This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.30 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,192,295 Reads with adapters: 136,324 (6.2%) Reads that were too short: 23,544 (1.1%) Reads written (passing filters): 112,780 (5.1%) Total basepairs processed: 659,880,795 bp Total written (filtered): 30,795,141 bp (4.7%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 136324 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1790 535.2 0 1790 7 48541 535.2 0 48541 8 40377 535.2 0 40377 9 84 535.2 0 84 10 67 535.2 0 67 11 66 535.2 0 66 12 58 535.2 0 58 13 72 535.2 0 72 14 59 535.2 0 59 15 48 535.2 0 48 16 55 535.2 0 55 17 59 535.2 0 59 18 50 535.2 0 50 19 62 535.2 0 62 20 53 535.2 0 53 21 72 535.2 0 72 22 74 535.2 0 74 23 72 535.2 0 72 24 44 535.2 0 44 25 39 535.2 0 39 26 80 535.2 0 80 27 47 535.2 0 47 28 56 535.2 0 56 29 61 535.2 0 61 30 68 535.2 0 68 31 55 535.2 0 55 32 59 535.2 0 59 33 68 535.2 0 68 34 46 535.2 0 46 35 57 535.2 0 57 36 54 535.2 0 54 37 65 535.2 0 65 38 119 535.2 0 119 39 281 535.2 0 281 40 702 535.2 0 702 41 1377 535.2 0 1377 42 66 535.2 0 66 43 62 535.2 0 62 44 47 535.2 0 47 45 75 535.2 0 75 46 60 535.2 0 60 47 55 535.2 0 55 48 30 535.2 0 30 49 51 535.2 0 51 50 48 535.2 0 48 51 98 535.2 0 98 52 125 535.2 0 125 53 61 535.2 0 61 54 68 535.2 0 68 55 66 535.2 0 66 56 37 535.2 0 37 57 58 535.2 0 58 58 65 535.2 0 65 59 46 535.2 0 46 60 60 535.2 0 60 61 65 535.2 0 65 62 46 535.2 0 46 63 42 535.2 0 42 64 66 535.2 0 66 65 67 535.2 0 67 66 67 535.2 0 67 67 56 535.2 0 56 68 60 535.2 0 60 69 55 535.2 0 55 70 71 535.2 0 71 71 85 535.2 0 85 72 100 535.2 0 100 73 73 535.2 0 73 74 115 535.2 0 115 75 48 535.2 0 48 76 66 535.2 0 66 77 73 535.2 0 73 78 64 535.2 0 64 79 70 535.2 0 70 80 53 535.2 0 53 81 56 535.2 0 56 82 60 535.2 0 60 83 89 535.2 0 89 84 95 535.2 0 95 85 72 535.2 0 72 86 79 535.2 0 79 87 56 535.2 0 56 88 67 535.2 0 67 89 67 535.2 0 67 90 79 535.2 0 79 91 123 535.2 0 123 92 131 535.2 0 131 93 162 535.2 0 162 94 70 535.2 0 70 95 81 535.2 0 81 96 74 535.2 0 74 97 96 535.2 0 96 98 386 535.2 0 386 99 663 535.2 0 663 100 586 535.2 0 586 101 98 535.2 0 98 102 122 535.2 0 122 103 90 535.2 0 90 104 74 535.2 0 74 105 75 535.2 0 75 106 61 535.2 0 61 107 85 535.2 0 85 108 74 535.2 0 74 109 101 535.2 0 101 110 87 535.2 0 87 111 338 535.2 0 338 112 313 535.2 0 313 113 251 535.2 0 251 114 90 535.2 0 90 115 93 535.2 0 93 116 86 535.2 0 86 117 81 535.2 0 81 118 73 535.2 0 73 119 62 535.2 0 62 120 92 535.2 0 92 121 82 535.2 0 82 122 72 535.2 0 72 123 88 535.2 0 88 124 112 535.2 0 112 125 111 535.2 0 111 126 69 535.2 0 69 127 71 535.2 0 71 128 58 535.2 0 58 129 68 535.2 0 68 130 81 535.2 0 81 131 58 535.2 0 58 132 88 535.2 0 88 133 105 535.2 0 105 134 103 535.2 0 103 135 98 535.2 0 98 136 98 535.2 0 98 137 128 535.2 0 128 138 106 535.2 0 106 139 159 535.2 0 159 140 82 535.2 0 82 141 70 535.2 0 70 142 96 535.2 0 96 143 135 535.2 0 135 144 127 535.2 0 127 145 103 535.2 0 103 146 140 535.2 0 140 147 150 535.2 0 150 148 133 535.2 0 133 149 80 535.2 0 80 150 102 535.2 0 102 151 86 535.2 0 86 152 80 535.2 0 80 153 89 535.2 0 89 154 86 535.2 0 86 155 91 535.2 0 91 156 97 535.2 0 97 157 86 535.2 0 86 158 193 535.2 0 193 159 156 535.2 0 156 160 154 535.2 0 154 161 67 535.2 0 67 162 98 535.2 0 98 163 122 535.2 0 122 164 140 535.2 0 140 165 83 535.2 0 83 166 96 535.2 0 96 167 98 535.2 0 98 168 95 535.2 0 95 169 117 535.2 0 117 170 134 535.2 0 134 171 174 535.2 0 174 172 223 535.2 0 223 173 152 535.2 0 152 174 81 535.2 0 81 175 55 535.2 0 55 176 92 535.2 0 92 177 184 535.2 0 184 178 236 535.2 0 236 179 246 535.2 0 246 180 180 535.2 0 180 181 109 535.2 0 109 182 108 535.2 0 108 183 298 535.2 0 298 184 376 535.2 0 376 185 314 535.2 0 314 186 129 535.2 0 129 187 125 535.2 0 125 188 150 535.2 0 150 189 206 535.2 0 206 190 137 535.2 0 137 191 137 535.2 0 137 192 148 535.2 0 148 193 96 535.2 0 96 194 90 535.2 0 90 195 126 535.2 0 126 196 114 535.2 0 114 197 81 535.2 0 81 198 64 535.2 0 64 199 115 535.2 0 115 200 93 535.2 0 93 201 105 535.2 0 105 202 97 535.2 0 97 203 118 535.2 0 118 204 117 535.2 0 117 205 80 535.2 0 80 206 95 535.2 0 95 207 107 535.2 0 107 208 91 535.2 0 91 209 104 535.2 0 104 210 154 535.2 0 154 211 169 535.2 0 169 212 199 535.2 0 199 213 145 535.2 0 145 214 388 535.2 0 388 215 796 535.2 0 796 216 593 535.2 0 593 217 181 535.2 0 181 218 82 535.2 0 82 219 94 535.2 0 94 220 168 535.2 0 168 221 115 535.2 0 115 222 209 535.2 0 209 223 799 535.2 0 799 224 853 535.2 0 853 225 633 535.2 0 633 226 95 535.2 0 95 227 105 535.2 0 105 228 166 535.2 0 166 229 182 535.2 0 182 230 124 535.2 0 124 231 100 535.2 0 100 232 97 535.2 0 97 233 90 535.2 0 90 234 76 535.2 0 76 235 86 535.2 0 86 236 125 535.2 0 125 237 145 535.2 0 145 238 401 535.2 0 401 239 327 535.2 0 327 240 392 535.2 0 392 241 139 535.2 0 139 242 176 535.2 0 176 243 122 535.2 0 122 244 72 535.2 0 72 245 102 535.2 0 102 246 93 535.2 0 93 247 122 535.2 0 122 248 85 535.2 0 85 249 120 535.2 0 120 250 111 535.2 0 111 251 130 535.2 0 130 252 145 535.2 0 145 253 138 535.2 0 138 254 174 535.2 0 174 255 94 535.2 0 94 256 180 535.2 0 180 257 176 535.2 0 176 258 190 535.2 0 190 259 146 535.2 0 146 260 200 535.2 0 200 261 189 535.2 0 189 262 124 535.2 0 124 263 308 535.2 0 308 264 458 535.2 0 458 265 357 535.2 0 357 266 109 535.2 0 109 267 117 535.2 0 117 268 77 535.2 0 77 269 113 535.2 0 113 270 134 535.2 0 134 271 260 535.2 0 260 272 1607 535.2 0 1607 273 999 535.2 0 999 274 1351 535.2 0 1351 275 152 535.2 0 152 276 209 535.2 0 209 277 383 535.2 0 383 278 467 535.2 0 467 279 181 535.2 0 181 280 224 535.2 0 224 281 113 535.2 0 113 282 139 535.2 0 139 283 287 535.2 0 287 284 448 535.2 0 448 285 549 535.2 0 549 286 319 535.2 0 319 287 110 535.2 0 110 288 131 535.2 0 131 289 96 535.2 0 96 290 94 535.2 0 94 291 94 535.2 0 94 292 109 535.2 0 109 293 96 535.2 0 96 294 102 535.2 0 102 295 94 535.2 0 94 296 84 535.2 0 84 297 137 535.2 0 137 298 240 535.2 0 240 299 391 535.2 0 391 300 288 535.2 0 288 301 191 535.2 0 191 Finished in 5.32 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 112,780 Reads with adapters: 49,218 (43.6%) Reads that were too short: 127 (0.1%) Reads written (passing filters): 49,091 (43.5%) Total basepairs processed: 30,795,141 bp Total written (filtered): 13,568,302 bp (44.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49218 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 13 0.0 2 0 0 0 13 15 35 0.0 3 0 0 21 14 16 354 0.0 3 0 280 48 26 17 47938 0.0 3 43572 3425 738 203 18 158 0.0 3 11 120 11 16 19 154 0.0 3 0 0 74 80 20 234 0.0 3 0 0 0 234 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 1 26 1 0.0 3 0 1 29 1 0.0 3 0 0 0 1 30 3 0.0 3 0 0 0 3 31 3 0.0 3 1 0 0 2 33 1 0.0 3 1 40 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 1 3 60 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 72 1 0.0 3 0 0 0 1 73 16 0.0 3 0 0 2 14 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 7 0.0 3 0 0 0 7 80 19 0.0 3 0 0 0 19 81 5 0.0 3 0 0 0 5 83 12 0.0 3 0 0 0 12 84 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 0 3 97 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 8 0.0 3 0 0 0 8 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 45 0.0 3 0 0 1 44 133 6 0.0 3 0 0 0 6 134 3 0.0 3 0 0 1 2 135 9 0.0 3 0 0 1 8 136 5 0.0 3 0 0 0 5 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 3 0.0 3 0 0 0 3 153 6 0.0 3 0 0 0 6 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 161 3 0.0 3 0 0 0 3 164 2 0.0 3 0 0 0 2 165 2 0.0 3 0 1 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 14 0.0 3 0 0 0 14 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 1 1 228 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 245 3 0.0 3 0 0 1 2 246 1 0.0 3 0 0 0 1 248 3 0.0 3 0 0 0 3 249 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 253 2 0.0 3 0 0 0 2 254 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 1 270 3 0.0 3 0 0 0 3 275 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 2 0.0 3 0 0 0 2 281 12 0.0 3 0 0 0 12 282 8 0.0 3 0 0 0 8 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.72 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 49,091 Reads with adapters: 223 (0.5%) Reads written (passing filters): 49,091 (100.0%) Total basepairs processed: 13,568,302 bp Total written (filtered): 13,567,604 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 223 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.7% C: 50.2% G: 22.0% T: 12.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 209 767.0 0 209 4 6 191.8 0 6 5 5 47.9 0 5 6 2 12.0 0 2 10 1 0.0 1 0 1