 Reads with adapters: 49 (0.4%) Reads written (passing filters): 11,178 (100.0%) Total basepairs processed: 3,034,161 bp Total written (filtered): 3,034,009 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 49 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.0% C: 14.3% G: 26.5% T: 8.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 45 174.7 0 45 4 3 43.7 0 3 5 1 10.9 0 1