This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 56.79 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 154,967 (7.1%) Reads that were too short: 108,380 (4.9%) Reads written (passing filters): 46,587 (2.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 10,723,256 bp (1.6%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 154967 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 642 535.2 0 642 7 333 535.2 0 333 8 9825 535.2 0 9825 9 11080 535.2 0 11080 10 104 535.2 0 104 11 96 535.2 0 96 12 53 535.2 0 53 13 85 535.2 0 85 14 113 535.2 0 113 15 65 535.2 0 65 16 59 535.2 0 59 17 100 535.2 0 100 18 140 535.2 0 140 19 144 535.2 0 144 20 79 535.2 0 79 21 80 535.2 0 80 22 56 535.2 0 56 23 41 535.2 0 41 24 87 535.2 0 87 25 71 535.2 0 71 26 56 535.2 0 56 27 60 535.2 0 60 28 59 535.2 0 59 29 45 535.2 0 45 30 64 535.2 0 64 31 91 535.2 0 91 32 78 535.2 0 78 33 63 535.2 0 63 34 68 535.2 0 68 35 54 535.2 0 54 36 46 535.2 0 46 37 72 535.2 0 72 38 103 535.2 0 103 39 53 535.2 0 53 40 71 535.2 0 71 41 63 535.2 0 63 42 56 535.2 0 56 43 41 535.2 0 41 44 63 535.2 0 63 45 71 535.2 0 71 46 197 535.2 0 197 47 202 535.2 0 202 48 102 535.2 0 102 49 50 535.2 0 50 50 73 535.2 0 73 51 45 535.2 0 45 52 85 535.2 0 85 53 57 535.2 0 57 54 41 535.2 0 41 55 50 535.2 0 50 56 52 535.2 0 52 57 78 535.2 0 78 58 76 535.2 0 76 59 53 535.2 0 53 60 52 535.2 0 52 61 53 535.2 0 53 62 67 535.2 0 67 63 31 535.2 0 31 64 50 535.2 0 50 65 55 535.2 0 55 66 64 535.2 0 64 67 49 535.2 0 49 68 67 535.2 0 67 69 67 535.2 0 67 70 65 535.2 0 65 71 55 535.2 0 55 72 78 535.2 0 78 73 52 535.2 0 52 74 114 535.2 0 114 75 117 535.2 0 117 76 48 535.2 0 48 77 74 535.2 0 74 78 97 535.2 0 97 79 60 535.2 0 60 80 34 535.2 0 34 81 38 535.2 0 38 82 69 535.2 0 69 83 54 535.2 0 54 84 53 535.2 0 53 85 78 535.2 0 78 86 56 535.2 0 56 87 55 535.2 0 55 88 70 535.2 0 70 89 65 535.2 0 65 90 72 535.2 0 72 91 118 535.2 0 118 92 59 535.2 0 59 93 73 535.2 0 73 94 76 535.2 0 76 95 72 535.2 0 72 96 61 535.2 0 61 97 120 535.2 0 120 98 79 535.2 0 79 99 81 535.2 0 81 100 55 535.2 0 55 101 67 535.2 0 67 102 63 535.2 0 63 103 86 535.2 0 86 104 45 535.2 0 45 105 82 535.2 0 82 106 93 535.2 0 93 107 89 535.2 0 89 108 67 535.2 0 67 109 84 535.2 0 84 110 53 535.2 0 53 111 71 535.2 0 71 112 79 535.2 0 79 113 73 535.2 0 73 114 68 535.2 0 68 115 69 535.2 0 69 116 117 535.2 0 117 117 132 535.2 0 132 118 104 535.2 0 104 119 107 535.2 0 107 120 157 535.2 0 157 121 101 535.2 0 101 122 99 535.2 0 99 123 121 535.2 0 121 124 183 535.2 0 183 125 3405 535.2 0 3405 126 346 535.2 0 346 127 424 535.2 0 424 128 73 535.2 0 73 129 79 535.2 0 79 130 75 535.2 0 75 131 66 535.2 0 66 132 70 535.2 0 70 133 65 535.2 0 65 134 116 535.2 0 116 135 179 535.2 0 179 136 134 535.2 0 134 137 76 535.2 0 76 138 95 535.2 0 95 139 105 535.2 0 105 140 66 535.2 0 66 141 97 535.2 0 97 142 82 535.2 0 82 143 67 535.2 0 67 144 78 535.2 0 78 145 129 535.2 0 129 146 277 535.2 0 277 147 347 535.2 0 347 148 88 535.2 0 88 149 64 535.2 0 64 150 94 535.2 0 94 151 103 535.2 0 103 152 102 535.2 0 102 153 88 535.2 0 88 154 92 535.2 0 92 155 108 535.2 0 108 156 96 535.2 0 96 157 163 535.2 0 163 158 128 535.2 0 128 159 172 535.2 0 172 160 81 535.2 0 81 161 70 535.2 0 70 162 108 535.2 0 108 163 137 535.2 0 137 164 127 535.2 0 127 165 117 535.2 0 117 166 126 535.2 0 126 167 138 535.2 0 138 168 77 535.2 0 77 169 91 535.2 0 91 170 91 535.2 0 91 171 128 535.2 0 128 172 164 535.2 0 164 173 583 535.2 0 583 174 807 535.2 0 807 175 555 535.2 0 555 176 278 535.2 0 278 177 79 535.2 0 79 178 114 535.2 0 114 179 112 535.2 0 112 180 107 535.2 0 107 181 341 535.2 0 341 182 551 535.2 0 551 183 590 535.2 0 590 184 234 535.2 0 234 185 117 535.2 0 117 186 277 535.2 0 277 187 332 535.2 0 332 188 327 535.2 0 327 189 180 535.2 0 180 190 115 535.2 0 115 191 95 535.2 0 95 192 101 535.2 0 101 193 121 535.2 0 121 194 109 535.2 0 109 195 72 535.2 0 72 196 96 535.2 0 96 197 66 535.2 0 66 198 54 535.2 0 54 199 89 535.2 0 89 200 92 535.2 0 92 201 135 535.2 0 135 202 107 535.2 0 107 203 139 535.2 0 139 204 120 535.2 0 120 205 74 535.2 0 74 206 180 535.2 0 180 207 224 535.2 0 224 208 280 535.2 0 280 209 125 535.2 0 125 210 90 535.2 0 90 211 162 535.2 0 162 212 301 535.2 0 301 213 185 535.2 0 185 214 232 535.2 0 232 215 304 535.2 0 304 216 132 535.2 0 132 217 139 535.2 0 139 218 280 535.2 0 280 219 279 535.2 0 279 220 308 535.2 0 308 221 238 535.2 0 238 222 278 535.2 0 278 223 308 535.2 0 308 224 437 535.2 0 437 225 6306 535.2 0 6306 226 14084 535.2 0 14084 227 11866 535.2 0 11866 228 149 535.2 0 149 229 624 535.2 0 624 230 588 535.2 0 588 231 1376 535.2 0 1376 232 577 535.2 0 577 233 243 535.2 0 243 234 127 535.2 0 127 235 150 535.2 0 150 236 66 535.2 0 66 237 104 535.2 0 104 238 85 535.2 0 85 239 79 535.2 0 79 240 92 535.2 0 92 241 100 535.2 0 100 242 124 535.2 0 124 243 82 535.2 0 82 244 122 535.2 0 122 245 118 535.2 0 118 246 147 535.2 0 147 247 150 535.2 0 150 248 107 535.2 0 107 249 135 535.2 0 135 250 659 535.2 0 659 251 1383 535.2 0 1383 252 737 535.2 0 737 253 618 535.2 0 618 254 580 535.2 0 580 255 348 535.2 0 348 256 85 535.2 0 85 257 100 535.2 0 100 258 115 535.2 0 115 259 91 535.2 0 91 260 88 535.2 0 88 261 101 535.2 0 101 262 112 535.2 0 112 263 132 535.2 0 132 264 162 535.2 0 162 265 347 535.2 0 347 266 183 535.2 0 183 267 182 535.2 0 182 268 232 535.2 0 232 269 177 535.2 0 177 270 161 535.2 0 161 271 208 535.2 0 208 272 279 535.2 0 279 273 223 535.2 0 223 274 303 535.2 0 303 275 870 535.2 0 870 276 287 535.2 0 287 277 160 535.2 0 160 278 206 535.2 0 206 279 480 535.2 0 480 280 1805 535.2 0 1805 281 1429 535.2 0 1429 282 1048 535.2 0 1048 283 467 535.2 0 467 284 673 535.2 0 673 285 1132 535.2 0 1132 286 405 535.2 0 405 287 461 535.2 0 461 288 346 535.2 0 346 289 481 535.2 0 481 290 832 535.2 0 832 291 645 535.2 0 645 292 598 535.2 0 598 293 309 535.2 0 309 294 489 535.2 0 489 295 893 535.2 0 893 296 1221 535.2 0 1221 297 1977 535.2 0 1977 298 2358 535.2 0 2358 299 3372 535.2 0 3372 300 6606 535.2 0 6606 301 28701 535.2 0 28701 Finished in 2.25 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 46,587 Reads with adapters: 11,307 (24.3%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 11,290 (24.2%) Total basepairs processed: 10,723,256 bp Total written (filtered): 3,070,130 bp (28.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11307 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 6 0.0 3 0 0 6 19 130 0.0 3 0 128 2 20 11095 0.0 4 10946 146 3 21 27 0.0 4 0 27 22 6 0.0 4 2 0 4 23 10 0.0 4 0 0 0 7 3 24 2 0.0 4 0 0 0 2 27 1 0.0 4 1 39 1 0.0 4 0 1 49 5 0.0 4 0 0 0 0 5 50 4 0.0 4 0 0 0 0 4 53 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 183 2 0.0 4 0 0 1 0 1 187 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 1 189 2 0.0 4 0 1 0 0 1 228 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 11,290 Reads with adapters: 1,266 (11.2%) Reads written (passing filters): 11,290 (100.0%) Total basepairs processed: 3,070,130 bp Total written (filtered): 3,057,201 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1266 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 3.7% G: 93.5% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 176.4 0 1 4 4 44.1 0 4 6 2 2.8 0 2 8 1 0.2 0 1 9 332 0.0 0 13 319 10 828 0.0 1 25 803 11 90 0.0 1 2 88 13 1 0.0 1 0 1 36 1 0.0 1 0 1 42 2 0.0 1 2 69 1 0.0 1 1 72 1 0.0 1 1 150 1 0.0 1 0 1 208 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.